Fossil Evidence Research Articles

The Initial Upper Paleolithic from Tolbor-16, Mongolia: Characteristics of the Lithic Assemblage and Their Evolutionary Implications

According to fossil evidence, the Initial Upper Paleolithic (IUP) refers to archaeological assemblages associated with the early dispersal of Homo sapiens across Eurasia. These assemblages are often described as exhibiting a combination of Middle and Upper Paleolithic typo-technological features or, more broadly, as a technology in progression from the former to the latter. Genetic evidence indicates that as members of our species moved across Europe and Asia, they encountered Neanderthal, Denisovan, and possibly other local populations. At the Eurasian scale, the IUP shows considerable variation, but to what extent this corresponds to a relatively unified cultural package strictly associated with our species, or a suite of unrelated technologies reflecting the complex dynamics of a global population turnover, remains unclear. Like most archaeological assemblages, however, the IUP illustrates a combination of adaptive and normative behaviors, along with some stochastic variation. Here, we investigate what drives typo-technological variation within an IUP assemblage to identify traits relevant for larger scale inter-regional comparisons. Specifically, we describe a lithic assemblage dated to ca. 45 ka cal BP from the site of Tolbor-16 in northern Mongolia. We identify three defining aspects of the IUP at the site, namely variation in core shape and size, patterns of blade core reduction, and the presence of Middle Paleolithic typo-technological features. Our goal is to clarify the influence of dynamic reduction processes on assemblage composition and, within the variation observed, to identify typo-technological features that are relevant for inter-regional comparisons and scenarios of population dispersal.

Dart and the Taung juvenile: making sense of a century-old record of hominin evolution in Africa.

The announcement in 1925 by Raymond Dart of the discovery of the Taung juvenile's skull in a quarry in sub-Saharan Africa is deservedly a classic publication in the history of palaeoanthropology. Dart's paper-which designated Taung as the type specimen of the early hominin species Australopithecus africanus-provided the first fossil evidence supporting Charles Darwin's 1871 prediction that Africa was where the human lineage originated. The Taung juvenile's combination of ape and human characteristics eventually led to a paradigm shift in our understanding of human evolution. This contribution focuses on the milieu in which Dart's paper appeared (i.e. what was understood in 1925 about human evolution), the fossil evidence as set out by Dart, his interpretation of how a species represented by a fossilized juvenile's skull fitted within prevailing narratives about human evolution and the significance of the fossil being found in an environment inferred to be very different from that occupied by living apes. We also briefly review subsequent fossil finds that have corroborated the argument Dart made for having discovered evidence of a hitherto unknown close relative of humans, and summarize our current understanding of the earliest stages of human evolution and its environmental context.

A multi-ancestry GWAS meta-analysis of facial features and its application in predicting archaic human features

Facial morphology, a complex trait influenced by genetics, holds great significance in evolutionary research. However, due to limited fossil evidence, the facial characteristics of Neanderthals and Denisovans have remained largely unknown. In this study, we conducted a large-scale multi-ethnic meta-analysis of the genome-wide association study (GWAS), including 9674 East Asians and 10,115 Europeans, quantitatively assessing 78 facial traits using 3D facial images. We identified 71 genomic loci associated with facial features, including 21 novel loci. We developed a facial polygenic score (FPS) that enables the prediction of facial features based on genetic information. Interestingly, the distribution of FPSs among populations from diverse continental groups exhibited relevant correlations with observed facial features. Furthermore, we applied the FPS to predict the facial traits of seven Neanderthals and one Denisovan using ancient DNA and aligned predictions with the fossil records. Our results suggested that Neanderthals and Denisovans likely shared similar facial features, such as a wider but shorter nose and a wider endocanthion distance. The decreased mouth width was characterized specifically in Denisovans. The integration of genomic data and facial trait analysis provides valuable insights into the evolutionary history and adaptive changes in human facial morphology.

