Ethanol Extract Of Plant Research Articles

Spectrophotometric Detection of Beta-Lactamase Inhibition by Plant Extracts

Background: Antibiotic-resistant bacteria have been spreading with increasing frequency over the past several decades. The decreasing effectiveness of therapies in treating bacterial resistance, due to the production of beta-lactamase (EC.3.5.2.6), has led to combining beta-lactamase inhibitors with commonly used beta-lactam antibiotics. Phytochemical compounds produced by plants have varied biological properties such as antioxidant, antimicrobial, antidiabetic, anti-inflammatory, anticancer, antihypertensive and others. Polyphenolics, flavonoids, coumarins, alkaloids, tannins, steroids and terpenoids are the main phytochemicals found in plants extracts. This study aims to assess the inhibitory activity of Annona senegalensis Pers., Terminalia superba Engl. & Diels, Ipomoea batatas Lam., and Psidium guajava L. against a Bacillus cereus beta-lactamase and four beta-lactamases extracted from clinical isolates of Escherichia coli. Methods: Beta-lactamase inhibition was measured using a spectrophotometric assay with benzylpenicillin as the substrate, by a modified acidimetric method. Clavulanic acid was used as the standard beta-lactamase inhibitor. Subsequently, the inhibition of the hydrolysis of other commonly used beta-lactam antibiotics was assessed using a UV spectrophotometric method. Results: Among the tested extracts, Ipomoea batatas showed no effect on the beta-lactamases, whereas Terminalia superba and Annona senegalensis exhibited inhibitory activity against all the beta-lactamases. Psidium guajava inhibited two of the five tested enzymes. The highest percentage of inhibition was observed with Terminalia superba on the beta-lactamase of Bacillus cereus, reaching 75.73%. Regarding the combination of beta-lactam antibiotics with plant extracts, the combination of Terminalia superba and ceftriaxone showed the highest inhibition, with a percentage of 64.90%. The combination of Annona senegalensis and ceftazidime was the least effective, with an inhibition rate of only 12.95%. Conclusion: Two of the tested ethanolic plant extracts showed interesting beta-lactamase inhibitory effects and could contribute to the development of more effective therapies against beta-lactamase-producing bacteria by combining natural inhibitors from plants with existing beta-lactam antibiotics.

Down-regulation of interlinked inflammatory signalling cascades by ethanolic extract of Suaeda fruticosa Forssk. ex J.F. Gmel. attenuated in vivo inflammatory and nociceptive responses.

Juice and decoction of leaves of Suaeda fruticosa, a halophytic medicinal plant of Cholistan desert, is traditionally used to treat rheumatism. The current study was carried out to probe into in vivo anti-nociceptive, anti-inflammatory, and anti-arthritic potential of ethanolic extract of the whole plant of S. fruticosa (Et-SF)and its bioactive molecules. GC-MS screening of Et-SF revealed presence ofvarious bioactive compounds including phytol, thymol, n-hexadecanoic acid,farnesol, and 1-heptacosanol. DPPH in vitro radical scavengingassay demonstrated moderate antioxidant potential of Et-SF. Safety evaluation of Et-SF confirmed no lethal effects in female albino rats up to the singleoral dose of 5000mg/kg. In all invivo models, Et-SF was administered in three doses (125, 250, and 500mg/kg) and a single dose of flurbiprofen (FP) (10mg/kg). Et-SF significantly (p < 0.05) attenuated acute inflammation in carrageenan, histamine, and serotonin-induced rat paw oedema models in a time-dependent manner. Et-SF alleviated oedema, restored haematological parameters, and reduced severe pannus formation, inflammatory cell infiltration, and fibrous tissue proliferation inthe paws of CFA-induced arthritic rats.Moreover, treatment with thymol, farnesol and n-hexadecanoic acid alone and in combination also attenuated the arthritic progression in the arthritic rats indicating involvementof these compounds towards anti-arthritic potential of Et-SF. Et-SF and FP significantly (p < 0.05) down-regulated IL-1β, TNF-α, IL-6, NF-κB, and COX-2 mRNA expression, and up-regulated IL-4 and IL-10 mRNA expression in arthritic rats. Hot plate and acetic acid-induced writhing models results indicated the analgesic attributes of Et-SF in mice models. This study suggests that S. fruticosa ethanol extract may regulate the expression ofinflammatory markers involved in nociceptive, inflammatory, and arthritic disorders. Its phytochemicals could target multiple phases of these conditions at cellular and subcellular levels. Further research is needed to confirm this hypothesis.

