Evaluation Of Genetic Diversity Research Articles

Evaluation of genetic diversity of some Zucchini yellow mosaic virus-infected cucurbits based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, inter simple sequence repeat and start codon targeted molecular tools

Evaluation of genetic diversity of some Zucchini yellow mosaic virus-infected cucurbits based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, inter simple sequence repeat and start codon targeted molecular tools

Evaluation of Molecular Genetic Diversity and DNA Barcoding of Rita gogra (Family Bagridae) from Narmada River Revealed through Mitochondrial COX1 Gene Sequencing

Evaluation of Molecular Genetic Diversity and DNA Barcoding of Rita gogra (Family Bagridae) from Narmada River Revealed through Mitochondrial COX1 Gene Sequencing

Genetic diversity of grain yield traits and identification of a grain weight gene SiTGW6 in foxtail millet.

Agronomic traits were evaluated in 1250 foxtail millet accessions, and a crucial gene SiTGW6 governing grain yield was identified. Elite haplotypes and dCAPS markers developed for SiTGW6 facilitate molecular breeding. A comprehensive evaluation of phenotypic characteristics and genetic diversity in germplasm resources are important for gene discovery and breeding improvements. In this study, we conducted a comprehensive evaluation of 1250 foxtail millet varieties, assessing seven grain yield-related traits and fourteen common agronomic traits over two years. Principal component analysis, correlation analysis, and cluster analysis revealed a strong positive correlation between 1000-grain weight and grain width with grain yield, emphasizing their importance in foxtail millet breeding. Additionally, we found that panicle weight positively correlated with 1000-grain weight but negatively correlated with branch and tiller numbers, indicating selection factors during domestication and breeding. Using this information, we identified 27 germplasm resources suitable for high-yield foxtail millet breeding. Furthermore, through an integration of haplotype variations and phenotype association analysis, we pinpointed a crucial gene, SiTGW6, responsible for governing grain yield in foxtail millet. SiTGW6 encodes an IAA-glucose hydrolase, primarily localized in the cytoplasm and predominantly expressed in flowering panicles. Employing RNAseq analysis, we identified 1439 differentially expressed genes across various SiTGW6 haplotypes. Functional enrichment analysis indicating that SiTGW6 regulates grain yield through the orchestration of auxin and glucan metabolism, as well as plant hormone signaling pathways. Additionally, we have identified elite haplotypes and developed dCAPS markers for SiTGW6, providing valuable technical tools to facilitate molecular breeding efforts in foxtail millet.

Integrating principal component and regression analyses for genetic diversity and trait evaluation in oat genotypes

Integrating principal component and regression analyses for genetic diversity and trait evaluation in oat genotypes

Evaluation of genetic diversity in Jordanian Solanum nigrum plants and genetic stability of invitro grown plant using (AFLP) technique

Evaluation of genetic diversity in Jordanian Solanum nigrum plants and genetic stability of invitro grown plant using (AFLP) technique

Evaluation of Genetic Diversity and Agronomic Traits of Germplasm Resources of Stropharia rugosoannulata

China possesses abundant wild germplasm resources and a wide range of cultivated strains of Stropharia rugosoannulata. However, issues such as genetic diversity and unclear genetic relationships have had an impact on the classification and preservation of germplasm resources, the breeding of new varieties, and the promotion of superior strains. There is an urgent need for genetic diversity analysis and assessment of germplasm resources. In this study, we conducted whole-genome resequencing of 50 cultivated and wild strains collected from various regions across the country. After applying a series of filtering parameters, we obtained 888,536 high-quality Single Nucleotide Polymorphism (SNP) markers. Using these SNP markers, we performed principal component analysis, population structure analysis, and phylogenetic clusters analysis on the 50 strains. Most cultivated strains exhibited high genetic similarity, while significant genetic diversity was observed among wild strains. Based on factors such as marker distribution throughout the entire genome and marker quality, we selected 358 core SNP markers to construct SNP fingerprints. Two-dimensional barcodes were generated for each strain to enable specific identification. Additionally, the mycelial growth rate of strains was evaluated on Potato Dextrose Agar (PDA) and substrate culture media. We also assessed their lignin degradation capability using guaiacol agar plates assay. It was observed that the mycelial growth rate on PDA and substrate culture medium exhibited a significant correlation with the diameter of the mycelial colony on guaiacol agar medium. Additionally, the correlation between the mycelial growth rate on the substrate medium and that on the guaiacol agar plate was stronger than that on PDA medium. This study provided molecular-level identification and assessment of germplasm resources. It clarified the genetic relationships among strains and the characteristics of mycelium growth-related agronomic traits of each strain. This research contributed to the enrichment and development of utilizable germplasm resources and breeding materials for S. rugosoannulata, offering a scientific basis for further research.