“Out-of-India” dispersal of the extinct fabaceous genus Podocarpium Braun ex Stizenberger

Podocarpium is a well-known extinct single-seeded legume genus having extensive Cenozoic fossil occurrences from East Asia to central Europe. To date, there have been no fossil records of this genus from South Asia, including India. Here, we report and describe for the first time a well-preserved fossil pod from the early Eocene of Rajasthan, western India, assigned to extinct Podocarpium based on detailed morphology. It is characterized by an asymmetrical, oblique valve with a single oblong seed, a distinctly curved stipe, a wingless margin, a prominent vascular strand along the dorsal suture that anastomoses into the apex, a weakly curved ventral suture, fine reticulate veins, a short acuminate apex, and a narrowly acute base. This discovery is the oldest fossil evidence of Podocarpium recorded to date worldwide. Together with previous fossil records, we suggest an “Out-of-India” dispersal hypothesis to explain the biogeography of this extinct legume genus. It might have originated under warm and humid tropical climate conditions in the early Eocene of India (South Asia), then migrated to China (East Asia), and subsequently spread north and westward to Europe.

Fossil evidence in the Bimbila Limestones of the Oti Group in the Voltaian Basin of Ghana

Fossil evidence in the Bimbila Limestones of the Oti Group in the Voltaian Basin of Ghana

Middle Triassic and middle Permian radiolarians from the Kamitaki Complex in the Sasayama area, Hyogo Prefecture, Southwest Japan: Evidence for Triassic plate subduction along the eastern margin of Paleo-Asia

The Kamitaki Complex, situated in the Sasayama area in Southwest Japan, has long been presumed to be a Permian subduction-related accretionary complex based on correlations from previous studies. However, because of the lack of fossil evidence, the exact age of the complex remained uncertain for a long time period. To address this gap in knowledge, a geological survey and microfossil mapping were conducted in the Kamitaki Complex to determine its age and geological context.A geological survey revealed that the Kamitaki Complex mainly consists of clastic rocks, and a mixture of sandstone, basalt, and chert blocks within the mudstones. The Kamitaki Complex is tectonically intercalated into the Lopingian (Late Permian) accretionary complex of the Ultra-Tamba Terrane and Late Triassic accretionary complex of the Tamba Terrane. The lithological and structural characteristics of the Kamitaki Complex confirm that it is an accretionary complex. Microfossil mapping yielded depositional ages, with radiolarian fauna such as Eptingium nakasekoi, Pseudostylosphaera japonica, Cryptostephanidium japonicum, and Oertlispongus cf. diacanthus identified in mudstones suggesting an Anisian (early Middle Triassic) age. In contrast, radiolarian fauna found in cherts, including Pseudoalbaillella? aff. longicornis, and Follicucullus cf. porrectus, indicate an early Capitanian (late Guadalupian, middle Permian) depositional age. These findings suggest that the Kamitaki Complex records a trenchward migration of the oceanic plate in a pelagic environment from the early Capitanian and an accretion at the trench during the Anisian period.Conventionally, the plate boundary between the Panthalassa and Paleo-Asia during the latest Permian to Middle Triassic was of the transform type, primarily because no subduction-related accretionary complexes from this period have been identified in the Japanese Islands. However, the discovery of Kamitaki Complex, an Anisian accretionary complex, provides evidence of Middle Triassic subduction activity along the eastern margin of Paleo-Asia. According to the internal structure and age polarity of the Ultra-Tamba and Tamba terranes, an accretionary complex developed over a prolonged period (approximately 120 million years) in a tectonic setting that persisted along the eastern margin of Paleo-Asia from the late Guadalupian to the earliest Cretaceous period.

Cyanobacterial and fungi-like microbial fossils from the earliest Cambrian phosphorite of South China

Microbes, as essential components of any ecosystem for their metabolic capabilities of driving energy and matter cycles, must have been omnipresent in the earliest metazoan-dominated marine ecosystem that was initially established during the Ediacaran-Cambrian transition. However, contemporaneous microbial fossils and their ecological roles are rarely known. Here we report an exquisitely preserved microbial fossil assemblage from the ∼535 million years old phosphorite of South China. Ten types of filamentous and spherical fossils have been identified and most are comparable to modern fungi, cyanobacteria, and microalgae at a cellular level. Particularly, mold- and yeast-like morphotypes that are interpreted as fungi provide potential fossil evidence for exploring the early evolution of fungi. This microbial assemblage including fungal and cyanobacterial analogues built symbiotic mats composed of decomposers and producers that were ecologically indispensable for early metazoans. A rapid phosphatization followed by silica-cementation is responsible for the high fidelity of fossil preservation, and hence is an ideal taphonomic window to explore the microbial world in geological past.

