We measured concentrations of sodium and creatinine in previously validated overnight urine and 24-h urine of 79 healthy infants of 3 to 5 y of age in two cities in Miyagi Prefecture, Japan. Thereafter, we estimated daily salt intake from daily urinary sodium excretion and daily urinary volume. Corrections for potential sweat loss of sodium chloride were conducted based on urinary volume and urinary creatinine concentration. We also measured urinary volume and urinary sodium concentration in infants who failed to provide complete urine samples. The estimated salt intake did not differ between boys and girls, but varied between the two cities. The estimated salt intake per unit of body weight differed neither between genders nor between cities. The grand means for estimated salt intake and that per unit of body weight were 5.5 g/d (SD 1.8) and 0.32 g/kg/d (SD 0.10), which supported previous results both in Japan and abroad. It was also possible to roughly estimate salt intake from sodium concentration in overnight urine or incomplete 24-h urine, and published urine volume.