Ethyl Acetate Fraction Of Methanol Extract Research Articles

Ameliorative role of ethyl-acetate fraction of methanolic leaf extract of Camellia sinensis (green tea) on streptozotocin-induced diabetes linked testicular hypofunction in albino rat: A dose-dependent biochemical and genomic transection study.

Background The present investigation focuses the diabetes-induced testicular hypofunction and its possible correction by the effective dose of ethyl-acetate fraction of methanolic extract of Camellia sinensis leaves through dose-dependent study in streptozotocin-induced diabetic rat. Methods The androgenic, spermiological, oxidative stress and apoptosis sensors along with testicular genomic sensors were evaluated in a dose-dependent fashion (50 mg or 100 mg or 200 mg/kg body weight). Activities of hepatic transaminases for toxicity assessment were also measured. Results Increased level of fasting blood glucose, testicular cholesterol, seminal vesicular fructose along with a low count, motility and viability of epididymal sperm, low activities of testicular Δ5, 3β-hydroxy steroid dehydrogenase (HSD), 17β-HSD, testicular antioxidant enzymes (catalase and superoxide dismutase) and low plasma level of testosterone were noted in diabetic rat in respect to the control. After oral administration of said fraction to diabetic rat, levels of above sensors were resettled toward the control. A significant decrease in the number of different generations of germ cells at the stage VII of spermatogenesis in diabetic rat was noted which were recovered significantly toward the control in the fraction-treated diabetic group. It was supported by the correction in gene expression of testicular Δ5, 3β- HSD, 17β- HSD, Bcl-2 and Bax in the fraction-treated diabetic group. Conclusions The threshold dose of ethyl-acetate fraction of methanolic extract of C. sinensis leaves is 100 mg/kg body weight for the recovery of testicular hypofunction in a diabetic rat model.

Evaluation of antidiabetic and antioxidative efficacy of ethyl acetate fraction of methanolic extract of Camellia sinensis (green tea) leaves in streptozotocin induced diabetic albino rat

<p><strong>Objective:</strong> To investigate the antidiabetic and antioxidative potentiality of ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves in streptozotocin induced diabetic rat. <strong>Methods:</strong> Streptozotocin induced diabetic state was confirmed by increased level of fasting blood glucose, decreased level of serum insulin along with inhibition in carbohydrate metabolomics. Oxidative stress was assessed by measuring antioxidative enzyme activities of hepatic and skeleto-muscular tissue. Hepatic <em>Hexokinase-I</em>, pro-apoptotic <em>Bax</em> and anti-apoptotic <em>Bcl-2</em> gene expression patterns were noted by qRT-PCR technique. <strong>Results:</strong> After treatment with ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves at a dose of 100 mg/kg body weight/day to diabetic rats for 28 days, a significant (p < 0.05) recovery was noted in fasting blood glucose level, serum insulin level along with activities of carbohydrate metabolic enzymes in hepatic tissue in respect to the vehicle treated diabetic group. This fraction also resulted a significant (p < 0.05) recovery in the activities of antioxidative enzymes in hepatic and skeleto - mascular tissue. In streptozotocin induced diabetic rat the low level of expression of <em>Hexokinase-I</em>, anti-apoptotic <em>Bcl-2</em> and high level of expression of pro-apoptotic <em>Bax</em> gene were observed in hepatic tissue in respect to vehicle treated control. There were recovered significantly after the treatment with the said fraction. <strong>Conclusion: </strong>From the results, it may be concluded that ethyl-acetate fraction of methanolic extract of leaves of <em>C. sinensis</em> has a promising anti-diabetic and antioxidative activities for the management of streptozotocin induced diabetic state.</p>

Comparative HPTLC Analysis of Leaves of Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. with Standard Quercetin

Flavonols aglycones are well established in plant extracts with omnipresence of common flavonols like kaempferol, quercetin and myricetin. Quercetin has been reported for various pharmacological activities with major ones in the field of inflammation, cancer, arthritis, diabetes and its complications. Leaves of plants selected for this study viz. Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. reported to have antioxidant, anticancer, hypoglycemic activities. It is hypothetised thisactivities are due to flavonoids present in these plants. Hence the ethylacetate fraction of methanolic extract of these leaves was obtained as it provides flavanoid rich fraction and the samples were prepared and HPTLC analysis was performed against standard quercetin and their quantitative analysis was performed, the solvent system toluene: ethyl acetate: formic acid (9: 1: 0.5) was selected. It was observed that these plants shows presence of quercetin where A. cepa has highest concentration with F. carica and Z. mauritiana having quercetin in declining order. The calibration curve was linear in the range of 0.1 to 0.7 μg for quercetin. Further a correlation coefficient of 0.999 indicates good linearity between concentration and area. Thus, it was proved that the plants used in the study has presence of quercetin which is possibly the reason having the pharmacological uses.

In vitro anti-oxidant activity of Ruellia tuberosa root extracts

Background: The present study was aimed to evaluate in vitro anti-oxidant activity of the Ruellia tuberosa (Acanthaceae) roots. Materials and Methods: Anti-oxidant activity was evaluated by using DPPH free radical scavenging activity and reducing power by FeCl3. The methanolic extract (ME), water extract (WE), ethyl acetate extract (EAE) and ethyl acetate fraction of methanol extract (EAFME) of root were tested. The Ascorbic acid was used as positive control. Total phenolic and total flavonoid content were also determined by Folin-Ciocalteu reagent and complementary colorimetric methods (aluminum chloride method and 2, 4-dinitrophenylhydrazine method respectively. Results: The EAFME of root showed the highest concentration of phenolic, flavonoid content, free radical scavenging activity and reducing power. The various extract showed a significant anti-oxidant activity when (P < 0.05) compared with standard. Conclusion: It is concluded that the R. tuberosa root possess anti-oxidant activity. Further studies are suggested to isolate the active principle responsible for the activity.