The peculiarities of the attitude of the two leading Czech political parties regarding the process of European integration through the prism of the effectiveness of the protection of national interests at the supranational level are analyzed in the given article. While conducting this study, it was revealed, that once it became clear that accession to the European Union would inevitably require certain national concessions from the Czech Republic, the leading Civic Democratic Party began to declare an increasingly Eurosceptic position. Gradually, EU membership was considered by it only through the prism of realizing the economic interests of the country. Civic Democrats have become even more vigorous opponents of deepening European integration and have strongly opposed the adoption of a common EU Constitution and federalization and have opposed any attempt to alienate part of national sovereignty in favor of the EU, since the Czech Republic joined the European Union. The author emphasizes that the disapproval by CDP of further European integration is explained by the historical fears of many Czechs, who are convinced that the development of the EU in a federal direction is in line with German interests and aspirations to dominate in Europe. The author also stresses on the fact, that the anti-German attitude of the Civic Democrats stems from the history of relations between two nations which have never been friendly and is explained by fears about further economic expansion of Germany in the Czech Republic. It was found that the discourse of Czech Communists on European integration is quite autonomous, because, in contrast to the CDP (and in general to all other parliamentary parties of the country), which although remains critical of the EU, but it does not question the Czech Republic membership in the European Union, the Communist Party does not consider any prospects for the functioning of the EU at all, and it does not consider the expediency of Czech Prepublic membership in it. Key words: Czech Republic; European integration; Civic Democratic Party; Communist Party of the Czech Republic and Moravia; Euroscepticism; Autonomist discourse.