Estimation Of Rutin Research Articles

HPTLC Quantification and Estimation of Rutin and Kaempferol using aerial parts of Ixora javanica (blume) dc. and Ixora barbata roxb. ex. sm.

Plants extracts continue to be natural sources of various drugs which play an important role in combating several diseases and infections. Flavonoids are an important class of secondary metabolites with immense potential to be used as drugs, for treating several diseases. With an aim to discover more natural sources of flavonoids to be used as drugs, leaf and flower extracts of two species of Ixora were analyzed. Current study deals with phytochemical analysis and quantification of 2 flavonoids, Rutin and Kaempferol, present in Ixora javanica and Ixora barbata using HPTLC method. The present study involves detection, quantification and comparison of both the above flavonoids present in their leaf and flower extracts. The solvents used for extraction were also compared to suggest the best solvent to be used to obtain maximum amount of flavonoids. Leaf and flower extracts of both plants were made using petroleum ether, 85% methanol and 90% ethanol solvents. HPTLC analysis indicated the presence of Rutin and Kaempferol in the extracts. The highest amount of Rutin was recorded in 85% methanolic extract of flowers of Ixora javanica (1298.6µg/ml). This was followed by 85% methanolic extract of leaf of Ixora barbata (1183.5µg/ml). The lowest amount of Rutin was observed in 90% ethanolic extract of leaf of Ixora barbata (10.9µg/ml). The highest amount of Kaempferol was observed in 90% ethanolic extract of leaf of Ixora javanica (527.8µg/ml). The lowest amount of Kaempferol was observed in 90% ethanolic extract of leaf of Ixora barbata (58.2µg/ml).

Estimation of Flavonoids in the Amranthus viridis linn A Review

Amaranthus Viridis, is traditionally used for treatment of constipation, inflammation, eczema, bronchitis, anaemia, leprosy. Flavonoids are a group of polyphenolic compounds, which are widely distributed throughout the plant kingdom. Flavonoids like Rutin and Quercetin possess many biochemical effects like inhibition of enzymes, regulatory role on different hormones and pharmacological activities like antimicrobial, antioxidant, and anticancer, antihepatotoxic, protection of cardio vascular system. An HPLC method was developed for the estimation of rutin and quercetin from methanol herbal extract of Amaranthus viridis. Flavonoids like Rutin and quercetin possess many biochemical effects like inhibition of enzymes, regulatory role on different hormones and pharmacological activities like antimicrobial, antioxidant, and anticancer, antihepatotoxic, protection of cardio vascular system.

Comparative quantitative phytochemical and HPTLC analysis of two Euphorbiaceae family plants under the name Dugdhika

Euphorbia hirta and Euphorbia thymifolia are plants with the same regional name “Dudheli” in Gujarat region, India. The present study has been conducted with comparative analysis of two plants, E. hirta and E. thymifolia, for total flavonoid content, total phenolic content, and quantification of biomarkers (rutin, gallic acid, and quercetin) by using high-performance thin-layer chromatography (HPTLC). Total phenolic contents (TPC) and total flavonoid contents (TFC) were evaluated by Folin‒Ciocalteu method and aluminum chloride colorimetric method, respectively. An improved HPTLC method was developed and validated for the simultaneous estimation of rutin, quercetin, and gallic acid. TPC was found more in the methanolic extract of E. hirta, and TFC was found almost the same in ethyl acetate extracts of both plants. Both plants showed the presence of rutin, quercetin, and gallic acid. The results of the HPTLC study provided a valuable bioactive marker content comparison between two species of the Euphorbiaceae family.

Interspecies Anticancer and Antimicrobial Activities of Genus Solanum and Estimation of Rutin by Validated UPLC-PDA Method.