ANTIOXIDANT ACTIVITY OF 96% ETANOL EXTRACT OF KROKOT PLANT (Portulaca oleraceae L.) IN FACE SPRAY PREPARATION BY DPPH METHOD

Krokot plants (Portulaca oleracea L.) are weeds that are commonly found in rice fields, plantations, and home yards. Krokot plants have many benefits, including their use as antirheumatic, antibacterial, and antioxidant drugs. The secondary metabolites contained in krokot plants, namely flavonoids, act as the main bioactive compounds. This study aimed to determine the physical properties of face spray preparations and the antioxidant activity of extracts and face spray preparations of 96% ethanol extract of krokot plant (Portulaca oleracea L.) to prevent free radicals. The research method used was Descriptive and inferential analyses were conducted in this study. Krokot plant extract was prepared by maceration using 96% ethanol solvent at a ratio of 1:10 and then used as the active ingredient of formulation (F) at various concentrations of face spray formulations. F1 as negative control, F2 at 0.7%, F3 at 1.4%, and F4 at 2.8%. The results of the physical evaluation of face spray preparations for all formulas met the requirements (organoleptic, pH, homogeneity, dry time test, viscosity, and spray spreadability test). The antioxidant activities of face spray preparations in F1, F2, F3, and F4 had IC50 values of 110.914, 106.581, 85.261, and 64.936 ppm, respectively. The conclusion of this study is that the extract and face spray preparation formula of 96% ethanol extract of krokot plant (Portulaca oleracea L.) have antioxidant activity. Formula 4 showed the best physical properties of face spray preparation. Keywords: Formulation, krokot plant extract, face spray, antioxidant.

In Vitro Assessment of Antimicrobial Activity and Synergistic Effects of Ethanolic Extracts from Six Medicinal Plants

This in vitro experimental study examines and evaluates the antimicrobial and synergistic effects of the ethanolic extract of six plants: Biancaea sappan (L.) Tod, Bauhinia malabarica Roxb, Carthamus tinctorius L., Derris scandens (Roxb.) Benth, Hibiscus sabdariffa L., and Piper nigrum L. against common microbial species representing gram-positive, gram-negative bacteria, and fungi, consisting of Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans. The plants were extracted using 90% ethanol. According to the standard method of agar diffusion assay, the micro-dilution method for minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. This study found that among the six plants, only B. sappan and B. malabarica exhibited moderate inhibitory effects against S. aureus and S. epidermidis. B. sappan had MIC values of 250 µg/ mL and 125 µg/ mL, respectively, and B. malabarica showed MIC values of 62.50 µg/ mL and 31.25 µg/ mL, respectively. The synergistic effects of a combination of B. sappan and B. malabarica extracts at a ratio of 25:75 were analyzed, and it was found that the combination inhibited S. aureus and S. epidermidis with MIC values of 250 µg/ mL and 125 µg/ mL, respectively. The fractional inhibitory concentration index (FICI) and the fractional bactericidal concentration index (FBCI) indicated antagonistic or synergistic effects of the combination, with FICI and FBCI values of 2.5–5.0 for both B. sappan and B. malabarica extracts in the 25:75 mixture. In conclusion, single plant ethanolic extracts of B. sappan and B. malabarica possess potent antimicrobial activity to varying degrees. However, the antimicrobial potency of the 25:75 ratio mixture of these extracts was shown to decrease against the same organisms, with in vitro antimicrobial activity and antagonistic effects observed only against the tested gram-positive bacteria.

