Ethanol Extraction Process Research Articles

Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour.

Amomum villosum Lour. (A. villosum), known as Sharen in China, is widely used for culinary and medicinal purposes due to containing a diverse set of bioactive compounds. In this study, the optimum ethanol extraction process was optimized and the composition and biological activities (antioxidant and antitumor) of five different fractions (dichloromethane, petroleum ether, ethyl acetate, n-butanol and H2O) extracted from the ethanol extract of A. villosum were investigated. The results showed that the optimal extraction conditions were extraction temperature 80°C, extraction time 120 min, ethanol concentration 40% and solid-liquid ratio 1:25 g/mL. Moreover, 35 bioactive compounds were successfully identified by UPLC-ESI-QTOF-MS/MS from five factions for the first time, including 12 phenolic acids and derivatives, 2 organic acids, 12 flavonoids and derivatives, 2 oxylipins and 7 proanthocyanidins. Among them, ethyl acetate fraction (Fr-EtOAc) exhibited the highest content of total phenolic (374.01 mg GAE/g DW) and flavonoid (93.11 mg RE/g DW), where vanillic acid, catechin, epicatechin and protocatechuic acid were the predominant phenolic compounds that accounting for 81.65% of the quantified bioactive compounds. In addition, Fr-EtOAc demonstrated excellent total antioxidant activity (IC50 of DPPH and ABTS assays were 0.23, 0.08 mg/mL, respectively, and FRAP assay was 322.91 mg VCE/100 g DW) and antitumor activity (1,000 μg/mL, 79.04% inhibition rate). The results could provide guidance for the industrial production and application of A. villosum.

Optimization of tetrastigma hemsleyanum extraction process based on GA-BPNN model and analysis of its antioxidant effect

Tetrastigma hemsleyanum (Tetrastigma hemsleyanum Diels et Gilg) is a valuable traditional Chinese medicine with various applications. In this study, we aimed to optimize the extraction process for the total extraction yield of five flavonoid components, namely kaempferol, quercetin, rutin, kaempferol-3-O-rutinoside, and astragalin from the Tetrastigma hemsleyanum root (THR), and explore its potential molecular mechanisms in treating oxidative diseases as well as antioxidant activity. To achieve these objectives, we employed the genetic algorithm-back propagation neural network (GA-BPNN), the Box-Behnken design (BBD) with 4-factors and 3-levels to establish the optimal ethanol extraction process for the total extraction yield of the 5 components. Using public databases, the “component core targets-disease core target genes” networks were built, as well as molecular docking. Furthermore, DPPH was used to examine the antioxidant activity of the extracts obtained from THR under the optimal extraction process. The experimental value of the total extraction yield of the 5 components achieved a maximum of 788.12 mg/kg when the ethanol concentration was 73%, the solid-liquid ratio was 26 g/mL, and the ultrasonic duration was 30 min, and the ultrasonic temperature was 76 °C. When docked with protein molecules such as 6Y8I, quercetin, and other components received moderate to high scores. When the total concentration of the 5 components was 3.033 μg/mL, the DPPH radical scavenging rate was 89.81%. Compared with the BBD method, the GA-BPNN method is more efficient and reliable for optimizing the extraction process of active ingredients in THR because of its good data-fitting ability.

Extraction, Purification, and Component Identification of Monoterpene Glycosides from Paeonia suffruticosa Seed Meal.

