Eucalyptus Species Research Articles

Genetic structure and diversity in wild and breeding populations of Eucalyptus urophylla

Abstract Eucalyptus urophylla S.T. Blake is a species of great commercial importance, especially in tropical regions, and it is the main eucalypts species cultivated in Brazil. This study evaluated the genetic diversity among and within seven populations of E. urophylla and estimated the genetic distance between individuals to draw inferences about the genetic structure between and within the sampled populations. For that, 19 microsatellite markers were genotyped in 254 individuals originating from four wild populations, introduced in Brazil, two breeding populations, and one population consisting of commercial clones. The wild populations of E. urophylla introduced in Brazil have high genetic similarity and the few generations of breeding have already generated significant differences in population structure between improved and wild populations. As expected, breeding populations are closer to commercial clones than wild populations. However, compared to wild populations, breeding populations exhibit greater genetic diversity as they originated from a mixture of provenances. The population formed by clones was the only one that showed a negative Wright fixation index, that is, heterozygosity was higher than expected for a population in Hardy-Weinberg equilibrium.

Effects of enrichmemt planting with native tree species on bacterial community structure and potential impact on Eucalyptus plantations in southern China

Multi-generational planting of Eucalyptus species degrades soil quality but the introduction of legumes can improve soil fertility and microbial diversity. However, the effects of introducing non-legume native tree species on soil nutrients and bacterial community structure remain poorly understood. This study investigated the impacts of the conversion of third generation monoculture Eucalyptus plantations to mixed systems including Eucalyptus urograndis with Cinnamomum camphora (EC) and E. urograndis with Castanopsis hystrix (EH), on soil chemical and biochemical properties and bacterial community structure, diversity and functions. First generation E. urophylla plantations were the control. Results show that planting the third generation Eucalyptus led to a significant decrease in pH, organic matter, nutrient content, enzyme activities (invertin, acid phosphataes, and urease), and bacterial α-diversity compare to the controls. However, the mixed planting showed significant improvement in soil chemical and biochemical attributes and bacterial α-diversity, although the E. urograndis and C. hystrix planting had no improvement. Chloroflexi (oligotrophic bacteria) were significantly enriched in third generation Eucalyptus and Eucalyptus + C. hystrix, while proteobacteria increased significantly in the E. urograndis with C. camphora plantings. The relative abundance of multiple metabolic pathways increased significantly in the third generation Eucalyptus plantations whereas membrane transport-related genes were enriched in soils of the mixed systems. The changes in bacterial community structures in the two mixed systems were driven by diversity, organic matter and acid phosphatase, while bacterial functions were affected by invertase, {mathrm{NO}}_{3}^{-}-N, diversity and urease. These results suggest that the transformation of successive monoculture Eucalyptus plantations into mixed plantations reduces the depletion of soil nutrients and enhances the ecological function of soil microorganisms.

Assessment of Usage Diversity of Agroforestry Tree Species in Hadiya Zone, Southern Ethiopia

Diversification of products and services from agroforestry trees of Ethiopia becomeimportant for sustainable farming systems. This study was conducted with the aim to investigate usagediversity of tree species. At farm level different tree species were counted, listedand recoded. The DBH of all trees and shrubs ≥5cm was measured. The average basal area per farm was 11 m2 (12 m2 per ha), with a range between 0.74 –59 m2 per farm (2.3–35.8 m2 per ha).Sixteen different usage typeswere identified, of which fuel wood holds the largest percent followed by both pole and medicinal use.Different 65 species of trees were recordedin terms of the mean important Value Index (IVI), of which Eucalyptus species ranked first (57), followed by Cordiaafricana (50.4), Croton macrostachyus (33),Erythrinaabyssinica (31.4), and Cupressuslusitanica (19). The IVI of these five species accounted for more than 30% of the total IVI. Usage diversity is well recognized in the study area but further studies of trees and use diversity in relation to soil fertility in particular and environmental Management in general seems important.

