Ester Components Research Articles

Isovaleryl Sucrose Esters from Atractylodes japonica and Their Cytotoxic Activity.

Cancer represents one of the most significant health challenges currently facing humanity, and plant-derived antitumour drugs represent a prominent class of anticancer medications in clinical practice. Isovaleryl sucrose esters, which are natural constituents, have been identified as having potential antitumour effects. However, the mechanism of action remains unclear. In this study, 12 isovaleryl sucrose ester components, including five new (1-5) and seven known compounds (6-12), were isolated from the roots of Atractylodes japonica. The structures of the compounds were elucidated using 1D and 2D-NMR spectroscopy, complemented by HR-ESI-MS mass spectrometry. The cytotoxic activities of all the compounds against human colon cancer cells (HCT-116) and human lung adenocarcinoma cells (A549) were also evaluated using the CCK8 assay. The results demonstrated that compounds 2, 4, and 6 were moderately inhibitory to HCT-116 cells, with IC50 values of 7.49 ± 0.48, 9.03 ± 0.21, and 13.49 ± 1.45 μM, respectively. Compounds 1 and 6 were moderately inhibitory to A549, with IC50 values of 8.36 ± 0.77 and 7.10 ± 0.52 μM, respectively. Molecular docking revealed that compounds 1-9 exhibited a stronger affinity for FGFR3 and BRAF, with binding energies below -7 kcal/mol. Compound 2 exhibited the lowest binding energy of -10.63 kcal/mol to FGFR3. We screened the compounds with lower binding energies, and the protein-ligand complexes already obtained after molecular docking were subjected to exhaustive molecular dynamics simulation experiments, which simulated the dynamic behaviour of the molecules in close proximity to the actual biological environment, thus providing a deeper understanding of their functions and interaction mechanisms. The present study provides a reference for the development and use of iso-valeryl sucrose esters in the antitumour field.

Mechanism and technological method of leaching metals from waste lithium-ion batteries by synergistic action of natural organic reductants and mechanochemical method

In this study, litchi peel powder (LPP) was selected as a natural organic reductant to study the mechanism of green leaching of valuable metals from spent lithium-ion batteries (LIBs) by ball milling. LPP and cathode material were co-ball-milled(CBM) with water and then leached, LPP-assisted ball milling reduces the agglomeration tendency of the particles and increases the dispersion. Premixing by CBM makes the LPP and the cathode material fully contracted and tightly fused, and the mechanical force provides the activation energy of the reaction to accelerate the leaching. The leaching rates of Li, Co, Mn and Ni were 99.21 wt%, 96.52 wt%, 98.21 wt% and 94.79 wt% when LPP was added at 0.15 g and only 0.2 mol/L H3Cit was used. Compared with direct leaching, the amount of organic acids was reduced by 86.67 %. The proanthocyanidins contained in the LPP are the key to act as a reducing agent, and proanthocyanidins are the products of the combination of anthocyanins and glycosides. The natural phenolic and ester components in LPP are hydrolyzed into phenolic acids (protocatechulic acid, salicylic acid) and flavonoids (catechins) under the action of H3Cit, which accelerates the dissolution of metal oxides. The mechanism of natural organic reductant in auxiliary ball milling and organic acid leaching was clarified, which provided a new idea for the selection of ball milling auxiliary materials and proposed a economic and environmental protection method to leach valuable metals from spent LIBs.

Effects of co‐fermentation of high‐yield ester Zygosaccharomyces rouxii and Saccharomyces cerevisiae on the quality of rice wine

AbstractBackgroundAt present, rice wine has the characteristics of fast fermentation, energy saving, and simple production due to the addition of commercial Saccharomyces cerevisiae. However, this kind of fermented rice wine has the disadvantages of single flavor and weak taste. To address this concern, the combination of Saccharomyces cerevisiae and non‐Saccharomyces yeast has been identified as an effective approach to enhance the quality of fermented rice wine. This research aims to further enhance the ethyl ester and higher alcohol components of rice wine by utilizing the co‐fermentation method of high‐yield ester Zygosaccharomyces rouxii and Saccharomyces cerevisiae, thereby increasing the aroma complexity of rice wine.ResultsAccording to the fermentation performance and gas chromatography–mass spectrometry (GC–MS) analysis, it was found that S. cerevisiae GJM and Z. rouxii SC01 have a strong metabolic ability for glucose and sucrose. Furthermore, the crucial alcohols such as isobutanol, isoamyl alcohol, and phenylethyl alcohol increased by 85%, 29%, and 138%, respectively, when compared to single S. cerevisiae GJM. Additionally, the ethyl acetate and phenylethylene acetate were increased by 1.35 and 1.45 times, respectively, when compared to single S. cerevisiae GJM. These findings were comparable with the results of sensory evaluation and electronic nose analysis, where the fruit flavor score and the content of mellow substances were found to be higher.ConclusionThese findings suggest that the co‐fermentation of S. cerevisiae and high‐yield ester Z. rouxii has the potential to enhance the quality and diversity of aroma in liquid rice wine.