Evolution and extinction in a supercontinental world: did the breakup of Rodinia provide metazoans with evolutionary salvation?

A time discrepancy of at least 200–300 million years exists between the generally accepted onset of metazoan evolution as currently evidenced from fossils compared with that from studies at the molecular level. That temporal disparity coincides with the existence and subsequent breakup of the Rodinia Supercontinent when the earliest evolutionary crucibles were isolated intracratonic basins rather than the globally connected shallow seaways of subsequent eras. However, the discovery of fossil evidence to complement the extrapolations from molecular data has been, and continues to be, hampered, by both the geographical limitations of these isolated intracratonic basins and the fact that any such discovery would conflict with the global geological mindset that macrofossils of such age do not exist. Yet recently identified within the intracratonic Amadeus Basin of central Australia is a suite of macroscopic metazoan fossils that dates to ca 850–840 Ma, exemplifying an early attempt at animal evolution within the restrictions of the Rodinia Supercontinent. Such early metazoan evolution within such isolated crucibles, however, was extremely vulnerable to variations in climatic conditions, which threatened total extinction because the isolation of these basins limited metazoan distribution by denying them the possibility of migrating to safer havens. The Amadeus Basin evidence suggests that early metazoan evolution perhaps comprised cycles of about 10 million years when evolution flourished followed by climate change induced extinction and a subsequent evolutionary void of between 50 and 100 million years. Such cycles possibly characterised metazoan evolution until the Rodinia Supercontinent broke-up, after which connections between smaller oceans, shallow seas and narrow waterways provided metazoan life passageway to sanctuary during times of environmental stress. As the Amadeus Basin was just one of a number of isolated Rodinian-aged intracratonic basins, it is likely that similar fossils of the earliest metazoans are preserved elsewhere awaiting discovery.

The Chemistry and Biology of Lycopodium Alkaloids.

Lycopodiales, an order comprising 388 distinct species, is the source of Lycopodium alkaloids (LAs), a group of naturally occurring alkaloids that share a common biosynthesis and structural attributes. These remarkable organisms are considered vestiges of ancient ferns, with fossil evidence dating their existence back to an impressive 300 million years. LAs usually are tricyclic or tetracyclic compounds with C16N or C16N2 skeleton. But then there are also have a few C11N, C15N, C15N2, C22N2, and C27N3 skeleton. LAs have attracted much scientific attention because of their important biological activities related to acetylcholinesterase and unique structural characteristics. From 1881 to December 2023, there are 593 LAs from 49 species of Lycopodiales have been reported. Because the total amount of LAs is nearly five times that of 1994, the classification and group allocation of some newly isolated LAs is often challenging and not unambiguous by Ayer's simple classification. This review makes a more systematic and detailed classification for it and provides extensive coverage of naturally occurring LAs discovered from 1881 to December 2023. Until now, there is no comprehensively summary of biological activity of the LAs. This review is the first time covered the biological activity of the all LAs.

The evolutionary origins of the Hemichordata (Enteropneusta & Pterobranchia) - A review based on fossil evidence and interpretations.

The evolutionary origins of the Hemichordata (Enteropneusta & Pterobranchia) - A review based on fossil evidence and interpretations.

Physical constraints during Snowball Earth drive the evolution of multicellularity.

Molecular and fossil evidence suggests that complex eukaryotic multicellularity evolved during the late Neoproterozoic era, coincident with Snowball Earth glaciations, where ice sheets covered most of the globe. During this period, environmental conditions-such as seawater temperature and the availability of photosynthetically active light in the oceans-likely changed dramatically. Such changes would have had significant effects on both resource availability and optimal phenotypes. Here, we construct and apply mechanistic models to explore (i) how environmental changes during Snowball Earth and biophysical constraints generated selective pressures, and (ii) how these pressures may have had differential effects on organisms with different forms of biological organization. By testing a series of alternative-and commonly debated-hypotheses, we demonstrate how multicellularity was likely acquired differently in eukaryotes and prokaryotes owing to selective differences on their size due to the biophysical and metabolic regimes they inhabit: decreasing temperatures and resource availability instigated by the onset of glaciations generated selective pressures towards smaller sizes in organisms in the diffusive regime and towards larger sizes in motile heterotrophs. These results suggest that changing environmental conditions during Snowball Earth glaciations gave multicellular eukaryotes an evolutionary advantage, paving the way for the complex multicellular lineages that followed.

