Ethylenediaminetetraacetic Acid Solution Research Articles

Comparison and evaluation of two reciprocating root canal instruments on removal of smear layer by using two irrigants at apical one-third of the root canal-an ex vivo-scanning electron microscopic study.

Aim:To compare and evaluate the cleaning ability of two reciprocating systems, that is, WaveOne (WO) and self-adjusting files (SAF) in terms of removal of smear layer using sodium hypochlorite (NaOCl) and chlorhexidine gluconate (CHX) at apical one-third of the root canal.Materials and Methods:Forty-three human maxillary anterior teeth were selected. The canal was instrumented manually up to a number 25 K-file size. WO and SAF were used to prepare the root canals. The following groups were studied: Group 1 using WO:-Group 1a = 5% NaOCl + 17% ethylenediaminetetraacetic acid (EDTA), Group 1b = 2% CHX + 17% EDTA, Group 2 using SAF:-Group 2a = 5% NaOCl + 17% EDTA, Group 2b = 2% CHX + 17% EDTA. All roots were split longitudinally and subjected to scanning electron microscopic. The presence of smear layer apical thirds was evaluated using a 5-score evaluation system. Chi-square tests were used for statistical analysis.Results:The results showed no significant difference in smear layer removal among both reciprocating system and among the irrigants. Group 2a exhibited the best results and was close to significant difference in score (P > 0.05). The NaOCl + EDTA solution used in Group 1a and Group 2a could effectively remove the smear layer in most of the specimens. However, SAF was more effective than WO in the apical third of the canal.Conclusion:The results obtained from the present study suggest that using the SAF system and continuous irrigation action with NaOCl and EDTA solution could overcome the difficulty of removing smear layer even in hard-to-reach regions of the root canal.

Optimum immunohistochemical procedures for analysis of macrophages in human and mouse formalin fixed paraffin-embedded tissue samples.

Macrophages are closely related to various diseases and it is therefore important that the properties of macrophages are adequately evaluated in human diseases and mouse disease models. Immunohistochemistry (IHC) of formalin fixed paraffin-embedded (FFPE) samples is a very useful tool for examination of macrophages; however, an adequate IHC protocol is required for the examination of macrophage states. In this study, we assessed various antigen retrieval methods in order to devise the optimal protocols for staining of macrophages with a range of antibodies. Optimum combinations of primary antibodies and antigen retrieval protocols were determined; for example, heat treatment with ethylenediamine tetraacetic acid solution, pH 8.0, was the best procedure for IHC using mouse anti-Iba1 and human anti-CD11b, -CD163, -CD169, -CD204, and -CD206 antibodies. Moreover, we found that the immunoreactivity of sliced tissue sections decreased gradually over time in long term storage but that this immunoreactivity was preserved in storage at -80 °C in a deep freezer. The optimal IHC protocols and storage procedures that were determined in this study should be a useful tool for macrophage research.

Comparative evaluation of smear layer removal by chitosan and ethylenediaminetetraacetic acid when used as irrigant and its effect on root dentine: An in vitro atomic force microscopic and energy-dispersive X-ray analysis.

Aim:This study was undertaken to evaluate the efficacy of smear layer removal and nanostructural and chemical changes caused by chitosan and ethylenediaminetetraacetic acid (EDTA) on tooth surface using atomic force microscopic analysis and energy-dispersive X-ray (EDX) analysis.Methodology:Forty single-rooted premolars were decoronated to a standard length of 15 mm and enlarged to Protaper F3 with irrigation of 1 mL 1% NaOCl and deionized water. Specimens were then divided into 4 groups with 10 samples each and subjected to final rinse with 17% EDTA solution, 0.2% and 0.5% chitosan solution for 1 min. Samples were sectioned into 2 halves. One half of sample from each group were subjected to EDX analysis to check the calcium/phosphate (Ca/P) ratio. The second half of sample from each group subjected to atomic force microscopy (AFM) analysis to study the smear layer removal and nanostructural changes. Statistical analysis was done using ANOVA and Chi-square test.Results:The AFM images showed no difference in the elimination of smear layer. The quantitative analysis using AFM showed EDTA group had significantly higher surface alteration than chitosan. EDX analysis showed that the Ca/P ratio of root dentine in EDTA group is significantly lower than chitosan group.Conclusion:Chitosan is an effective chelating agent with less alteration in radicular dentine.