Synthesis of Biodiesel from Refined Waste Cooking Oil with Active Natural Zeolite

This research evaluates refining waste cooking oil with active natural zeolite, utilizes it for biodiesel synthesis, and characterizes the synthesized methyl ester. This experimental laboratory research was carried out: (1) activation of natural zeolite, (2) refining waste cooking oil with active natural zeolite, (3) determination of free fatty acid (FFA) content of the oil, (4) transesterification with KOH catalyst, (5) characterization of the synthesized methyl esters according to the SNI 7182:2015, and (6) identification of the methyl ester component by GC-MS. The results showed that the FFA content of the refined waste cooking oil using active natural zeolite with concentrations of 10%, 20%, and 30% per mass of oil were 2.97, 2.0, and 1.56%, respectively. The yield of methyl ester transesterified with various concentrations of KOH (0.8, 1.0, and 2% per mass of oil), respectively, were 80.13, 85.34, and 74.98%. The synthesized methyl ester resulted in the characteristics: 0.867 g/mL of density; 5.72 cSt of viscosity; 1.449 of refractive index; 1.46 g KOH/g of acid number; and 0.023% of water content. Identification of GC-MS obtained the synthesized methyl esters, which include: 46.10% of methyl hexadecanoic, 41.68% of methyl 9-octadecanoic, 6.42% of methyl octadecanoic, and 5.80% of methyl eicosanoic.

Development of the cholestosome, a novel delivery system made exclusively from cholesteryl esters

AbstractA novel vesicular drug delivery system has been created using only cholesteryl esters. The uncharged nature of the esters requires a formulation process that uses energy, in the form of sonication, to facilitate the cholesteryl esters formation into vesicles. The nature of the material choices renders the vesicles resistant to pH degradation between a pH of 2 and 10. Storage condition at 4°C for 9 months shows a stability of encapsulated materials as well as vesicles. The formulation process is a modified reverse phase method in which the esters are dissolved in an organic solvent, which is then removed and the aqueous component is then added with sonication in a temperature‐controlled water bath. A wide variety of materials can be encapsulated in these vesicles, which are called cholestosomes. Delivery of the cholestosome‐encapsulated materials is a function of the ester component structure and not the encapsulated molecule. Once encapsulated, the vesicles deliver the materials into cells. The potential for oral availability was explored using chylomicrons. Successful delivery of the cholestosome encapsulated into chylomicrons has enhanced the effectiveness of delivery to cells. Analytical methods have been developed to analyze the materials chosen from the cholesteryl esters as well as the encapsulated materials.Practical Applications: The creation of the cholestosome vesicle opens up completely new areas for encapsulating and delivering materials. The cholesteryl ester structure that makes up the vesicles is impervious to pH and bile salts, making cholestosomes ideal for oral delivery of drugs, such as oral insulin. Intracellular delivery of cholestosome vesicles enables multiple applications, from killing latent infections to preventing viral replication (irrespective of the virus) and delivering nucleic acid therapeutics to cure genetic disease. These vesicles pass the blood–brain barrier and could be used for treatments of Alzheimer's and related neurodegenerative disease. Cholestosomes can also be used in cosmetic formulations as the vesicles pass the dermis. They even could be used in food applications as the materials are considered generally regarded as safe.

Evaluation of Physicochemical Characteristics and Sensory Properties of Cold Brew Coffees Prepared Using Ultrahigh Pressure under Different Extraction Conditions.