Hot spring oases in the periglacial desert as the Last Glacial Maximum refugia for temperate trees in Central Europe.

Northern glacial refugia are a hotly debated concept. The idea that many temperate organisms survived the Last Glacial Maximum (LGM; ~26.5 to 19 thousand years) in several sites across central and northern Europe stems from phylogeographic analyses, yet direct fossil evidence has thus far been missing. Here, we present the first unequivocal proof that thermophilous trees such as oak (Quercus), linden (Tilia), and common ash (Fraxinus excelsior) survived the LGM in Central Europe. The persistence of the refugium was promoted by a steady influx of hydrothermal waters that locally maintained a humid and warm microclimate. We reconstructed the geological and palaeohydrological factors responsible for the emergence of hot springs during the LGM and argue that refugia of this type, allowing the long-term survival and rapid post-LGM dispersal of temperate elements, were not exceptional in the European periglacial zone.

Cellular structure of dinosaur scales reveals retention of reptile-type skin during the evolutionary transition to feathers

Fossil feathers have transformed our understanding of integumentary evolution in vertebrates. The evolution of feathers is associated with novel skin ultrastructures, but the fossil record of these changes is poor and thus the critical transition from scaled to feathered skin is poorly understood. Here we shed light on this issue using preserved skin in the non-avian feathered dinosaur Psittacosaurus. Skin in the non-feathered, scaled torso is three-dimensionally replicated in silica and preserves epidermal layers, corneocytes and melanosomes. The morphology of the preserved stratum corneum is consistent with an original composition rich in corneous beta proteins, rather than (alpha-) keratins as in the feathered skin of birds. The stratum corneum is relatively thin in the ventral torso compared to extant quadrupedal reptiles, reflecting a reduced demand for mechanical protection in an elevated bipedal stance. The distribution of the melanosomes in the fossil skin is consistent with melanin-based colouration in extant crocodilians. Collectively, the fossil evidence supports partitioning of skin development in Psittacosaurus: a reptile-type condition in non-feathered regions and an avian-like condition in feathered regions. Retention of reptile-type skin in non-feathered regions would have ensured essential skin functions during the early, experimental stages of feather evolution.

Historical climate change and vicariance events contributed to the intercontinental disjunct distribution pattern of ash species (Fraxinus, Oleaceae)

The Northern Hemisphere temperate forests exhibit a disjunct distributional pattern in Europe, North America, and East Asia. Here, to reveal the promoter of intercontinental disjunct distribution, Fraxinus was used as a model organism to integrate abundant fossil evidence with high-resolution phylogenies in a phytogeographic analysis. We constructed a robust phylogenetic tree using genomic data, reconstructed the geographic ancestral areas, and evaluated the effect of incorporating fossil information on the reconstructed biogeographic history. The phylogenetic relationships of Fraxinus were highly resolved and divided into seven clades. Fraxinus originated in western North America during Eocene, and six intercontinental dispersal events and five intercontinental vicariance events were occured. Results suggest that climate change and vicariance contributed to the intercontinental disjunct distribution pattern of Fraxinus. Moreover, results highlight the necessity of integrating phylogenetic relationship and fossil to improve the reliability of inferred biogeographic events and our understanding of the processes underlying disjunct distributions.

Stomatal evolution and plant adaptation to future climate.

