Period Of Evolution Research Articles

Photometric and Spectroscopic Analysis of the SX Phe Star BL Cam

In the present paper, we report the photometric and spectroscopic observations obtained by the 1.88 m telescope at the Kottamia astronomical observatory of the pulsating star BL Cam. Fourier analysis of the light curves reveals that the fundamental mode has two harmonics. The O-C method is used to establish the period changes. So far, the analysis has been very successful in mapping the pulsation amplitude of the star across the instability strip. By using the formalism of Eddington and Plakidis (1929), we found significant results and strong indications of the evolutionary period change. A total of 55 new maximum light timings are reported. New values of (1/P) dP/dt are estimated using the O-C diagram based on all newly obtained times of maximum light combined with those taken from the literature, assuming the periods are decreasing and changing smoothly. To compute the effective temperature and surface gravity of the star, we performed model atmosphere analysis on its spectra. The physical parameters of the star are calculated and compared with the evolutionary models.

Evolution of helium bubbles induced by helium irradiation in polycrystalline palladium: An insight into tritium aging

Essential insight into evolution principles of helium (He) bubbles within Palladium (Pd), a prominent hydrogen isotope storage and tritium handling material, is of great importance to understand the service performance of such transition metal in the field of nuclear industry. Particularly, the features of He bubbles and their evolution details have long been attracting broad interests. Herein, He bubbles with different structural features and corresponding distinct evolving processes within polycrystalline Pd were investigated by transmission electron microscopy (TEM), elastic recoil detection (ERD) and thermal desorption spectroscopy (TDS). He bubbles were induced and regulated by irradiation conditions. Comprehensive characterizing results indicate that bubbles with small sizes, spherical shape and uniform distribution, realized by low-fluence irradiation, are inclined to be stable in Pd matrix, while coarsened bubbles with faceted shape and regional distribution in the high-fluence condition are prone to release at relatively low temperatures. Meanwhile the evolving processes of He bubbles including migration, coarsening and releasing within polycrystalline Pd were analyzed. The impact of the structural characteristics such as surfaces or grain boundaries of Pd, acted as “fast path” for bubble diffusion, coarsening and release, was systematically discussed. Moreover, the correlation between tuned irradiation and actual tritium aging was discussed by analyzing He concentrations and bubble sizes in both processes. The study reveals important features of He bubble evolution in Pd, which will provide positive reference for understanding correlative behaviors in other irradiation conditions, especially tritium aging. • He bubbles with different features were obtained by tuning fluence and energy of He + irradiation. • Evolution behaviors of He bubbles including nucleation, migration and coarsening were studied by TEM, ERD and TDS. • The stability of He bubble is closely related to the size that small one inclines to stabilize while large one tends to release. • Trapping and coarsening of He bubbles are promoted in grain boundaries and structural defects. • Different evolution periods of He bubble in this study were analyzed for the purpose to offering an analogous reference for tritium aging.

A potential role of salicylic acid in the evolutionary behavior of Trichoderma as a plant pathogen: from Marchantia polymorpha to Arabidopsis thaliana

Main conclusionRecognition of the interaction of Trichoderma during the evolution of land plants plays a potential key role in the development of the salicylic acid defense pathway and the establishment of a mutualistic relationship.Marchantia polymorpha is a common liverwort considered in recent years as a model plant for evolutionary studies on plant–microorganism interactions. Despite the lack of research, remarkable results have been reported regarding the understanding of metabolic and evolutionary processes of beneficial and/or harmful interactions, owing to a better understanding of the origin and evolution of different plant defense pathways. In this study, we have carried out work on the direct and indirect interactions (exudates and volatiles) of M. polymorpha with different species of the fungal genus Trichoderma. These interactions showed different outcomes, including resistance or even growth promotion and disease. We have analyzed the level of tissue colonization and defense-related gene expression. Furthermore, we have used the pteridophyte Dryopteris affinis and the angiosperm Arabidopsis thaliana, as subsequent steps in plant evolution, together with the plant pathogen Rhizoctonia solani as a control of plant pathogenicity. Trichoderma virens, T. brevicompactum and T. hamatum are pathogens of M. polymorpha, while exudates of T. asperellum are harmful to the plant. The analysis of the expression of several defense genes in M. polymorpha and A. thaliana showed that there is a correlation of the transcriptional activation of SA-related genes with resistance or susceptibility of M. polymorpha to Trichoderma. Moreover, exogenous SA provides resistance to the virulent Trichoderma species. This beneficial fungus may have had an evolutionary period of interaction with plants in which it behaved as a plant pathogen until plants developed a defense system to limit its colonization through a defense response mediated by SA.