Evaluation of Genetic Diversity among Proton Beam Induced Wheat Mutants through Trait Association and Multivariate Analysis

Evaluation of Genetic Diversity among Proton Beam Induced Wheat Mutants through Trait Association and Multivariate Analysis

Evaluation of the durum wheat landrace genetic diversity using agro analysis and its benefit for human health

Ancient wheat and landraces (traditional varieties) are fascinating to the consideration of scientists who are re-evaluating the healthy and dietary properties attributed to them by popular tradition. Wheat (Triticum durum) is one of the most significant crops that is grown all over the world. In the present study, 19 landraces were analyzed for genetic diversity through morphological and SDS-PAGE analysis. The highest coefficient of variance (18.91%) was found for spike length, and the lowest for days to maturity (1.51%). The genotype Malakand and Dolram produced a maximum number of seeds (42 and 46 seeds per plant), and the genotypes Dogai and Rasool Banda were found to be the tallest (73 and 71 cm, respectively). The genotype Kamar Kotki was found to mature early (165 days) while Doagi was found to mature late (171 days). Spike length was found to be positively correlated with plant height, leaf width, and leaf length. Leaf width was significant with spike length and the internode distance. The cluster analysis divided all the genotypes into six sub-clusters where the Asban and the Shekhan were placed at the extreme of the dendrogram. Based on the total seed storage proteins analysis, 18 (85.71%) bands were polymorphic while 14.28% bands were found to be monomorphic. Using Ward’s methods, all the genotypes were divided into two main lineages at 25% Euclidian distance and five sub-clusters at 75% distance. Our findings have significance for understanding the variations and relationships between various durum wheat genotypes and could serve as a basis for local elite durum germplasm that has been regionally adapted.

Evaluation of Genetic Diversity in Wild Rhynchosia capitata through Morphomatric and Biochemical Character Collected from District Dir Lower

Evaluation of Genetic Diversity in Wild Rhynchosia capitata through Morphomatric and Biochemical Character Collected from District Dir Lower

Unraveling Diversity and Character Association in Sesame (Sesamum indicum L.) using Different Agro-Morphological Traits

Sesame is an important oilseed crop, carrying several beneficial properties to human kind. Given its wide-ranging applications, there is a constant need for enhancing the seed yield. Evaluation of genetic diversity and character association among various seed yield components is considered as the fundamental steps in any breeding program. Thus, the present study has been focused to investigate the diversity and character associations of twenty different sesame genotypes, using different agro-morphological traits. The diversity analysis conducted in this study, unveiled the presence of two distinct clusters. The first cluster encompassed three genotypes, while the remaining seventeen genotypes belonged to the second cluster. Furthermore, character associations were explored through correlation analysis, and subsequently partitioned into direct and indirect effects through path analysis. Correlation study revealed that seed yield per plant (SYP) was positively correlated with the capsules per plant (CPP), biological yield per plant (BYP) and harvest index (HI), while negative correlation was observed with days to 50% flowering (DFF) and days to maturity (DM). Through path analysis, we found that positive direct effects on SYP were observed for BYP, HI, DM, plant height (PH) and test weight (TW). Conversely, negative direct effects on SYP were noted for CPP, BPP and DFF. These findings highlighted the importance of focusing on BYP, HI, and CPP during the selection process, as these factors were identified as important components for seed yield. The insights gained from this study will not only aid in the selection of suitable parents for hybridization, but also assist in determining the key seed yield components for sesame improvement.

Evaluation of Genetic Diversity and Zinc Deficiency Stress Tolerance in Spring Wheat Cultivars

Evaluation of Genetic Diversity and Zinc Deficiency Stress Tolerance in Spring Wheat Cultivars

Genetic diversity assessment and in vitro propagation of some date palm (Phoenix dactylifera L.) varieties

