Introduction: Eustachian tube dysfunction (ETD) is a well-known co-morbid manifestation of inflammatory diseases of the nasal cavity. Allergic rhinitis (AR) is a common chronic condition that causes a significant amount of disease burden in the population. However, studies on ETD in patients with AR are still very limited. Materials and methods: A total of 60 healthy and 60 AR patients participated in the prospective comparative study. All of the subjects were subjected to a skin prick test. According to ARIA criteria, the severity of AR was classified as "mild" or "moderate-severe" and "persistent" or "intermittent." Eustachian Tube Dysfunction Questionnaire (ETDQ-7) subjective scores were obtained for both groups. For objective examinations, nasal cytology, Tympanometry, and Tubomanometry were used, and the data were statistically analyzed. Results: The mean eosinophil and neutrophil counts in nasal smears demonstrated a significant difference between the two groups. In the AR and control groups, the overall ETDQ-7 score was 21.6 and 9.7 respectively (p<0.001). Tympanometry reports that ETD was significantly higher in the AR group (83.3%) than in the control group (p<0.001). TMM also established that 84.2% of AR subjects had ETD, which was statistically significant.