Although cold brew coffee is becoming increasingly popular among consumers, the long coffee extraction time is not conducive to the further development of the market. This study explored the feasibility of ultrahigh pressure (UHP) to shorten the time required for preparing cold brew coffee. The effects of pressure and holding time on the physicochemical characteristics and sensory evaluation of UHP-assisted cold brew coffee were also determined. The extraction yield; total dissolved solid, total phenol, and melanoid content; antioxidant capacity; and trigonelline and chlorogenic acid contents of UHP-assisted cold brew coffee increased as the pressure increased. The extraction yield and the total dissolved solid, total phenol, total sugar, and chlorogenic acid and trigonelline contents were higher when the holding time was longer. The HS-SPME-GC/MS analysis demonstrated that the furan, aldehyde, and pyrazine contents in coffee increased as the pressure and holding time increased. The pressure did not significantly impact the concentrations of volatile components of esters and ketones in coffee samples. However, the increase in holding time significantly increased the ester and ketone contents. The sensory evaluation results revealed that as pressure rose, the intensities of nutty, fruity, floral, caramel, and sourness flavors increased, whereas bitterness and sweetness decreased. Longer holding time increased nutty, caramel, sour, bitter, sweet, and aftertaste flavors. Principal component analysis (PCA) results indicated that holding time is a more crucial factor affecting the physiochemical indices and flavor characteristics of coffee. UHP can shorten the preparation time of cold brew coffee. Pressure and holding time significantly affected the physiochemical indices and volatile components of UHP-assisted cold brew coffee. UHP-assisted cold brew coffee had lower bitterness, higher sweetness, and a softer taste than conventional cold brew coffee.

Structural and Rheological Properties of Exopolysaccharides Produced by Some Lactic Acid Bacterial Strains Isolated from Palm Wine

Aims: This study evaluated the physical, chemical and rheological properties of exopolysaccharides (EPSs) produced by Lactic Acid Bacteria (LAB) isolated from palm wine.
 Materials and Methods: EPSs from palm wine LAB strains were produced on 6% sucrose broth, purified and freeze-dried prior to analyses. Molecular weights (MW), rheological and structural composition (functional groups) of the EPSs were determined using standard methods and Fourier transform infrared spectroscopy (FTIR).
 Results: The average MW of the EPSs ranged from 2.02×106 to 6.53×106 Da while the flow index (n) values ranged from 0.03-3.13 at 0.2%, 0.06-1.51 at 0.4%, 0.38 - 1.85 at 0.6%, 0.14 - 2.26 at 0.8% and 0.55 - 6.42 at 1% concentrations at elevated temperatures for EPS solutions from the ten LAB species. The FTIR spectrum revealed prominent peaks of various groups of OH (3420 cm-1) and CH3 bending (2090 cm-1) in all the EPSs corresponding to both hydroxyl and amine groups, and aliphatic C-H bonds, respectively. EPS synthesized by Leuconostoc lactis, Lactococcus lactis subsp lactis and Lactiplantibacillus plantarum showed weak absorption peaks (1148 – 1145 cm-1) indicating the C-O-C and C-O bonds, while absorption peaks of Lactobacillus lactis, Lactobacillus acidophilus and Lactiplantibacillus plantarum (1267 – 1253 cm-1) indicated O- acetyl ester and other non-sugar components.
 Conclusions: The FTIR spectra, rheological properties and molecular weight of EPSs synthesized by the ten LAB strains indicated potentials that could be exploited in different industrial applications, and as stabilizers in food industries

Symbiosis preservation: Putative regulation of fatty acyl-CoA reductase by miR-31a within the symbiont harboring bacteriome through tsetse evolution.

Tsetse flies are the sole vectors of African trypanosomes. In addition to trypanosomes, tsetse harbor obligate Wigglesworthia glossinidia bacteria that are essential to tsetse biology. The absence of Wigglesworthia results in fly sterility, thus offering promise for population control strategies. Here, microRNA (miRNAs) and mRNA expression are characterized and compared between the exclusive Wigglesworthia-containing bacteriome and adjacent aposymbiotic tissue in females of two evolutionarily distant tsetse species (Glossina brevipalpis and G. morsitans). A total of 193 miRNAs were expressed in either species, with 188 of these expressed in both species, 166 of these were novel to Glossinidae, and 41 miRNAs exhibited comparable expression levels between species. Within bacteriomes, 83 homologous mRNAs demonstrated differential expression between G. morsitans aposymbiotic and bacteriome tissues, with 21 of these having conserved interspecific expression. A large proportion of these differentially expressed genes are involved in amino acid metabolism and transport, symbolizing the essential nutritional role of the symbiosis. Further bioinformatic analyses identified a sole conserved miRNA::mRNA interaction (miR-31a::fatty acyl-CoA reductase) within bacteriomes likely catalyzing the reduction of fatty acids to alcohols which comprise components of esters and lipids involved in structural maintenance. The Glossina fatty acyl-CoA reductase gene family is characterized here through phylogenetic analyses to further understand its evolutionary diversification and the functional roles of members. Further research to characterize the nature of the miR-31a::fatty acyl-CoA reductase interaction may find novel contributions to the symbiosis to be exploited for vector control.