Common ringtail possums (

Plant secondary metabolites (PSMs) can influence the consumption of particular plants by herbivores. Unsubstituted B-ring flavanones (UBFs), which are compounds found in the leaves of Eucalyptus species from the Eucalyptus subgenus (common name monocalypt), deter feeding by common brushtail possums (Trichosurus vulpecula) when added to artificial diets at concentrations that reflect those found naturally in foliage. We hypothesised that common ringtail possums (Pseudocheirus peregrinus) would tolerate higher UBF concentrations, because, unlike brushtail possums, they regularly eat monocalypt foliage. This proved to be the case; ringtail possums were not deterred by two different UBFs, pinocembrin and flavanone, at concentrations up to 4.7% dry matter, which is substantially higher than the concentrations that deter brushtail possums. These results extend and refine our understanding of how variation in tolerance or susceptibility to eucalypt PSMs affects the diets of sympatric marsupial folivores, potentially reducing competition for food.

Eucalypt in the Tunisian arid region: Diversity and valorization for honey production

For four years (2016 - 2019), we studied Eucalyptus in the arid region of Tunisia. This tree is frequently introduced in the region from the sixties of the twentieth century, with the installation of three arboreta in 1959/1960 and several plantations; these arboreta are the only ones planted in the arid region. Fifteen species and one hybrid have been identified, with a very variable presence, the most dominant species are: E. camaldulensis, E. torquate, E. microtheca, E. occidentalis and E. gomphocephala. In 2017 and 2018, each month, we have followed the flowering of all Eucalyptus species, identified those that have an importance and abundance of flowering, and qualified as interesting in beekeeping. These are: E. camaldulensis, E. torquata, E. microtheca, E. occidentalis, E. oleosa, E. diversifolia, E. flocktoniae and E. torwood (hybrid). Besides, other species are present and show no interest for beekeepers, mainly E. gracilis, E. astringens, E. brockwayii and E. gomphocephala, the latter is very common in Mediterranean region, Tunisia and the area of this study (Gabes).

Adaptability and interspecific variability in growth and leaf traits of eucalypt

Effective adaptability of plants to new environments can be analysed in terms of survival rate. Analysing the traits that favour adaptation to environmental changes provides a more in-depth understanding of the mechanisms involved. Local adaptation occurs because different environmental factors exert selective pressure across habitats. Understanding the leaf mechanisms underlying plant survival and growth is crucial to determine why local adaptation involves trade-offs. A comparative provenance test on 29 eucalyptus species was conducted to improve our understanding of species adaptation strategies on coastal plains of Pointe-Noire, Republic of the Congo. We studied the different functional traits to determine how plants function and to highlight the different species’ adaptive strategies. For each species, survival, growth traits and leaf traits were measured, and the climatic factors of the origin area for each species was taken into account. Cluster analysis was performed on groups of species with a similar growth strategy. The results revealed general trends that explain the physiological mechanisms involved in the species’ local adaptation. Indeed, species have survived to current environmental changes by adjusting their specific leaf area plasticity. The 32 provenances of eucalyptus were subdivided into four groups by cluster analysis. The first cluster included two species (E. pilularis and E. peltata) that are totally unsuited to the local conditions in Pointe-Noire, with the slowest growth rate and smallest specific leaf area. The second cluster contained species that showed a wide variety of growing strategies, allowing them to adapt to local conditions. The third cluster included a species that is specialised in obtaining large quantities of resources, while investing very little in growth. The fourth cluster included species that acquired and used resources at a slow rate. Leaf anatomy was quite responsive to climatic conditions. We evaluated the different strategies and found that eucalyptus species had very diverse functional traits, which may explain their broad ecological range.

Growth and Productivity Modeling of Seven Eucalyptus Species in Souiniet’s Arboretum in the Northwestern of Tunisia

This study consists of the development of tree growth models to deduce stands productivity and determine the highest productive species in the conditions of the concerned plantation. Seven Eucalyptus, introduced in the arboretum of Souiniet (north-west of Tunisia, wet Mediterranean bioclimate) in a Cork Oak natural forest, were studied. Stem analysis and non-linear growth modeling regression equations were used to predict wood productivities. Gompertz and Chapman–Richards growth function appeared as being great numerical models to estimate the Eucalyptus tree diameter and height evolutions, respectively. Moreover, an adapted Chapman–Richards model allowed predicting the volume of trees in an efficient manner. The values of mean annual volume productivity of the Eucalyptus spp.studied, allow us to classify them in order of increasing annual productivity, as follows: E. sideroxylon, E. cinerea, E. maidenii, E. macrorhyncha, E. tereticornis, E. viminalis and E. bicostata. The first three Eucalyptus spp. appeared as the best-adapted and most suitable Eucalyptus trees for new plantations in this area. These species had the highest mean annual increments, ranged from 5 to 10 m3.ha−1.year−1 with 15 to 20 years of rotation. E. bicostata is the most promising, with annual average production exceeding 10 m3.ha−1.year−1 after 25 years, and reaching 20 m3.ha−1.year−1 at 40 years old. These modeling approaches provide additional knowledge on the productivity of the different Eucalyptus species, thus enabling forestry operators to simulate the development of forest stands in order to optimize timber production and harvesting.