Genome-wide identification, comprehensive characterization of transcription factors, cis-regulatory elements, protein homology, and protein interaction network of DREB gene family in Solanum lycopersicum.

Tomato is a drought-sensitive crop which has high susceptibility to adverse climatic changes. Dehydration-responsive element-binding (DREB) are significant plant transcription factors that have a vital role in regulating plant abiotic stress tolerance by networking with DRE/CRT cis-regulatory elements in response to stresses. In this study, bioinformatics analysis was performed to conduct the genome-wide identification and characterization of DREB genes and promoter elements in Solanum lycopersicum. In genome-wide coverage, 58 SlDREB genes were discovered on 12 chromosomes that justified the criteria of the presence of AP2 domain as conserved motifs. Intron-exon organization and motif analysis showed consistency with phylogenetic analysis and confirmed the absence of the A3 class, thus dividing the SlDREB genes into five categories. Gene expansion was observed through tandem duplication and segmental duplication gene events in SlDREB genes. Ka/Ks values were calculated in ortholog pairs that indicated divergence time and occurrence of purification selection during the evolutionary period. Synteny analysis demonstrated that 32 out of 58 and 47 out of 58 SlDREB genes were orthologs to Arabidopsis and Solanum tuberosum, respectively. Subcellular localization predicted that SlDREB genes were present in the nucleus and performed primary functions in DNA binding to regulate the transcriptional processes according to gene ontology. Cis-acting regulatory element analysis revealed the presence of 103 motifs in 2.5-kbp upstream promoter sequences of 58 SlDREB genes. Five representative SlDREB proteins were selected from the resultant DREB subgroups for 3D protein modeling through the Phyre2 server. All models confirmed about 90% residues in the favorable region through Ramachandran plot analysis. Moreover, active catalytic sites and occurrence in disorder regions indicated the structural and functional flexibility of SlDREB proteins. Protein association networks through STRING software suggested the potential interactors that belong to different gene families and are involved in regulating similar functional and biological processes. Transcriptome data analysis has revealed that the SlDREB gene family is engaged in defense response against drought and heat stress conditions in tomato. Overall, this comprehensive research reveals the identification and characterization of SlDREB genes that provide potential knowledge for improving abiotic stress tolerance in tomato.

Evolution of Homo in the Middle and Late Pleistocene

The Middle and Late Pleistocene is arguably the most interesting period in human evolution. This broad period witnessed the evolution of our own lineage, as well as that of our sister taxon, the Neanderthals, and related Denisovans. It is exceptionally rich in both fossil and archaeological remains, and uniquely benefits from insights gained through molecular approaches, such as paleogenetics and paleoproteomics, that are currently not widely applicable in earlier contexts. This wealth of information paints a highly complex picture, often described as ‘the Muddle in the Middle,’ defying the common adage that ‘more evidence is needed’ to resolve it. Here we review competing phylogenetic scenarios and the historical and theoretical developments that shaped our approaches to the fossil record, as well as some of the many remaining open questions associated with this period. We propose that advancing our understanding of this critical time requires more than the addition of data and will necessitate a major shift in our conceptual and theoretical framework.

Hitting reset: Industry evolution, generational technology cycles, and the dynamic value of firm experience

AbstractResearch SummaryWe study how the value of depth and breadth of experience shifts across generational technology cycles and industry evolution. Experience breadth builds organizational flexibility that is valuable during technology transitions and when the industry is younger, while experience depth builds domain‐specific resources that are important during periods of technology stability and later periods of industry evolution. We test our theory in the console game industry, where publishers face repeated periods of transition and stability across multiple console generations. We find support for most hypotheses, except that the value of breadth correlates with the magnitude of any transition instead of decreasing linearly over time. We contribute to research on the changing value of firm experience and to the understanding of generational technological change during industry evolution.Managerial AbstractMany industries face generational changes. How incumbent firms handle those transitions is important to firm survival and success. We study how the prior experience of video game publishers helps them navigate transitions. First, we find that firms with deep experience in a genre produce more successful games in that genre only during stable periods in the industry. Second, the benefits of deep experience increase over time as some of the experience‐built capabilities of the firm survive technological transitions. Third, broad and diversified firms perform best during generational transitions as they successfully enter new domains made popular by the generational shift. We suggest that the product strategies of video game firms should depend on the relevant experience they possess and the degree of environmental change.