Paeonia suffruticosa (P. suffruticosa) seed meal is a byproduct of P. suffruticosa seed processing, which contains bioactive substances such as monoterpene glycosides, and has not been effectively utilized at present. In this study, monoterpene glycosides were extracted from P. suffruticosa seed meal using an ultrasound-assisted ethanol extraction process. The monoterpene glycoside extract was then purified by macroporous resin and identified using HPLC-Q-TOF-MS/MS. The results indicated the following optimal extraction conditions: ethanol concentration, 33%; ultrasound temperature, 55 °C; ultrasound power, 400 W; liquid-material ratio, 33:1; and ultrasound time, 44 min. Under these conditions, the yield of monoterpene glycosides was 121.03 mg/g. The purity of the monoterpene glycosides increased from 20.5% (crude extract) to 71.2% (purified extract) when using LSA-900C macroporous resin. Six monoterpene glycosides (oxy paeoniflorin, isomaltose paeoniflorin, albiflorin, 6'-O-β-D-glucopyranoside albiflorin, paeoniflorin, and Mudanpioside i) were identified from the extract using HPLC-Q-TOF-MS/MS. The main substances were albiflorin and paeoniflorin, and the contents were 15.24 mg/g and 14.12 mg/g, respectively. The results of this study can provide a theoretical basis for the effective utilization of P. suffruticosa seed meal.

Optimization of ethanol reflux extraction process of Ziziphi Spinosae Semen- Schisandrae Sphenantherae Fructus based on network pharmacology combined with response surface methodology

The present study optimized the ethanol extraction process of Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus drug pair by network pharmacology and Box-Behnken method. Network pharmacology and molecular docking were used to screen out and verify the potential active components of Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus, and the process evaluation indexes were determined in light of the components of the content determination under Ziziphi Spinosae Semen and Schisandrae Sphenantherae Fructus in the Chinese Pharmacopoeia(2020 edition). The analytic hierarchy process(AHP) was used to determine the weight coefficient of each component, and the comprehensive score was calculated as the process evaluation index. The ethanol extraction process of Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus was optimized by the Box-Behnken method. The core components of the Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus drug pair were screened out as spinosin, jujuboside A, jujuboside B, schisandrin, schisandrol, schisandrin A, and schisandrin B. The optimal extraction conditions obtained by using the Box-Behnken method were listed below: extraction time of 90 min, ethanol volume fraction of 85%, and two times of extraction. Through network pharmacology and molecular docking, the process evaluation indexes were determined, and the optimized process was stable, which could provide an experimental basis for the production of preparations containing Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus.

Optimization of ultrasound-assisted extraction method for phytochemical compounds and antioxidant activities of sour jujube extracts.

Ultrasonic-assisted extraction is a rapid and effective extraction method that uses ultrasound energy and solvents to extract target compounds from various plant matrices. In this study, the ultrasonic-assisted extraction conditions of sour jujube were optimized. A five-level central composite design (CCD) with four variables was used to evaluate ultrasonic treatment variables influencing the total saponin content (TSC), total flavonoid content (TFC), and total phenolic content (TPC) extracted from sour jujube. The solvent concentration, extraction time, ultrasonic power, and solid-to-liquid (S/L) ratio were optimized using aqueous ethanol and methanol solutions as extraction solvents. A central composite design (CCD) was used for an in-depth study, and then the optimal value that could produce the maximum TPC, TFC, TSC, and four in vitro antioxidant activities (scavenging activity of hydroxyl free radicals, ferric-reducing antioxidant power (FRAP), phosphomolybdic acid reduction method, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity) was determined. Hydrogen peroxide-induced oxidative stress experiment confirmed that the Jujube extract could have an antioxidant role in vivo. The relationship between the contents of three compounds and the antioxidant activity in vitro and in vivo was further studied. The results showed that optimizing methanol and ethanol extraction process parameters could improve target components' extraction efficiency. Under the optimum conditions, the TFC and TPC yields of sour jujube by ethanol are better than methanol, while the yield of TSC by methanol is better than ethanol. In vivo data showed that Jujube extract protects against the adverse effects of oxidative stress and improves the life span of female and male Drosophila. This study provides a valuable reference for the full use of Ziziphus jujube, as well as a new direction in food development.

