The selective conversion of ethylene into propylene (ETP) has garnered significant attention due to the growing demand for the product of this reaction in the petrochemical industry. In this study, we developed a series of Ni-loaded dealuminated Beta zeolite catalysts for the ETP reaction. The catalyst loaded with 0.07 wt% Ni showed stable and high catalytic activity with 28% propylene yield and 16% butylene yield, despite very low Ni content compared to previously reported catalysts. Temperature-programmed H2 reduction and NH3-desorption analyses showed that the active sites were Ni species grafted onto the defective silanols of dealuminated zeolites.