Exploration of the genomic atlas of Dof transcription factor family across genus Oryza provides novel insights on rice breeding in changing climate.

DNA-binding with one finger (Dof) transcription factors have been demonstrated to regulate various stresses and developmental processes in plants. Their identification and comparative evolutionary analyses in cultivated and wild species of genus oryza were yet to be explored. In this context, we report a comprehensive genomics atlas of DNA-binding with one finger (Dof) family genes in 13 diverse rice genomes (five cultivated and eight rice wild-relatives) through a genome-wide scanning approach. A galore of 238 Dof genes, identified across the genus Oryza, are categorized into seven distinct subgroups by comparative phylogenetic analysis with the model plant Arabidopsis. Conserved motifs and gene structure analyses unveiled the prevalence of species- and subgroups-specific structural and functional diversity that is expediating with the evolutionary period. Our results indicate that Dof genes might have undergone strong purifying selections and segmental duplications to expand their gene family members in corresponding Oryza genomes. We speculate that miR2927 potentially targets the Dof domain to regulate gene expression under different climatic conditions, which are supported by in-silico and wet-lab experiments-based expression profiles. In a nutshell, we report several superior haplotypes significantly associated with early flowering in a treasure trove of 3,010 sequenced rice accessions and have validated these haplotypes with two years of field evaluation-based flowering data of a representative subpanel. Finally, we have provided some insights on the resolution of Oryza species phylogeny discordance and divergence highlighting the mosaic evolutionary history of the genus Oryza. Overall, this study reports a complete genomic landscape of the Dof family in cultivated and wild Oryza species that could greatly facilitate in fast-track development of early maturing and climate-resilient rice cultivars through modern haplotype-led breeding.

Numerical investigation on the cloud cavitating flow over a Clark Y foil under free surface effect

For a high-speed vehicle in the presence of cavitating flow while operating near the free surface, the interaction between the cavitating flow and the free surface can be very critical. In this paper, the cloud cavitating flow over a Clark Y foil near the free surface was investigated via numerical approaches. The large eddy simulation (LES) turbulence model, the volume of fluid (VOF) method, as well as the Schnerr Sauer cavitation model were used in the simulation to solve for the cloud cavitation flow region and capture the interphase between the multiphase (air, water and vapour). The analysis centred on the results of the two- (2D) and three-dimensional (3D) cases for the Clark Y foil at various submerged depths for several evolution cycles. The free surface affects the cavity profile, evolution period, re-entrant jet inside/beneath the cavity and hydrodynamic coefficients. The cavity has become shorter and thicker under the free surface effect. Induced by the re-entrant jet thickness, the cavity becomes more stable as the submerged depth decreases. The cavity evolution, the cavity length and thickness of different cycles are similar, while the shedding cavity can delay the evolution. A similar phenomenon can be observed in 3D, but the flow structure is more complex than in 2D. The 3D effect likewise manifests as shortening the cavity length while changing the direction of the re-entrant jet inside/beneath the cavity.

Editorial

There is no difficulty to accept the claim that our civil society has entered a period in human evolution which is considered by the increase in the rate of velocity of our history associated with uncertainty in its route. The present situation has the opportunity to expand further as well as it is creating a deeper crisis and contradictions along with severe uncertainties and risks for the human being. It is very much true that globalization has eliminated several physical and psychological barriers among people and countries by transferring power which has contributed the empowerment of civil society and decentralization of power.

Research progress on the relationship between leaf senescence and quality, yield and stress resistance in horticultural plants.

Leaf senescence, the final stage of leaf development, is one of the adaptive mechanisms formed by plants over a long period of evolution. Leaf senescence is accompanied by various changes in cell structure, physiological metabolism, and gene expressions. This process is controlled by a variety of internal and external factors. Meanwhile, the genes and plant hormones involved in leaf aging affect the quality, yield and stress resistance in horticultural plants. Leaf senescence mediated by plant hormones affected plant quality at both pre-harvest and post-harvest stages. Exogenous plant growth regulators or plant hormone inhibitors has been applied to delay leaf senescence. Modification of related gene expression by over-expression or antisense inhibition could delay or accelerate leaf senescence, and thus influence quality. Environmental factors such as light, temperature and water status also trigger or delay leaf senescence. Delaying leaf senescence could increase chloroplast lifespan and photosynthesis and thus improve source strength, leading to enhanced yield. Accelerating leaf senescence promotes nutrient redistribution from old leaves into young leaves, and may raise yield under certain circumstances. Many genes and transcriptional factors involved in leaf senescence are associated with responses to abiotic and biotic stresses. WRKY transcriptional factors play a vital role in this process and they could interact with JA signalling. This review summarized how genes, plant hormones and environmental factors affect the quality, yield. Besides, the regulation of leaf senescence holds great promise to improving the resistance to plant biotic and abiotic stresses.

