An experiment was conducted to determine the genetic distance and identity at insulin-like growth factor 1 (IGF-1) gene locus in some indigenous cattle breeds in Nigeria. One (1) Local Government Area (LGA) each from Adamawa, Taraba, Gombe and Sokoto States was purposively selected which include Mubi North, Yorro, Balanga, and Wurno Local Government Areas, respectively. The snowball method was used to sample (96) cattle consisting of (24) Adamawa Gudali, (24) Sokoto Gudali, (24) Bunaji and (24) Rahaji cattle from pastoralists in many communities of the LGAs. Blood samples were collected from the animals through jugular venepuncture. DNA extraction was done using Zymo Quick DNA™ Mini prep kit. NanoDrop 1000 spectrophotometer was used for the determination of DNA quality and quantity using DNA purification kit. Genetic distances among the breeds of cattle were calculated using distance measures with the aid of Gen AIEx software package. The highest level of genetic distance estimate was observed between Rahaji and Bunaji cattle breeds (0.085) while the closest genetic distance (0.001) was observed between Sokoto Gudali and Bunaji. The highest genetic identities were recorded between Sokoto Gudali and Bunaji (0.999) cattle breeds whereas the closest matrix of genetic identity (0.904) was recorded between Rahaji and Sokoto Gudali cattle. Cluster analysis using UPMGA revealed that Bunaji and Sokoto Gudali cattle formed the first cluster which is separate from Adamawa, which formed the second cluster. Rahaji cattle formed the third cluster, which is far and distant from the first and second clusters at IGF-1 gene locus. Rahaji cattle had the longest genetic distance with other three breeds of cattle. High genetic distance and identity existed among some indigenous breeds of cattle studied.
Read full abstract