The accuracy and reliability of passenger evacuation simulation remain crucial in the research field of maritime pedestrian safety. However, a noticeable gap exists between current simulation results and actual pedestrian evacuation behaviors. This paper aims to enhance the precision of evacuation simulation result by gathering passenger behavioral data through on-ship evacuation experiments. The data collected during the evacuation are integrated with an evacuation simulation tool, and the pedestrian evacuation processes in both the experiment and simulation are analyzed and compared. The results reveal that the simulation tool has certain limitations, particularly in replicating passengers’ route selection and other specific behaviors. Nonetheless, when the evacuation experiment data is incorporated, there is a marked improvement in the congruency between the simulation and experiment results, compared to the use of the simulation tool alone. Therefore, incorporating drill data into the simulation scenario design presents a viable method to boost the accuracy of the simulation result. The findings of this study have practical implications for improving the accuracy of evacuation simulations and enhancing passenger safety on ships.