Ethylenediaminetetraacetic Acid Group Research Articles

The comparison of platelet parameters in Platelet Rich Plasma (PRP) using Ethylenediaminetetraacetic Acid (EDTA) and Acid Citrate Dextrose (ACD) anticoagulants at Dr. Wahidin Sudirohusodo Hospital, Makassar, Indonesia

Background: Platelet Rich Plasma (PRP) is an autologous plasma with a platelet concentration above normal values. It contains high levels of growth factor, which plays an important role in tissue repair. The quality of PRP products is influenced by the platelet count, anticoagulants, speed, and centrifugation duration. This study aimed to determine the effectiveness of Ethylenediaminetetraacetic Acid (EDTA) and Acid Citrate Dextrose (ACD) in PRP preparation by measuring the platelet count (PLT) and platelet index (MPV and PDW).Methods: Experimental laboratory study involved 25 volunteers performed at Hasanuddin University Hospital from September-October 2020. The values of PLT, MPV, PDW were measured using Sysmex XS-800i®. The parameters comparison between EDTA and ACD groups was analyzed using an independent T-test. A comparison of pre and post centrifugation was using paired T-test (significant if p<0.05). Data were analyzed using SPSS version 22 for Windows.Results: The mean PLT in the EDTA sample was significantly lower than ACD (539.10x103/µL vs. 769.8x103/µL; p<0.05). Mean MPV and PDW in EDTA were significantly higher than ACD (10.7fL vs 8.9fL and 12.7fL vs 9,8fL; p<0,001). However, the mean MPV and PDW in the ACD sample did not change significantly (p>0.05).Conclusion: PRP with ACD had a higher platelet count than EDTA, without significant platelet index changes.ACD is a more effective anticoagulant for the preparation of PRP than EDTA.

Synthesis of Ethylenediaminetetraacetic Acid-Functionalized Chitosan Cryogels as Potential Sorbents of Heavy Metal Ions

An original functionalization strategy is proposed here to design chitosan (CS)-based cryogels with ethylenediaminetetraacetic acid (EDTA) moieties. Cryogels with aligned micro-sized tubular structures were further engineered through an unidirectional freezing approach. Attachment of EDTA groups onto CS chains was proved by 1H-RMN and FT-IR spectroscopy. The formation of EDTA-functionalized 3D porous CS-based cryogels was demonstrated by several methods of characterization (FTIR spectroscopy, optical microscopy, SEM, porosity measurements, swelling behavior, copper (II) retention capacity). The sorption tests pointed out the high potential of EDTA-functionalized CS-based cryogels for heavy metal ions retention.

EDTA detoxifies heavy metals on exposed beluga ( Huso huso ) with pollution stress: Growth performance, immunohaematology, blood biochemistry and antioxidant activity

Ethylenediaminetetraacetic acid (EDTA) is one of the most popular chelating agents. This study was conducted to investigate the effect of EDTA on the growth and health physiology of beluga (38.47 ± 0.58 g) before and after heavy metal stress (HMS). Five experimental diets, including control, 0.5EDTA, 1EDTA, 1.5EDTA and 2EDTA, were formulated to feed fish for eight weeks. The results showed that fish was fed with dietary 1.5EDTA (108.37 g) had a higher weight gain as compared with control (p < 0.05). Blood performance in the control group after HMS decreased but not in fish fed supplemented diets with EDTA. EDTA reduced uric acid and creatinine levels in beluga. Fish fed dietary 1.5EDTA had a higher value of blood performance (55.17), glucose (50.3 mg/dl), superoxide dismutase (SOD U/ml) (75.8) and glutathione peroxidase (234.0 U/ml) after HMS as compared with the control. Additionally, lower values of uric acid (3.05 mg/dl), creatinine (0.27 mg/dl) and AST (61.47 U/ml) after HMS in the 1.5EDTA group were observed (p < 0.05). Correlation analysis revealed a strong positive correlation between EDTA level and protein efficiency, lipid efficiency, and blood performance and a strong negative correlation with some parameters after stress, including SOD, glucose, uric acid, creatinine and aspartate transaminase (p < 0.01). In conclusion, feeding fish with the 1.5EDTA diet improved growth and detoxified heavy metals.