Phytochemical analysis and insecticidal activities of seed extracts from Oenanthe pimpinelloides L. treated paper samples vs. Tribolium castaneum

The utilization of plant extraction products from Oeneanthe pimpinelloides (Apiaceae family) seeds were investigated in terms of their use as an insecticide control of packaging materials. The aim was to investigate their insecticidal effects against the flour beetle Tribolium castaneum. The Oeneanthe pimpinelloides seeds were extracted with methanol. By using the liquid-liquid extraction method, the hexane extract (II) was separated from the methanol extract (I) and hexane and methanol were evaporated. Then, the chemical composition of each sample was determined via gas chromatography–mass spectrometry. The methanol extract predominantly contained tetrahydrofuran, 1-methoxy-2-propanol, 1-methoxy, 2-butoxyethanol, 1-phenylethanone, cyclohexene carboxylate derivative, (3-phenyl-2-propynylidene) cyclopropane, diphenyldiazene, and dihydroxypropyl ester components, while the hexane fraction contained nonane, 1-octanol, decane, undecane, tridecane, alkyl benzene, benzene sulfonic acid, benzoxazine, and hexadecanoic acid components, as well as some derivatives of them. Each fraction was dissolved in DMSO for impregnation on filter paper. The insecticide effects of the paper samples were determined against Tribolium castneum. According to the results, the mortality started after 3 d for each fraction. After 4 d, the hexane fraction indicated total mortality in comparison with the methanol fraction, which showed partial mortality (3/5).

On The Antioxidant Activity and Cytotoxicity of Echinops spinosus and Fagonia mollis in Wadi Hagul, Egypt

Echinops spinosus and Fagonia mollis were two plants collected from Wadi Hagul, Eastern desert, Egypt during spring and autumn season. Secondary metabolites were at their highest levels in both tested plants during autumn season, and the antioxidant capabilities of these secondary metabolites were physiologically relevant to plant stress response systems. The invitro studies revealed that both plants have antioxidant properties and a promising cytotoxic activity against a breast cancer cell line (MCF-7). Based on the least of IC50 value of this extract and fractions, GC/ MS analysis was done for E. spinosus and F. mollis methylene chloride fractions and F. mollis crude extract. The GC/MS analysis disclosed that both E. spinosus and F. mollis methylene chloride fractions and their crude extracts, have predominant phytochemical compounds that have cytotoxic activity. The presence of pentanoic acid, 4-oxo-, butyl ester in E. spinosus methylene chloride fraction, and mome inositol and 9,12,15-octadecatrienoic acid, methyl ester components in F. mollis crude extract as well as phytol and (-)-loliolide in its methylene chloride fraction.

The Functional Characterization of Carboxylesterases Involved in the Degradation of Volatile Esters Produced in Strawberry Fruits.

Volatile ester compounds are important contributors to the flavor of strawberry, which affect consumer preference. Here, the GC-MS results showed that volatile esters are the basic aroma components of strawberry, banana, apple, pear, and peach, and the volatile esters were significantly accumulated with the maturation of strawberry fruits. The main purpose of this study is to discuss the relationship between carboxylesterases (CXEs) and the accumulation of volatile ester components in strawberries. FaCXE2 and FaCXE3 were found to have the activity of hydrolyzing hexyl acetate, Z-3-hexenyl acetate, and E-2-hexenyl acetate to the corresponding alcohols. The enzyme kinetics results showed that FaCXE3 had the higher affinity for hexyl acetate, E-2-hexenyl acetate, and Z-3-hexenyl acetate compared with FaCXE2. The volatile esters were mainly accumulated at the maturity stages in strawberry fruits, less at the early stages, and the least during the following maturation stages. The expression of FaCXE2 gradually increased with fruit ripening and the expression level of FaCXE3 showed a decreasing trend, which suggested the complexity of the true function of CXEs. The transient expression of FaCXE2 and FaCXE3 genes in strawberry fruits resulted in a significantly decreased content of volatile esters, such as Z-3-hexenyl acetate, methyl hexanoate, methyl butyrate, and other volatile esters. Taken together, FaCXE2 and FaCXE3 are indeed involved in the regulation of the synthesis and degradation of strawberry volatile esters.

