26 Al is a long-life radioactive isotope with a half lifetime of near 1 Myr. The origin of Galactic 26 Al is dominated by massive stars and their core-collapse supernovae. Detections of 1809 keV emission from 26 Al provide direct evidence that nucleosynthesis is ongoing in the Galaxy. The gamma-ray line shapes reflect the dynamics of the ejected isotopes in the interstellar medium and then probe properties of ISM and Galactic rotation effect. Gamma-ray emissions of 26 Al in the Galaxy are studied with the high spectral resolution INTEGRAL spectrometer (SPI). We carry out the first spectral survey of 26 Al gamma-ray line emission along the Galactic plane. The 26 Al line energy shifts reflect the large-scale Galactic rotation. The 26 Al intensity is brighter in the 4th than in the 1st quadrant (ratio ~ 1.3); the 26 Al line toward the direction of the Aquila region appears somewhat broadened; a latitudinal scale height of [Formula: see text] pc for 26 Al in the inner Galaxy is determined. Strong 26 Al emission signal is detected in the nearby star-formation regions Sco-Cen and Cygnus. The 26 Al line shapes in star-formation regions provide a clue to constrain the kinematic properties of ISM. In addition, we derive the flux ratio of 60 Fe /26 Al ~ 15% which can be directly compared with theoretical predictions. More theoretical work on nuclear reactions, massive star evolution models deserves improvements.