An in vitro study to evaluate the demineralizing effect of maleic acid and cell attachment on cemental surface

Introduction: Root biomodifying agents are adjuncts to periodontal regeneration. Aim: This study evaluates the demineralization effect of a chelating agent, maleic acid (MA) when used as a root biomodifier on the cemental surface. Materials and Methods: Thirty single-rooted anterior teeth were selected for this study. The teeth were decoronated and split longitudinally to form sixty radicular halves. They were randomly assigned to four groups of 15 fragments each according to the demineralizing agents used and treatment time of 1 and 3 min. Group 1 treated with 50% citric acid (CA) solution at pH 1, Group 2 treated with 17% ethylenediaminetetraacetic acid (EDTA) solution at pH 7. Group 3 treated with 7% MA at pH 1.3 and Group 4 treated with saline. The specimens were then prepared to view under scanning electron microscopy (SEM). To study the adherence of cells on the root surface, human periodontal ligament cells were cultured and were placed in plates containing root halves, and cell adherence was viewed using SEM. The data were statistically analyzed by Pearson's Chi-square test (χ2) to evaluate the effect of the each test agent. A significance level was set at P Results: All the three test agents were successful in eliminating smear layer at both time intervals. Intergroup comparison at both time intervals demonstrated, MA to have a better smear layer removal ability than CA and EDTA. Conclusion: This particular study provided preliminary evidence on the demineralizing effect of 7% MA when used as a root conditioning agent.

The composition of organic phosphorus in soils of the Snowy Mountains region of south-eastern Australia

Few studies have considered the influence of climate on organic phosphorus (P) speciation in soils. We used sodium hydroxide–ethylenediaminetetra-acetic acid (NaOH–EDTA) soil extractions and solution 31P nuclear magnetic resonance spectroscopy to investigate the soil P composition of five alpine and sub-alpine soils. The aim was to compare the P speciation of this set of soils with those of soils typically reported in the literature from other cold and wet locations, as well as those of other Australian soils from warmer and drier environments. For all alpine and sub-alpine soils, the majority of P detected was in an organic form (54–66% of total NaOH–EDTA extractable P). Phosphomonoesters comprised the largest pool of extractable organic P (83–100%) with prominent peaks assigned to myo- and scyllo-inositol hexakisphosphate (IP6), although trace amounts of the neo- and d-chiro-IP6 stereoisomers were also present. Phosphonates were identified in the soils from the coldest and wettest locations; α- and β-glycerophosphate and mononucleotides were minor components of organic P in all soils. The composition of organic P in these soils contrasts with that reported previously for Australian soils from warm, dry environments where inositol phosphate (IP6) peaks were less dominant or absent and humic-P and α- and β-glycerophosphate were proportionally larger components of organic P. Instead, the soil organic P composition exhibited similarities to soils from other cold, wet environments. This provides preliminary evidence that climate is a key driver in the variation of organic P speciation in soils.

Determination of Lead Desorption from <i>G. fisheri</i> Seaweed Using Edible Eluents by Voltammetry at the Hanging Mercury Drop Electrode

A simple, rapid, selective and sensitivity approach for the determination of Pb(II) in G. fisheri seaweed is described. The method is based on differential pulse anodic stripping voltammetry (DPASV) at hanging mercury drop electrode (HMDE) vs. Ag/AgCl in 0.2 M ammonium acetate (NH4OAc) pH 7.5. The operating analytical conditions; deposition potential (Edep) of -0.4 V, peak potential of -0.78 V, and mercury dropped size of 3 were performed. To see the sensitivity of Pb(II) measurement, the influences of deposition time and stirring speed were investigated. From the findings, the optimal parameters; deposition time of 90 s, and stirring speed of 2000 rpm were obtained. In these conditions, the limit of detection (3σ) of 0.60 µgL-1 and the linear range extended to 12.50 µgL-1 (r2=0.9999) were obtained. The relative standard deviation (RSD) of triplicate measurements using 1.8 µgL-1 of Pb(II) was 1.22%. The method was then applied to measure Pb(II) in real samples. In this study, the desorption efficiency of edible eluents by batch method was determined. The method is based on Pb(II) desorption using different types of edible eluents; acetic acid (HOAc), citric acid (CTA), sodium chloride (NaCl), sodium bicarbonate (NaHCO3), ethylenediaminetetraacetic acid (EDTA), and chitosan (CTS). Batch desorption of Pb(II) from seaweed soaked in individual eluent was performed by shaking at 100 rpm for 2 h at ambient temperature. Results show that the most effective eluent in desorbing the contaminated Pb(II) from G. fisheri with up to 82% of desorption efficiency for bound Pb(II) was EDTA solution.