An experimental assessment of using waste plastic fuel as an additive in a DI diesel engine running on Jatropha biodiesel blends

The sole objective of the current work is to assess the effects of adding waste plastic fuel to diesel and jatropha biodiesel. In order to test this, seven distinct fuel samples made up of jatropha biodiesel-diesel, WPF-jatropha biodiesel-diesel, and regular diesel as a reference fuel were created using predefined volumetric proportions. After thorough characterization, studies are carried out employing spectroscopic methods such as FTIR, elemental analysis, and GC-MS. Finally, a direct-injection single-cylinder diesel engine underwent performance and emission tests. It has been discovered that adding WPF to biodiesel lowers its density, flash point, and viscosity. When WPF is added to biodiesel blends, the biodiesel’s cold flow properties are improved, and its calorific value and diesel index are raised. According to FTIR analysis of the fuel samples, the WPF mixed biodiesel, biodiesel, and diesel all share certain common functional groups. According to GC-MS examination of the fuel samples, all of the blends contain some aliphatic and aromatic chemicals as well as diglycerides, triglycerides, and ester components. WPF blended fuels exhibit improved thermal efficiency under partial loading situations, according to engine performance tests. It is discovered that the WPF blended fuel has a lower exhaust temperature than biodiesel. Compared to biodiesel and diesel, WPF mixed fuel has higher HC emissions but lower NOx emissions. Thus, it is advantageous to mix waste plastic fuel with biodiesel because doing so lowers NOx emissions while simultaneously raising the calorific value and diesel index of the biodiesel.

Mixed microalgae-food waste cake for feeding of Hermetia illucens larvae in characterizing the produced biodiesel

The industrial use of insects, their products, interactions, or activities has been known as industrial entomology. The current study focused on evaluating the effect of mixing the organic food waste with its half percentage of microalgae on the biodiesel production and characterization. The black soldier fly larvae (BSFL) were reared on the organic waste (OW) (fruits, vegetables, and kitchen) only and OW mixed with microalgae, Chlorella vulgaris. Additionally, the levels of oxidants and antioxidants in the biodiesel, after the antioxidants incorporation (ascorbic acid, α-tocopherol, and catechin), were evaluated. The BSFLBD was produced from lipid extraction of BSFL biomass by chloroform/methanol mixture, followed by acid catalyzed esterification and alkaline catalyzed transesterification. The biodiesel yield from the larvae fed on mixed OW-microalgae for 14 days was enhanced by 1.4 folds than the yield of OW group. The unsaturated oleic acid has the most dominant pattern in the fatty acid profile of BSFBD, even in different cases. Lauric (12:0) and myristic (14:0) acids constitute the majority of the methyl ester component of the biodiesel, with amounts of 25.6 and 7.1 for larvae fed on OW, and 25.9 and 7.7 for those fed on OW-microalgae. Following the antioxidants addition, the oxidant's concentration decreased in the biodiesel especially in the insects fed on mixed OW-microalgae. The microalgae added to the organic food waste enhanced the biodiesel quality and quantity of BSFL biomass. Also, the introduction of antioxidants didn't only improve the efficiency of the biodiesel, but also helped in the management of the stress parameters.