The Smear Layer Removal Efficiency of Different Concentrations of EDTA in primary teeth: A SEM Study

Objectives: The present study aims to evaluate the effects of different concentrations of ethylenediamine tetraacetic acid (EDTA) on smear layer removal in primary teeth by using scanning electron microscopy (SEM). Materials and Methods: The present study was performed with 28 extracted upper primary incisors assigned into four main groups (n=7) as 5%, 10% and 17% EDTA, and 1% sodium hypochlorite (NaOCl). The root canal surfaces (coronal, middle and apical) were scanned by SEM and scores of smear layer removal were recorded and compared after the root canal irrigation procedures. The results were analyzed using Kruskal–Wallis, Friedman and Dunn tests. Results: In all the root portions, although EDTA groups removed the smear layer more effectively than NaOCl, a statistically significant difference was observed between 17% EDTA and 1% NaOCl only in middle third (p &amp;lt;0.05). Also, smear layer was removed more effectively in coronal than apical in most of the groups (10% EDTA, 17% EDTA and 1% NaOCl) (p &amp;lt;0.05). Erosive defects were mostly seen in 17% EDTA and less than 10% EDTA group. These findings were not detected in 5% EDTA and 1% NaOCl group. Conclusions: It is possible to recommend the use of 5% EDTA irrigation solution in root canal treatment of primary teeth due to its similar smear layer removal efficacy with NaOCl and high concentration EDTA groups, low erosive potential and low concentration for periapical safety.

Investigation of the effect of different chelation solutions on penetration of resin-based and bioceramic sealers with a novel method.

This study aimed to investigate the effects of different chelation solutions on the penetration of resin-based and bioceramic root canal sealers into dentinal tubules using a device that assembles conventional microplate detection with automated digital microscopy. Eighty-four single-rooted teeth were prepared with 30.07 nickel-titanium instruments and divided into four groups of 21 according to the final irrigation solutions used: saline (control), ethylenediaminetetraacetic acid (EDTA), maleic acid (MA), and etidronic acid (HEBP). The samples were obturated with AH Plus, MTA Fillapex, or EndoSequence BC Sealer using the single-cone technique. The roots were then sectioned horizontally at 2, 6, and 10 mm and examined using a Cytation 5 reader and Gen5 software. Data were compared using two-way analysis of variance followed by univariate analyses and Tukey's multiple comparison test (p < .05). The mean penetration depth in the apical thirds was higher in the MA group than in the other groups (p =.013). The mean depth in the middle and coronal thirds was almost equal in the EDTA and HEBP groups. The mean depth was significantly higher in the coronal thirds of the root canals than in any other section in all final irrigation solution groups (p <.001). EndoSequence BC Sealer displayed higher penetration depth than AH Plus and MTA Fillapex, although the differences between the examined sealers were not statistically significant. The chelation solutions increased root canal sealer penetration into the dentinal tubule.

EVALUATION OF ALIZARIN DYE PENETRATION INTO DENTINAL TUBULES FOLLOWING FINAL RINSE BY Q-MIX AND APPLE VINEGAR: AN IN VITRO STUDY

Introduction: Successful treatment of a root canal aims for removal of microorganisms from the canal. Therefore, intraradicular removal of the smear layer is a remarkable measure for long-established endodontic success. Aim of the study: to assess Alizarin dye penetration into dentinal tubules following Q-Mix, apple vinegar, and 17% ethylenediaminetetraacetic acid (EDTA) under confocal laser scanning microscope (CLSM). Materials and method: Thirty extracted single-canaled mandibular premolars went through decoronating to 15 mm length to undergo cleaning and instrumentation. Then, they were allocated at random into three groups based on the final rinse used: Group I: 10 ml of 17% EDTA for 1 minute, Group II: 10 ml of apple vinegar for 1 minute, and Group III: 10 ml Q-MIX for 1 minute. All root canals were irrigated by 5ml Sodium hypochlorite labeled with Alizarin red. Samples were horizontally sectioned and evaluated under CLSM at distinct canal levels. Data were analyzed using (Kruskal Wallis and Friedman’s) Tests. Results: The three tested groups showed no statistically significant difference with p> 0.05. Regarding the coronal and middle thirds of EDTA, ACV, and Q-Mix groups, most of the specimens recorded score 3 (less than 50% of the whole number of dentinal tubules were penetrated with the dye) while the specimens of apical thirds recorded score 2 (traces of the dye could be seen along the internal canal surface). Conclusions: Q-Mix, Apple vinegar, and EDTA promoted the penetration through dentinal tubules in the coronal and middle sections superior to the apical section of the root canal.

