Serum Lipid Profile In Rats Research Articles

Evaluation of Serum Lipid Profile and Renal Function Levels in Iraqi Patients with Chronic Kidney Disease (CKD)

Chronic Kidney Disease (CKD) has been raised as a public health state worldwide and is associated with increased cardiovascular morbidity and mortality. The aim of the study was to assess serum lipid profile levels, renal function and glucose in CKD patients undergoing hemodialysis. A total of 50 patients (23 males and 27 females) with CKD attending the hemodialysis at Kidney Unit in Al-Ramadi Teaching Hospital, Anbar Iraq and of ages ranging between 20 -85 years, were sustained for this research. The patients were classified into three categories with their mean age of 65.71±1.98 years. Eleven healthy adults were also recruited in this study with mean age of 37.00±15.56 years as control group. The study included measuring biochemical markers such as glucose, total cholesterol(TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very-low-density lipoprotein (VLDL) , creatinine and urea by using automated chemistry analyzer by Abbott/USA. The outcomes of this study showed that the urea and creatinine levels increased significantly, whereas HDL-C level significantly decreased in the patients as compared with control group. However, TC and LDL-C showed slightly lower levels which were within the normal limits in the control group. While TG, VLDL and glucose levels showed non-significant changes in CKD patients as compared with control (P ≤ 0.05). In addition, all parameters revealed no significant changes in males as compared with females group (P ≤ 0.05). Depending on age, only creatinine level showed significant change in 20-40 years age group as compared with 61-85years age group (p <0.05). Finally, abnormal lipid metabolism may probably be an independent risk factor for the progression of renal disease.

Different Sources of Omega-3 Fatty Acid Supplementation vs. Blood Lipid Profiles—A Study on a Rat Model

Dyslipidemia is a serious condition affecting an increasing number of people, and thus, preventive measures, including supplementation, are being developed. We aimed to compare the effect of linseed oil, its ethyl esters and fish oil supplementation on the serum lipid profiles of rats fed a high-fat diet. Wistar rats were divided into nine groups. Four of them were fed a high-fat diet for the whole experiment, four groups were fed a high-fat diet before the supplementation period and then the control one with supplements, and one was fed a control diet without supplements. The whole experiment lasted 12 weeks. A significant reduction in blood triglycerides, total cholesterol and the LDL fraction was noted in supplemented groups compared to the controls, especially in groups supplemented with ethyl esters of linseed oil and linseed oil compared to fish oil groups. The results were also more beneficial in groups where, in addition to supplementation, there was also a diet change from a high-fat diet to a control diet during the supplementation period. We may conclude that supplementation with omega-3 fatty acids, combined with a healthy diet, may be a good way of preventing or alleviating dyslipidemia.

Effects of Alcoholic Extracted and Dry Eggplant (Solanum Melongena) on Hyperlipidemia Treatment in Rats

This study was carried out to investigate the potential of crude ethanolic extracts of eggplant (Solanum melongena) peels, fruit, and seeds, as well as dry eggplant, in the treatment of hyperlipidemia in rats. Forty-nine adult female Sprague Dawley rats were used with a weighting between (280-340) grams. Following the establishment of hyperlipidemia by providing them with a high-fat diet consisting of 4% cholesterol with 5% egg yolk for a period of 30 days in all groups except the control negative. Afterward, the rats were divided into seven groups of 7 rats as followed: The first group control negative was given standard pellets and water only, and the second group control positive induction hyperlipidemia this applied to all groups. Other groups (third, fourth, and five) were administered with alcoholic extracts of Solanum Melongena parts including the peel group, fruit group, and seed group, at a dose of 400 mg/kg/B.W. orally once a day, and group six received dry eggplant without extract at a dose of 200 mg/kg/diet once daily. The seven groups received atorvastatin at a dose of 20 mg/ B.W. orally once daily for a period of 45 days for all groups. The results of the study on serum lipid profile in rats indicated that the peel and atorvastatin groups exhibited a significant decrease in levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and low-density lipoprotein (VLDL). Furthermore, the peel and atorvastatin groups had substantially elevated high-density lipoprotein (HDL) levels. The study suggests that eggplant peel extract and atorvastatin, a common drug, can potentially treat hyperlipidemia in rats.