Global climate change is affecting plant photosynthesis and transpiration processes, as well as increasing weather extremes impacting socio-political and environmental events and decisions for decades to come. One major research challenge in plant biology and ecology is the interaction of photosynthesis with the environment. Stomata control plant gas exchange and their evolution was a crucial innovation that facilitated the earliest land plants to colonize terrestrial environments. Stomata couple homoiohydry, together with cuticles, intercellular gas space, with the endohydric water-conducting system, enabling plants to adapt and diversify across the planet. Plants control stomatal movement in response to environmental change through regulating guard cell turgor mediated by membrane transporters and signaling transduction. However, the origin, evolution, and active control of stomata remain controversial topics. We first review stomatal evolution and diversity, providing fossil and phylogenetic evidence of their origins. We summarize functional evolution of guard cell membrane transporters in the context of climate changes and environmental stresses. Our analyses show that the core signaling elements of stomatal movement are more ancient than stomata, while genes involved in stomatal development co-evolved de novo with the earliest stomata. These results suggest that novel stomatal development-specific genes were acquired during plant evolution, whereas genes regulating stomatal movement, especially cell signaling pathways, were inherited ancestrally and co-opted by dynamic functional differentiation. These two processes reflect the different adaptation strategies during land plant evolution.

Fossil evidence for the ancient link between clonal fragmentation, six-fold symmetry and an epizoic lifestyle in asterozoan echinoderms.

Asexual reproduction by means of splitting, also called fissiparity, is a common feature in some asterozoan groups, especially in ophiactid brittle stars. Most fissiparous brittle stars show six instead of the usual five rays, live as epibionts on host organisms, and use clonal fragmentation to rapidly colonize secluded habitats and effectively expand the margins of their distribution area. While the biology and ecology of clonal fragmentation are comparatively well understood, virtually nothing is known about the evolution and geological history of that phenomenon. Here, we describe an exceptional fossil of an articulated six-armed brittle star from the Late Jurassic of Germany, showing one body half in the process of regeneration, and assign it to the new species Ophiactis hex sp. nov. Phylogenetic inference shows that the fossil represents the oldest member of the extant family Ophiactidae. Because the Ophiactis hex specimen shows an original six-fold symmetry combined with a morphology typically found in epizoic ophiuroids, in line with recent fissiparous ophiactid relatives, we assume that the regenerating body half is an indication for fissiparity. Ophiactis hex thus shows that fissiparity was established as a means of asexual reproduction in asterozoan echinoderms by the Late Jurassic.

Earth's earliest forest: fossilized trees and vegetation-induced sedimentary structures from the Middle Devonian (Eifelian) Hangman Sandstone Formation, Somerset and Devon, SW England

The evolution of trees and forests through the Devonian Period fundamentally changed the Earth's land biosphere, as well as impacting physical environments and geomorphology by stabilizing sediments and interacting with flowing air and water. From the mid-Givetian Age onwards, lignophyte flora are known to have been key parts of the machinery in the so-called Devonian landscape factory, but the impact of earlier forests, dominated by less woody cladoxylopsids, are not as well understood. We report here evidence for a previously unrecognized cladoxylopsid forest landscape, archived within the Eifelian Hangman Sandstone Formation of Somerset and Devon, SW England. This unit has previously been considered palaeobotanically depauperate, but is here shown to contain the earliest fossil evidence for such trees in the British record, as well as the oldest known evidence globally for the relative position of standing trees: in common parlance, a fossil forest. In addition to abundant fossil material attributable to the cladoxylopsid tree Calamophyton , and other early Mid-Devonian flora, the sedimentary context of the plant remains sheds light on the biogeomorphic impacts of these earliest forests. The trees colonized a sizeable distributive fluvial system that was prone to seasonal disturbance events. The nature of the sedimentary system has created a bias to those facies where biogeomorphic signatures are most frequently recorded (from the distal parts of the system), but across the whole system there is evidence of plant–sediment interactions in the form of vegetation-induced sedimentary structures, rooting features and accumulations of plant debris. Plant remains are also found in nearshore facies adjacent to the distributive fluvial system, attesting to the development of a novel non-marine/marine teleconnection from the production and export of new biological sedimentary particles. The Hangman Sandstone Formation is illustrative of the revolutionary power of cladoxylopsid trees as biogeomorphic agents, forming densely spaced forests and shedding exceptionally abundant plant debris, while also impacting local landforms and sediment accumulations and profoundly changing landform resilience against flood disturbance events. These findings provide evidence that the Eifelian Stage (393.3–387.7 Ma) marks the onset of tree-driven changes to physical environments that would forever change Earth's non-marine landscapes and biosphere. Supplementary material: Details of sedimentary facies and additional images of plant fossils are available at https://doi.org/10.6084/m9.figshare.c.7084873

Exploring the preservation of a parasitic trace in decapod crustaceans using finite elements analysis.

