Evaporative Water Loss Research Articles

A proposal to account for the stimulus, the mechanism, and the mediators released in exercise-induced bronchoconstriction.

Exercise induced bronchoconstriction (EIB) describes the transient narrowing of the airways that follows vigorous exercise. It commonly occurs in children and adults who have asthma and in elite athletes. The primary stimulus is proposed to be loss of water, by evaporation, from the airway surface due to conditioning inspired air. The mechanism, whereby this evaporative loss of water provokes contraction of the bronchial smooth muscle, is thought to be an increase in osmolarity of the airway surface liquid. The increase in osmolarity causes mast cells to release histamines, prostaglandins, and leukotrienes. It is these mediators that contract smooth muscle causing the airways to narrow.

Thermal acclimatisation to heatwave conditions is rapid but sex-specific in wild zebra finches

Under climate change, increasing air temperature average and variability pose substantial thermal challenges to animals. While plasticity in thermoregulatory traits could potentially attenuate this impact, whether thermal acclimatisation can occur quickly enough to track weather variability in hot climates is unknown in any endotherm, and sex differences have never been tested. We investigated acclimatisation responsiveness of male and female wild zebra finches to short-term (< 2 weeks) summer temperature fluctuations in the Australian desert. Hotter weather before respirometry trials triggered a typical acclimatisation response (especially at chamber temperature Tchamb ≥ 40). However, acclimatisation occurred remarkably rapidly: metabolic rate responded within just one day, while body temperature (Tb) and evaporative cooling capacity (EHL/MHP) were best predicted by weather on the trial day; whereas evaporative water loss responded more slowly (1 week). Nonetheless, rapid acclimatisation only occurred in males, and females had higher Tb and lower EHL/MHP than males, potentially increasing hyperthermia risk. Furthermore, acclimatisation did not translate into greater acute heat tolerance (i.e. ability to tolerate Tchamb = 46 °C). Our results therefore reveal surprisingly rapid acclimatisation and even anticipatory adjustments to heat. However, with no changes in acute heat tolerance, and in females, phenotypic flexibility may provide only limited buffering against the detrimental impact of heatwaves.

Effect of varying sand percentage in sheath of nature-based capillary barriers composite on water storage capacity and okra growth in arid climate

Agricultural development in many arid countries including Oman is limited due to water scarcity which is currently exacerbated with increasing demand due to rapid population growth, economic development, and mismanagement of water resources. Impact of climate change is expected to adversely impact the water resources. Therefore, finding new efficient, environmentally friendly, and affordable water-saving techniques in agronomy is a necessity for achieving agricultural sustainability. Irrigation water can be saved by creating capillary barriers (CB) to unsaturated moisture flow. In this study, a “nature-inspired capillary barrier design” called hereafter as Smart Capillary Barrier (SCB) (mosaic of fine textured blocks, made of silt loam, and sandy sheath surrounding the blocks) was tested in series of field experiments with soil columns and pots. We investigated the effect of the percentage of sand of the sheath on water storage capacity of the SCB blocks and growth of okra (Abelmoschus esculentus L.) under deficit irrigation. Three proportions of sand to silt loam (sand%:silt loam%) in the sheaths were used in this study, SCB-25 (25:75%), SCB-50 (50:50%) and SCB-75 (75:25%). Our results show that increasing the percentage of sand in the designed and constructed SCB composite reduces evaporative and drainage water losses and therefore increases the water storage capacity of the blocks. Our SCB-75 attains 1.7–1.3 smaller drying rate than that for SCB-25 and SCB-50, respectively. Therefore, this composite had higher water saving capacity than a homogeneous soil (no capillary barrier) and a soil with a standard sand mulch (coarse soil overlies fine soil). The study found that using SCB composite did not consistently have a clear effect on the growth of okra plants at all stages of their development. However, during the development stage, using SCB-75 resulted in significantly higher dry biomass of the plants compared to using a homogeneous soil (the control). Additionally, during the initial stage of fruit yield, using SCB-75 also resulted in significantly higher fruit yield compared to using a homogeneous soil. Application of our SCB composite is a promising approach for saving water in desertic farming.

