In this study the effects of bilateral injections of cholinergic agents into the basolateral amygdala (BLA) region on ethanol‐induced conditioned place preference (CPP) were investigated in male Wistar rats. Subcutaneous (s.c.) administration of different doses of ethanol (0.25, 0.5, 1, 1.5 g/kg) produced a partial dose‐dependent CPP as (0.5 g/kg) of ethanol was effective dose. Using a 3‐day schedule of conditioning, it was found that intra‐BLA administration of the nicotinic receptor agonist, nicotine (0.25, 0.5, 0.75,1 and 1.5 ìg/rat) significantly potentiated the ethanol (0.5 g/kg)‐induced CPP. Moreover, intra‐BLA administration of the muscarinic receptor agonist, pilocarine (0.5, 1 and1.5 ìg/rat), potentiated the ethanol (0.5 g/kg)‐induced CPP. Furthermore, intra‐BLA administration of nicotinic receptor antagonist, mecamylamine (0.5, 0.75 and 1 ìg/rat) before an effective dose of nicotine (1 ìg/rat) and Subcutaneous (s.c.) of ethanol (0.5 g/kg) decreased the effect of nicotine‐induced potentiation of the ethanol response. On the other hand, intra‐BLA administration of the muscarinic receptor antagonist, atropine (1, 2, and 4 ìg/rat) inhibited the effect of pilocarpine‐induced potentiation of the ethanol CPP. Intra‐BLA injections of nicotine, mecamylamine, pilocarpine or atropine alone did not induce a significant place preference or place aversion.Tmu research Grant