The evaluation of serum lipid profile and apolipoprotein C-1 in the Iranian patients of Oral Squamous Cell Carcinoma.

The key role of apolipoprotein C-1 (ApoC-1) is reported in breast, pancreas and lung cancer. However, no information is available on potential difference of ApoC-1 between OSCC patients and healthy individuals. This work aimed to examine the serum ApoC-1 level as well as lipid profile values between OSCC patients and healthy control groups. In this study, 44 blood samples from 22 OSCC patients and 22 healthy individuals were collected to determine the values of lipid profile and ApoC-1 concentration using colorimetric method and Enzyme-Linked Immunosorbent Assay (ELISA), respectively. A significant decrease in serum lipid profile and ApoC-1 concentration was observed between OSCC and healthy control groups (P = 0.001). Our results confirm the previous findings on the significant differences of lipid profile between OSCC and controls, also show the lower serum level of ApoC-1 in OSCC as compared to the controls. Future studies would further elaborate the association of ApoC-1 with OSCC.

Evaluation of serum lipid profile in breast cancer patients: a case control study

Objective: This study was conducted to investigate the relationship between serum lipid profile and breast cancer disease.Patients and methods: One hundred breast cancer women and 75 women with benign breast mass as a control group were included in the study. The control and the patient groups were aged matched. The age range of the control group was between 25 to 70 years (mean ± SD: 50.4 ± 15.1 years). The age range of the patient group was between 25 to 71 yeas (mean ± SD: 49.1 ± 14.6 years). The patients were undergone radical modified mastectomy, then treated with chemotherapy and radiotherapy after the operation. Five mL of blood samples from the patient and control groups were taken, and the patient samples were taken before the operation. The blood samples were analyzed for serum lipid profile. The patients and the control groups were advised to fast for 14 hours before taking the blood samples. Body mass index was measured for the patients and controls. The stage, lymph involvement, and metastasis of the cancer were also included.Results: Body mass index (BMI) in patients was significantly higher than controls. However, serum total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C) and atherogenic index (AI), in the patients were significantly lower than controls. High-density lipoprotein cholesterol (HLD-C) was higher in the patients compared with the controls but not significant.Conclusion: a significant decrease in TC, TGs, and LDL-C with no significant increase for HDL-C was noticed in breast cancer women. Serum lipid profile may not be considered as a risk factor for breast cancer.

Lipid profile of the ethanol - methanol (1:1) extracts of Anacadium occidentale and Jatropha tanjorensis administration in Wistar rats

Hyperlipidaemia is characterized by an increase in one or more of the plasma lipids. This study evaluated the lipid profile of albino Wistar rats administered ethanol-methanol extracts of Anacadium occidentale and Jatropha tanjorensis leaves. Twenty-five (25) male Wistar rats weighing between 180 - 220 g were divided into five (5) groups of five (5) rats each and treated thus: Group 1 (normal control received normal saline), group 2 and 3 administered low dose (400 mg/kg-1 b.wt.) and high dose (800 mg/kg-1 b.wt.) of Anacadium occidentale extract respectively, group 4 and 5 administered low dose (400 mg/kg-1 b.wt.) and high dose (800 mg/kg-1 b.wt.) of Jatropha tanjorensis extract respectively. At the end of the experiment, the rats were sacrificed to obtain the sera for the evaluation of serum lipid profile. The result revealed a significant (P<0.05) decrease in TC in all the groups except group IV compared with control. There was a significant (P<0.05) increase in TG and VLDL-c levels in all groups compared with control. HDL-c level significantly (P<0.05) increased in group III only compared with control. LDL-c level significantly (P<0.05) decreased in group III and V only compared with control. The LDL-c/HDL-c ratio showed no significant (P0.05) difference between groups II, V and control, however group III significantly (P<0.05) decreased and group IV significantly (P<0.05)increased compared with the rest groups. The study suggests that the plant extracts possess lipid lowering potentials and may be employed in the treatment of metabolic disorders such as obesity and cardiovascular diseases.

