Mobile charging station (MCS) is a brand-new technology to electric vehicle charging, which is a supplementary service for addressing the shortcomings associated with fixed charging station (FCS), such as prolonged waiting time of charging, charging congestion of FCS, and user travel anxiety. While MCSs offer convenience to electric vehicle users, the challenge faced in the MCS operation is that MCS equipped with large batteries to provide charging services to users by visiting them one by one not only leads to high operating costs, but also prolongs the waiting time for users to utilize MCS services. To overcome the issues, a novel framework is proposed by optimizing the MCS operation in economic efficiency. In this study, a variant of mixed integer linear programming (MILP) model is developed to maximize the MCS operator's profits with considering the user's perspective, including clustering, and covering user demands, setting temporary charging location for users, dispatching MCSs for charging and discharging, and scheduling EV charging. As the scale of the problem increases, the solution time of using the proposed MILP model is inefficient. Whereas an improved genetic algorithm is developed for solving a large-scale of the proposed model. Solomon's benchmarking instance data is adopted to evaluate the performance and validity of the proposed algorithm, complemented by various sensitivity analyses aimed at providing managerial insights into MCS operations.