Effect of irrigation on surface roughness and fatigue resistance of controlled memory wire nickel-titanium instruments.

To investigate the effect of irrigation on the surface roughness and fatigue resistance of HyFlex and M3 controlled memory (CM) wire nickel-titanium instruments. Two new files of each brand were analysed by atomic force microscopy (AFM). Then, the instruments were dynamically immersed in either 5.25% sodium hypochlorite (NaOCl) or 17% ethylene diamine tetraacetic acid (EDTA) solution for 10min, followed by AFM analysis. The roughness average (Ra) and root mean square (RMS) values were analysed statistically using an independent sample t-test. Then, 36 files of each brand were randomly assigned to three groups (n=12). Group 1 (the control group) was composed of new instruments. Groups 2 and 3 were dynamically immersed in 5.25% NaOCl and 17% EDTA solutions for 10min, respectively. The number of rotations to failure for various groups was analysed using the one-way analysis of variance software. For M3 files, the Ra and RMS values significantly increased (P<0.05) after the immersion. For the HyFlex file, the Ra and RMS values significantly increased (P<0.05) only in EDTA, but not (P>0.05) NaOCl. The resistance to cyclic fatigue of both HyFlex and M3 files did not significantly decrease (P>0.05) by immersing in 5.25% NaOCl and 17% EDTA solutions. Except the HyFlex files immersed in NaOCl, the surface roughness of other files exposed to irrigants increased. However, a change in the surface tomography of CM wire instruments caused by contact with irrigants for 10min did not trigger a decrease in cyclic fatigue resistance.

An Invitro Evaluation of the Accuracy of Two Electronic Apex Locators to Determine Working Length in the Presence of Various Irrigants.

Cleaning and shaping of root canal system requires various irrigating solutions. The question is whether the presence of irrigation solution alters working length determination using apex locators. The purpose of this study was to compare the accuracy of Root ZX and i-Root apex locator for determining working length in the presence of different irrigating solution. Eighty extracted single rooted human teeth were used. The teeth were sectioned at Cemento Enamel Junction (CEJ) and actual canal length determined. Then, working length measurements were obtained using Root ZX and i-Root apex locator in the presence of irrigating solutions namely 0.9% saline, 3% of sodium hypochlorite (NaOCl), 2% chlorhexidine digluconate(CHX) and 17% Ethylene Diamine Tetra Acetic acid (EDTA). The measurements obtained with Root ZX and i-Root apex locator were compared with actual canal length and subjected to statistical analysis using Post Hoc Test Tukey's Method. This study revealed that both the tested Electronic apex locators (EAL) were able to measure the canal length in the presence of tested irrigating solutions. The presence of irrigating solutions of saline, NaOCl, chlorhexidine and EDTA in the root canal marginally influenced the accuracy of the Root ZX or i-Root (P < 0.36), but with no clinical significance. Root ZX and i-Root can be used safely to determine working length in the presence of various irrigants. The content of the root canal did not influence the accuracy while measuring working length using Electronic apex locators (EAL).