A Modified Lysimeter Study for Phyto-Treatment of Moderately Saline Wastewater Using Plant-Derived Filter Bedding Materials.

The present study focuses on determining the phyto-treatment efficiency for treatment of moderately saline wastewater using organic raw materials, such as rice husk, coconut husk, rice straw, and charcoal. The moderately saline wastewater with total dissolved solids (TDS) concentration up to 6143.33 ± 5.77 mg/L was applied to the lysimeters at the rate of 200 m3 ha–1 day–1 in five different lysimeter treatments planted with Eucalyptus camaldulensis (T1, T2, T3, T4, and T5). T1 was a control without any filter bedding material, whereas rice straw, rice husk, coconut husk, and charcoal were used as filter bedding materials in the T2, T3, T4, and T5 treatment systems, respectively. Each treatment showed significant treatment efficiency wherein T3 had the highest removal efficiency of 76.21% followed by T4 (67.57%), T5 (65.18%), T2 (46.46%), and T1 (45.5%). T3 and T4 also showed higher salt accumulation, such as sodium (Na) and potassium (K). Further, the pollution load in terms of TDS and chemical and biological oxygen demand significantly reduced from leachate in the T3 and T4 treatments in comparison with other treatments. Parameters of the soil, such as electrical conductivity, exchangeable sodium percentage, and cation exchange capacity did not show values corresponding to high salinity or sodic soils, and therefore, no adverse impact on soil was observed in the present study. Also, Eucalyptus camaldulensis plant species showed good response to wastewater treatment in terms of growth parameters, such as root/shoot weight and nitrogen, phosphorus, and potassium (NPK) uptake, plant height, biomass, and chlorophyll content. Root and shoot dry weight were in the order T3 (51.2 and 44.6 g)>T4 (49.3 and 43.5 g) > T5 (47.6 and 40.5 g) > T2 (46.9 and 38.2 g) > T1 (45.6 and 37.1 g). Likewise, the total chlorophyll content was highest in T3 (12.6 μg/g) followed by T4 (12.3 μg/g), T5 (11.9 μg/g), T2 (11.5 μg/g), and the control, that is, T1 (11.0 μg/g). However, the most promising results were obtained for T3 and T4 treatments in comparison with the control (T1), which implies that, among all organic raw materials, coconut and rice husks showed the highest potential for salt accumulation and thereby wastewater treatment. Conclusively, the findings of the study suggest that organic raw material–based amendments are useful in managing the high salts levels in both plants and leachates.

Using permanent forest plots to evaluate the resilience to fire of Tasmania’s tall wet eucalypt forests

The vulnerability to repeated high severity fires of tall wet eucalypt forests (TWEF) dominated by obligate seeder species is widely understood. However, 80% of Tasmania’s TWEF are dominated by resprouter species, and while these forests are more resilient to wildfire than those dominated by obligate seeders, the degree of their resilience is not well documented. Recently, two wildfires affected five 1-ha forest plots that had been previously measured, providing the opportunity to assess fire effects on these forest stands. We characterised these wildfires using remote sensing and field measurements of char height (a proxy for fire intensity) and canopy scorch (fire severity). We assessed the influence of tree diameter, fire intensity and seedling densities on the survival and resprouting responses of eucalypt overstorey trees and mixed species understorey trees. Our results showed that these fires were predominantly of low to moderate intensity and severity. Our results showed that mature TWEF were resilient to these wildfires, with overall eucalypt survival of 75%. The major eucalypt species were either mostly not defoliated (E. regnans), or could recover rapidly by epicormic resprouting (E. delegatensis and E. obliqua). Results from this and other studies show recovery from topkill caused by high severity fires occurs but is slower, by basal resprouting (E. obliqua) or from seed (all species). By contrast, understorey species suffered high mortality (85% overall), with few species showing substantial resprouting. Fire resistance in both groups increased markedly with tree diameter.Our results have implications for forest management. The high survival of overstorey eucalypts leads to a multi-aged stand structure in most unlogged old growth TWEF in Tasmania. On the other hand, clearfell logging, originally designed to mimic stand replacing wildfire in obligate seeder systems, creates a vulnerable, even aged stand of young regrowth, and at a landscape scale, it also reduces average tree size, reducing overall resistance to fire. Adopting alternatives to clearfelling, such as partial logging systems, will increase landscape resilience to fire, as well as providing other previously shown benefits.