Hydration and evaporative water loss of lizards change in response to temperature and humidity acclimation.

Testing acclimation plasticity informs our understanding of organismal physiology and applies to conservation management amidst our rapidly changing climate. While there is a wealth of research on the plasticity of thermal and hydric physiology in response to temperature acclimation, there is a comparative gap for research on acclimation to different hydric regimes, as well as the interaction between water and temperature. We sought to fill this gap by acclimating Western Fence Lizards (Sceloporus occidentalis) to experimental climate conditions (crossed design of Hot or Cool, Dry or Humid) for eight days, and measuring cutaneous evaporative water loss (CEWL), plasma osmolality, hematocrit, and body mass before and after acclimation. CEWL changed plastically in response to the different climates, with lizards acclimated to Hot Humid conditions experiencing the greatest increase in CEWL. Change in CEWL among individuals was negatively related to treatment vapor pressure deficit and positively related to treatment water vapor pressure. Plasma osmolality, hematocrit, and body mass all showed greater changes in response to temperature than to humidity or vapor pressure deficit. CEWL and plasma osmolality were positively related across treatment groups before acclimation and within treatment groups after acclimation, but the two variables showed different responses to acclimation, suggesting that they are interrelated but governed by different mechanisms. This study is among few that assess more than one metric of hydric physiology and that test the interactive effects of temperature and humidity. Such measurements will be essential for predictive models of activity and survival for animals under climate change.

Muted responses to heat in a nocturnal tropical rodent

AbstractWe measured the thermoregulatory characteristics of a common, tropical murid rodent, Sundamys muelleri. Subcutaneous body temperatures and evaporative water loss increased at ambient temperatures above 33°C, but no increases in metabolic rate were observed as high as 38.2°C, indicating tropical rodents may be resilient as climate change progresses.Abstract in Malay is available with online material.

Thermoregulatory strategies of songbird nestlings reveal limited capacity for cooling and high risk of dehydration

How the accelerating pace of global warming will affect animal populations depends on the effects of increasing temperature across the life cycle. Developing young are sensitive to environmental challenges, often with life-long consequences, but the risks of climate warming during this period are insufficiently understood. This may be due to limited insight into physiological sensitivity and the temperatures that represent a thermal challenge for young. Here we examined the physiological and behavioural effects of increasing temperatures by measuring metabolic rate, water loss, and heat dissipation behaviours between 25–45 °C in nestlings of a small free-living songbird of temperate SE-Australia, the superb fairy-wren. We found a high and relatively narrow thermoneutral zone from 33.1 to 42.3 °C, with metabolic rate increasing and all nestlings panting above this range. Evaporative water loss sharply increased above 33.5 °C; at the same temperature, nestlings changed their posture (extended their wings) to facilitate passive heat loss. However, at all temperatures measured, water loss was insufficient to dissipate metabolically produced heat, indicating poor cooling capabilities, which persisted even when individuals were panting. While nestlings are relatively tolerant to higher temperatures, with no evidence for hyperthermia at temperatures below 42 °C, they are at a high risk of dehydration even at lower temperatures, with limited ability to mitigate this. Thus, climate warming is likely to elevate the risk dehydration, which is concerning, since it is accompanied by drier conditions.

Ecophysiological trait variation in desert versus Mediterranean populations of a gecko