The use of ozone in a continuous cyclical swing mode regeneration of Fe-EDTA for a clean biogas process from a swine farm waste

Hydrogen sulfide in biogas causes environmental problem such as acid rain, therefore several methods are employed in the process to eliminate hydrogen sulfide in biogas. A process for hydrogen sulfide removal in clean biogas production has been examined at a swine farm. Ferric chloride – Ethylenediaminetetraacetic acid solution was used to oxidize hydrogen sulfide to elemental sulfur and ozone was used in the regeneration of ferrous ion to ferric ion. The experiments were conducted using 2 bubbling columns with the same diameter and height, which were 52 mm and 610 mm, respectively. The process was run in a continuous cyclical swing mode. The flow rate of biogas was fixed at of 0.5 L/min and hydrogen sulfide concentrations in the biogas were in the range of 4000–5000 ppm. The initial pH of the Ferric chloride – Ethylenediaminetetraacetic acid solution was varied from 6.5 to 9. Experimental results in the hydrogen sulfide removal part, indicated that Ferric chloride – Ethylenediaminetetraacetic acid solution achieved a maximum 99.5% hydrogen sulfide removal efficiency at pH 8, with a criteria that hydrogen sulfide in the biogas must be less than 100 ppm. In the regeneration part, the regeneration of ferric ion from ferrous ion using ozone in air stream was successfully obtained. The regeneration of ferric ion from ferrous ion was completed within 10 min and the pH turned to pH 8. The regenerated Ferric chloride – Ethylenediaminetetraacetic acid solution was reused several times and still showed high ability for hydrogen sulfide removal. The key parameter, that had to be monitored, was pH, which was relevant to ferrous precipitation. The goal of the process was achieved since hydrogen sulfide was reduced to not exceed 100 ppm, without altered methane concentration in the biogas. Ozone was successfully applied in a continuous cyclical swing mode for the first time in the hydrogen sulfide removal in the biogas production.

Release of Pb in soils washed with various extractants

The suitability of an agent for washing heavy metal contaminated soils depends not only on the efficiency of heavy metal removal but also on the persistence of the washing effect, especially for agricultural soil. Redistribution of residual heavy metals in washed soil from inert pools to labile pools can reverse the initial washing effect. In order to study the redistribution of residual Pb in Pb-contaminated soils washed with citric acid (CA), ethylenediaminetetraacetic acid (EDTA), FeCl3, and HCl solutions, washed soils were incubated under both flood and 70% field water capacity (FWC) conditions for 180days. The Pb availability in CA-, EDTA-, FeCl3-, and HCl-washed soils varied with incubation time. Diethylenetriaminepentaacetic acid (DTPA)-Pb generally increased with incubation time, except in CA-washed soil under flood incubation condition. Pb associated with the exchangeable/acid extractable fraction (F1) increased, whereas Pb in the easily reducible fraction (F2) decreased over time, for all washed soils under flood incubation condition; the changes in the amount of Pb associated with each fraction were much smaller under 70% FWC incubation. The transformation of Pb from F2 to F1 during flood incubation was largely caused by the mobilization of Fe and Mn oxides. The mobility factors (MF) of Pb for samples under flood incubation increased with increasing incubation time while those for samples under 70% FWC incubation changed only slightly, which implies increasing environmental risks of Pb from washed soils that are flooded. Therefore, the redistribution and potential environmental risks posed by heavy metals in washed soils, especially in paddy rice soils, should be taken into account when the suitability of a washing agent is evaluated. In the present study, CA was found to be unsuitable for washing Pb-contaminated agricultural soil, whereas the FeCl3 solution was the most stable washing agent tested, as it had high removal efficiency and low release of Pb after washing.

Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography

The aim of this study was to analyze the effectiveness of decalcification using ethylenediaminetetraacetic acid (EDTA) as an optical clearing method to enhance the depth visibility of internal soft tissues of cochlea. Ex vivo mouse and guinea pig cochlea samples were soaked in EDTA solutions for decalcification, and swept source optical coherence tomography (OCT) was used as imaging modality to monitor the decalcified samples consecutively. The monitored noninvasive cross-sectional images showed that the mouse and guinea pig cochlea samples had to be decalcified for subsequent 7 and 14 days, respectively, to obtain the optimal optical clearing results. Using this method, difficulties in imaging of internal cochlea microstructures of mice could be evaded. The obtained results verified that the depth visibility of the decalcified ex vivo samples was enhanced.