Variation in chemical composition and biological properties of two Tunisian Eucalyptus essential oils under three eco-friendly extraction techniques

ABSTRACT In this paper, three extraction methods: conventional hydrodistillation, microwave assisted hydrodistillation and supercritical carbon dioxide extraction, were applied to isolate essential oils from two Eucalyptus species. To our knowledge, it is the first study dealing with extraction and characterization of essential oils from E. intertexta and E. diversifolia from the south of Tunisia. A comparison between species in terms of yield, extraction time, and chemical composition of essential oils as well as their anti-inflammatory and anti-Alzheimer activities was done. The obtained yields varied from 0.88 to 8.35% depending on the species and extraction process. Chemical composition of essential oils was evaluated using gas chromatography and mass spectrometry. Eighty-six compounds were identified, whose major are 1,8-cineole (60.36%), and α-pinene (24.72%) in E. diversifolia EOs and 1,8-cineole (33.50%), caryophyllene oxide (21.51%) and p-cymene (21.29%) in E. intertexta EOs. Microwave assisted hydrodistillation was shown to be the faster compared to the other studied methods. Interesting activities were observed with E. diversifolia essential oils extracted by hydrodistillation (50% inhibition in anti-inflammatory test (5-lipoxygenase)) and by microwave assisted hydrodistillation (49% inhibition in anti-Alzheimer test (acetylcholinesterase)).

Mapping canopy nitrogen-scapes to assess foraging habitat for a vulnerable arboreal folivore in mixed-species Eucalyptus forests.

Herbivore foraging decisions are closely related to plant nutritional quality. For arboreal folivores with specialized diets, such as the vulnerable greater glider (Petauroides volans), the abundance of suitable forage trees can influence habitat suitability and species occurrence. The ability to model and map foliar nitrogen would therefore enhance our understanding of folivore habitat use at finer scales. We tested whether high‐resolution multispectral imagery, collected by a lightweight and low‐cost commercial unoccupied aerial vehicle (UAV), could be used to predict total and digestible foliar nitrogen (N and digN) at the tree canopy level and forest stand‐scale from leaf‐scale chemistry measurements across a gradient of mixed‐species Eucalyptus forests in southeastern Australia. We surveyed temperate Eucalyptus forests across an elevational and topographic gradient from sea level to high elevation (50–1200 m a.s.l.) for forest structure, leaf chemistry, and greater glider occurrence. Using measures of multispectral leaf reflectance and spectral indices, we estimated N and digN and mapped N and favorable feeding habitat using machine learning algorithms. Our surveys covered 17 Eucalyptus species ranging in foliar N from 0.63% to 1.92% dry matter (DM) and digN from 0.45% to 1.73% DM. Both multispectral leaf reflectance and spectral indices were strong predictors for N and digN in model cross‐validation. At the tree level, 79% of variability between observed and predicted measures of nitrogen was explained. A spatial supervised classification model correctly identified 80% of canopy pixels associated with high N concentrations (≥1% DM). We developed a successful method for estimating foliar nitrogen of a range of temperate Eucalyptus species using UAV multispectral imagery at the tree canopy level and stand scale. The ability to spatially quantify feeding habitat using UAV imagery allows remote assessments of greater glider habitat at a scale relevant to support ground surveys, management, and conservation for the vulnerable greater glider across southeastern Australia.