Biomineralization behavior of ternary mesoporous bioactive glasses stabilized through ethanol extraction process

The development of bioactive implantable materials with multi-functional properties like tissue regeneration, tumor annihilation, anti-bacterial growth and angiogenesis advancement is of great importance. In this context, mesoporous bioactive glasses (MBGs) are gaining tremendous interest in designing the next generation of biomaterials for the bone defect treatment. In this work, ternary SiO2-CaO-P2O5 MBGs have been synthesized by using the acid assisted sol-gel process. In contrast to the conventional process, we adopted an ethanol extraction process to remove surfactant, leading to superior textual properties and high silanol group density in resultant bioglass. Magic angle spinning nuclear magnetic resonance (MAS-NMR) technique has been used to elucidate the presence of different anionic species in the pristine glass samples and its variation with chemical compositions. The vibrational spectroscopy reveals the presence of high concentration of silanol group over the surface of pristine glass samples, which effectively accelerates the formation of hydroxyl carbonate apatite (HCA) layer. The MBG specimens show a good cell viability behavior without toxicity up to the concentration of 20 µg ml−1. In the present results, we observed that pore size along with surface area and silanol group density play an effective role in the growth of HCA layer.

EFFICACY OF NIGERIAN GROWN Erythrophleum suaveolens (Gull. And Perr.) Brenan BARK EXTRACTS AS BIOPRESERVATIVE AGAINST TERMITE ATTACK

Many synthetic chemicals that are used to protect wood from biodeterioration are associated with environmental pollution and toxicity to human beings. As a part of the search for less hazardous preservatives, extracts from the bark of Erythrophleum suaveolens were evaluated using ethanol extraction process. Samples of barks of Erythrophleum suaveolens were obtained from Oyo town, Nigeria. Phytochemical and elemental analyses were carried out on the oil extracts. Permeability and decay resistance tests were carried out on lumber samples of Ceiba pentandra in accordance with ASTM standards. Results were analyzed using Analysis of Variance (ANOVA) at 5% probability level. Phytochemical analysis indicated the presence of Saponin (13.56%), Tannin (29.71mg/g), alkaloid (6.39%) and Total Phenol (38.71mg/g) while elemental analysis showed the presence of Copper, Iron, Lead, Magnesium and Calcium respectively. Grave yard Test showed that the untreated Ceiba pentandra samples had the highest weight loss after 6 weeks of exposure while 100% treatment had least weight loss. The oil extract from bark of Erythrophleum suaveolens was effective against termite attack on Ceiba pentandra wood samples and therefore has good potentials as biological preservative for wood and other lignocellulosic materials.

Light-colored lignin isolated from poplar by ultrasound-assisted ethanol extraction: Structural features and anti-ultraviolet and anti-oxidation activities

Ethanol extraction of lignocellulosic biomass under mild conditions has re-attracted considerable attention since a light-colored lignin with low molecular weight can be obtained. Unfortunately, the yield of isolated lignin for the ethanol extraction process is very limited. To enhance the extraction efficiency of lignin from poplar, ultrasound was used to assist the ethanol extraction and the isolated lignin was analyzed in terms of structure features and functional properties. As expected, increasing the ultrasonic power and ultrasonic time led to higher lignin yields during the ultrasound-assisted ethanol extraction (UAEE) process. The chemical structure of the isolated lignin (UAE-L) was almost completely retained, the C-O (mainly β-O-4) linkages in the lignin only exhibited a slight decrease, while the C-C (β-β, β-5, and β-1) linkages were nearly unchanged. The UAE-L was light in color and had more phenolic hydroxyl groups, less carbohydrate impurities, lower molecular weight and narrower polydispersity as compared to the poplar MWL. The UAE-L could scavenge more 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH·), displaying a stronger antioxidant activity. Moreover, the UAE-L showed good UV-absorbing properties in both UVA and UVB regions. These results indicated that the UAE-L has great potential for replacing the commercial synthesized antioxidant and anti-UV ingredients.

Five Constituents Contributed to the Psoraleae Fructus-Induced Hepatotoxicity via Mitochondrial Dysfunction and Apoptosis.