Chromatographic profiling of bioactive polyphenolic compounds and antioxidant evaluation of ethnobotanical leafy vegetables (Amaranthus spinosus and Glinus oppositifolius) from the hilly regions of Bangladesh

Amaranthus spinosus and Glinus oppositifolius are leafy vegetables commonly consumed by indigenous populations in the Bandarban hill tracts of Bangladesh due to their distinct sensory characteristics and therapeutic properties. This study aimed to analyze the bioactive polyphenolic compounds in ethanolic extracts of both plants using HPLC-DAD and evaluate their antioxidant potential. Results revealed that G. oppositifolius had higher total flavonoid content, total phenolic content, total antioxidant activity and IC50 values compared to A. spinosus. However, A. spinosus exhibited a higher total tannin content. The chromatographic analysis identified various bioactive compounds, including gallic acid, epicatechin, quercetin, catechin, rutin hydrate, kaempferol, caffeic acid, and ferulic acid in A. spinosus. Similarly, G. oppositifolius contained gallic acid, epicatechin, quercetin, catechin, rutin hydrate, caffeic acid, trans-ferulic acid, rosmarinic acid, trans cinnamic acid, and vanillin. These polyphenol-rich extracts possess significant antioxidant and anti-inflammatory properties, making them promising candidates for nutraceuticals, functional foods, and industrial applications.

Standardization and HPTLC Fingerprint Studies on the Ethanol Extracts of &lt;i&gt;Vitex negundo&lt;/i&gt; Leaves and &lt;i&gt;Opuntia dillenii&lt;/i&gt; Cladode

Background: In traditional Indian system of medicine Vitex negundo Linn is referred as Sarvaroganivarani - the remedy for all diseases. The cladodes of Opuntia dillenii possess anti-inflammatory, potent hypotensive and antihyperglycemic effects. Aim: This study aimed to perform comparative standardization and HPTLC fingerprinting on ethanolic leaf extracts of Vitex negundo and cladodes of Opuntia dillenii. Methods: Extracts were prepared using cold maceration. Physicochemical studies of powdered crude drugs, preliminary phytochemical analysis, heavy metal analysis for arsenic, lead, cadmium and mercury, test for aflatoxin B1, G1, B2, G2 and microbial analysis were carried out in both ethanolic plant extracts. The chromatographic fingerprints of ethanolic extracts of plants were recorded using HPTLC and the solvent system used was chloroform and methanol in the ratio 9:1. Results: The outcomes of preliminary phytochemical analysis revealed the presence of alkaloids, flavonoids, phenols, glycosides and steroids in both the plant extracts. Proteins, saponins and tannins were absent. Analysis of heavy metals and aflatoxins were Below Quantification Limits (BQL). Microbes E. coli, Salmonella, Staphylococcus aureus, and Pseudomonas aeruginosa, were absent. Total aerobic bacterial count and yeast mould count were well within the acceptable limits. The results obtained from HPTLC fingerprint lane analysis showed 12 and13 bands at wavelengths 254nm and 366nm respectively. Conclusion: The present study offers valuable information and act as a tool that can applied in standardization of ethanolic plant extracts of Vitex negundo and Opuntia dillenii.

Acute oral toxicity assessment of ethanolic extract of Cassia auriculata linn whole plant in Albino mice

The current study was conducted to assess the safety of an ethanol extract of the Cassia auriculata linn entire plant by detecting its potential toxicity following acute administration in Swiss albino mice. For the acute investigation, an ethanol extract of Cassia auriculata linn entire plant was supplied to albino mice in a single dose of 0-2000 mg/kg via feeding. After feeding, behavioral changes, adverse effects, and death were assessed for up to 14 days. Histopathological examinations were performed 14 days following oral dosing. At the end of the observation period, animals were sacrificed. In an acute investigation with ethanol extract of Cassia auriculata linn, the whole plant showed no behavioral alterations indicating detrimental effects or deaths. There were no significant differences in organ weights or gross necropsy between the treatment and control groups. The ethanol extract of Cassia auriculata linn entire plant can be deemed relatively harmless at the oral dose tested because it did not induce any mortality or unfavorable behavioral changes in albino mice, according to a necropsy investigation in acute toxicity.