Climate change and the suitability of local and non‐local species for ecosystem restoration

SummaryRestoring damaged, degraded and destroyed forest ecosystems to a target state often follows a local‐is‐best approach. However, rapidly changing climates may result in the local species no longer being the best now or into the future. This raises questions about whether there is a need to consider emerging ecosystems that bring together local and non‐local species that have not historically co‐occurred to ensure climate‐resilience of restored forest ecosystems; or whether non‐regional species may be needed to support ecosystem functionality in some areas. In this study, I present an experimental framework to assist decision‐making by evaluating the suitability of local and non‐local native species for ecosystem restoration under current and predicted climates. I applied this framework using the Tasmanian Midlands, Australia, as a case study, focusing of the diverse eucalypt flora of the island. Three key findings emerged. Firstly, the climate of the Midlands has rapidly changed across this biodiversity hotspot over the last century, and current mean annual maximum temperatures are already tracking worse case climate change scenarios. Secondly, using a combination of climate envelope modelling and habitat suitability modelling, I identified the degree to which the Midlands region provides suitable climate habitat for a range of local and non‐localEucalyptusspecies now and into the future. Thirdly, although the Midlands is predicted to support suitable climate habitat for 82% of the regionally local eucalypt species now and into the future, it is predicted that the local populations of these least vulnerable species may be at risk of climate maladaptation, particularly in the northern regions of the Midlands. Models suggests that mixing local seed with non‐local seed sources currently occupying habitat with a climate that is predicted for the Midlands will be required. The study provides a basis for managers to consider the suitability of regionally local species and identify potential areas where local seed may need to be supplemented with non‐local seed for ecosystem restoration in the face of climate change.

Site productivity indices for native forests in southeast Queensland

Site productivity, or site quality, describes the potential biomass growth and yield of vegetation at a given location. Land managers have devised indices for site productivity using attributes related to plant yields or growth rates, and these have great utility when available spatially in maps. The main factors determining site productivity include climate, soil and terrain characteristics. Here we analysed four productivity indices (two based on remote sensing only, two based on modelling and algorithms using spatial datasets). The tested indices were available over a 150,000 km2 area of southeast Queensland Australia, a region dominated by Eucalyptus and Acacia species. We were interested in comparing the indices regarding underlying drivers, effects on vegetation types and the general distribution of site productivity across our study region. Our methods included histograms of spatial attribute intersection, and multivariate linear regression. Remote sensing has clear advantages in capturing current conditions, which potential productivity algorithms cannot depict. On the other hand, maps with productivity algorithms provide large-scale robust information on biomass growth/yield that is sensitive to the main drivers of plant growth (e.g. climate, soil).

QtlXplorer: an online systems genetics browser in the Eucalyptus Genome Integrative Explorer (EucGenIE)

BackgroundAffordable high-throughput DNA and RNA sequencing technologies are allowing genomic analysis of plant and animal populations and as a result empowering new systems genetics approaches to study complex traits. The availability of intuitive tools to browse and analyze the resulting large-scale genetic and genomic datasets remain a significant challenge. Furthermore, these integrative genomics approaches require innovative methods to dissect the flow and interconnectedness of biological information underlying complex trait variation. The Plant Genome Integrative Explorer (PlantGenIE.org) is a multi-species database and domain that houses online tools for model and woody plant species including Eucalyptus. Since the Eucalyptus Genome Integrative Explorer (EucGenIE) is integrated within PlantGenIE, it shares genome and expression analysis tools previously implemented within the various subdomains (ConGenIE, PopGenIE and AtGenIE). Despite the success in setting up integrative genomics databases, online tools for systems genetics modelling and high-resolution dissection of complex trait variation in plant populations have been lacking.ResultsWe have developed qtlXplorer (https://eucgenie.org/QTLXplorer) for visualizing and exploring systems genetics data from genome-wide association studies including quantitative trait loci (QTLs) and expression-based QTL (eQTL) associations. This module allows users to, for example, find co-located QTLs and eQTLs using an interactive version of Circos, or explore underlying genes using JBrowse. It provides users with a means to build systems genetics models and generate hypotheses from large-scale population genomics data. We also substantially upgraded the EucGenIE resource and show how it enables users to combine genomics and systems genetics approaches to discover candidate genes involved in biotic stress responses and wood formation by focusing on two multigene families, laccases and peroxidases.ConclusionsqtlXplorer adds a new dimension, population genomics, to the EucGenIE and PlantGenIE environment. The resource will be of interest to researchers and molecular breeders working in Eucalyptus and other woody plant species. It provides an example of how systems genetics data can be integrated with functional genetics data to provide biological insight and formulate hypotheses. Importantly, integration within PlantGenIE enables novel comparative genomics analyses to be performed from population-scale data.