AbstractPopulations inhabiting several biomes may experience different abiotic and biotic conditions, exerting local selection pressures. Temperature and water regimes are interconnected variables, that may differ between biomes, and greatly influence ecophysiological traits, such as metabolic and evaporative water loss rates. We hypothesized that Ptyodactylus guttatus (Sinai Fan‐fingered Gecko) individuals, which occupy the Mediterranean and desert biomes across Israel, would follow the “metabolic cold adaptation” hypothesis and be adapted to the microclimate in the biome they inhabit. We thus predicted that desert individuals would prefer lower temperatures, and have lower resting metabolic rates and evaporative water loss rates at higher ambient temperatures than Mediterranean individuals. We also predicted that Mediterranean individuals would have a better body condition than individuals from the desert, because of higher primary productivity in the Mediterranean biome, and would therefore have higher mite loads. We further predicted that geckos from the desert would have longer limbs, enabling them to lose more heat to the environment, according to Allen's rule. To test these hypotheses, we measured the temperature preferences, field body temperatures, resting metabolic rates, evaporative water loss rates, body conditions, mite loads, and limb lengths of 82 P. guttatus individuals collected from four localities two from the desert biome and two from the Mediterranean biome. There were no significant differences in any of the tested traits when comparing between biomes. However, we found some differences in the evaporative water loss rates, body temperatures, body condition, and forelimb lengths between the northernmost and southernmost, and driest and wettest localities, supporting some of our predictions. Our results highlight the importance of the resolution of the analysis. Although some ecophysiological traits of P. guttatus seem to be conserved across localities and biomes, thermal plasticity in these traits may have helped this species reach its current distribution and occupy two biomes.

Functional responses in a lizard along a 3.5‐km altitudinal gradient

AbstractAimPhysiological and metabolic performance are key mediators of the functional response of species to environmental change. Few environments offer such a multifaceted array of stressors as high‐altitude habitats, which differ markedly in temperature, water availability, UV radiation and oxygen pressure compared to low‐altitude habitats. Species that inhabit large altitudinal gradients are thus excellent models to study how organisms respond to environmental variation.LocationTenerife island, Canary Islands archipelago (Spain).TaxonTenerife lizard (Gallotia galloti, Lacertidae).MethodsWe integrated data on age structure, thermal and hydric regulatory behaviour and four metabolic and stress‐related biomarkers for an insular lizard that inhabits an extreme altitudinal range (sea level to 3700 m a.s.l.), to understand how an ectotherms' age, ecophysiology and metabolism can be affected by extreme environmental variation.ResultsWe found marked differences in metabolic stress markers associated with altitude (particularly in the abundance of carbonyl metabolites and relative telomere length), but without a linear pattern along the altitudinal cline. Contrary to expectations, longer telomeres and lower carbonyl content were detected at the highest altitude, suggesting reduced stress in these populations. Evaporative water loss differed between populations but did not follow a linear altitudinal gradient. Lizard age structure or thermal physiological performance did not markedly change across different altitudes. Mixed signals in life‐history and thermal ecology across populations and altitude suggest complex responses to variable conditions across altitude in this species.Main ConclusionsOur integrative study of multiple functional traits demonstrated that adaptation to highly divergent environmental conditions in this lizard is potentially linked to an interplay between plasticity and local adaptation variably associated with different functional traits.

Beauty or function? The opposing effects of natural and sexual selection on cuticular hydrocarbons in male black field crickets.

Although many theoretical models of male sexual trait evolution assume that sexual selection is countered by natural selection, direct empirical tests of this assumption are relatively uncommon. Cuticular hydrocarbons (CHCs) are known to play an important role not only in restricting evaporative water loss but also in sexual signalling in most terrestrial arthropods. Insects adjusting their CHC layer for optimal desiccation resistance is often thought to come at the expense of successful sexual attraction, suggesting that natural and sexual selection are in opposition for this trait. In this study, we sampled the CHCs of male black field crickets (Teleogryllus commodus) using solid-phase microextraction and then either measured their evaporative water loss or mating success. We then used multivariate selection analysis to quantify the strength and form of natural and sexual selection targeting male CHCs. Both natural and sexual selection imposed significant linear and stabilizing selection on male CHCs, although for very different combinations. Natural selection largely favoured an increase in the total abundance of CHCs, especially those with a longer chain length. In contrast, mating success peaked at a lower total abundance of CHCs and declined as CHC abundance increased. However, mating success did improve with an increase in a number of specific CHC components that also increased evaporative water loss. Importantly, this resulted in the combination of male CHCs favoured by natural selection and sexual selection being strongly opposing. Our findings suggest that the balance between natural and sexual selection is likely to play an important role in the evolution of male CHCs in T. commodus and may help explain why CHCs are so divergent across populations and species.