Evaluation of Serum Lipid Profile in Hypertensive Patients at Mirpur Khas, Sindh, Pakistan

Aim: To assess the lipid profile of hypertensive patients. Study Design: Evaluation-based study. Place and duration of study: Department of Medicine, Muhammad Medical College Hospital, Mirpurkhas, Sindh, Pakistan for a period of one year from 1stJuly 2020 to 30thJune 2021. Methodology: Three hundred and fifty-three hypertensive patients were enrolled in the study based on Rao-soft formula. Only those hypertensive patients who had confirmed diagnosis of hypertension were enrolled. The patients were requested for fasting to take the blood sample. Results: 64.87% were males and 35.1% were females. 39.6% patients were above 60 years old and highest range values for systolic blood pressure 201-220mmHg. The majority of the patients 26.34% showed 91-100 mm Hg. The 11.05% of the patients were prescribed with the Atenolol, 7.08% of patients were prescribed with another beta blocker medication Propranolol. The majority of patients were prescribed with Rosuvastatin as antilipidemic drugs however the least majority i.e. 11.61 were prescribed with Simvastatin.The 16.9% patients showed HDL40mg/dL, higher LDL<100mg/dL, total glycerides<150mg/dL, total cholesterol<200mg/dL HDL30-39mg/dL, LDL100-150mg/dL, total glycerides150-200 mg/dL, total cholesterol200-250mg/dL among 31.73% of the patients. The 28.9% of the patients showed the pattern HDL20-29mg/dL. Conclusion: The dyslipidemia associated with the hypertension and hypertensive patients. The irregular values and alteration of in the serum cholesterol and total cholesterol levels of lipid profile proves the association of lipid profile with the elevated systolic blood pressure levels. The total cholesterol, LDL and HDL can be controlled with an appropriate controlling of high blood pressures, thus preventing the cardiovascular diseases and cardiovascular diseases. Key words: Lipid, Hypertension, Assess

Evaluation of Serum Lipid Profile in Type II Diabetes Patients at Mirpur Khas, Sindh, Pakistan

Aim: To assess the serum cholesterol levels of the diabetes patients in the rural areas of the Sindh. Study design: Prospective observational study Place and duration of study: Department of Medicine, Muhammad Medical College Hospital, Mirpurkhas Sindh, Pakistan from 1st January 2020 to 31st December 2020. Methodology: Five hundred and seventeen diabetic type II patients were enrolled in the study after the written informed consent. The serum lipid profile was assessed and analyzed the relation with glycemic values and their treatment. The prescription was also assessed and data were transferred in to data analysis sheet. Results: There were 368 (71.18%) males and 149 (28.82%) females. Moreover 223(43.13%) patients were aged from 51 to 60 years. Patients had 3 to 6 years of diabetic history (51.26%). Patients had glycemic value 151-200mg/dL (33.27%). One hundred and eighty nine patients had total cholesterol value 201-250 mg/dL. One hundred and seventy six patients were prescribed from Sulphonylurea with 34.04%, 205 patients were prescribed lovastatin (39.65%), 97 patients were prescribed rosuvastatin Conclusion: The changes in the lipid profile levels of diabetic patients might occur at different ages along with the morbid condition such as diabetes mellitus and condition can be more complicated if not managed properly. The disruption in the serum lipid profile, total cholesterol, triglycerides, LDL, HDL and obesity cannot be ignored the condition is worse in developing countries like Pakistan. Keywords: Lipid, Diabetes Mellitus, Mirpurkhas, Sindh, Pakistan

Evaluation of serum lipid profile and sex hormones in women with polycystic ovary syndrome - A comparative study