Impacts of Gestational FireMaster 550 Exposure on the Neonatal Cortex Are Sex Specific and Largely Attributable to the Organophosphate Esters

Introduction: Flame retardants (FRs) are common bodily and environmental pollutants, creating concern about their potential toxicity. We and others have found that the commercial mixture FireMaster® 550 (FM 550) or its individual brominated (BFR) and organophosphate ester (OPFR) components are potential developmental neurotoxicants. Using Wistar rats, we previously reported that developmental exposure to FM 550 or its component classes produced sex- and compound-specific effects on adult socioemotional behaviors. The underlying mechanisms driving the behavioral phenotypes are unknown. Methods: To further mechanistic understanding, here we conducted transcriptomics in parallel with a novel lipidomics approach using cortical tissues from newborn siblings of the rats in the published behavioral study. Inclusion of lipid composition is significant because it is rarely examined in developmental neurotoxicity studies. Pups were gestationally exposed via oral dosing to the dam to FM 550 or the BFR or OPFR components at environmentally relevant doses. Results: The neonatal cortex was highly sexually dimorphic in lipid and transcriptome composition, and males were more significantly impacted by FR exposure. Multiple adverse modes of action for the BFRs and OPFRs on neurodevelopment were identified, with the OPFRs being more disruptive than the BFRs via multiple mechanisms including dysregulation of mitochondrial function and disruption of cholinergic and glutamatergic systems. Disrupted mitochondrial function by environmental factors has been linked to a higher risk of autism spectrum disorders and neurodegenerative disorders. Impacted lipid classes included ceramides, sphingomyelins, and triacylglycerides. Robust ceramide upregulation in the OPFR females could suggest a heightened risk of brain metabolic disease. Conclusions: This study reveals multiple mechanisms by which the components of a common FR mixture are developmentally neurotoxic and that the OPFRs may be the compounds of greatest concern.

Predicting Speed of Sound in Fatty Acid Alkyl Esters and Biodiesels at High Pressure

Knowing the thermophysical properties of biodiesels, in particular the speed of sound, over an extended pressure range is essential to optimize injection and combustion processes in diesel engines. The aim of the present work is therefore to propose a predictive method for estimating the speed of sound in biodiesel based on the fatty acid alkyl ester profile. For that purpose, a group contribution method was first developed to predict speed of sound in fatty acid alkyl ester compounds in target p, T ranges (293–423 K and 0.1–150 MPa). Then, using experimental data from the literature supplemented by group contribution predictions, a correlation was established to relate the speed of sound in fatty acid methyl and ethyl ester components to pressure and temperature. Finally, a combination rule was used to extend the pure fatty acid alkyl ester correlations to biodiesel fuels.

Floral concretes from two tuberose cultivars for potent uses in herbal skin-care products

Flowers of tuberose (Polianthes tuberosa L.) are usually fragrant and are cultivated commercially to meet the demand of both loose and cut flower applications. This in turn generates huge floral wastes after their utilization in decoration and rituals. In this work we aim to explore waste flowers to isolate floral concrete as by-product for possible utility in skincare products. Such attempt of converting floral waste into commodity product remained apparently unexplored with cultivated flowers including tuberose. Further, no information is apparently available on the phytoconstituent of the tuberose floral concrete that constitute its waxy nature. Here we extracted floral concrete from waste flowers of two popular tuberose cultivars namely, Calcutta Single (CS) and Calcutta Double (CD) using n-hexane as solvent. Floral concrete extracts after derivatization were subjected to GC-MS analysis which revealed the presence of several fatty acid and fatty acid ester compounds. Presence of higher content of fatty acids in floral concrete of CD was detected. In contrast, ester components were found to be higher in CS, suggestive of its fragrant nature. Extracted floral concretes from both the cultivars exhibited comparable antioxidant capacities and DNA protection abilities. Further, floral concrete from CS cultivar showed higher inhibition of tyrosinase activity than CD, while elastase and hyaluronidase activities were greatly inhibited by the extracted concrete from CD cultivar suggesting their possible utility in skin-care product.

Residual palm kernel expeller as the support material and alimentation provider in enhancing attached microalgal growth for quality biodiesel production

Albeit the biodiesel production from suspended microalgal system has gained immense interests in recent years, the domineering limitation of being economically infeasible has hindered this technology from partaking into a large-scale operation. To curtail this issue, attached growth system had been introduced by various studies; however, those were still unable to alleviate the socio-economic challenges faced in commercializing the microalgal biomass production. Thus, this study had developed a novel approach in cultivating-cum-harvesting attached Chlorella vulgaris sp. microalgae, whilst using solid organic waste of palm kernel expeller (PKE) as the supporting and alimentation material for microalgal biofilm formation. The effects of three variables, namely, PKE dosage, light intensity, and photoperiod, were initially modelled and later optimized using Response Surface Methodology tool. The derived statistical models could predict the growth performances of attached microalgal biomass and lipid productivity. The optimum growing condition was attained at PKE dosage of 5.67 g/L, light intensity of 197 μmol/m2 s and photoperiod of 8 light and 16 dark hours/cycle, achieving the microalgal density and lipid content of 9.87 ± 0.05 g/g and 3.39 ± 0.28 g/g, respectively, with lipid productivity of 29.6 mg/L day. This optimum condition had led to the intensification of biodiesel quality with a high percentage of monounsaturated fatty acid, i.e., oleic acid (C18:1), encompassing 81.86% of total fatty acid methyl ester components. Given that the positive acquisition of PKE as an excellent supporting material in enhancing the microalgal density and lipid productivity that had resulted in the commercially viable biodiesel quality, this study served as a novel revolution in augmenting the microalgae and solid waste utilities for sustainable energy generation.