Solanaceae is one of the highly diverse plant families of which Solanum is the largest genera (1700 species) containing several pharmacological properties like anticancer and antimicrobial. This motivated us to explore the anticancer (against HepG2, HEK-293, and MCF-7 cells) and antimicrobial (against S. aureus, E. coli, P. aeruginosa, and C. albicans) properties of S. schimperianum, S. villosum, S. coagulans, S. glabratum, S. incanum, and S. nigrum along with rutin estimation by UPLC-PDA method. Of the studied Solanum extracts, S. nigrum exhibited significant cytotoxic property against HepG2 (IC50: 20.4 μg/mL) and MCF-7 (IC50: 30.1 μg/mL); S. coagulans showed toxicity against HepG2 (IC50: 28.4 μg/mL) and HEK-293 cells (IC50: 25.7 μg/mL) compared to 5-Fluorouracil (standard). Compared to these, extracts of S. coagulans and S. glabratum exhibited relatively high antimicrobial potency (MIC: 0.4-1.6 mg/mL). Nonetheless, all Solanum extracts significantly reduced the biofilm against PAO1-strain. Rutin was detected in all extracts with the highest content (53.79 μg/mg) in S. coagulans that supported its strong antimicrobial and anticancer properties. Molecular docking analysis showing strong binding of rutin with human DNA and proteins (DNA Topoisomerase IIα and E. coli DNA gyrase B) supported the anticancer and antimicrobial activities of Solanum species.

Application of hplc method for simultaneous estimation of rutin and myricetin from micro and nano powders of leaves of syzygium jambos (l.) Alston

Application of hplc method for simultaneous estimation of rutin and myricetin from micro and nano powders of leaves of syzygium jambos (l.) Alston

High performance liquid chromatographic method for simultaneous estimation of rutin and myricetin from dried leaf powder of syzygiumjambos (l.) Alston.

High performance liquid chromatographic method for simultaneous estimation of rutin and myricetin from dried leaf powder of syzygiumjambos (l.) Alston.

High performance liquid chromatographic method for simultaneous estimation of rutin and myricetin from dried leaf powder of syzygiumjambos (l.) Alston.

High performance liquid chromatographic method for simultaneous estimation of rutin and myricetin from dried leaf powder of syzygiumjambos (l.) Alston.

Quantification of rutin in rat's brain by UHPLC/ESI-Q-TOF-MS/MS after intranasal administration of rutin loaded chitosan nanoparticles.

Rutin (RT), an antioxidant drug, has been utilized to treat cerebral ischemia hence a sensitive quantification method for estimation of RT in brain homogenate is necessary to develop. This study aims to prepare RT loaded Chitosan Nanoparticles (RT-CS-NPs) develop and validate ultra-high performance liquid chromatography-electrospray ionization-synapt mass spectrometric method Synapt Mass Spectrometry (Synapt MS) (UHPLC/ESI-QTOF-MS/MS) for quantification of RT in brain homogenate from Wistar rat. The process of chromatographic separation was carried out on Waters ACQUITY UPLC™ with the components of separation in detail as; column: BEH C-18 with dimension as 2.1 mm×100 mm and particle size 1.7 µm, mobile phase: acetonitrile (85 % v/v/v): 2 mM ammonium formate (15 % v/v/v): formic acid (0.1 % v/v/v) and flow rate: 0.25 mL/min. Liquid-liquid extraction method (LLE), in mixture, i.e. ethyl acetate:acetonitrile, was considered to optimize the recovery of analyte from the brain homogenate of Wistar rat. Over a total run time of 5 minutes, the elution time for RT and internal standard (IS), i.e. Tolbutamide, observed was 2.67 and 2.82 min respectively whereas the transition observed for RT and IS was at m/z 611.1023/303.1071 and 271.1263/155.1073, respectively. Results, regarding various processes and parameters studied for RT as summarized, established a linear dynamic range over a concentration range of 1.00 ng/mL - 1000.0 ng/mL with r2; 0.9991±0.0010. Accuracy for intra and inter-assay in terms of % CV revealed a range of 0.45- 2.11 whereas lower limit of detection (LOD) and quantitation (LOQ) observed was 0.09 ng/mL and 0.142 ng/mL, respectively. The analyte stability as well as method specificity and accuracy, i.e. recovery > 86 %, supports the idea for application of current developed method in order to quantify and evaluate the RT-loaded-CS-NPs for RT determination in brain homogenate after intranasal drug delivery.