Continuity of patient care and patient control with high blood pressure at the first stage of medical attention

Objective: to compare the blood pressure numbers of patients with high blood pressure with and without continuity of care at the first stage of medical attention. Methods: a multicenter cross-sectional study was conducted in three family medicine units in a public institution. A family medicine information system was used to review the electronic files of patients with high blood pressure from July 2018 to June 2019. The systematic sample was used to complete the size of sample 358 for each group of patients, with and without continuity of medical care. It was determined that continuity existed when the continuity of care index was ≥ 0.7, getting also clinical variables. Results: of 701 electronic files of patients with high blood pressure, there was an average continuity of care index of 0.68 ± 0.23, without differences between the group of patients, with and without continuity of care, with variables: control of high blood pressure, evolution period of high blood pressure, number of patients that were assisted in the emergency departments due to uncontrolled hypertension, antihypertensive medications used and high blood pressure control. Conclusions: There was found, no relation between continuity of patient care and high blood pressure control.

Genome-Wide Identification of the AGC Protein Kinase Gene Family Related to Photosynthesis in Rice (Oryza sativa).

The cAMP-dependent protein kinase A, cGMP-dependent protein kinase G and phospholipid-dependent protein kinase C (AGC) perform various functions in plants, involving growth, immunity, apoptosis and stress response. AGC gene family is well described in Arabidopsis, however, limited information is provided about AGC genes in rice, an important cereal crop. This research studied the AGC gene family in the AA genome species: Oryza sativa ssp. japonica, Oryza sativa ssp. indica, Oryza nivara, Oryza rufipogon, Oryza glaberrima, Oryza meridionalis, Oryza barthii, Oryza glumaepatula and Oryza longistaminata were searched and classified into six subfamilies, and it was found that these species have similar numbers of members. The analysis of gene duplication and selection pressure indicated that the AGC gene family expanded mainly by segmental or whole genome duplication (WGD), with purifying selection during the long evolutionary period. RNA-seq analysis revealed that OsAGCs of subfamily V were specifically highly expressed in leaves, and the expression patterns of these genes were compared with that of photosynthesis-related genes using qRT-PCR, discovered that OsAGC9, OsAGC20, and OsAGC22 might participate in photosynthesis. These results provide an informative perspective for exploring the evolutionary of AGC gene family and its practical application in rice.

Responding to warming in polar oceans: A commentary on Molina et al.(2022).

Antarctic marine species live in one of the most thermally stable environments on Earth. They have evolved in these cold stable conditions for many millions of years. The long period for evolution, the isolation and mixing of populations produced by glacial cycles and the environmental heterogeneity in terms of light, productivity and physical disturbance, has produced a diverse fauna with an estimated 20 000 species, or more, living on the seabed. It has also produced a fauna that is possibly the most sensitive to warming on Earth in an environment that is changing faster than most, if not all, others. There is a great need to understand this threatened biodiversity and to find ways to mitigate the future prospects of species loss in this special environment that supports unique biology including the only vertebrate species on Earth that live without haemoglobin.

Paleoproterozoic (ca. 2.1–1.9 Ga) dioritic-granitic magmatism in the Dunhuang Terrane, Northwestern China: Implications for petrogenesis and tectonic evolution