Polycystic ovary syndrome (PCOS), an endocrinological disorder with lipid disturbances leads to a wide spectrum of clinical manifestations including menstrual irregularities, infertility, obesity and hyperandrogenism. This study aimed to determine the levels of lipid profile and sex hormones and its effect on PCOS from a State in southern India. This comparative hospital-based study was conducted in the State of Karnataka, India from June 2019 to January 2020. 57 age-matched PCOS and 67 healthy controls were enrolled for the study. Fasting glucose, insulin, lipid profile and sex hormone levels were analyzed after taking informed consent from all participants. The mean age of patients with PCOS was 25.05 ± 6.04 years and the mean age of subjects in the control group was 27.36 ± 7.08 years. Lipid profile showed statistically significant increased levels of triglyceride 147.3±86.6 (p<0.05) and decreased levels of HDL 52.2±8.7 (p<0.05) whereas hormones LH and testosterone were significantly higher in women with PCOS when compared to controls. The altered lipid profile, sex hormone and insulin levels exhibit a key role in the pathophysiology of PCOS that affects health. Insulin resistance is found to be linked with dyslipidemia in PCOS. Our findings suggest that the differences found may play a key role in the pathophysiology of PCOS which in turn affects the health and therefore it is advisable to emphasize the necessity for screening insulin resistance and perform early and periodic examination of lipid profile and sex hormones in women with PCOS to reduce complications.

Evaluation of Serum Lipid Profile and Malondialdehyde Levels in Obesed Adults

Evaluation of Serum Lipid Profile and Malondialdehyde Levels in Obesed Adults

Influence of purple basil (Ocimum basilicum L.) extract and essential oil on hyperlipidemia and oxidative stress in rats fed high-cholesterol diet

The aim of this study was to investigate and compare the effects of purple basil methanolic extract and essential oil on serum lipid profiles in rats fed a high cholesterol diet. Feeding a high-cholesterol diet increased serum lipid profiles, leptin, hepatic triglyceride, cholesterol levels, malondialdehyde concentrations, fatty acid synthase (FAS), liver X receptors (LXRs), ATP-citrate lyase (ACLY), and sterol regulatory element-binding proteins (SREBP-1c) expression in the liver in rats. The extract and essential oil reduced serum triglycerides by 28.2%, 30.7%, and total cholesterol by 21.7% and 14.5%, respectively. The applications did not return the aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities to their normal levels. Extract and fenofibrate administration decreased serum leptin levels by 17.5% and 27.5%, respectively. The extract reduced hepatic triglyceride by 16.4% and total cholesterol by 22.1%. Fenofibrate reduced the triglyceride and hepatic total cholesterol by 25.5% and 46.3%, respectively. The decrease in MDA levels was 34.6% in those treated with the extract, and less in those who were applied essential oil. Expression of fatty acid synthase (FAS), liver X receptors (LXRs), ATP-citrate lyase (ACLY), and sterol regulatory element-binding proteins (SREBP-1c) in the liver decreased by 20.9%, 15.4%, 20.7% and 16.9%, respectively, when the extract was administered. For those treated with essential oil, the decline was 9.9%, 13.6%, 10.2%, and 12.5%. It decreased by 38.1%, 56.8%, 21.7% and 40.8% in those administered fenofibrate. The ABTS and DPPH based antioxidant activities and total phenolic contents of the extract were 67.83%, 86.98% and 57.94 ± 0.39 mg Gallic acid equivalent (GAE), respectively. The results reveal that methanolic extract of purple basil may contain hypolipidemic and antioxidant substances and its protective effect on the liver.

Evaluation of Serum Lipid Profile at Different Trimesters among Pregnant Women in Ovia North-East Local Government Area, Edo State, Nigeria

Evaluation of Serum Lipid Profile at Different Trimesters among Pregnant Women in Ovia North-East Local Government Area, Edo State, Nigeria

Evaluation of Serum Lipid Profile after Treatment with Various Chemotherapeutic Scheduals in Iraqi Breast Cancer Patients

Evaluation of Serum Lipid Profile after Treatment with Various Chemotherapeutic Scheduals in Iraqi Breast Cancer Patients

Evaluation of Serum Lipid Profile after Different Chemotherapeutic Regimens in Iraqi Patients with Breast Cancer

