Fractional vegetation cover (FVC) is an essential metric for valuating ecosystem health and soil erosion. Traditional ground-measuring methods are inadequate for large-scale FVC monitoring, while remote sensing-based estimation approaches face issues such as spatial scale discrepancies between ground truth data and image pixels, as well as limited sample representativeness. This study proposes a method for FVC estimation integrating uncrewed aerial vehicle (UAV) and satellite imagery using machine learning (ML) models. First, we assess the vegetation extraction performance of three classification methods (OBIA-RF, threshold, and K-means) under UAV imagery. The optimal method is then selected for binary classification and aggregated to generate high-accuracy FVC reference data matching the spatial resolutions of different satellite images. Subsequently, we construct FVC estimation models using four ML algorithms (KNN, MLP, RF, and XGBoost) and utilize the SHapley Additive exPlanation (SHAP) method to assess the impact of spectral features and vegetation indices (VIs) on model predictions. Finally, the best model is used to map FVC in the study region. Our results indicate that the OBIA-RF method effectively extract vegetation information from UAV images, achieving an average precision and recall of 0.906 and 0.929, respectively. This method effectively generates high-accuracy FVC reference data. With the improvement in the spatial resolution of satellite images, the variability of FVC data decreases and spatial continuity increases. The RF model outperforms others in FVC estimation at 10 m and 20 m resolutions, with R2 values of 0.827 and 0.929, respectively. Conversely, the XGBoost model achieves the highest accuracy at a 30 m resolution, with an R2 of 0.847. This study also found that FVC was significantly related to a number of satellite image VIs (including red edge and near-infrared bands), and this correlation was enhanced in coarser resolution images. The method proposed in this study effectively addresses the shortcomings of conventional FVC estimation methods, improves the accuracy of FVC monitoring in soil erosion areas, and serves as a reference for large-scale ecological environment monitoring using UAV technology.