Efficacy of SmearOFF, maleic acid, and ethylenediaminetetraacetic acid combined with sodium hypochlorite in removal of smear layer from curved root canals: In vitro study

Introduction: Mechanical instrumentation of the root canal system produces an amorphous smear layer. The aim of the present research was to assess the effect of SmearOFF, 7% maleic acid (MA) and 17% ethylenediaminetetraacetic acid (EDTA) when combined with sodium hypochlorite (NaOCl) in removal of smear layer from curved root canals. Materials and Methods: Forty mandibular molars were selected of which only the mesial roots were prepared biomechanically and categorized into four equal groups on the basis of the irrigation protocol: (1) SmearOFF, (2) 7% MA, (3) 17% EDTA, and (4) 0.9% saline. Teeth were then decoronated; mesial roots were cleaved lengthwise and scanning electron microscopic analysis was performed for evaluation of existence of smear layer. Results: On intergroup comparison, there has been found to be notable dissimilarity among the SmearOFF and MA groups in coronal, middle and apical thirds of the root canal system (P > 0.05). However, in EDTA group, there was no notable difference in coronal and middle third. However, in apical one-third, smear layer was cleared less effectively when compared to SmearOFF and MA (P Conclusion: In combination with NaOCl, SmearOFF, and MA had superlative smear layer eradication efficacy in contrast to EDTA in apical third of the curved root canals.

Smear Layer Removal from the Apical Third Using the Er,Cr:YSGG Photon-induced Photoacoustic Streaming.

The purpose was to study the influence of Erbium,Chromium:Yttrium Scandium Gallium Garnet (Er,Cr:YSGG) laser at short pulse duration on smear layer removal from apical root third. Twenty-four single-rooted mandibular premolars were used. The roots length was cut to 14 mm and instrumented using the Protaper Gold system to size 40/0.06. The samples were divided into four groups (n=6) as follows: (A) conventional irrigation with 5 mL of 17% Ethylenediaminetetraacetic acid ( EDTA), (B) passive ultrasonic irrigation with 5mL of 17% EDTA, (C) Er,Cr:YSGG induced photoacoustic streaming (PIPS) with 5 mL of 17% EDTA and (D)Er,Cr: YSGG induced PIPS with 5 mL of 5.25% sodium hypochlorite (NaOCl). After final irrigation with normal saline and drying, the roots were painted with nail varnish externally, and 2%methylene blue dye was injected into the canal. The tooth split horizontally at the fourth millimetres. The picture was taken by a professional Digital SLR camera. The dye penetration was measured using analytical software (measure picture CAD-KAS Kessler Germany). Dunnett's test was used to compare control group against experimental groups. After the statistical test, the highest mean percentage was presented in the laser group with 17% EDTA (Group C=85.9804) followed by the laser group with 5.25% NaOCl (Group D=69.7817) and ultrasonic group (Group B=34.5453) respectively and the lowest mean percentage was in the control group (Group A=20.7969). Based on this in vitro study, PIPS technique using Er,Cr:YSGG pulse laser (0.5 W,5 Hz, air and water off) at short pulse duration and 17% EDTA proved effectively in smear layer removal, while the PIPS proved an acceptable result when the laser is used alone assisted by NaOCl.

