This study evaluated under two contrasting field conditions, and during two seasons, four autochthonous species, Bromus rubens (Br), Brachypodium distachyon (Bd), Medicago truncatula (Mt) and Anthemis arvensis (Aa), as single species and in mixtures of Br + Mt, Br + Aa and Br + Mt + Aa, to determine their suitability as temporary cover crops (CCs) for Mediterranean woody crops. Br, Bd and Mt reached above 90 % ground cover (GC) in spring, while Aa reached a maximum of 80 %; in early winter GC varied from 0 % to 40 %, approximately, depending on the sowing date in the fall. The thermal time required to reach 30 % GC and seed maturity appeared as two key traits to evaluate the suitability of autochthonous species as temporary CCs for Mediterranean woody crops. Our experiment showed that early seeding (no later than mid-October) is critical to exploit these traits into effective CCs under Mediterranean conditions. Phenologic models developed for the tested species, based on temperature, are capable of predicting their development properly under field conditions, and they might be used to predict the evolution of GC when calibrated from field experiments. Aboveground biomass was affected by edaphic conditions, with higher values being observed in the most fertile soil, in Córdoba, in the range of 300–700 g m−2, compared to Adamuz with 150–350 g m−2. When used as monospecific CCs, Br, Bd and Mt had a higher aboveground biomass than Aa. We observed no differences in aboveground biomass among mixtures within each of the two locations. Differences in fine root biomass (RB) and fine root length density (RLd) resembled those of aboveground biomass, albeit with a higher variability. When used as monospecific CCs, fine RB and fine RLd were higher for Br and Bd, followed by Mt, with Aa having the lower values. Fine RB, or fine RL, in the top 0–30 cm of soil is also a relevant trait for selecting temporary CCs for Mediterranean woody crops. Our results show that they are also greatly affected by edaphic-climatic conditions and that they have a moderate correlation with aboveground biomass. The analysis of soil water uptake in the Córdoba plot showed no significant differences among the monospecific species and the mixtures, with ET values during the 2014/15 and 2015/16 growing seasons in the range of 325–300 mm.
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