Abstract
<p><strong>Objective:</strong> To investigate the antidiabetic and antioxidative potentiality of ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves in streptozotocin induced diabetic rat. <strong>Methods:</strong> Streptozotocin induced diabetic state was confirmed by increased level of fasting blood glucose, decreased level of serum insulin along with inhibition in carbohydrate metabolomics. Oxidative stress was assessed by measuring antioxidative enzyme activities of hepatic and skeleto-muscular tissue. Hepatic <em>Hexokinase-I</em>, pro-apoptotic <em>Bax</em> and anti-apoptotic <em>Bcl-2</em> gene expression patterns were noted by qRT-PCR technique. <strong>Results:</strong> After treatment with ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves at a dose of 100 mg/kg body weight/day to diabetic rats for 28 days, a significant (p &lt; 0.05) recovery was noted in fasting blood glucose level, serum insulin level along with activities of carbohydrate metabolic enzymes in hepatic tissue in respect to the vehicle treated diabetic group. This fraction also resulted a significant (p &lt; 0.05) recovery in the activities of antioxidative enzymes in hepatic and skeleto - mascular tissue. In streptozotocin induced diabetic rat the low level of expression of <em>Hexokinase-I</em>, anti-apoptotic <em>Bcl-2</em> and high level of expression of pro-apoptotic <em>Bax</em> gene were observed in hepatic tissue in respect to vehicle treated control. There were recovered significantly after the treatment with the said fraction. <strong>Conclusion: </strong>From the results, it may be concluded that ethyl-acetate fraction of methanolic extract of leaves of <em>C. sinensis</em> has a promising anti-diabetic and antioxidative activities for the management of streptozotocin induced diabetic state.</p>
Highlights
Camellia sinensis (C. sinensis) L. belongs to the Theacea family has immense medicinal properties in the treatment and prevention of many diseases [1]
For the measurement of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities, kits were used supplied by Creast Bio-systems, Gitanjali, Dr Antonio Do RegoBagh, Alto Santacruz, Bambolim Complex (Goa, India) [17]
Fasting blood glucose was significantly (p
Summary
Camellia sinensis (C. sinensis) L. belongs to the Theacea family has immense medicinal properties in the treatment and prevention of many diseases [1]. Diabetes mellitus (DM), a chronic metabolic disorder, called syndrome is characterised by hyperglycaemia. This is mainly caused by insulin deficiency which is often combined with insulin resistance [2]. Recent decades have shown a resurgent interest in traditional plant medicine for the management of hyperglycaemia and oxidative stress [5]. The World Health Organization (WHO) recommends that this practice should be encouraged especially in countries where access to DOI:10.5138/09750185.2025 conventional treatment of diabetes mellitus is not adequate [6]. On that perspective the present study was designed to investigate the therapeutic potentiality of the ethyl acetate fraction of methanolic extract of C.sinensis (Green tea) leaves against hyperglycaemia and oxidative stress condition in experimental model diabetic rats
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