Backgrounds: Psoraleae Fructus (PF)-induced hepatotoxicity has been reported in clinical and animal experiments. However, the hepatotoxic constituents and mechanisms underlying PF-induced toxicity have remained unclear. Therefore, this study explored the potentially toxic PF components and revealed their relative mechanisms. Methods: The hepatotoxicity of PF water (PFW) and ethanol (PFE) extracts was compared using Kunming mice. The different compositions between PFW and PFE, which were considered toxic compositions, were identified using the UHPLC-Q-Exactive MS method. Then, L02 and HepG2 cell lines were used to evaluate the toxicity of these compositions. Cell viability and apoptosis were determined through the Cell Counting Kit-8 (CCK-8) assay and flow cytometry, respectively. An automatic biochemical analyzer detected the aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Lastly, we used high-content screening (HCS) to determine the levels of reactive oxygen species (ROS), lipid, and mitochondrial membrane potential (MMP). Results: The ethanol extraction process aggravated the hepatotoxicity of PF, causing more severe injuries. The content of psoralen, isopsoralen, bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol was higher in the PFE than PFW. Bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol induced cell apoptosis and the AST, ALT, and ALP leakages. Furthermore, these five constituents increased intracellular lipid accumulation and ROS levels but decreased the MMP level. Conclusion: The ethanol extraction process could induce severe PF hepatotoxicity. Bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol are the main hepatotoxic ingredients. This mechanism could be associated with oxidative stress and mitochondrial damage-mediated apoptosis. Taken together, this study provides a basis for the clinical application of PF that formulates and improves its herbal standards.

Red Quinoa Bran Extract Prevented Alcoholic Fatty Liver Disease via Increasing Antioxidative System and Repressing Fatty Acid Synthesis Factors in Mice Fed Alcohol Liquid Diet.

Alcohol is metabolized in liver. Chronic alcohol abuse results in alcohol-induced fatty liver and liver injury. Red quinoa (Chenopodium formosanum) was a traditional staple food for Taiwanese aborigines. Red quinoa bran (RQB) included strong anti-oxidative and anti-inflammatory polyphenolic compounds, but it was usually regarded as the agricultural waste. Therefore, this study is to investigate the effect of water and ethanol extraction products of RQB on the prevention of liquid alcoholic diet-induced acute liver injury in mice. The mice were given whole grain powder of red quinoa (RQ-P), RQB ethanol extract (RQB-E), RQB water extract (RQB-W), and rutin orally for 6 weeks, respectively. The results indicated that RQB-E, RQB-W, and rutin decreased alcoholic diet-induced activities of aspartate aminotransferase and alanine aminotransferase, and the levels of serum triglyceride, total cholesterol, and hepatic triglyceride. Hematoxylin and eosin staining of liver tissues showed that RQB-E and RQB-W reduced lipid droplet accumulation and liver injury. However, ethanol extraction process can gain high rutin and antioxidative agents contents from red quinoa, that showed strong effects in preventing alcoholic fatty liver disease and liver injury via increasing superoxide dismutase/catalase antioxidative system and repressing the expressions of fatty acid synthesis enzyme acetyl-CoA carboxylase.

Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells

Abstract Glycosmis stenocarpa is a species of shrub found in the Northern provinces of Vietnam. Its roots contain different carbazolic derivatives, mainly Murrayafoline A (Mu-A), which exhibits valuable biological activities. In this study, we performed an extraction of Mu-A from the roots of G. stenocarpa and optimized this process using response surface methodology (RSM) according to a central composite design, with three independent parameters including extraction time (min), extraction temperature (°C), and solvent/material ratio (mL/g). Two dependent variables were the Mu-A content (mg/g raw materials) and extraction efficiency (%). The optimal conditions to extract Mu-A were found to be as follows: extraction temperature, 67°C; extraction time, 165 min; and solvent/material ratio, 5:1. Under these conditions, the Mu-A content and extraction efficiency were 38.94 ± 1.31 mg/g raw materials and 34.98 ± 1.18%, respectively. Mu-A exhibited antiproliferation and antitumor-promoting activity against the HepG-2 cell line. The present optimization work of Mu-A extraction from G. stenocarpa roots contributed to the attempt of designing a large-scale extraction process for the compound and further exploitation of its potential in vivo applications.