The evaluation of genetic diversity is crucial for breeders to develop strategies and improve the resilience, quality, and adaptability of the date palm. In this study, the genetic diversity of three date palm varieties was performed using ISSR-PCR molecular markers to determine its relationship with in vitro propagation response of these varieties. The molecular profiling was performed using ISSR-PCR. A total of 49 loci were produced by the PCR reactions, 38 of which were polymorphic while 11 were monomorphic. The level of polymorphism revealed by ISSR-PCR varied from 33.33% to 100%. The three date palm varieties were grouped into two clusters based on the results of cluster analyses that used morphological data and molecular profiles. Cluster I comprised the ‘Barhy’ variety and Cluster II included ‘Magdoul’ and ‘Amri’ varieties. The clustering analyses revealed the independence of the ‘Barhy’ variety in its characteristics from the other varieties based on either morphological or molecular data. The results of in vitro propagation showed that the ‘Amri’ variety exhibited the highest callus induction frequency (86.28%), callus weight (2.33 g), number of somatic embryos (9.32), number of shoots (14.62), number of roots (4.11), root length (4.63 cm), shoot length (13.61 cm) followed by ‘Magdoul’ and ‘Barhy’ varieties. The ‘Amri’ variety had the shortest callus induction period, at 23.26 days while the ‘Barhy’ variety exhibited the longest period of callus induction (28.55). It was deduced from the study that the ISSR marker reproduced trustworthy patterns of bands to determine the genetic diversity among different date palm varieties that are considered the cornerstone for the genetic improvement of date palms. The understanding of the relationship between genetic diversity and in vitro propagation response of date palm is essential for ensuring the long-term sustainability of its crop. This will facilitate better conservation and development of new date palm varieties that fulfil the needs of farmers and consumers.

Evaluation of Genetic Diversity in Quill Mites of the Genus Syringophiloidus Kethley, 1970 (Prostigmata: Syringophilidae) with Six New-to-Science Species.

Quill mites (Acariformes: Syringophilidae) are poorly explored bird parasites. Syringophiloidus Kethley, 1970, is the most specious and widespread genus in this family. It is believed to contain mono-, steno- and poly-xenous parasites and thus seems to be an exemplary for studies on biodiversity and host associations. In this work, we applied the DNA barcode marker (mitochondrial cytochrome c oxidase subunit I gene fragment, COI) to analyze the species composition and host specificity of representatives of fifteen Syringophiloidus populations parasitizing fifteen bird species. The neighbor joining analyses distinguished thirteen monophyletic lineages, almost completely corresponding to seven previously known species recognized based on morphological features, and six new-to-science species. The only exception is S. amazilia Skoracki, 2017, which is most likely conspecific with Syringophiloidus stawarczyki Skoracki, 2004. The intraspecific distances of all species were not higher than 0.9%, whilst the interspecific diversity ranged from 5.9% to 19.2% and 6.3-22.4%, inferred as the distances p and K2P, respectively. Although all putative species (except S. amazilia) are highly supported, the relationships between them have not been fully resolved and only faintly indicate that both host phylogeny and distributions influence the phylogenetic structure of quill mite taxa.

SSR-marker assisted evaluation of genetic diversity in local and exotic pigeonpea cultivars in Benin for parental genotypes selection

Pigeonpea production is limited in Benin due to the undesirable agronomic and organoleptic characteristics of cultivated landraces. For a breeding program, some exotic cultivars were introduced for improving the available genepool in the country. The present study aims to assess the genetic diversity within a collection comprising of 32 local and 28 exotic cultivars, with the objective of selecting suitable parental lines for pigeonpea genetic improvement. All of the 60 accessions were genotyped with 14 SSR makers and a total of 38 alleles were detected with an average of 2.71 alleles per locus. The mean value of PIC (Polymorphism Information Content) and overall gene diversity were 0.37 and 0.43, respectively, while the mean value of heterozygosity was 0.11, indicating moderate genetic diversity within the 60 accessions with a deficit of heterozygous genotypes. As expected, the diversity was higher in exotic cultivars than in local landraces. Based on the genetic relationship among accessions, the entire collection formed 3 clusters, one of which comprised four specific exotic accessions. Population structure analysis using model-based and DAPC (Discriminant Analysis for Principal Components) methods subdivided the 60 accessions into two major groups reflecting their geographic origins with a minimal number (02) of admixtures. This indicates significant genetic divergence between local and exotic cultivars. Analysis of Molecular Variance (AMOVA) results with an overall Fst value of 0.13 confirmed the significant genetic divergence between the two collections. The findings of this study will significantly benefit for pigeonpea breeding programs in Benin. For practical applications, selected exotic cultivars, particularly those within the specific cluster can immediately serve as donor parents in crossbreeding schemes with the local landraces, for developing new varieties.

Association Mapping for Evaluation of Population Structure, Genetic Diversity, and Physiochemical Traits in Drought-Stressed Maize Germplasm Using SSR Markers.