The implications of petrogenesis and tectonics of Paleoproterozoic (ca. 2.1–1.9 Ga) granitoid rocks in Dunhuang Terrane have hitherto received less attention. According to data from zircon UPb dating, the ages of dioritic gneiss, granodioritic gneiss and potassic granite are 2045 ± 44 Ma, 1998 ± 15 Ma and 1950 ± 38 Ma, respectively. The dioritic gneiss has MgO = 1.87–2.93 wt% (Mg# = 46.8–50.9), Yb ≤ 1.9 ppm and Y ≤ 18 ppm, with variable εHf(t) (−2.8 − +5.5) and εNd(t) (−5.6 − +1.3) values, indicating that the mantle wedge source above the subducted slab contributed with subduction fluids and ocean sediment melt in an arc setting. The granodioritic gneiss has relatively high MgO (3.00–1.83 wt%), variable Cr (13.5–106 ppm) and Ni (12.2–72.9 ppm), negative εHf(t) (−20.4 to −10.1) and εNd(t) (−15.2 to −12.8) values, and high P2O5 and TiO2 saturation temperatures (∼800 °C). These data indicate magma source of late Paleo- to early Mesoarchean (∼3.2–3.1 Ga) continental crust rocks. The potassic granite has high K2O (5.52–6.79 wt%) and low MgO (0.10–0.19 wt%) concentrations, low Mg# (37.7–44.6) values, and negative εHf(t) (−12.8 to −2.5) and εNd(t) (−10.6 to −4.8) values. These results suggest that pre-existing Neoarchean crustal rocks were partially melted possibly under transitional stage from syn- to post-collision. Compared with the adjacent areas, Paleoproterozoic (ca. 2.1–1.8 Ga) evolutionary period related to assembly of Columbia supercontinent in Dunhuang Terrane is comparable with that in northeastern Tarim Craton and southwestern Alxa Block, but terminated ∼100 Ma later than that in southeastern Tarim Craton (Aketashtage Terrane).

Research Trends in Groundwater and Stable Isotopes

Groundwater is essential in the management of water resources globally. The water quality of aquifers is affected by climate change and population growth, aspects that can be addressed with stable isotope analysis. This study aims to carry out an analysis of the scientific information related to groundwater and stable isotopes (GSI) using scientific databases (Scopus and Web of Science) to evaluate the intellectual structure of the subject and the emerging research lines. The methodology includes: (i) topic search selection, (ii) tools in databases processing, (iii) bibliometric analysis, and (iv) review by clustering technique. The results showed that the scientific production of GSI can be addressed through three evolution periods: I (1969–1990), II (1991–2005), and III (2006–2021). Periods I and II did not significantly contribute to publications because, in the past, most of the student’s thesis (M.Sc. and Ph.D) consisted of writing a report that summarizes their works. Therefore, the researcher was not obliged to publish their results in a professional journal. Finally, the third period showed exponential growth, representing 82.34% of the total publications in this theme because, in the last years, institutions require at least one scientific article depending on the country and university, in order to graduate with an M.Sc. and PhD. Finally, the contribution of this study is reflected in the recognition of new research lines and their applicability by the knowledge of recharge sources, environmental aspects, infiltration, knowledge of the aquifer-meteoric water system, and groundwater-superficial water interaction. These aspects offer the possibility of analyzing integrated water resources management at the watershed or river-aquifer systems level.

Experimental Evolution Shows Body Size Decrease in Response to Hypoxia, with a Complex Effect on Plastic Size Response to Temperature.

AbstractThere is a scientific debate whether oxygen concentration may be a factor driving the pattern of size decrease at higher temperature. Central to this debate is the fact that oxygen availability relative to demand for living organisms decreases with increasing temperature. We examined whether rotifers Lecane inermis exposed to hypoxic conditions would evolve smaller sizes than rotifers exposed to normoxic conditions, using experimental evolution with the same fluctuating temperature but differentiated by three regimes of oxygen availability: normoxia, hypoxia throughout the whole thermal range, and hypoxia only at the highest temperature. Immediately after the six-month experiment (more than 90 generations), we tested the plasticity of size responses to temperature in three post-evolution groups, and we related these responses to fitness. The results show that normoxic rotifers had evolved significantly larger sizes than two hypoxic rotifer groups, which were similar in size. All three groups displayed similar plastic body size reductions in response to warming over the range of temperatures they were exposed to during the period of experimental evolution, but they showed different and complex responses at two temperatures below this range. Any type of plastic response to different temperatures resulted in a similar fitness pattern across post-evolution groups. We conclude that (i) these rotifers showed a genetic basis for the pattern of size decrease following evolution under both temperature-dependent and temperature-independent hypoxia; and (ii) plastic body size responds consistently to temperatures that are within the thermal range that the rotifers experienced during their evolutionary history, but responses become more noisy at novel temperatures, suggesting the importance of evolutionary responses to reliable environmental cues.