Phytochemical Screening and Antibacterial Potency of Aspilia africana Leaves and Gardenia jasminoides Root Extracts

The increasing resistance of pathogenic bacteria to conventional antibiotics has necessitated the search for alternative antimicrobial agents, particularly from plants. This study investigates the phytochemical composition and antibacterial activity of ethanol and n-hexane extracts of Aspilia africana leaves and Gardenia jasminoides roots. Phytochemical screening was carried out using standard methods. The extracts were tested against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis using the disc diffusion method and Minimum Inhibitory Concentration (MIC) determination. The phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, and terpenoids in varying concentrations across both plant extracts. The ethanol extract of Aspilia africana exhibited the highest flavonoid content (12.38%), while Gardenia jasminoides roots had a significantly higher tannin concentration (23.68%) in the ethanol extract compared to Aspilia africana (0.12%). Alkaloids and saponins were also abundant in Gardenia jasminoides roots (5.37% and 9.85%, respectively), particularly in the ethanol extract. Both plant extracts demonstrated significant antibacterial activity, with the ethanol extracts showing higher potency. The results of the antimicrobial analysis showed that the ethanol extracts of both plants demonstrated greater antimicrobial efficacy, particularly at higher concentrations. Gardenia jasminoides root extract showed inhibitory activity against all tested bacteria at 50mg/l and lower concentrations. Similarly, the n-hexane extract exhibited antibacterial activity, albeit less potent than the ethanol extract. In conclusion, the results indicate that both plant species possess potent antibacterial properties, particularly in ethanol extracts, supporting their traditional use in treating bacterial infections and justifying further research into their medicinal applications.

An Insilco approach on Trachyspermum Ammi to Discover a Drug that Reduces the Burden of Gallstone and Gallbladder Tumor

Nowadays, after pandemic circumstances peoples from developing countries and in particular Indians have faith towards herbal medicine to treat chronic disorders. Based on the previous literature it is stated that Indians has genetic impact for the formation of gallstones in liver. The mutation on a single gene in the liver may leads to liver malfunctions and stone formation. In Tamil Nadu most commonly peoples were affected with two types of stone with the major compositions of bilirubin, cholesterol, and calcium with 26%, 7%, and 8 % respectively. Initially gallstone is asymptomatic under medical diagnosis; the burden of disease increases day-by-day prolonged irritation of gall stone in gall bladder may leads to cancer. The mortality rate increases as the disease progresses. Hence, peoples are in need of alternate medicine to treat gall stone initially. In this regard, peoples of south part of India believe herbal medicine to treat and reduce the burden without any side effect. Based on the above, we made an attempt to find the bioactive compounds from Trachyspermum ammi (Ajwain seeds) using ethanol, petroleum ether and water for extraction. Gas chromatography-mass spectroscopy was used to analyze the plant's ethanol extract (GC-MS). Out of the thirty compounds that were chosen based on retention time, five aromatic functionally active compounds underwent docking studies using the bicyclo [3.1.0] of the tumor suppressor protein's SMAD4 receptor. hexan-2-ol, oleyl alcohol, trifluoroacetate, 3-cyclohexen-1-ol, 4-methyl-1-(1-methylethyl)-, thymol, phosphinoline, 1,2,3,4-tetrahydro-. The Insilco studies with protein-drug interaction significantly interact with 10 different proteins as a cascade process for biological changes. The result of the present study concluded that the extract has anti-inflammatory, antimicrobial, antioxidant, anticancer activity, specifically for gallbladder.

Acute Toxicity and In vivo Antidiarrhoeal Activity of Ethanol Extract and Fractions of Ipomoea triloba by Model Infection and Protection Tests in Mice against Selected Clinical Diarrhoeagenic Bacterial Isolates