Effect of EDTA and hyaluronic acid on the activity of periodontal ligament fibroblasts and the expression of cytokines

Purpose To investigate the effect of ethylenediaminetetraacetic acid (EDTA) and hyaluronic acid (HA) on the activity of periodontal ligament fibroblasts (PDLFs) and the expression of cytokines. Methods Twelve wisdom teeth extracted due to orthodontics treatment were selected and prepared into 5 mm×4 mm root pieces, then divided into EDTA group, HA group, EDTA+HA group and untreated group according to different treatment methods. They were placed in the well plate and PDLFs inoculated on the root piece. After 24 and 48 h of inoculation, the cell proliferation of each group was detected by MTT assay. The adhesion of PDLFs was observed under microscope. The inflammatory cytokines (IL-6, IL-8 and IL-1β and TNF-α) levels produced by PDLFs were determined by ELLISA and Western Blot. SPSS 22.0 software package was used for statistical analysis. Results After 24 h and 48 h inoculation, all the treatments promoted cell proliferation and adhesion of PDLFs compared with the untreated group, and the combined treatment promoted cell proliferation and adhesion significantly better than single treatment (P 0.05). The results of ELLISA and Western blot showed that compared with the untreated group, the treatment groups inhibited the expression of inflammatory factors IL-6, IL-1β and TNF-α, and promoted the expression of IL-8, and the effect of EDTA+HA group was much more significant(P Conclusions EDTA+HA can significantly promote the growth of periodontal ligament fibroblasts and inhibit the expression of inflammatory cytokines, which may be related to its ability to enhance the adhesion of fibroblasts and then improve their viability.

Evaluation of 2% Chlorhexidine and 2% Sodium Fluoride as Endodontic Irrigating Solutions on Root Dentine Microhardness: An In Vitro Study.

Objectives The aim of this study was to evaluate the effects of 2% chlorhexidine gluconate (CHG) and 2% sodium fluoride (NaF) as endodontic irrigants on microhardness of root dentin. Materials and Methods In this in vitro study, access cavity and root canal preparations were done on 24 freshly extracted anterior teeth. After sectioning into 24 dentin discs using hard tissue microtome in 2 mm thickness, all samples were immersed in solutions of 17% ethylenediamine tetra-acetic acid (EDTA) (2 minutes) followed by 2.5% of sodium hypochlorite (NaOCl) (10 minutes). Then samples were randomly divided into three groups based on the irrigant used: Group I: saline (control group); Group II: 2% NaF; Group III: 2% CHG for two minutes each. Dentin microhardness was measured before (pretreatment), during (after treatment with 17% EDTA and 2.5% NaOCl), and after the experimental period (after treatment with saline, 2% NaF, and 2% CHG) using a Vickers indenter. Statistical evaluation of the data was done using one-way analysis of variance (ANOVA) and the Student’s t -test, and the values are tabulated. Results Specimens rinsed in 2% CHG showed a significant increase in Vickers hardness number (VHN) values ( p < 0.05), as compared with EDTA and NaOCl groups, whereas saline and 2% NaF groups showed no significant difference. Conclusions NaF did not show any significant effect on microhardness of the root dentin. CHG as an irrigant was seen to have a strengthening effect on dentin microhardness in comparison to NaOCl and EDTA, which has decreased the strength of root dentin.

LPCGF and EDTA conditioning of the root surface promotes the adhesion, growth, migration and differentiation of periodontal ligament cells.

Liquid-phase concentrated growth factor (LPCGF), a new-generation platelet concentrate, may potently stimulate human periodontal ligament (PDL) cells. This study assessed the effectiveness of ethylenediaminetetraacetic acid (EDTA) and/or LPCGF on periodontally diseased root surfaces through their effects on PDL cells. Dentin blocks prepared from periodontal teeth were divided into four groups and treated as follows: group I, scaling and root planning (SRP); group II, SRP + EDTA; group III, SRP + LPCGF; and group IV, SRP+EDTA + LPCGF. PDL cells were cultured on dentin blocks, and LPCGF-induced biological effects were evaluated by migration and cell adhesion/proliferation assays. Furthermore, PDL cell differentiation was assessed by real-time polymerase chain reaction (PCR). Significantly more adherent cells were observed in the EDTA, LPCGF and combination treatment groups than in the control group. Root conditioning with EDTA and/or LPCGF enhanced cell proliferation and migration more than SRP did. Compared with the control group, the combined treatment group exhibited significant upregulation of cell differentiation-related genes. Electron microscopy of the tooth surface revealed removal of the smear layer and exposed dentin holes in the EDTA-treated group but not in the control group. EDTA and LPCGF application to periodontitis-affected root surfaces forms a surface suitable for cell attachment, growth, migration and differentiation. Thus, LPCGF is promising in clinical periodontics applications. Further studies to support these results are necessary.

