The study addressed the limitations of classical statistical methods when dealing with ambiguous data, emphasizing the importance of adopting neutrosophic statistics as a more effective alternative. Classical methods falter in managing uncertainty inherent in such data, necessitating a shift towards methodologies like neutrosophic statistics. To address this gap, the research introduced a novel sampling approach called “neutrosophic median ranked set sampling” and incorporated neutrosophic estimators tailored for estimating the population mean in the presence of ambiguity. This modification aims to address the inherent challenges associated with estimating the population mean when dealing with neutrosophic data. The methods employed involved modifying traditional ranked set sampling techniques to accommodate neutrosophic data characteristics. Additionally, neutrosophic estimators were developed to leverage auxiliary information within the framework of median-ranked set sampling, enhancing the accuracy of population mean estimation under uncertain conditions. The methods employed involved modifying traditional ranked set sampling techniques to accommodate neutrosophic data characteristics. Bias and mean squared error equations for the suggested estimators were provided, offering insights into their theoretical underpinnings. To illustrate the effectiveness and practical applications of the proposed methodology and estimators, a numerical demonstration and simulation study have been conducted using the R programming language. The key results highlighted the superior performance of the proposed estimators compared to existing alternatives, as demonstrated through comprehensive evaluations based on mean squared error and percentage relative efficiency criteria. The conclusions drawn underscored the effectiveness of the neutrosophic median ranked set sampling approach and suggested estimators in estimating the population mean under conditions of uncertainty, particularly when utilizing neutrosophic auxiliary information and validated real-life applicability. The methodology and estimators presented in the study were shown to yield interval-based results, providing a more realistic representation of uncertainty associated with population parameters. This interval estimation, coupled with minimum mean squared error considerations, enhanced the efficacy of the estimators in determining population mean values. The novelty of the work lies in its introduction of a tailored sampling approach and estimators designed specifically for neutrosophic data, filling a significant gap in the literature. By extending classical statistics to accommodate ambiguity, the study offers a substantial advancement in statistical methodology, particularly in domains where precise data is scarce and uncertainty is prevalent. Furthermore, the empirical validation through numerical demonstrations and simulation studies using the R programming language adds robustness to the proposed methodology and contributes to its practical applicability.