Aim: This research work was aimed at investigating the acute toxicity and in vivo antidiarrhoeal activity of the extracts and fractions of Ipomoea triloba, a plant used locally for the treatment of acute infectious diarrhoea in order to investigate its safety profile. Study Design: 20 diarrhoeaic Clinical stool samples were collected and implicating organisms were isolated. Place and Duration of Study: Department of Pharmaceutical Microbiology and Biotechnology, University of Uyo AkwaIbom State, South-South Nigeria between May 2021-December, 2022. Methodology: Antibiogram, Plasmid curing and Intraperitoneal assay of acute toxicity and mouse protection test of the ethanol extract of plant were carried out on the bacterial-diarrhoeaic isolates. Results: Antibiogram by the agar-diffusion technique indicated a predominant resistance by the isolates. Plasmid curing assessment for antidiarrhoeal activity indicated a predominantly plasmid-borne resistance. Combined antidiarrhoeal activity of the ethanol extract and fractions of Ipomoea triloba with standard antibiotic (tetracycline), assessed by the activity index profile (AIP), showed a reduction in the in antagonistic activity and an increase in protective efficacy after plasmid curing. Acute toxicity (LD50) assay in albino mice (15.0-18-0 g body weight) indicated that the ethanol extract of Ipomoea triloba showed relative non-toxicity with an estimated value of 946.68 mg/kg body weight. In vivo antidiarrhoeal activity and protection efficacy of the ethanol extract assayed by mouse protection test (MPT) infection model offered appreciable (50.0 - 83.3%) protection to the bacterial-diarrhoeaic isolates challenged mice, compared with the relatively moderate (33.3 - 50.0%) protection by the control. Conclusion: There is need for further studies to elucidate the active principles responsible for the antidiarrhoeal activity of Ipomoea triloba in order to establish its structure activity relationship, this then can be introduced into the treatment regimen as a plausible remedy against acute hypersecretory infectious diarrhoea because of its safety profile which has been established in this work.

Isolation and characterization of Streptococcus agalactiae inducing mass mortalities in cultured Nile tilapia (Oreochromis niloticus) with trials for disease control using zinc oxide nanoparticles and ethanolic leaf extracts of some medicinal plants

BackgroundStreptococcus agalactiae (Group B streptococcus, GBS) induces a serious infection that can harm not only aquatic life but also humans and other animals. In a fish farm in southern Egypt, Nile tilapia (Oreochromis niloticus) has developed an epidemic with clinical symptoms resembling piscine streptococcosis.ResultsInitial microscopic inspection of the affected fish brain and kidney indicated the presence of Gram-positive cocci. S. agalactiae was effectively isolated and identified using nucleotide homology of the 16S rRNA and species-specific PCR. The partial 16S rRNA sequence was deposited in the GenBank database at the NCBI and given the accession number MW599202. Genotyping using RAPD analysis indicated that the isolates in the present study belonged to the same genotypes and had the same origin. The challenge test, via immersion (9.2 × 107, 9.2 × 106, and 9.2 × 105 CFU/ml for 1 h) or intraperitoneal injection (4.6 × 107, 4.6 × 106, and 4.6 × 105 CFU/fish), elicited clinical symptoms resembling those of naturally infected fish with a mortality rate as high as 80%. The ability to create a biofilm as one of the pathogen virulence factors was verified. Zinc oxide nanoparticles and the ethanolic leaf extracts of nine medicinal plants demonstrated considerable antibacterial activities against the tested S. agalactiae strain with low minimum bactericidal concentrations (MBC) and minimum inhibitory concentrations (MIC). The ethanolic leaf extracts from Lantana camara and Aberia caffra showed potent antibacterial activity with MBC values of 0.24 and 0.485 mg/ml, and MIC values of 0.12 & 0.24 mg/ml, respectively.ConclusionThis study isolated S. agalactiae from O. niloticus mortalities in a fish farm in Assiut, Egypt. The pathogen persists in fish environments and can escape through biofilm formation, suggesting it cannot be easily eliminated. However, promising findings were obtained with in vitro control employing zinc oxide nanoparticles and medicinal plant extracts. Nevertheless further in vivo research is needed.

Protective effect of evodiamine on acetic acid-induced gastric ulcers in rats through regulation of ROS/ICAM1/Nrf2 signaling pathway

Purpose: To investigate the anti-inflammatory and antiprotozoal effects as well as chemical profile of ethanol extracts of Hedyotis diffusa (Rubiaceae) (HDE) and Scutellaria barbata (Lamiaceae) (SBE). Methods: Dried whole plants collected in Vietnam were extracted with ethanol (30 and 96 %) by maceration for 4 weeks. The resulting extracts obtained after evaporating the solvent were stored in a refrigerator at 4 oC. Mouse carrageenan-induced inflammation and mouse macrophage cell line RAW264.7 were used to assess the in vivo and in vitro anti-inflammatory activities, respectively, while ciliate, Tetrahymena pyriformis was used to determine antiprotozoal effects. Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was employed to analyze the chemical profiles of the extracts. Results: All the extracts manifested anti-inflammatory effects in vitro and in vivo. The HDE exhibited significantly higher in vitro anti-inflammatory activity than SBE (p &lt; 0.05). Furthermore, in vivo antiinflammatory activity was higher in SBE compared to HDE. Both extracts exhibited antiprotozoal effects. The 96 % ethanol extract of both plants were more active than the 30 % ethanol extracts. Analysis by UHPLC‐Q‐TOF- MS revealed the presence of chrysin, apigenin and apigenin derivatives, naringenin, wogonin, quercetin and quercetin derivatives, as well as scutellarin. Conclusion: The HDE and SBE extracts from Vietnam exhibit significant in vivo and in vitro antiinflammatory activities and in addition, antiprotozoal activity against Tetrahymena pyriformis. These plants, therefore, are potential sources of antiprotozoal agents.