Stable isotope tracing internal recycling and evaporation losses in saline lakes on the Qinghai-Tibet Plateau

Direct measuring of internal lake recycling and evaporation losses remains challenging for lakes on the Qinghai-Tibet Plateau (QTP). Stable isotope techniques provide an effective approach for estimating water vapor cycling ratios and evaporation losses of lakes on the QTP. In this study, the stable isotope values of saline lakes on the QTP were modeled using the stable isotope values of the sampled lake water and their influencing factors. The water vapor recycling ratio and evaporation loss (E/I) of 135 saline lakes on the QTP were evaluated and their influencing factors were revealed. The results showed that stable isotopes in saline lakes on the QTP showed significant spatial variability. Their stable isotopes were affected by the source of water vapor, recharge patterns, and local evaporation conditions. It's worth noting that the average water vapor recycling ratio of saline lakes on the QTP was 20.16 %, one-fifth of the saline lakes had a water vapor recycling ratio beyond 30 %. Saline lakes lose 26 % of their water through evaporation. 26 % of the saline lakes experienced high evaporation losses of >40 % of the total inflow. We found that the main factors controlling the water vapor recycling ratio and evaporation loss in saline lakes on the QTP were precipitation and altitude, respectively. Interestingly, the control factors of water vapor recycling ratio and evaporation loss in saline lakes with elevation above 4500 m showed significant differences compared to saline lakes with elevation below 4500 m. Therefore, the strengthening of lake system monitoring can provide reliable data support for security assessment and effective management of water resources on the QTP.

Soil management considerations for water resiliency in a changing climate

AbstractProviding adequate water for crops is a key consideration for agricultural resiliency. Climate change is expected to exert substantial pressure on crop water supplies through increased rainfall variability and increased evaporative demand. The temporal and spatial variability of these climate change impacts, in combination with many feedbacks, at times operating at different scales, makes identifying specific, yet broadly applicable “one‐size‐fits‐all” soil management solutions challenging. Adaptations that work well in one location may represent a poor management choice in another. Considering the local combinations of climate, soils, and crop production systems is fundamental for identifying appropriate strategies. Nonetheless, some broadly applicable soil management pathways for increasing soil water availability can be identified: (i) maximizing rainfall capture, (ii) maximizing soil water storage capacity (intensity × volume), and (iii) suppressing unproductive evaporative water losses, particularly during intervals when leaf area of the primary crop is low. Selecting climate, soil, and production‐system‐appropriate tillage and surface cover management practices is among the most relevant considerations for improving soil water availability. Maintaining surface cover favors evaporation suppression and increased capture of precipitation. Tillage choices affect internal soil water storage through impacts on soil properties that influence storage intensity and by altering the volume of soil available for root growth, as well as through effects on surface cover. Promoted management practices aimed at improving soil water availability for increased agricultural resiliency must be practically feasible and cost‐effective in order to support their broad adoption, and such constraints should be an important consideration in their development.

Rapid Physiological Plasticity in Response to Cold Acclimation for Nonnative Italian Wall Lizards (Podarcis siculus) from New York.

