Combustion instability has arisen as a substantial defect for power generating systems. This is caused by the acoustic sources of the combustor, such as indirect noise, produced by accelerating hot spots, which are less explored due to their complex nature. Understanding the physics of the hot spots, therefore, is of crucial importance to reduce the noise and subsequent instabilities. Further, hot spots are a difficulty in internal combustion engines motivated auto ignition. In this study, hot spot behavior in a combustor of lean-premixed flame of Ethylene, a type of biofuel, influenced by hydrodynamic and thermal conditions, is numerically studied using the flamelet model and large eddy simulation. The impacts of inlet turbulence intensity and preheating unburnt mixture on hot spots are examined in a thermally convective and adiabatic combustor. The results show that although preheating inlet mixture improves combustion efficiency, it aids the hot spot's survival (more than 40 percent improvement in wave’s dissipation), and may cause significant instabilities. Similar to increasing the unburnt mixture temperature, decreasing the turbulence intensity and the excitation amplitude diminishes dissipation and dispersion of the wave, which makes the combustor more prone to probable instabilities. Amongst the studied parameters, the turbulence intensity and convective cooling on the walls are the most effective in decreasing the wave’s strength by approximately 85 and 95 percent, respectively. It is indicated that a hot spot in real conditions of a combustor is prone to a high level of annihilation.