The fossil record of parasitism is poorly understood, due largely to the scarcity of strong fossil evidence of parasites. Understanding the preservation potential for fossil parasitic evidence is critical to contextualizing the fossil record of parasitism. Here, we present the first use of X-ray computed tomography (CT) scanning and finite elements analysis (FEA) to analyze the impact of a parasite-induced fossil trace on host preservation. Four fossil and three modern decapod crustacean specimens with branchial swellings attributed to an epicaridean isopod parasite were CT scanned and examined with FEA to assess differences in the magnitude and distribution of stress between normal and swollen branchial chambers. The results of the FEA show highly localized stress peaks in reaction to point forces, with higher peak stress on the swollen branchial chamber for nearly all specimens and different forces applied, suggesting a possible shape-related decrease in the preservation potential of these parasitic swellings. Broader application of these methods as well as advances in the application of 3D data analysis in paleontology are critical to understanding the fossil record of parasitism and other poorly represented fossil groups.

Plastid phylogenomics and fossil evidence provide new insights into the evolutionary complexity of the ‘woody clade’ in Saxifragales

BackgroundThe “woody clade” in Saxifragales (WCS), encompassing four woody families (Altingiaceae, Cercidiphyllaceae, Daphniphyllaceae, and Hamamelidaceae), is a phylogenetically recalcitrant node in the angiosperm tree of life, as the interfamilial relationships of the WCS remain contentious. Based on a comprehensive sampling of WCS genera, this study aims to recover a robust maternal backbone phylogeny of the WCS by analyzing plastid genome (plastome) sequence data using Bayesian inference (BI), maximum likelihood (ML), and maximum parsimony (MP) methods, and to explore the possible causes of the phylogenetic recalcitrance with respect to deep relationships within the WCS, in combination with molecular and fossil evidence.ResultsAlthough the four WCS families were identically resolved as monophyletic, the MP analysis recovered different tree topologies for the relationships among Altingiaceae, Cercidiphyllaceae, and Daphniphyllaceae from the ML and BI phylogenies. The fossil-calibrated plastome phylogeny showed that the WCS underwent a rapid divergence of crown groups in the early Cretaceous (between 104.79 and 100.23 Ma), leading to the origin of the stem lineage ancestors of Altingiaceae, Cercidiphyllaceae, Daphniphyllaceae, and Hamamelidaceae within a very short time span (∼4.56 Ma). Compared with the tree topology recovered in a previous study based on nuclear genome data, cytonuclear discordance regarding the interfamilial relationships of the WCS was detected.ConclusionsMolecular and fossil evidence imply that the early divergence of the WCS might have experienced radiative diversification of crown groups, extensive extinctions at the genus and species levels around the Cretaceous/Paleocene boundary, and ancient hybridization. Such evolutionarily complex events may introduce biases in topological estimations within the WCS due to incomplete lineage sorting, cytonuclear discordance, and long-branch attraction, potentially impacting the accurate reconstruction of deep relationships.

New occurrences of the bone-eating worm Osedax from Late Cretaceous marine reptiles and implications for its biogeography and diversification.

The bone-eating worm Osedax is a speciose and globally distributed clade, primarily found on whale carcasses in marine environments. The earliest fossil evidence for Osedax borings was previously described in plesiosaur and sea turtle bones from the mid-Cretaceous of the United Kingdom, representing the only unequivocal pre-Oligocene occurrences. Confirming through CT scanning, we present new evidence of Osedax borings in three plesiosaur specimens and, for the first time, identify borings in two mosasaur specimens. All specimens are from the Late Cretaceous: one from the Cenomanian of the United Kingdom, two from the Campanian of the southeastern United States, and two from the Maastrichtian of Belgium. This extends the geographic range of Osedax in the Cretaceous to both sides of the northern Atlantic Ocean. The bones contain five borehole morphotypes, potentially created by different species of Osedax, with the Cenomanian specimen containing three morphotypes within a single tooth. This combined evidence of heightened species diversity by the Cenomanian and broad geographic range by the Campanian potentially indicates an earlier origin and diversification for this clade than previously hypothesized. Preservational biases indicate that Osedax was probably even more widely distributed and speciose in the Cretaceous than apparent in the fossil record.