Multi-index optimization of extraction process of Fengyin Decoction based on BAS-GA-BP neural network combined with entropy weight method

To optimize the ethanol extraction technology parameters of Fengyin Decoction by orthogonal experiment combined with beetle antennae search(BAS)-genetic algorithm(GA)-back propagation neural network(BPNN). Based on single factor investigation, the extraction temperature, ethanol volume, extraction time, and ethanol concentration were used as orthogonal experiment factors, and entropy weight method was used to calculate the comprehensive scores of aloe-emodin, glycyrrhizic acid ammonium salt, rhein, emodin, chrysophanol, physcion, cinnamaldehyde, 6-gingerol, extraction ratio and fingerprint similarity. BAS-BPNN model was established, and then, GA was used to predict the optimal extraction process. The results showed that BAS-BPNN was optimized to obtain the optimal ethanol extraction process of Fengyin Decoction as follows: extraction temperature of 87 ℃, adding 9 times of 75 % ethanol, and extracting for 47 minutes, with a comprehensive score of 1.052 9. Meanwhile, the optimal process parameters obtained by orthogonal design were as follows: the extraction temperature of 80 ℃, adding 10 times of 75% ethanol, extracting for 30 minutes, with a comprehensive score of 1.003 7. The comprehensive score of the process obtained from the BAS-BPNN model was slightly better than that from the orthogonal test, indicating that the optimized process from BAS-BPNN model was more ideal, so it was finally determined as the best extraction process for Fengyin Decoction. The process of Fengyin Decoction obtained from BAS-GA-BPNN has high extraction efficiency and good stability, which provides reference for the subsequent development and quality control.

Consistency of Spectra and Antibacterial Activity of The Extract Mixture of Curcuma longa, Zingiber officinale, and Syzigium aromaticum

Finding new antibiotic based on local resources is always a challenging effort, since Indonesia almost totally import the antibiotics. Then, antibiotic based jamu is an alternative. Jamu is a traditional herb prepared from local plants and some rhizomes, such as Curcuma longa, Zingiber officinale, and Syzigium aromaticum. These plants have been reported for their antibacterial capability. This paper is focused on the evaluation of antibacterial of these mixtures and their spectral consistency. The method was initiated by extraction of each plants/rhizome using ethanol and water. Each extract was mixed with equal ratio, and further evaluation for growth inhibiting activity in Staphylococcus aureus and Escherichia coli. Meanwhile, the spectral analysis was determined using FTIR and UV-Vis spectrophotometry. The result showed that the mixture of ethanol extract gives a slightly better activity than that using water extract. Furthermore, the UV-Vis spectra of the mixture from ethanol extracts indicates different band absorption in 439 and 417 nm, but no absorption observed in water extract in this range. In addition, the ethanol mixture extract also gives new band (FTIR spectra) in between 1010-1045 cm−1, that these are not observed in water extract. In short, it can be summarized that ethanol extraction process give better extraction procedure and provide better antibacterial activity.

Processing of permeabilized Chlorella vulgaris biomass into lutein and protein-rich products

Chlorella vulgaris is rich in lutein, a high-value pigment known for its antioxidant properties. In this work, the effect of pulsed electric field (PEF) on enhancing the selective pigment extraction from fresh C. vulgaris was tested. PEF treatment enhanced the lutein (2.2 ± 0.1-fold) and chlorophyll yields (5.2 ± 3.4-fold) compared to non-treated cells when using a single-stage ethanol extraction process. A process simulation model was used to assess the viability of the pigment extraction process. The cost estimates showed that the PEF treatment reduced the processing costs and enhanced the economic feasibility of the process. The extraction of algal protein from lutein-depleted biomass was also simulated to determine the benefit of using low-cost microalgae meal for production of proteins concentrates. The cost estimates revealed that unless extraction yield and protein content of the concentrate were improved, the estimated protein cost (US$4.16 kg−1) would not be competitive to existing similar products from soybeans and peas.