Effect of a new irrigant solution containing glycolic acid on smear layer removal and chemical/mechanical properties of dentin

The objective of this study was to evaluate the effects of glycolic acid (GA) (with pH 1.2 and 5) and ethylenediaminetetraacetic acid (EDTA) on the chemical and mechanical properties of dentin to investigate the potential use of GA as final irrigant in the root canal therapy. Specifically, changes in microhardness, smear layer removal, erosion, mineral content distribution, apatite/collagen ratio and flexural strength of mineralized dentin treated with GA were assessed. Saline solution was used as a negative control. Knoop microhardness (KHN) was measured on the root canal lumen of root segments. Dentin beams were used for 3-point flexural strength (σ) test. Scanning electron microscopy (SEM) images of root sections were obtained for evaluation of smear layer removal and dentin erosion on root segments and energy dispersive X-ray spectroscopy (EDS) was used for mineral content distribution. The apatite/collagen ratio (A/C) in dentin powder were examined by Fourier transform infrared (FTIR) spectroscopy. KHN, σ and A/C results were statistically analyzed with ANOVA and Tukey tests (α = 0.05). Smear layer and dentin erosion scores were analyzed with Kruskal-Wallis and Dunn tests (α = 0.05). Root dentin treated with EDTA and GA presented similar KHN regardless of the pH (p > 0.05). However, KHN was significantly reduced in EDTA and GA groups when compared to control group (p<0.001). GA showed the same ability to remove the smear layer and to cause dentin erosion as EDTA. EDS results showed that the GA and EDTA solutions did not alter the dentin mineral content distribution. The apatite/collagen ratio reduced with all irrigant solution and was the lowest with GA pH 5 (p<0.001), while σ was not significantly affected by the experimental solutions (p = 0.559). It can be concluded that GA has similar ability to remove the smear layer than EDTA. GA does not affect negatively the chemical/mechanical properties and it does not increase dentin erosion. The use of GA with low pH seems to promote less change in collagen/apatite ratio, but further studies are needed to establish an ideal clinical protocol. Therefore, this study supports the potential use of GA as an alternative final irrigation solution for root canal preparation.

Comparative evaluation of two chelating agents in collagen fiber network modification over dentinal tubules: An in vitro analysis

ObjectiveTo compare effectiveness of ethylenediaminetetraacetic acid (EDTA) and citric acid in removing collagen fiber network covering dentinal tubules of human teeth. Materials and MethodsEighteen dentin discs were divided in three groups; Gp 1: discs received no treatment (control), Gp 2: discs etched with 17% EDTA (pH = 7.1), and Gp 3: discs etched with 6 wt% citric acid (pH = 4.0). Scanning electron microscopy (SEM) was performed to assess collagen fiber removal and X-ray diffraction (XRD) was implemented to analyse crystal peaks of discs. ResultsThe SEM analysis demonstrated more collagen removal with EDTA treatment compared to citric acid treated specimens. Grade 6 (81% to 100% fiber removal) was mostly achieved for Gp 2 samples whereas grade 2 (1% to 20% fiber removal) was mostly achieved for Gp 3 samples and inter-group comparisons between these groups were statistically significant (p < 0.05). X-ray diffractogram of control and experimental samples demonstrated absence of calcite phase in experimental groups. The change in peak shapes and intensities were observed and citric acid treated samples revealed more intense peaks than EDTA group. ConclusionOur study found 17% EDTA to be more effective in removing collagen fibers when matched with 6% citric acid.

Effect of root conditioning agents hyaluronic acid, EDTA and chlorhexidine on the attachment of human gingival fibroblasts to healthy root surface