Phytochemical and pharmacological evaluation of bacopa monniera for anti – anxiety activity

Anxiety, a common emotional response to stress, involves numerous brain regions and neurotransmitters, including the amygdala, prefrontal cortex, and hippocampus, and is partially by genetic, environmental, and biological factors. This study evaluates Bacopa monnieri, a herb known in Ayurvedic medicine for its cognitive-enhancing and adaptogenic properties, as a potential anti-anxiety agent. The plant's ethanolic extract revealed a variety of phytochemicals, including alkaloids, flavonoids, and saponins. Acute toxicity studies indicated a high safety margin with an LD50 exceeding 2000 mg/kg body weight. The anti-anxiety effects were assessed using three established models. In the Elevated Plus-Maze Test, Bacopa monnieri significantly reduced anxiety levels in mice, comparable to the standard anxiolytic benzodiazepine. The Hole-Board Test showed increased head-dipping and rearing behaviors, while reducing immobility, suggesting anxiolytic activity. The Light-Dark Exploration Test demonstrated the herb's ability to decrease anxiety-related behaviors, including a reduction in rearing in dark compartments and increased exploration of light areas. These findings provide scientific validation for Bacopa monnieri's traditional use in treating anxiety and support its potential as an effective and safe anti-anxiety agent in managing central nervous system disorders.

Determination of insecticidal potential of selected plant extracts against fall armyworm (Spodoptera frugiperda) larvae

The fall armyworm, Spodoptera frugiperda (J.E. Smith), is a devastating pest that attacks a wide range of crops, including sugarcane, rice, and maize. The purpose of this study is to evaluate the toxicity potential of native plant extracts (Azadirachta indica, Eucalyptus globulus, Parthenium hysterophorus, Cannabis sativa, Citrullus colocynthis, Nicotiana tabacum) against S. frugiperda. Four different concentrations (50, 100, 200, and 400 ppm) of the ethanolic plant extracts was evaluated against S. frugiperda third-instar larvae to determine their median lethal concentration (LC50). After 72 h of exposure, the LC50 values of A. indica, E. globulus, P. hysterophorus, C. sativa, C. colocynthis, N. tabacum, and positive control (Spinetoram) were 186.104, 518.438, 320.027, 334.259, 252.651, 720.980 and 189.369 ppm respectively. The maximum percent mortality was caused by the highest concentration (400 ppm) of A. indica (64 ± 0.18), E. globulus (48 ± 0.22), P. hysterophorus (56 ± 0.18), C. sativa (56 ± 0.18), C. colocynthis (60 ± 0.00), and N. tabacum (40 ± 0.28), after 72 h of treatment while Spinetoram induced 100 ± 0.00 percent mortality of S. frugiperda and only 4 ± 0.18 percent mortality was recorded in a control group. Results showed that all plant extracts were found to be effective against S. frugiperda. The compounds from the two most effective ethanolic plant extracts were identified by using Gas chromatography-mass spectrometry analysis (GC-MS). The key compounds identified in neem leaf extract and kortuma fruit extract are predominantly biologically active molecules. Many of them were volatile compounds that belonged to different chemical categories, such as fatty acids, hydrocarbons, esters, terpenoids, phenolic compounds, and amines. Terpenes exhibited a wide range of different biological activities, such as serving as insecticides and antifeedant. The presence of various functional groups in the plant extract was determined by conducting a Fourier Transform Infrared Spectroscopy (FTIR). Farmers should employ these kinds of environmental friendly insecticides to lessen the impact of fall armyworm because these products are cheaper to use and better for the economy and the environment.