Objective: To investigate the effect of doxorubicin, cyclophosphamide and taxane chemotherapy on thelipid profile in Iraqi patients with breast cancer.Method: This cohort prospective study was carried out at the Biochemistry Department, Baghdad Collegeof Medical Sciences, and at the Oncology Clinic, Oncology Teaching Hospital, Baghdad, Iraq, during theperiod from May 2019 to October 2019. It included 56 women with regular menstrual cycle (25-45 years)classified into 3 groups: GI: 29 women with primary breast cancer (before starting chemotherapy), GII:the same 29 women of GI who were finished 4 cycles of anthracycline chemotherapy (course 1) and GIII:which involved another 27 women who had finished both courses of chemotherapy, (course 1) and 4 cyclesof taxanes (course 2). Serum cholesterol, LDL, HDL and TG were measured using colorimetric method.Results: The results of the present study showed that the (mean±SEM) of the serum cholesterol wasdecreased significantly (p<0.05) in GIII compared to GI and highly significantly (p<0.01) compared to GII.While, the serum HDL level was significantly (p<0.05) lower in GIII compared to GI. Concerning the serumLDL level, it was increased significantly (p<0.05) in GII compared to both GI and GIII. While; the serumTG level shows no significant difference between all groups.Conclusion: Iraqi women with breast cancer had an abnormal state of dyslipidemia that become worsenedafter chemotherapy.

Evaluation of Serum Lipid Profile and Oxidative Stress Markers of Wistar Rats Treated with Extract of Momordica Charantia

Evaluation of Serum Lipid Profile and Oxidative Stress Markers of Wistar Rats Treated with Extract of Momordica Charantia

Evaluation of serum lipid profile and complete hemogram in patients with oral submucous fibrosis: A case control study

Aim: Not many studies in the literature are found that include all the variables under complete blood picture along with lipid profile. The aim of study was to evaluate the serum lipid profile and complete blood picture in patients with oral submucous fibrosis (OSMF). Materials and Methods: It is a case control study with the sample size of 40 patients considered by random sampling, which includes 20 clinically diagnosed patients with OSMF as per the diagnostic criteria of Passi et al. and 20 sex-matched controls. Five milliliters of blood was collected from the most prominent vein of right cubital fossa and stored in test tubes. Centrifugation was carried out for about 4–5min at 2500rpm and the serum collected was stored at 4°C and subjected to auto analyzer for analyzing complete lipid profile and complete blood picture. Mann–Whitney U test was used to compare mean age, mean values of lipid profile parameters, and hematological parameters between OSMF and healthy group. Results: The serum lipid levels were significantly lower in patients with OSMF than in controls, and also patients with OSMF showed significantly lower levels of hemoglobin (Hb), red blood cells (RBCs), and platelets and increased levels of erythrocyte sedimentation rate (ESR) and white blood cell (WBC) than in controls. Conclusion: This study concluded that the lower serum lipid levels may serve as a diagnostic and prognostic indicator in the early diagnosis of oral premalignant lesions, and the findings in our study emphasize on the assessment of Hb, RBCs, WBCs, platelets, and ESR for patients with OSMF. So it is recommended to subject the patients with oral precancerous conditions to biochemical investigations as it may aid in early diagnosis and prognosis.

Evaluation of serum lipid profile in female patients with hypothyroidism: a rural based study

<p class="abstract"><strong>Background:</strong> Hypothyroidism is the most common of thyroid disorders in India affecting one in ten adults and more so in women than men by 6 to 8 times with overall prevalence accounting to 11%.</p><p class="abstract"><strong>Methods:</strong> This hospital based prospective study was conducted in the outpatient ENT Department of district hospital, Koppal Institute Of Medical Sciences, Koppal, Karnataka between January to June 2019 which involved total 60 Female patients out of which 20 patients were euthyroid controls and the remaining 40 patients were having one or more clinical manifestations of hypothyroidism who were divided into group A (n=20, thyroid stimulating hormone (TSH) between 5 and 10 mIU/ml) and group B (n=20, TSH >10 mIU/ml) based on TSH values. All 60 patients were in the age group of 35 to 55 years with a mean age of 42 years. Objective of study were to estimate serum lipids in female patients clinically diagnosed with hypothyroidism, to quantify the effect of lipid lowering drug and L-thyroxine therapy on TSH and serum lipid profiles. </p><p class="abstract"><strong>Results:</strong> Mean total cholesterol, low-density lipoprotein cholesterol and triglycerides level in cases was higher than controls whereas high-density lipoprotein cholesterol was found significantly decreased in cases compared to controls.</p><p class="abstract"><strong>Conclusions:</strong> In hypothyroid patients with TSH <10 mIU/ml, Lipid lowering drug seemed to be sufficient to treat dyslipidemia. While in all hypothyroid patients with TSH >10 mIU/ml, it’s imperative to institute L-thyroxine therapy for restoration of TSH and altered lipids back to normal levels.</p><p> </p>