Embedding genetics experiments in restoration to guide plant choice for a degraded landscape with a changing climate

SummaryWe overview an array of common garden genetics trials established across multiple sites and embedded in restoration plantings in the harsh, modified Midlands region of the island of Tasmania, Australia. The steps in establishing the common gardens, the diversity of experimental designs employed and their objectives are described. These trials provide a long‐term framework to test species choices and provenancing strategies, as well as enhance our understanding of adaptive genetic variation within key eucalypt species used for ecological restoration. Early results signal genetic‐based differences in early‐age performance, and we showcase the multiple benefits of integrating research infrastructure into restoration plantings.

Expression Patterns and Regulation of Non-Coding RNAs during Synthesis of Cellulose in Eucalyptus grandis Hill

Cellulose, an essential structural component in the plant cell wall and a renewable biomass resource, plays a significant role in nature. Eucalyptus’s excellent timber tree species (including Eucalyptus grandis Hill) provide many raw materials for the paper and wood industries. The synthesis of cellulose is a very complex process involving multiple genes and regulated by various biological networks. However, research on regulating associated genes and non-coding RNAs during cellulose synthesis in E. grandis remains lacking. In this study, the wood anatomical characteristics and chemical indexes of E. grandis were analyzed by taking three different parts (diameter at breast height (DBH), middle and upper part of the trunk) from the main stem of E. grandis as raw materials. The role of non-coding RNAs (Long non-coding RNA, lncRNA; Micro RNA, miRNA; Circle RNA, circRNA) on regulating candidate genes was presented, and the network map of ceRNA (Competing endogenous RNA) regulation during wood cellulose biosynthesis of E. grandis was constructed. The transcriptome sequencing of nine samples obtained from the trunk of the immature xylem in E. grandis at DBH, middle and upper parts had a 95.81 G clean reading, 57,480 transcripts, 7365 lncRNAs, and 5180 circRNAs. Each sample had 172–306 known miRNAs and 1644–3508 new miRNAs. A total of 190 DE-lncRNAs (Differentially expressed long non-coding RNAs), 174 DE-miRNAs (Differentially expressed micro RNAs), and 270 DE-circRNAs (Differentially expressed circle RNAs) were obtained by comparing transcript expression levels. Four lncRNAs and nine miRNAs were screened out, and the ceRNA regulatory network was constructed. LncRNA1 and lncRNA4 regulated the genes responsible for cellulose synthesis in E. grandis, which were overexpressed in 84K (Populus Alba × Populus glandulosa) poplar. The cellulose and lignin content in lncRNA4-oe were significantly higher than wild type 84K poplar and lncRNA1-oe. The average plant height, middle and basal part of the stem diameter in lncRNA4-oe were significantly higher than the wild type. However, there was no significant difference between the growth of lncRNA1-oe and the wild type. Further studies are warranted to explore the molecular regulatory mechanism of cellulose biosynthesis in Eucalyptus species.