BackgroundThe gold standard treatment of periodontal diseases is scaling and root planing (SRP). Different adjunctive root conditioning agents such as hyaluronic acid (HA), ethylenediaminetetraacetic acid(EDTA), and chlorhexidine (CHX) have been used with SRP to improve the smear layer removal and the healing of periodontal tissues. The aim of this study was to compare the effect of manual scaling with or without HA, EDTA, or CHX root conditioning on the attachment and viability of human gingival fibroblasts (GF). MethodsFifteen healthy single rooted teeth were co llected and divided randomly into a scaled (n = 12) and non-scaled control group (n = 3). The scaled roots were subdivided equally into four groups; the first group did not receive any chemical treatment, while the remaining groups were treated with the conditioning agents HA or 17% EDTA or 0.2% CHX gel. Gingival fibroblasts were seeded on the top of each root and incubated for 48 h to allow attachment to the roots. The viability of fibroblasts attached to the root surface was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. ResultsThe cell viability was the highest in the scaled only group (p = 0.0001) while the lowest was in the scaled with EDTA group (p > 0.05). The scaled group was the highest followed by the HA and CHX groups, while the EDTA group showed the lowest mean value. ConclusionSRP remains the superior method for regaining cell attachment to the root surface, leading to better periodontal health, and adjunctive therapies did not enhance the GF attachment to the root surface beyond the effect of SRP. Further studies are needed to investigate the effect of root conditioning agents on periodontally diseased teeth in vitro and compare them in vivo.

Amphiphilic ligand modified gold nanocarriers to amplify lanthanide loading for ultrasensitive DELFIA detection of Cronobacter.

Conventional dissociation-enhanced lanthanide (Ln3+) fluoroimmunoassays (DELFIAs) using Ln3+ chelate-labeled antibodies as molecular probes exhibit limited sensitivity because of their relatively low Ln3+ labeling ratio per biomolecule. Herein, we applied gold nanoflowers (AuNFs) as amplified nanocarriers to increase the Ln3+ labeling ratio in a single molecular binding event for improving the sensitivity of traditional DELFIA. Two thiolated amphiphilic ligands (thiolated ethylenediaminetetraacetic acid (EDTA) and thiolated acylhydrazine-terminated ligands), consisting of a hydrophobic alkane chain, oligo(ethylene glycol) unit, and functional terminal of the EDTA or acylhydrazine group, were designed for the surface modification of AuNFs. The resultant ligand-coated AuNFs exhibited dual functions of Ln3+ chelation via the EDTA group and oriented attachment of antibodies via the acylhydrazine group. By utilizing 80 nm AuNFs as amplified carriers, we demonstrated that the maximum Eu3+ loading amount reached 1.07 × 104 Eu3+ ions per AuNF, which is approximately two to three orders of magnitude higher than that of traditional molecular probes, thereby amplifying the luminescence signal and enhancing the sensitivity of DELFIA. By combining a magnetic-mediated sandwich-type DELFIA method, the designed amplified AuNF nanoprobes achieved an ultrasensitive luminescence detection of Cronobacter muytjensii with a limit of detection (LOD) of 1.2 × 102 cfu mL-1 in a powdered infant formula. This LOD value was ca. 230-fold lower than that of the traditional colorimetric immunoassay. The designed signal amplification strategy using bifunctional ligand-modified AuNFs as enhanced Ln3+ nanocarriers provided a huge potential for building various ultrasensitive luminescence immunoassays for in vitro biodetection.

Smear Layer Removal from Root Canal Dentin and Antimicrobial Effect of Citric Acid-modified Chlorhexidine.

Objective:To study the effectiveness of various concentrations of citric acid (CA) added to 2% chlorhexidine (CHX) on smear layer removal from the root canal wall and antimicrobial efficacy against Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans).Methods:Fifty-three single-rooted mandibular premolars were decoronate and the root canals underwent mechanical instrumentation using MTwo rotary files to size 40/0.06. The samples were then randomly divided into 5 groups according to the root canal irrigants to be used: 17% ethylenediaminetetraacetic acid (EDTA), 2% CHX, 1%, 6%, and 10% citric acid-modified 2% chlorhexidine (CAmCHX). Three teeth irrigated with phosphate-buffered saline (PBS) were used as a negative control. The smear layer removal effectiveness was evaluated under scanning electron microscopy (SEM). Images were randomly taken at the apical, middle, and coronal third level. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney U tests. Disc diffusion and direct exposure tests were performed along with three additional control groups consisting of 1%, 6%, and 10% CA groups to assess and compare the antimicrobial efficacy of irrigants against E. faecalis and C. albicans. Statistical analysis was conducted using one-way ANOVA and Dunnett’s T3 tests.Results:Smear layer removal effectiveness in 17% EDTA group and 6% and 10% CAmCHX groups were not significantly different in the coronal and apical third of the root canal (P>0.05), however at the middle third of the root canal, the 10% CAmCHX group had significantly less remaining smear layer than all of the other experimental groups (P<0.05). There was significantly more smear layer remnant in the CHX group (P<0.01). For antimicrobial efficacy, the largest growth inhibition zone against E. faecalis was recorded in the 10% CAmCHX group (P<0.05). For planktonic E. faecalis, 1%, 6%, and 10% CAmCHX demonstrated an insignificant difference in antimicrobial efficacy compared to CHX (P>0.05). CA demonstrated no antifungal effect against C. albicans. Whereas, 6% and 10% CAmCHX resulted in the largest growth inhibition zone. Also, adding CA to CHX resulted in an insignificant difference in antifungal effect against planktonic C. albicans compared to CHX (P>0.05).Conclusion:When CA was added into CHX, the mixed irrigant demonstrated smear layer removal ability. Additionally, its antimicrobial effect remained the same.