Ethanol extraction technology of Epimedii Folium and protective effect of ethanol extract on chondrocyte

Uniform design-comprehensive scoring method was used to investigate the effects of ethanol dosage, ethanol concentration and extraction time, based on the evaluation index from transfer rates of epimedin A, epimedin B, epimedin C, icariin and baohuoside Ⅰ, which are the main active components in Epimedii Folium. Furthermore, the optimum conditions for the ethanol extraction process were determined by multiple linear stepwise regression and empirical test. Then, the ethanol extract of Epimedii Folium prepared according to the optimized technological conditions was used to intervene the injury model of chondrocyte induced by interleukin-1 beta(IL-1β). Annexin V-FITC/PI staining flow cytometry was used to detect the apoptotic rate of chondrocyte and analyze the effect of ethanol extract of Epimedii Folium on chondrocyte injury model. The optimum conditions of ethanol extraction were as follows. Crude powder of Epimedii Folium was added with 18 times of 70% ethanol solution, and extracted for twice in the refluxing process, for 60 minutes each time. Under the conditions, the extraction rates of the above five active components were 94.21%, 94.76%, 93.85%, 96.17% and 96.85%, respectively. The optimized ethanol extraction process of Epimedii Folium was reasonable, feasible and reproducible. This ethanol extract could significantly reduce the early apoptotic rate, late apoptotic and necrotic rate, total apoptotic rate(P<0.05 or P<0.01) of chondrocyte injury model induced by IL-1β, suggesting that the ethanol extract of Epimedii Folium can inhibit the apoptosis of chondrocytes induced by IL-1β to a certain extent, which lays a foundation for further study on its prevention and treatment of osteoarthritis.

Application of magnetic adsorbent with silicate and phenyl polymers to adsorb Rhodamine B

The extensive use of Rhodamine B (RhB) for textile, paper, pigment, food, cosmetic, and drug manufacturing and its indiscriminate disposal leads to serious human, biological, and environmental hazards. A magnetic adsorbent with silicate and phenyl polymers (Ph/SiO2/Fe3O4) has been prepared to absorb RhB. The morphology and structure of the adsorbents have been characterized by TGA, XRD, FTIR, and adsorption–desorption measurement. The results revealed that Ph/SiO2/Fe3O4 exhibited a paramagnetic behavior and could easily and quickly be separated from a suspension. The RhB adsorption behavior was almost pH independent due to the adsorption between the phenyl ring of Ph/SiO2/Fe3O4 and RhB by π–π electron-donor–acceptor interactions. The adsorption behavior of RhB adsorption was in good agreement with the Langmuir adsorption isotherm, and the maximum adsorption capacity was 142.186 mg g−1. Good desorption performance of Ph/SiO2/Fe3O4 showed that this novel magnetic adsorbent cannot only be activated by ethanol extraction process but also reuse by the recovery of magnetic force.