Preparation of DNA-immobilized magnetic particles and their utilization as an accumulative material of metal ions

Abstract

Study of the Influence of the Electrolysis Parameters on Mn-Zn, Mn-Cu and Mn-Cu-Zn Alloys Coatings from Electrolytes Containing Complexing Ligands

The process of obtaining of high quality Mn-Zn, Mn-Cu and Mn-Cu-Zn alloy coatings from complexing ligands-citrate, EDTA (ethylene diaminetetra acetic acid) and nitrilotriacetic acid solutions was studied. Factors affecting stability of solutions containing ligand or ligands and influence of electrolysis parameters: electrolyte composition, pH, cathodic current density on chemical composition of the obtained coatings, on their current efficiency, morphology and structure were investigated.

Removal of MTBE from a clay soil using electrokinetic technique

ABSTRACTRemediation of a soil contaminated with methyl tertiary butyl ether (MTBE) was studied by using the electrokinetic technique. A series of experimental tests were carried out on contaminated soil in an electro-osmotic apparatus at different applied gradients of voltage and time. The tests were conducted with distilled water and ethylenediaminetetra acetic acid (EDTA) solution as electrolyte. During each test the values of pH at anode and cathode reservoirs and also the discharge from cathode were measured. At the end of each test a number of soil samples were extracted from the middle of the soil at different distances from the anode and the removal of contaminant was measured by a gas chromatography apparatus. The results indicate that with EDTA as electrolyte the highest efficiency for removal of MTBE is achieved with 2.0 V/cm gradient and in the duration of 14 days. In addition, EDTA causes the values of pH to increase and decrease in the cathode and anode reservoirs, respectively. It also decreases the effluent and electro-osmotic permeability in comparison with distilled water. Experimental data were analysed by ANOVA and t-test methods. These statistical analyses showed significant difference (at 5% level) between the reference and other tests.

A SEM evaluation of smear layer removal using two rotary instrument systems with EDTA and vinegar as a root canal irrigant

Introduction: As the smear layer (SL) produced during instrumentation adheres to the dentinal surface and prevents penetration of irrigants and medicaments, it should be removed. The aim of this study was to evaluate the ability of 17% ethylenediaminetetraacetic acid (EDTA) and white vinegar (5% pure acetic acid) to remove debris and SL produced during root canal preparation with two nickel-titanium (NiTi) file systems, Hyflex (Coltene Whaledent, Allstetten, Switzerland) and ProTaper (Dentsply, Tulsa, OK, USA). Materials and Methods: Twenty freshly extracted human, single-rooted teeth were sectioned at the cementoenamel junction and were randomly divided into four groups of five samples each. In each group, 0.5 mL of 5% NaOCl was used as the first irrigant, which was followed by either 17% EDTA or vinegar. Two groups were prepared with Hyflex rotary files and the other two with ProTaper files. Scanning electron microscopy (SEM) study was done to evaluate the SL, and the collected data were submitted for statistical analysis. Statistical analysis was performed based on Student's t-test with P value >0.05. Results: There was no statistically significant difference between the types of instruments used (Hyflex files and ProTaper files), and 17% EDTA solution and vinegar (5% acetic acid). Conclusion: Vinegar can be relied on as an endodontic irrigant for SL removal was comparable to 17% EDTA. In addition, both types of NiTi instrument produced similar dentin surface in terms of SL when used along with EDTA and vinegar.

Effect of phytic acid, ethylenediaminetetraacetic acid, and chitosan solutions on microhardness of the human radicular dentin.

Aim:The purpose of this study was to evaluate the effect of phytic acid, ethylenediaminetetraacetic acid (EDTA), and chitosan solutions on the microhardness of human radicular dentin.Materials and Methods:Thirty dentin specimens were randomly divided into three groups of 10 specimens each according to the irrigant used: G1 — 1% phytic acid, G2 — 17% EDTA, and G3 — 0.2% chitosan. A standardized volume of each chelating solution was used for 3 min. Dentin microhardness was measured before and after application at the cervical, middle, and apical levels with a Vickers indenter under a 200-g load and a 10-s dwell time. The results were analyzed using one-way analysis of variance (ANOVA) and Student's t test.Results:Microhardness of the radicular dentin varied at the cervical, middle, and apical levels. EDTA had the greatest overall effect, causing a sharp percentage reduction in dentin microhardness with a significant difference from phytic acid and chitosan (P = 0.002). However, phytic acid and chitosan differed insignificantly from each other (P = 0.887).Conclusion:All tested chelating solutions reduced microhardness of the radicular dentin layer at all the levels. However, reduction was least at the apical level. EDTA caused more reduction in dentin microhardness than chitosan while phytic acid reduced the least.