AbstractThermal physiology helps us understand how ectotherms respond to novel environments and how they persist when introduced to new locations. Researchers generally measure thermal physiology traits immediately after animal collection or after a short acclimation period. Because many of these traits are plastic, the conclusions drawn from such research can vary depending on the duration of the acclimation period. In this study, we measured the rate of change and extent to which cold tolerance (critical thermal minimum [CTmin]) of nonnative Italian wall lizards (Podarcis siculus) from Hempstead, New York, changed during a cold acclimation treatment. We also examined how cold acclimation affected heat tolerance (critical thermal maximum [CTmax]), thermal preference (Tpref), evaporative water loss (EWL), resting metabolic rate (RMR), and respiratory exchange ratio (RER). We predicted that CTmin, CTmax, and Tpref would decrease with cold acclimation but that EWL and RMR would increase with cold acclimation. We found that CTmin decreased within 2 wk and that it remained low during the cold acclimation treatment; we suspect that this cold tolerance plasticity reduces risk of exposure to lethal temperatures during winter for lizards that have not yet found suitable refugia. CTmax and Tpref also decreased after cold acclimation, while EWL, RMR, and RER increased after cold acclimation, suggesting trade-offs with cold acclimation in the form of decreased heat tolerance and increased energy demands. Taken together, our findings suggest that cold tolerance plasticity aids the persistence of an established population of invasive lizards. More generally, our findings highlight the importance of accounting for the plasticity of physiological traits when investigating how invasive species respond to novel environments.

Construction and testing of a low-cost device for the collection of rainfall samples destined for stable isotope analysis

Oxygen- and hydrogen-isotope ratios in rainfall provide important hydroclimatic information, yet despite a global network of rainfall isotope measurements, significant geographical gaps exist in data coverage, with only three long-term stations spanning the southern African region. Project-based, ad hoc collections of rainfall for isotope analysis can improve this coverage. However, all rainfall samples that are destined for stable isotope analysis must be collected in such a way to avoid evaporation and resultant isotope fractionation. While such rainwater collectors are available commercially, both the product and shipping are prohibitively costly. We describe the construction of a simple rainfall collector using a design from the literature and materials that are readily available in South African hardware stores. Our rainwater collector can be constructed for the much lower cost of just under ZAR820 in comparison with the cost of ZAR9300 inclusive of shipping from commercial outlets (2022 prices). Our design modifications have the added advantage of portability, with the rainwater collector housed in a bucket with a handle. The device was tested by comparing its performance, in terms of evaporative water loss and isotopic fractionation, with that of an open bottle, using tap water in both cases. Testing confirmed that the collector prevented evaporation over a one-week period, indicating that it is suitable for weekly or more frequent sampling of rainfall. Although the design described was based on materials procured in South Africa, it could easily be adapted for construction elsewhere. Significance: Hydrogen and oxygen isotope composition of rainfall provides valuable climatic information. Rainwater collectors for stable isotope samples must prevent evaporation, as evaporation will alter the isotopic signature. We describe the construction and testing of a bespoke, low-cost and portable device that can be used to collect rainfall samples destined for oxygen- and hydrogen-isotope analysis without significant evaporation.

An evaluation of a biophysical model for predicting avian thermoregulation in the heat.

Survival and reproduction of endotherms depend on their ability to balance energy and water exchange with their environment, avoiding lethal deficits and maximising gains for growth and reproduction. At high environmental temperatures, diurnal endotherms maintain body temperature (Tb) below lethal limits via physiological and behavioural adjustments. Accurate models of these processes are crucial for predicting effects of climate variability on avifauna. We evaluated the performance of a biophysical model (NicheMapR) for predicting evaporative water loss (EWL), resting metabolic rate (RMR) and Tb at environmental temperatures approaching or exceeding normothermic Tb for three arid-zone birds: southern yellow-billed hornbill (Tockus leucomelas), southern pied babbler (Turdoides bicolor) and southern fiscal (Lanius collaris). We simulated metabolic chamber conditions and compared model outputs with thermal physiology data collected at air temperatures (Tair) between 10 and 50°C. Additionally, we determined the minimum data needed to accurately model diurnal birds' thermoregulatory responses to Tair using sensitivity analyses. Predicted EWL, metabolic rate and Tb corresponded tightly with observed values across Tair, with only minor discrepancies for EWL in two species at Tair≈35°C. Importantly, the model captured responses at Tair=30-40°C, a range spanning threshold values for sublethal fitness costs associated with sustained hot weather in arid-zone birds. Our findings confirm how taxon-specific parameters together with biologically relevant morphological data can accurately model avian thermoregulatory responses to heat. Biophysical models can be used as a non-invasive way to predict species' sensitivity to climate, accounting for organismal (e.g. physiology) and environmental factors (e.g. microclimates).