Estimation of Rutin and Ascorbic Acid in Some Libyan Herbal Plants by RP-HPLC

Objective: A combination of rutin and ascorbic acid were exploited in several pharmaceutical activities and so simple and high sensitive liquid chromatographic method for the simultaneous determination of the cited ingredients in methanolic extracts of different Libyan herbal plants was proposed. Methods: The chromatographic analysis was performed using 250 × 4.6 mm Brownlee BIO C18 column, 5 μm particle size, with UV detection at 254 nm and isocratic elution of methanol–phosphate buffer pH 3.2, which is initially in 45:55 (v/v) for 0.5 min (equilibrium state), then varied to 60:40 (v/v) isocratic for 8 min and then back to 45:55 for 2 min, at a flow rate 1 ml/min and at ambient temperature. Results: The percentages of rutin and ascorbic acid found were 0.7, 0.1 in Salvia fruticosa, 1.72, 2.31 in Thymus vulgaris, 0.44, 0.35 in Rosemary officinalis, 2.87, 3.56 in Matricaria chamomilla L., 2.10, 0.62 in Artemisia absinthium respectively. Conclusion: The proposed method was suitable for the identification and quantification of the binary combination of rutin and ascorbic acid in Libyan herbal plants.

FAST ANALYSIS AND VALIDATION OF RUTIN IN ANTI-PSORIATIC AYURVEDIC FORMULATION BY HPLC

777 Oil is a topically applied Ayurvedic formulation used for the effective treatment of psoriasis. The formulation is composed of leaf extract of Wrightia tinctoria and Oleum Cocus nucifera. A selective, sensitive, and reproducible HPLC method was developed for analyzing marker compound of Wrightia tinctoria (Rutin) in 777 Oil for routine standardization purpose. The chromatography was performed on Phenomenex C18 (250 × 4.6 mm, 5.0 µm particle) column using methanol-water (60:40, v/v) as mobile phase; adjusted to pH 3.0 by orthophosphoric acid. The flow rate was 1.0 mL/min. with detection at 360 nm. The values of retention times and capacity factor were 3.88 and 0.40, respectively. The calibration plot showed a good linear relationship between response curve and concentration in the range of 1.0–1000.0 µgmL−1 with regression coefficient 0.9998. The detection (LOD) and quantification (LOQ) limits were found 27.0 and 95.0 ngmL−1 respectively. The statistical analysis proved that the method was precise, reproducible, selective, and accurate for the analysis of rutin in 777 Oil. The developed HPLC method is useful for the qualitative and quantitative estimation of rutin in 777 Oil and other products of traditional systems of medicine.

Estimation of bioflavonoids in <i>Amaranthus caudatus Linn</i>. by HPLC

Flavonoids are present in the Amaranthus caudatus Linn, Flavonoids like Rutin and quercetin possess many biochemical effects like inhibition of enzymes, regulatory role on different hormones and pharmacological activities like antimicrobial, antioxidant, anticancer, antihepatotoxic, protection of cardio vascular system. An HPLC method was developed for the estimation of rutin and quercetin from methanol herbal extract of Amaranthus caudatus.

High-performance thin-layer chromatographic method for estimation of rutin in medicinal plants

A sensitive high-performance thin-layer chromatographic (HPTLC) method has been developed for estimation of rutin. The method was validated for precision (intra- and inter-day), repeatability, and accuracy and then adopted for estimation of the rutin content of the leaves of Tephrosia purpurea, Leptadenia reticulata, and Ruta graveolens. The method was found to be accurate, precise, and suitable for estimation of rutin in medicinal plants.

Quantitative Survey of Rutin-containing Plants Part 1

AbstractQuantitative estimation of rutin in fresh leafy shoots, fruits and fruit peels of thirty four plants of Pakistan was carried out. The highest amounts were found in seeds of Sophora secundiflora (8.4%) and leaves of Mangifera indica (5.2%).