Globally, maize is one of the most consumed crops along with rice and wheat. However, maize is sensitive to different abiotic stress factors, such as drought, which have a significant impact on its production. The aims of this study were to investigate (1) genetic variation among 41 maize-inbred lines and the relationships among them and (2) significant marker-trait associations (SMTAs) between 7 selected physiochemical traits and 200 simple sequence repeat (SSR) markers to examine the genetics of these traits. A total of 1023 alleles were identified among the 41 maize-inbred lines using the 200 SSR loci, with a mean of 5.1 alleles per locus. The average major allele frequency, gene diversity, and polymorphism information content were 0.498, 0.627, and 0.579, respectively. The population structure analysis based on the 200 SSR loci divided the maize germplasm into two primary groups with an admixed group. Moreover, this study identified, respectively, 85 SMTAs and 31 SMTAs using a general linear model (Q GLM) and a mixed linear model (Q + K MLM) with statistically significant (p < 0.05 and <0.01) associations with the seven physiochemical traits (caffeic acid content, chlorogenic acid content, gallic acid content, ferulic acid content, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, leaf relative moisture content, total phenolic content). These SSR markers were highly correlated with one or more of the seven physiochemical traits. This study provides insights into the genetics of the 41 maize-inbred lines and their seven physiochemical traits and will be of assistance to breeders in the marker-assisted selection of maize for breeding programs.

Evaluation of Genetic Diversity and Phylogenetic Relationship among the Major Banana Varieties of North-Eastern India Using ISSR, IRAP, and SCoT markers

Evaluation of Genetic Diversity and Phylogenetic Relationship among the Major Banana Varieties of North-Eastern India Using ISSR, IRAP, and SCoT markers

First evaluation of genetic diversity among honeybee populations in Kazakhstan

Beekeeping as a staple of agriculture in Kazakhstan is believed to have emerged when external bee colonies were introduced into the country. To date, six subspecies have been distributed across commercial apiaries (Apis mellifera sossimai, A. m. carpatica, A. m. mellifera, A. m. ligustica, A. m. caucasica, and A. m. carnica). In this study, we analyzed mitochondrial DNA from 21 geographically distinct populations with diverse subspecies composition and discovered 19 unique haplotypes belonging to C and M evolutionary lineages. Analysis of 9 microsatellite loci revealed strong polymorphism, with a range of 5 to 35 alleles at each locus. The observed heterozygosity across the populations varied from 0.2892 to 0.9099, and clustering analysis only allowed differentiation of each population based on its geographical location. High degree of admixture among subspecies was identified in every population, including a potential introgression from the endemic bee A. m. pomonella. Unregulated cross-breeding for the past 50 years has laid the foundation for the promiscuous genetic nature of honeybee populations in Kazakhstan.

Molecular Marker-Based Identification and Genetic Diversity Evaluation of Fusarium spp. - А Review

The Fusarium genus encompasses filamentous fungi of global significance in agriculture, ecology, and the food industry. Certain members of this genus act as plant pathogens, leading to substantial fungal infections in crucial crops, especially cereals. Many Fusarium species produce mycotoxins that are species or even strain-specific, contaminating the food chain and posing risks to both animal and human health. Identifying Fusarium species remains a formidable challenge due to the dynamic taxonomy within the ge¬nus. Traditional identification relies on biological, phylogenetic, and morphological criteria. However, mod¬ern genomic analysis techniques have revolutionized species identification, including that of Fusarium spp. Established molecular markers like the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA-ITS1), Translation elongation factor 1-α (TEF1-α), Intergenic spacer region of rDNA (ISG), and Beta-tubulin (β-tub) facilitate precise genetic identification. The “DNA barcoding” method, recognized in scientific literature, offers a meticulous approach to molecular identification and taxonomic classification of Fusarium species. Integrating traditional identification methods with contemporary DNA analysis tech-niques broadens the possibilities for discerning and establishing phylogenetic relationships among closely related mycotoxigenic species. This integrated approach greatly aids in the development of effective control and management strategies.

Genome-Wide Development of Polymorphic SNP Markers and Evaluation of Genetic Diversity of Litchi (Litchi chinensis Sonn.)

Genome-Wide Development of Polymorphic SNP Markers and Evaluation of Genetic Diversity of Litchi (Litchi chinensis Sonn.)

Evaluation of genetic diversity in different populations of six Salvia species using R-ISSR markers analysis

Evaluation of genetic diversity in different populations of six Salvia species using R-ISSR markers analysis