Scanning Electron Microscopic Evaluation of Root Canal Walls after shaping with Different Single-use Rotary Systems

ABSTRACT The aim of the present study was to investigate the cleaning efficacy of four new single-use rotary nickel–titanium (NiTi) systems. A total of 48 single-rooted freshly extracted teeth were divided into four groups. Root canals were shaped with the single-use NiTi systems (ProTaper Next, F360, OneShape and F6 SkyTaper) using 5.25% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA) solutions. Specimens were fractured longitudinally and prepared for scanning electron microscopic (SEM) analysis at standard magnification of 2,500?. The presence/absence of debris smear layer and smear layer at coronal, middle, and apical third of each canal were evaluated using a five-step scale for scores. Numeric data were analyzed using Kruskal–Wallis and Mann–Whitney U statistical tests and significance was predetermined at p&lt; 0.05. Analysis of variance test showed no significant differences among the NiTi systems (p &gt; 0.05). The same results were assessed considering the smear layer scores. Analysis of variance confirmed that the apical third of the canal maintained a higher quantity of debris and smear layer after preparation of all the samples. All the single-use rotary NiTi systems left dentinal walls generally free of debris, even if a small amount of smear layer is visible, especially in the apical third of the root canal. All tested single-use systems seem to be effective in removing smear layer from root canals, if irrigating protocols are respected. How to cite this article Dagna A, Gastaldo G, Beltrami R, Bianchi S, Poggio C. Scanning Electron Microscopic Evaluation of Root Canal Walls after shaping with Different Single-use Rotary Systems. Int J Experiment Dent Sci 2016;5(2):93-98.

Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro.

There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.

Impact of Various Irrigating Agents on Root Fracture: An in vitro Study.

Irrigating solutions are used for cleaning and removing dentinal debris, and the other remains from pulpal space during biomechanical preparation. Therefore, we evaluated the impact of various irrigating agents on root fracture at 5-minute time exposure. We sectioned 60 permanent maxillary premolars with fully formed root structures transversely maintaining the root length of approximately 14 mm. Five study groups were made comprising ethylenediaminetetraacetic acid (EDTA), cetrimide, citric acid, and so on as various irrigating agents. A universal force test machine was used to calculate the force which was enough to fracture each root. Analysis of variance (ANOVA) test was used to access the level of significance. About 10% citric acid solution as an irrigating agent showed minimal fracture opposing results, whereas 10% EDTA solution showed the maximum fracture resistance of root portion. Selection of suitable EDTA concentration that has minimal adverse effect on the mechanical properties of the tooth is very important for the successful management of tooth fracture. About 10% EDTA provided the highest fracture resistance, necessitating the use of irrigating solution in root canal therapy (RCT). Further research with higher and different study groups is required to search for more efficient irrigating solution to improve the outcome of RCT.

The Coffee Protective Effect on Catalase System in the Preneoplastic Induced Rat Liver

This study aimed to evaluate the effect of organic/conventional coffee in liver tissues in the cancer process, taking into account the level and activities of catalase. The experiments were carried out with 8 groups of rats during 12 weeks. They received two injections of ethylenediaminetetraacetic acid solution 1.5% (v/v) prepared in 0.9% NaCl or 1,2-dimethylhydrazine (DMH) subcutaneous dose of 40 mg·kg−1·bw−1for 2 weeks. The organic/conventional coffee infusions were at 5, 10, and 20% and were incorporated to feed (100 mL of infusion·kg−1of diet). The catalase activity showed a decrease for livers which received DMH and DMH plus organic coffee at 5% and 10%. However, an increase was observed for those receiving organic 20% and conventional 10% coffee, slowing down and favoring the reversibility of the carcinogenic process. By SDS-PAGE, we observed an intensity decrease of 59 kDa bands, as the percentage of coffee was increased. The iron concentration (by ET-AAS) confirmed the electrophoretic results, suggesting that the DMH influenced the catalase expression conditions, reducing the activity by the loss of iron ions. Thus, the coffee may restore the catalase system in the liver, exerting its chemopreventive effects.