Evidence-based practice is vital for the provision of high-quality and safe healthcare. However, a rigorous, standardised, and unified evidence-based practice measure that could be applied to Arabic-speaking undergraduate students did not exist prior to this study. Moreover, translating questionnaires requires considerable time and money.Thus, this study aimed to investigate the psychometric properties of the Student Evidence-Based Practice Questionnaire (English version) among Arabic-speaking undergraduate students at health sciences colleges in Saudi Arabia.A cross-sectional survey was adopted.The survey was conducted online over a six-month period.A convenience sample of 395 undergraduate nursing students was recruited from health sciences colleges at seven universities in Saudi Arabia.The students were requested to complete the survey, including the Student Evidence-Based Practice Questionnaire, as well as a background and academic data sheet. Construct validity, discriminant sensitivity, and internal reliability were evaluated.An exploratory factor analysis was performed. The Kaiser-Meyer-Olkin test value was 0.901, which was higher than the acceptable value of 0.70, indicating the adequacy of the sample size. Bartlett's test of sphericity was significant (chi-square (df: 210) = 4768.818, p < .001), indicating significant correlations within the variables and matrix. Based on Kaiser's criterion with eigenvalues > 1, four factors were detected that explained 62.03 % of the variance. The exploratory factor analysis of the 21 items generated factor loadings from 0.64 to 0.87. The Student Evidence-Based Practice Questionnaire has discriminant validity. The Cronbach's alpha and split-half reliability were over 0.7, indicating that the questionnaire had good internal consistency (Cronbach's alpha = 0.922). No evidence for floor or ceiling effects was observed.The English version of the Student Evidence-Based Practice Questionnaire has a satisfactory validity and reliability level and can be used among Arabic-speaking undergraduate students without the need to translate it into Arabic.