Incorporating species-specific morphology improves model predictions of thermal and hydric stress in the sand fiddler crab, Leptuca pugilator

Understanding where and why organisms are experiencing thermal and hydric stress is critical for predicting species' responses to climate change. Biophysical models that explicitly link organismal functional traits like morphology, physiology, and behavior to environmental conditions can provide valuable insight into determinants of thermal and hydric stress. Here we use a combination of direct measurements, 3D modeling, and computational fluid dynamics to develop a detailed biophysical model of the sand fiddler crab, Leptuca pugilator. We compare the detailed model's performance to a model using a simpler ellipsoidal approximation of a crab. The detailed model predicted crab body temperatures within 1 °C of observed in both laboratory and field settings; the ellipsoidal approximation model predicted body temperatures within 2 °C of observed body temperatures. Model predictions are meaningfully improved through efforts to incorporate species-specific morphological properties rather than relying on simple geometric approximations. Experimental evaporative water loss (EWL) measurements indicate that L. pugilator can modify its permeability to EWL as a function of vapor density gradients, providing novel insight into physiological thermoregulation in the species. Body temperature and EWL predictions made over the course of a year at a single site demonstrate how such biophysical models can be used to explore mechanistic drivers and spatiotemporal patterns of thermal and hydric stress, providing insight into current and future distributions in the face of climate change.

Synergistic optimization of partition water distribution, non-equidistant fillings and dry-wet hybrid rain zone for wet cooling towers

Existing single-zone or two-zone synergistic optimization technologies have limited performance improvements for the large natural draft wet cooling towers (LNDWCTs). To further improve the cooling performance of the LNDWCTs, this study proposes for the first time a three-zone (water-spraying, fillings and rain zones) synergistic optimization method from a global optimization perspective. The 3D numerical calculation model is created and validated. Then, this work investigates emphatically the impact of the different three-zone parameters on the cooling performance, including the partition water distribution radius (R1 = 20 ∼ 60 m), the water distribution percentage in the inner zone (γ=5 ∼ 90 %), the fillings partition radius (R2 = 0 ∼ 65 m), the total coverage area of the split-flow plate (S = 2000 ∼ 2900 m2), the installation angle (θ=0 ∼ 27°), the split-flow plate length (L = 15 ∼ 55 m) and number (n = 3 ∼ 10). The simulation results illustrate that, within the studied range, the water temperature drop, cooling efficiency and Merkel number increase and then decrease with the increasing R1,γ, R2, θ and n, and increase successively with the increasing S and L. The optimized three-zone parameter combination scheme is R1 = 55 m, γ=75 %, R2 = 50 m, S = 2900 m2, θ=6°, L = 55 m and n = 7. Compared to the LNDWCT without any optimization technology, the water temperature drop, cooling efficiency, Merkel number, ventilation and evaporation loss of the cooling tower with optimized three-zone synergistic pattern increase by 0.58 °C, 3.37 %, 0.17, 1480 kg/s and 41.28 kg/s, respectively. This study can lay a theoretical basis for the multi-zone synergistic optimization research of LNDWCTs and provide a reference for the technical modifications and engineering applications.

Do urban habitats induce physiological changes in Mediterranean lizards?

AbstractUrban environments offer dramatically different habitats for wildlife compared with natural environments. They provide, for example, different levels of resource availability, anthropogenic night light, and microclimates (e.g., urban heat islands). For reptiles, increased temperatures in the city can lead to increased energetic demands and metabolic rates unless they change their morphology and physiology and adapt or acclimatize to the urban conditions. We explored differences in metabolic rate, evaporative water loss, and body size of two lizard species – rock agamas (Laudakia vulgaris) and Mediterranean house geckos (Hemidactylus turcicus), from urban habitats and nearby natural open areas. When tested in the lab, we expected to identify physiological adaptations resulting in decreased metabolism in urban individuals. Both species had similar body lengths and masses at both habitat types, suggesting any differences in costs and benefits between urban and natural environments do not affect their overall size or body condition. In the laboratory, metabolic rates were similar in individuals from both habitats for both species, indicating no long‐term adaptations in this trait. However, urban geckos (but not agamas) had higher evaporative water loss than conspecifics from more natural habitats. This may suggest different compositions of epidermal lipids affecting the gecko skin's resistance to evaporation between the habitats. Overall, our results highlight different elements of the urban environment that might affect reptiles. However, the differences between species urge caution in interpreting the results to other species and locations. With increasing urbanization worldwide, understanding when and to what degree local adaptations can occur can help us predict reptile species distribution and survival in light of future anthropogenic changes.