Effect of different chelating agents on bovine tissue dissolving capacity of sodium hypochlorite

Background: The success of endodontic therapy depends on thorough cleaning of the canal system. This is done by the use of various irrigants together with mechanical cleaning. The most popular irrigant used is sodium hypochlorite (NaOCl) in different concentrations. It has an added advantage in that it is a tissue dissolvent. To improve the removal of the smear layer in the canal, NaOCl is used with chelating agents such as ethylenediaminetetraacetic acid (EDTA), etidronic acid, or chitosan. The aim of this study was to evaluate the effect of different chelating agents when used with NaOCl on tissue dissolving capability. Materials and Methods: Cleaned bovine tissue was cut into equal pieces of 4 mm × 4 mm × 2 mm and divided into 60 samples having a weight of 70 ± 3 mg. Irrigant solutions were divided into four groups. Group 1: 3% NaOCl, Gp 2: 3%NaOCl + 17% EDTA, Group 3: 3% NaOCl + 18% Etidronic acid, and Group 4: 3% NaOCl + 0.2% chitosan in acetic acid. Samples were taken into test tubes and kept in 10 ml solution of each group for 10 min. Later, samples were taken out from solution and washed with distilled water, blotted dry, and weighed again for change of weight. The difference between the initial and final weights was used to calculate the weight loss. Results: There was a significant difference between the sample weight before and after treatment for all the groups. NaOCl (Group 1) showed best percentage difference of weight loss, followed by NaOCl + Etidronic acid (P Conclusion: Etidronic acid has shown better dissolution capacity as compared to EDTA and chitosan group when used with NaOCl.

Smear Layer Removal and Microhardness Alteration Potential of a Naturally Occurring Antioxidant - An In Vitro Study.

Introduction: It is well-known in the field of endodontics that the presence of a smear layer in the root canals can harbor bacteria and limit the penetration of irrigating solutions and intracanal medications into the dentinal tubules which, in turn, causes the failure of endodontic treatment. Removing the entire smear layer throughout the root canal is essential for the success of endodontic treatment and these chemical agents that facilitate the removal of the smear layer are called chelating agents. Ethylenediaminetetraacetic acid (EDTA), being the most widely used chelating agent, brings about increased reduction in the microhardness of the root dentin, thereby making it friable.N-acetyl cysteine (NAC) is naturally occurring antioxidant that has various beneficial properties for the human being. Several studies have been done in determining the antimicrobial efficacy of NAC against various endodontic pathogens and concluded NAC to be advantageous. The chelating property of NAC has been utilized in heavy metal detoxification, where it binds to the metal ion and removes them from the human system. However, this chelating property has not been explored in the field of endodontics.Aim: This study was aimed to compare the ability of N-acetyl cysteine with the conventional chelating agent in the removal of the smear layer and in altering the microhardness of root dentin.Materials and Methodology: A total of 84 single-rooted human mandibular premolars with relatively similar dimension and morphology, freshly extracted with closed apices, were collected from adult patients. The crowns of all specimens were cut transversally at the cementoenamel junction (CEJ) with the double-faced diamond disc at low speed, with water coolant, to obtain a 12 mm root length. The root canals were randomly divided into three equal groups according to the final irrigation solutions: Group I: 17% EDTA, Group II: 20% NAC, and Group III: distilled water (control). They were then randomly divided into two parts: scanning electron microscope (SEM) analysis for the extent of smear layer removal and microhardness evaluation using the Vicker’s hardness test.Results: The smear layer removal ability of EDTA and NAC were more effective in the coronal and middle thirds of the root canal. However, both groups showed less smear layer removal in the apical region. Specimens treated with distilled water showed the least reduction in the smear layer throughout the length of the root canals. Regarding the evaluation of microhardness, both EDTA and NAC had a significant reduction in root dentin microhardness. However, the percentage of dentin microhardness reduction was significantly more in the EDTA group (p < 0.05) than N-acetyl cysteine in the coronal, middle, and apical third of the root canals.Conclusion: The chelating property of NAC is equally effective to that of EDTA in the smear layer from the root canal, and it induced a significantly lesser reduction in microhardness of root dentin than EDTA.