Existence and controllability of fractional evolution inclusions with impulse and sectorial operator

Many evolutionary operations fromdiverse fields of engineering and physical sciences go through abrupt modifications of state at specific moments of time among periods of non-stop evolution. These operations are more conveniently modeled via impulsive differential equations and inclusions. In this work, firstly we address the existence of mild solutions for nonlocal fractional impulsive semilinear differential inclusions related to Caputo derivative in Banach spaces when the linear part is sectorial. Secondly, we determine the enough, conditions for the controllability of the studied control problem. We apply effectively fixed point theorems, contraction mapping, multivalued analysis and fractional calculus. Moreover, we enhance our results by introducing an illustrative examples.

Guide for interpreting and reporting luminescence dating results

The development and application of luminescence dating and dosimetry techniques have grown exponentially in the last several decades. Luminescence methods provide age control for a broad range of geological and archaeological contexts and can characterize mineral and glass properties linked to geologic origin, Earth-surface processes, and past exposure to light, heat, and ionizing radiation. The applicable age range for luminescence methods spans the last 500,000 years or more, which covers the period of modern human evolution, and provides context for rates and magnitudes of geological processes, hazards, and climate change. Given the growth in applications and publications of luminescence data, there is a need for unified, community-driven guidance regarding the publication and interpretation of luminescence results. This paper presents a guide to the essential information necessary for publishing and archiving luminescence ages as well as supporting data that is transportable and expandable for different research objectives and publication outlets. We outline the information needed for the interpretation of luminescence data sets, including data associated with equivalent dose, dose rate, age models, and stratigraphic context. A brief review of the fundamentals of luminescence techniques and applications, including guidance on sample collection and insight into laboratory processing and analysis steps, is presented to provide context for publishing and data archiving.

Recurrent but Short-Lived Duplications of Centromeric Proteins in Holocentric Caenorhabditis Species.

Centromeric histones (CenH3s) are essential for chromosome inheritance during cell division in most eukaryotes. CenH3 genes have rapidly evolved and undergone repeated gene duplications and diversification in many plant and animal species. In Caenorhabditis species, two independent duplications of CenH3 (named hcp-3 for HoloCentric chromosome-binding Protein 3) were previously identified in C. elegans and C. remanei. Using phylogenomic analyses in 32 Caenorhabditis species, we find strict retention of the ancestral hcp-3 gene and 10 independent duplications. Most hcp-3L (hcp-3-like) paralogs are only found in 1–2 species, are expressed in both males and females/hermaphrodites, and encode histone fold domains with 69–100% identity to ancestral hcp-3. We identified novel N-terminal protein motifs, including putative kinetochore protein-interacting motifs and a potential separase cleavage site, which are well conserved across Caenorhabditis HCP-3 proteins. Other N-terminal motifs vary in their retention across paralogs or species, revealing potential subfunctionalization or functional loss following duplication. An N-terminal extension in the hcp-3L gene of C. afra revealed an unprecedented protein fusion, where hcp-3L fused to duplicated segments from hcp-4 (nematode CENP-C). By extending our analyses beyond CenH3, we found gene duplications of six inner and outer kinetochore genes in Caenorhabditis, which appear to have been retained independent of hcp-3 duplications. Our findings suggest that centromeric protein duplications occur frequently in Caenorhabditis nematodes, are selectively retained for short evolutionary periods, then degenerate or are lost entirely. We hypothesize that unique challenges associated with holocentricity in Caenorhabditis may lead to this rapid “revolving door” of kinetochore protein paralogs.

Spatially and temporally resolved plasma formation on alumina target in microwave-enhanced laser-induced breakdown spectroscopy

Microwave energy was applied to the laser-induced breakdown spectroscopy (LIBS) of laser-ablated Al plasma using a helical antenna. The process of generating an enhanced laser ablation plasma was visualized with a high-speed camera. The time and space evolution of the plasma were investigated by changing the laser power, microwave energy, and microwave energy duration. The obtained raw images were converted into 8-bit 255 grayscale and binary black and white (BW) images and were analyzed using Matlab. Three major periods of plasma evolution were established based on the key physical process involved. Laser energy was the primary parameter involved in period 1, where laser ablation and initial plasma formation occurred. The microwaves had little significance at the beginning of the ablation process, as suggested by the minimal changes in plasma size and intensity. The microwaves controlled the expansion of the plasma and sustained breakdown in period 2. The post ablation breakdown of the material by microwaves sustained the enlargement of the plasma. The events leading to plasma dissipation comprised period 3, where the nonthermal plasma was sustained for 500 μs after the microwave was turned off. The effects of the laser and microwave parameters in the timeline of these periods were discussed. Complementary emission intensity measurements were also presented.