Antidiabetic and Antihyperlipidemic Activity of Ethanolic Extract of Mentha viridis in Alloxan Induced Diabetic Rats

This research was designed to examine the phytochemicals of Mentha viridis (M. viridis) ethanolic extract and the antidiabetic and antihyperlipidemic activities in alloxan-induced animal models. Diabetes was induced chemically by administering a unit dose of alloxan at 120 mg/kg BW. After alloxan induction, hyperglycemic rats were dealt with ethanolic extract of leaf and whole plant, metformin, and a mixture of leaf extract with metformin and whole plant extract with metformin for two weeks. Ethanolic extract of leaf and whole plant, metformin, and a combination of both leaf and whole plant extract with metformin therapies reduced glucose levels in the blood compared with the diabetic negative control group after two weeks of treatment. However, among the therapies, the ethanolic leaf extract and the combination of whole plant extracts with metformin were found to be the most effective (p&amp;lt;0.05), with reductions of 62.82% and 72.89%, respectively. After diabetes induction, the serum level of TG (triglycerides), TC (total cholesterol), LDL-C (low-density lipoprotein-cholesterol) escalated notably (p&amp;lt;0.05), and HDL-C (high-density lipoprotein-cholesterol) level decreased remarkably (p&amp;lt;0.05) in hyperglycemic rats as opposed to healthy normal rats. Ethanolic leaf extract and a combination of whole plant extract with metformin significantly minimized the elevated extent of TG and LDL-C. They surged HDL-C, but the TC level was reduced by whole plant extract only after two weeks of treatment. The standard procedures were used to identify the phytochemical compounds of the medicinal plant M. viridis. The phytochemical compounds such as alkaloids, resins, tannins, phenols, flavonoids, steroids, and terpenoids appeared in the ethanolic leaf extract of M. viridis. The findings suggest that M. viridis might provide better glycemic control and hypolipidemic effect in diabetic rats when administered alone or combined with oral antidiabetic agents. Incorporating M. viridis extract with metformin in improving hyperglycemic and hyperlipidemic conditions in diabetic rats proves that M. viridis has a synergistic effect, which could enhance the antidiabetic activity of oral hypoglycemic agents.

In-vitro Antifungal Activity of Ethanol Plant Extracts of Moringa oleifera, Vernonia amygdalina and Ocimum gratissimum against Some Clinical Candida Species

In-vitro Antifungal Activity of Ethanol Plant Extracts of Moringa oleifera, Vernonia amygdalina and Ocimum gratissimum against Some Clinical Candida Species

Discovery of Active Antibacterial Fractions of Different Plant Part Extracts of clove (Syzigium aromaticum) Against Streptococcus mutans

Nowadays, dental cavities caused by Streptococcus mutans are a major focus of research in Indonesia. While several antibiotics are available to combat this bacterium, concerns about antibiotic resistance have prompted researchers to explore natural remedies. Clove (Syzigium aromaticum) is a commonly studied natural remedy against dental cavities and S. mutans. Among the different parts of the clove plant, clove bud is the most widely used against dental cavities or S. mutans, and the potential of other clove parts has not been thoroughly explored. Identifying which parts of the clove plant have higher concentrations of active ingredients and exhibit the strongest antibacterial activity is important. Therefore, this study evaluated the antibacterial activity of three different parts, i.e., leaf, stems, and buds of the clove plant ethanolic extracts against S. mutans. The ethanolic extracts of clove leaf, stems, and buds were prepared using the maceration method with 70% ethanol, and their activity against S. mutans was tested using the disc diffusion method at three different concentrations (10%, 5%, 2.5% b/v). Fractionation was carried out using hexane and water to obtain two fractions: hexane and water fraction. These fractions were then subjected to antibacterial assays. The ethanolic leaf, stems, and bud extracts exhibited varying antibacterial activity levels. The best activity was observed with the 10% clove bud ethanolic extract, which produced an inhibition zone of 20.83 ± 0.77 mm. The leaf and stem extracts showed inhibition zones of 16.38 ± 3.84 mm and 17.95 ± 5.15 mm, respectively. Furthermore, the hexane-soluble fraction of the clove bud displayed the highest activity with an inhibition zone diameter of 23.7 ± 3.21 mm at 10%. This activity was twice as high as ampicillin, used as the positive control. In conclusion, clove bud remains the best source of antibacterial compounds against S. mutans. Fractionation of the bud extract using hexane can significantly enhance its activity. Further investigation should be conducted to optimize the effectiveness of this active fraction for use as an anti-dental caries treatment.