Metabolic rate and evaporative water loss during a shedding event in the Mediterranean House Gecko Hemidactylus turcicus

Metabolic rate and evaporative water loss during a shedding event in the Mediterranean House Gecko Hemidactylus turcicus

Latitudinal variation in the functional response of Quercus suber seedlings to extreme drought

Many plant species are being threatened by increasingly drought conditions due to current climate change at planetary scale. This global trend is leading to the scientific community to investigate the potential role of local adaptations through intraspecific differences in functional traits that may boost conservation strategies by modulating the plant responses to reduced water availability. We assessed under controlled conditions the effect of four different drought intensities on the survival time and morphological traits of Quercus suber seedlings collected from nine populations covering the complete latitudinal distribution of the species. Functional morphological traits related to biomass allocation and leaf and root display were analyzed. We then related these traits with the survival time after a terminal desiccation, used as a drought-resistance proxy and expressed as survival time without watering. Abundant watering availability allowed seedlings to survive for a longer period compared to drier conditions. Further, all morphological traits differed across watering levels, showing a very plastic response. Acorns from southern latitudes produced very large seedlings compared to those gathered from northern latitudes. However, the larger biomass implied higher evaporative water loss, inducing lower survival of southern populations under extreme drought conditions. We further found a clear trend toward maximizing those traits related with belowground growth (i.e., root surface area, root average diameter and root volume) in southern populations aimed to increase water uptake, overcoming the most limiting factor for plant growth in that area. Our results support that increased root development allow cork oak to maintain its functioning after being subjected to damage caused by reduced water availability, whereas high aerial biomass allocation is a handicap for survival under drought stress conditions. This study identifies drought-resistant populations and morphological traits related to drought resistance, which can be applied to improve restoration actions under a warmer climate.

Using hydrogen and oxygen stable isotopes to estimate soil water evaporation loss under continuous evaporation conditions

AbstractThe use of hydrogen and oxygen stable isotopes in estimating soil water evaporation loss under continuous evaporation conditions is crucial for gaining insight into soil water movement processes under different conditions. In this study, via high‐frequency meteorological monitoring and continuous soil water measurements, we investigated the variation of hydrogen and oxygen stable isotopes and soil water fluxes with soil depth and time for soil water at different depths under continuous evaporation conditions. The precipitation isotope δrain and soil water flux changes were determined using the Craig‐Gordon model. It was shown that the isotopic fractionation of soil water in the surface layer 0–30 cm was dominated by a gaseous‐dominated transport process, and that both the δ18O and δD values and evaporative intensity decreased with increasing soil depth. In terms of time dynamics, the evaporation loss of soil water varies continuously with seasons and is the highest during summer. The use of δ18O to quantify the soil water evaporation loss provides greater accuracy than that provided by δD. The relative errors in the evaporation loss calculated based on δ18O and δD were 13% and 34%, respectively. A sensitivity analysis of each parameter indicated that the relative error calculated by the model is primarily determined by temperature and relative humidity uncertainty. The sensitivity analysis reveals the critical evaporation intensity of soil water at various depths from unsteady to steady state evaporation. When the relative humidity changes by 1%, the evaporation loss fraction changes from 0.001 to 0.034. The results of this study are important for quantifying the soil water resources in arid and semi‐arid areas without precipitation using stable isotopes of hydrogen and oxygen.