Multi-response Optimization for Unfertilized Corn Silk Extraction Against Phytochemical Contents and Bio-activities

This study was designed to optimize ethanol extraction process of unfertilized corn silk (UCS) to maximize phytochemical contents and bioactivities. The response surface methodology (RSM) with central composite design (CCD) was employed to obtain the optimal extraction conditions. The influence of ethanol concentration, extraction temperature and extraction time on total polyphenol contents, total flavonoid contents, maysin contents, 2,2-diphenyl-1- picrylhydrazyl(DPPH) radical scavenging activities and tyrosinase inhibition were analyzed. For all dependable variables, the most significant factor was ethanol concentration followed by extraction temperature and extraction time. The following optimum conditions were determined by simultaneous optimization of several responses with the Derringer’s desirability function using the numerical optimization function of the Design-Expert program: ethanol concentration 80.45%, extraction temperature 53.49°C, and extraction time 4.95 h. Under these conditions, the predicted values of total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity and tyrosinase inhibition were 2758.74 μg GAE/g dried sample, 1520.81 μg QUE/g dried sample, 810.26 mg/100g dried sample, 56.86% and 43.49%, respectively, and the overall desirability (D) was 0.74.

Optimize ethanol-extraction procedure of Shenlian granules by orthogonal design with pharmacodynamics index

To optimize the ethanol extraction process for Shenlian formula. On the basis of the pharmacodynamics index for different extraction process routes, the contents of salvianolic acid B, tanshinone ⅡA and berberine, as well as the extraction ratio in different experimental schemes were used as the ethanol extraction examining indexes, and multi-criterion synthesizing grading method was used for data analysis to optimize and verify the ethanol-extraction process conditions in orthogonal experiment. The optimum ethanol extraction process was as follows: adding 60% ethanol, 10 times amount, extracting for 2.0 h each time for a total of 2 times. This extraction process showed good stability and availability.

Adsorption of Rhodamine B dye by biomimetic mesoporous SiO2 nanosheets

Efficient treatment of solutions and solid wastes containing Rhodamine B (RB), a common dye with potential carcinogenicity and sometimes used illegally in food industry, is of great importance because inappropriate treatment may lead to serious human and environmental risks. A novel nanomaterial, the mesoporous silica nanosheets prepared via electrospinning and calcination are applied to the adsorption of RB from aqueous solutions in this work. This sheet-like material is superior to classic powder adsorbents in recoverability, while good availability and stability favor its practical application. The effects of key parameters such as system pH, stirring rate, initial concentration, and contact time are studied. The adsorption isotherm type and the kinetic characteristic are analyzed. The results show that the mesoporous SiO2 nanosheets are efficient in removing RB from aqueous phase, presenting great potential to become a practical dye adsorbent. Regeneration experiments show that this novel adsorbent can be activated by ethanol extraction process. This study provides the academic field with a successful application case of nanostructured materials, which can be applied to a wider range of dyes similar to RB in physicochemical properties, showing prosperous value in advanced environmental purification.

유산균 발효 배초향 추출물의 항산화 활성과 주름개선 효능

*Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon 200-701, Korea.**Department of Food Science and Engineering, Seowon University, Cheongju 361-742, Korea.ABSTRACT : This study was to investigate anti-skin wrinkle effect of Agastache rugosa Kentz extracts by extraction processes.In the comparison of the effect of the solvent extraction, the extracts by 70% ethanol (EE) showed better biological activitiesthat those by hot water. Therefore, further fermented Agastache rugosa was applied to 70% ethanol extraction process(FEE). FEE showed higher DPPH scavenging activity of 62.98% than EE’s 62.71% at 1.0 ㎎/㎖, but there was no significant.Elastase inhibition was measured 23.0% from FEE at 1.0㎎/㎖. Cytotoxicity showed the highest 16.26% from FEE, thisvalue is safe in the cell experiment. Collagen production showed 113.1 ng/ ㎖ from FEE, on the other hand EE was measured77.4 ng/㎖ in adding 1.0㎎/㎖. MMP-1 production was observed 1398 pg/㎖ from FEE and EE was measured1632 pg/㎖. These results were found the highest antioxidant and anti-wrinkle effect. As a result, it was also confirmed thatanti-skin wrinkle activities of the Agastache rugosa Kentz extract was correlated with anti-oxidant activities.Key Words : Anti-Skin Wrinkle Effect , Antioxidant, Agastache rugosa Kuntz, Collagen, Lactic Acid, MMP-1