Anti-hypercholesterolemic effect of milk produced from quaffing dairy goats with linseed oil on lipid profile of albino rats

The aim of the present study is to evaluate the effect of modified milk production from goats administrated different amounts of flaxseed oil on serum lipid profile of rats. Twenty-five mature Albino rats were randomly and equally divided into five groups, five rats each. The rats were acclimatized on basal diet for 7 days before starting the experiment. The first group was fed on basal diet and served as control I, the second group was offered basal diet plus normal goat milk (control II), while the rest three groups were fed on basal diet plus milk from goats received 10, 20 and 30 mL flaxseed oil/head/day. Results of serum analysis revealed that serum triglycerides concentrations reduced by 1.42, 6.57 and 14.10% in tested groups G1, G2 and G3 respectively, compared to control II. Moreover, total cholesterol levels were linearly decreased after 28 days of feeding, with reduction values varied from 1.39 to 9.10% with a mean value of 5.48% for treated group, compared to control II. Continuously, LDL-cholesterol levels were decreased by 13.81 and 16.49 in G2 and G3 group respectively, as compared with control II. While no significant was detected for G1 group. However, the same trend of result was also true for VLDL-cholesterol levels. In contrary, at the end of experiment, the levels of HDL–cholesterol as anti-atherogenic index were increased in all treatments compared to their corresponding initial values. In conclusion, flaxseed oil can be used as nutritional supplement to lactating goats to reduce saturated fatty acids and increase polyunsaturated fatty acids in the resultant milk.

Determination and Evaluation of Serum Lipid Profile, Creatinine and Glycated hemoglobin (HbA1c %) in Sudanese Patients with Type 2 Diabetes Mellitus Associated with Obesity

Determination and Evaluation of Serum Lipid Profile, Creatinine and Glycated hemoglobin (HbA1c %) in Sudanese Patients with Type 2 Diabetes Mellitus Associated with Obesity

<i>Linum usitatissimum</i> (Flaxseed) Oil during Postpartum Period Contributes to Lean Mass and Healthy Serum Lipid Profile in Rats

There is a dearth of efficient strategies to support postpartum among women in order to protect them from chronic diseases in later life. Flaxseed oil (FO) is a food derived oil that has functional ingredients as alpha-linolenic acid (ALA). The objective of this study was to investigate the influence of FO during postpartum period in body parameters and lipid profile in rats. After the delivery of their pups, rats were randomly divided into two groups: control – diet with soybean oil as fat source- or FO – diet with FO as a fat source. After 51 days offering experimental diets, each group was evaluated on body composition, intra-abdominal fat, serum lipid profile and polyunsaturated fatty acids. The diet based on FO recorded high serum levels of ALA (P<0.0001) and eicosapentaenoic fatty acids (P<0.05). The diet also recorded a decrease in gamma-linolenic (P<0.05), dihomo-gamma-linolenic (P<0.05) and arachidonic fatty acids (P<0.0001). These aforementioned results lead to the activation of metabolic and physiologic pathways that provided higher lean mass (P<0.05), lower results on total cholesterol (P<0.05) and low density cholesterol (P<0.05). Hence, consumption of FO during postpartum can promote lean mass and healthy body composition, better lipid profile and contribute to chronic disease prevention.