PURPOSE: To determine the agreement between dual-energy x-ray absorptiometry (DXA) and multi-frequency bioelectrical impedance analysis (BIA) for estimating body fat percentage (BF%), fat mass (FM), and fat-free mass (FFM) in a Hispanic population. METHODS: One-hundred eighty-one individuals (males: n = 84; females: n = 97) of Hispanic descent had body composition estimated via DXA and BIA. Participants completed all testing wearing compression gear and provided a urine sample to assess hydration status. Adequate hydration was considered at a urine specific gravity value <1.029. Agreement between DXA and MF-BIA BF%, FM, and FFM was assessed using Pearson correlations, linear regression, and Bland-Altman analyses. Analyses yielded the standard error of the estimate (SEE), constant error (CE), 95% limits of agreement (LOA), and proportional bias for the entire group and within sexes. RESULTS: For BF%, BIA displayed similar CE ± 95% LOA for the sample (-3.17 ± 5.45%), males (-3.2 ± 5.5%), and females (-3.2 ± 5.4%) compared to DXA. Correlation analyses indicated near-perfect associations (sample: r = 0.96, males: r = 0.93, and females: r = 0.93); however, a moderate proportional bias was present for females (r = 0.48). The sample (r = 0.22) and males (r = -0.04) had trivial-to-no proportional bias. Regarding FM, BIA exhibited CE ± 95% LOA values of -1.4 ± 4.2 kg for the sample, -1.9 ± 4.6 kg for males, and -0.9 ± 3.6 kg for females. All groups displayed near-perfect associations (sample: r = 0.99, males: r = 0.97, and females: r = 0.99), despite a strong proportional bias for females (r = 0.68) and moderate bias for the sample (r = 0.36). No proportional bias was observed for males (r = -0.02). For FFM, males demonstrated the largest CE ± 95% LOA (1.6 ± 4.6 kg), compared to the sample (1.2 ± 3.9 kg) and females (0.9 ± 3.4 kg) when BIA was compared to DXA. Near-perfect associations were observed for all groups (sample: r = 0.98; males: r = 0.97; females: r = 0.97). No proportional biases existed for the sample (r = -0.01) or males (r = -0.10); however, females exhibited a moderate, negative bias (r = -0.38). CONCLUSIONS: Due to the observed moderate-to-strong proportional biases within body composition estimates, particularly for Hispanic females, the need for ethnic-specific algorithms is warranted.