Therapeutic effect of ethylenediaminetetraacetic acid irrigation solution against wound infection with drug-resistant bacteria in a rat model: an animal study.

ObjectivesIrrigation is the cornerstone of treating skeletal infection by eliminating pathogens in wounds. A previous study shows that irrigation with normal saline (0.9%) and ethylenediaminetetraacetic acid (EDTA) could improve the removal of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) compared with normal saline (NS) alone. However, it is still unclear whether EDTA solution is effective against infection with drug-resistant bacteria.MethodsWe established three wound infection models (skin defect, bone-exposed, implant-exposed) by inoculating the wounds with a variety of representative drug-resistant bacteria including methicillin-resistant S. aureus (MRSA), extended spectrum beta-lactamase-producing E. coli (ESBL-EC), multidrug-resistant Pseudomonas aeruginosa (MRPA), vancomycin-resistant Enterococcus (VRE), multidrug-resistant Acinetobacter baumannii (MRAB), multidrug-resistant Enterobacter (MRE), and multidrug-resistant Proteus mirabilis (MRPM). Irrigation and debridement were repeated until the wound culture became negative. The operating times required to eliminate pathogens in wounds were compared through survival analysis.ResultsCompared with other groups (NS, castile soap, benzalkonium chloride, and bacitracin), the EDTA group required fewer debridement and irrigation operations to achieve pathogen eradication in all three models of wound infection.ConclusionIrrigation with EDTA solution was more effective than the other irrigation fluids used in the treatment of wound infections caused by drug-resistant pathogens.Cite this article: Z. Deng, F. Liu, C. Li. Therapeutic effect of ethylenediaminetetraacetic acid irrigation solution against wound infection with drug-resistant bacteria in a rat model: an animal study. Bone Joint Res 2019;8:189–198. DOI: 10.1302/2046-3758.85.BJR-2018-0280.R3.

The effect varied scanning electron microscopy desiccation techniques has on demineralized dentin

The study objective was to assess (a) the effect of a rubbing-application of ethylenediaminetetraacetic acid (EDTA) or citric acid (CA) has on the ultrastructure of surface dentin and (b) the effect of two scanning electron microscopy (SEM) desiccation preparation techniques have on the collagen surface produced. Treatment regions on proximal root surfaces of extracted human teeth were root planned to expose dentin. Cotton pellets soaked in either 30% CA or 24% EDTA solution were rubbed on the treatment region then processed for SEM using one of two desiccation techniques, that is, (a) critically point dried from liquid CO2 (control) or (b) air-dried from tetramethylsilane (experimental). Specimens were coated with gold/palladium and viewed/photographed with an SEM. Specimens of the control groups displayed tufted fibrils (CA > EDTA) with many dentin tubules being partially obscured by overhanging fibrils. Air-dried specimens of both treatment groups displayed a flat intact monolayer devoid of a matted meshwork of fibrous collagen. Discrete fibril "sprigs," emanating from the surface monolayer, were characteristic of the EDTA group only. The rubbing-application of EDTA on dentin produces a tufted fibril surface somewhat similar to that produced by CA. Air-drying desiccation of both resulted in marked distortion with fibril collapse/coalescence of the tufted collagen matrix.