Antilipidemic Effect of Ethanolic Extract of Aloe barbadensis Gel, Cymbopogon citratus Leaves and Its Combination on Alloxan Induced Diabetic Rats

This research sought to investigate the antilipidemic effect of ethanolic extract of Aloe barbadensis, Cymbopogon citratus leaves and its equal combination on induced-alloxan diabetic rat. Plants like Aloe barbadensis and Cymbopogon citratus are commonly used as home remedies for some diseases such as diabetes thereby making them alternative medicines. Ethanolic extract of both plants was extracted using 70% ethanol. The effect of glibenclamide, ethanolic extract of A. barbadensis gel, C. citratus leaves and its combination after 21 days experimental study showed a significantly increased (P&lt;0.05) high density lipoprotein (HDL) in the induced rats when in comparison with the normal control and diabetic untreated rats. The low density lipoprotein (LDL) significantly showed a (P&lt;0.05) decrease in the rats treated with A. barbadensis gel, C. citratus leaves and its combined extract compared with those treated with standard drug. The serum total cholesterol (TCHOL), triglyceride (TG) and very low density lipoprotein (VLDL) significantly showed a (P&lt;0.05) decrease in the rats treated with A. barbadensis gel, C. citratus leaves and its combined extract compared with diabetic untreated rats. This research revealed that ethanolic extract of A. barbadensis, C. citratus and its combination has antilipidemic effects and holds potential as an alternative therapy for its management.

In vivo and in silico elucidation of possible potential and mechanisms involved in the analgesic action of ethanolic extract of Lavandula Stoechas.

Our research focused on plant's ethanolic extract Lavandula stoechas flower part to investigate the potential analgesic effects and possible pathways involvements. Four experimental tests were performed on Swiss albino mice with five animals in each group at different doses (50, 100, and 200mg/kg); formalin test, tail-flick test, acetic acid-induced writhing, and hot-plate test. The opioidergic, noradrenergic, cholinergic, and K channel blockers in the analgesic actions were also carried out for the potential route involvement. The percentage inhibition for abdominal writhing's and formalin activity showed a dose-dependent manner for early and late phases reducing abdominal writhing's and time period of licking, respectively. Tail immersion and hot-plate test demonstrated a substantial and dose-dependent increase in the latency time and time period of paw liking and jumping response respectively. GC-MS showed the abundantly present compounds were octadecatrienoic acid (34.35%), n-hexadecanoic acid (12.98%). In silico analyses have revealed three compounds that had good interactions with 6y3c receptor proteins, demonstrating strong binding affinities and satisfying docking parameters. Overall, these studies showed that ethanolic extract of L. stoechas is an important medicinal plant, with both central and peripheral antinociceptive and analgesic activities supporting its traditional use for therapeutic purposes.

Larvicidal activity of Sauromatum venosum tuber extract against Culex spp.

Mosquitoes act as the vectors of a variety of diseases that have serious health consequences for humans. In order to stop the spread of disease through mosquitoes, plant extracts have been used as a secure and environmentally friendly alternative to chemical pesticides. Plants produce secondary metabolites that inhibit the growth of insects and exhibit larvicidal activities. In the current study, the Bioefficacy of ethanolic extract of Sauromatum venosum was tested against the fourth instar larva of Culex spp. The LC50 value of ethanolic extract of Sauromatum venosum was 55.571 ppm. The mortality of larvae was examined after 96hours of exposure. The results revealed the promising potential larvicidal properties of the ethanolic extracts of Sauromatum venosum plant against the fourth instar larvae of Culex spp. It has been concluded from this research that larvicidal activities of ethanolic extracts of Sauromatum venosum are due to the presence of phytochemical constituents in it which were confirmed by FTIR spectrum.