Фітопрепарати в комплексному лікуванні запальних захворювань пародонту

In this article the review of foreign and domestic literary sources, which are devoted to the actual problem of modern dentistry – the treatment of inflammatory diseases of periodontal tissues: gingivitis and periodontitis are presented. The complex approach to their treatment involves the appointment of a significant amount of pharmacotherapeutic drugs. Therapeutic failures and iatrogenic complications have led to the fact that today the interests of doctors and population to medicinal products significantly increased. The purpose of the study is to analyze the data of scientific literature on the use of plant-based medicinal products for the treatment of periodontal inflammatory diseases over the past 10 years. Materials and methods. Comprehensive and systematic analysis of literature. Review and discussion. The analysis of information sources on the use of plant-based medicinal products in dentistry both independently and in the composition of medical and prophylactic means has established that the modern assortment of plant-based preparations in the pharmaceutical market of Ukraine to a certain extent is limited. The emergence of new plant-based species that have been tested in conditions of experimental pathology and require an evidence-based clinical base is noted. The composition of plant-based preparations used for the treatment of inflammatory periodontal diseases include vitamins, biologically active substances, glycosides, alkaloids, in connection with a wide range of action: antiseptic, anti-inflammatory, regenerating, hemostatic, antioxidative. The data on plant-based preparations that are most often used such as chamomile extracts, calendula, hypericum, plantain, kalanchoe, aloe, eucalyptus, milfoil, nettle, calamus and plant-based species are summarized. The medicinal agents considered are mainly recommended for local treatment of periodontal diseases in the form of dental care means, mouth rinse, gel, chewing gum, herbal liquer. It is known that the complex treatment of periodontal diseases includes a general influence on the body. The properties of green tea with its wide range of actions are investigated. With antioxidant properties, it can be a healthy alternative for controlling destructive changes in periodontal diseases. Attention is drawn to the proposed unique natural complex “Resverazin” due to a wide range of pharmacological action, low toxicity and relative safety. The drug produces antioxidant, anti-inflammatory, immune stimulating, vasodilative, neuroprotective action. Conclusion. Based on the literature analysis, it can be concluded that the accumulated experimental and clinical data on the therapeutic properties of plants prove perspective of their use in the complex treatment of inflammatory periodontal diseases. Future studies are mandatory for further confirmation of the effectiveness of these medicinal plants

Anthocyanins, Phenolic Compounds, and Antioxidants from Extractions of Six Eucalyptus Species

The leaves of Eucalyptus have multiple biological activities such as antimicrobial, antiseptic, antioxidant, and antifungal. A Soxhlet extraction, SLE, and HD were used to obtain extracts from the leaves of six Eucalyptus species, E. globulus, E. oblicua, E. pavaflora, E. camaldulensis, E. viminalis, and E. nitens, and to study their antioxidant capacity. Solvents such as acetone, dichloromethane, ethanol, hexane, methanol, and water were used to study how polarity influences extraction yields. The SLE method achieved higher or similar yields, depending on the species and its composition, than the Soxlet method at a temperature of 50 °C. The highest yields were obtained with E. viminalis with methanol (42.5 wt.%), the highest phenolic content was obtained with E. nitens with methanol (124.17 mg GAE/g of extract), and the highest anthocyanin levels obtained were with E. nitens with hexane (5.05 mg CC/g of extract). E. nitens obtained almost five times more phenolic content than E. globulus; therefore, it is the most promising species. The high content of the compounds analysed confirm the good potential of these species to obtain value-added compounds. Our results demonstrate that the differences in the extract contents depend on the polarity of the solvents used. In addition, the use of these species will reduce the residue in the forest, which is greatly beneficial.

Adaptability and stability of novel eucalypt species and provenances across environments in Brazil at two assessment

Adaptability and stability of novel eucalypt species and provenances across environments in Brazil at two assessment

PENGARUH POLA TANAM TERHADAP PERTUMBUHAN TANAMAN REHABILITASI HUTAN DAN LAHAN DI RPH TAROGONG BKPH LELES KPH GARUT

ABSTRACTThis study aims to determine whether there is an effect of cropping plants on the growth of pine (pinus merkusii), eucalyptus (eucaliptus alba), puspa (schima wallichii) and avocado species (parsea americana) on RHL activities in RPH Tarogong, BKPH Leles, KPH Garut, West Java and Banten regional divisions. This study was conduct in three series. First, determining the location to be measure second, determination of the measuring plot. Third, making plant measuring by size 40x25m with an area 0,1ha by using systematic sampling with random start metode. Primary data retrieval, namely from the results of the making of the measuring plots according to the plan, then recorded in the taly sheet and then processed according to the parameters determined in the study. Data analysis was conduct to determine the role of each cropping pattern on diameter. The height and robustness of the plants were analyze using the independent sample t-test of the unpaired independent variables. Thus, we can formulate a general definition that the independent sample t is a statistical analysis that aims to compare two unpaired samples. Based on the results of study and discussion, it can be concluded that the growth of eucalyptus (Eucaliptus Alba) and Puspa plant spesies (Schima Wallichii) is better in the agroforestry pattern than in the non-agroforestry pattern. The growth of pine (Pinus Merkusii) and avocado plants (Parsea Americana) is not affect by cropping patterns, so these types can be plant in both agroforestry and non-agroforestry cropping patterns.