Effect of Cold Stabilization Duration on Organic Acids and Aroma Compounds during Vitis vinifera L. cv. Riesling Wine Bottle Storage.

During the storage of wines in bottles, especially white wines, tartrate crystallization often occurs, which reduces the commercial value of the wines and therefore needs to be avoided by performing cold stabilization treatments before bottling. However, whether different cold treatment durations impact the quality of a wine’s aroma has not yet been of special concern. This research was conducted at an industrial scale to explore how cold treatments at −5.3 °C for 10 to 15 days impact the organic acids, aroma compounds, and sensory quality of Riesling dry white wines, and the variation was documented at the end of treatment, and at 6 and 12 months of bottle storage. The results showed that cold treatments significantly reduced tartaric acid concentrations and significantly affected the concentrations of most aroma components in the wines only after 12 months of bottle storage, including the main components of esters, norisoprenoids, terpenoids, and furfural. Moreover, the concentrations of some components showed an increasing trend with the bottle storage, especially 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN), the characteristic volatile of Riesling wine, suggesting that an acidic condition resulting from cold treatment might facilitate the conversion of some aroma precursors into volatiles. In conclusion, cold stabilization treatments, within limits, can improve tartaric acid stability and could promote the conservation of aroma compounds during bottle storage without adversely affecting the aroma profile of the wines.

Evaluation of the Impact of Esterases and Lipases from the Circulatory System against Substrates of Different Lipophilicity.

Esterases and lipases can process amphiphilic esters used as drugs and prodrugs and impact their pharmacokinetics and biodistribution. These hydrolases can also process ester components of drug delivery systems (DDSs), thus triggering DDSs destabilization with premature cargo release. In this study we tested and optimized assays that allowed us to quantify and compare individual esterase contributions to the degradation of substrates of increased lipophilicity and to establish limitations in terms of substrates that can be processed by a specific esterase/lipase. We have studied the impact of carbonic anhydrase; phospholipases A1, A2, C and D; lipoprotein lipase; and standard lipase on the hydrolysis of 4-nitrophenyl acetate, 4-nitrophenyl palmitate, DGGR and POPC liposomes, drawing structure–property relationships. We found that the enzymatic activity of these proteins was highly dependent on the lipophilicity of the substrate used to assess them, as expected. The activity observed for classical esterases was diminished when lipophilicity of the substrate increased, while activity observed for lipases generally increased, following the interfacial activation model, and was highly dependent on the type of lipase and its structure. The assays developed allowed us to determine the most sensitive methods for quantifying enzymatic activity against substrates of particular types and lipophilicity.

Synthesis of methyl esters from palm oil, candlenut oil, and sunflower seed oil and their corrosion phenomena on iron nail

<abstract> <p>Biodiesel products show corrosive properties. Biodiesel contains components of saturated and unsaturated esters which tend to be unstable, sensitive to light, temperature, and metal ions. Thus, the study aims to synthesize biodiesel from various vegetable oils (palm oil, sunflower seed oil, and candlenut oil), and to analyze its corrosiveness to ferrous nails and characterization of biodiesel. The research stages were: synthesis of methyl ester and its characterization, and corrosion test. The results showed that the methyl ester characteristics of the samples meet requirements with SNI7182 : 2015. In GC-MS results, the largest components of methyl esters from candlenut oil and sunflower seed oil were 35.04% methyl oleate and 46.79% methyl oleate respectively, while in palm oil, the largest components were 41.60% methyl oleate and 41.16%. methyl palmitate. Corrosion test showed that the corrosion rate of ferrous nail in biodiesel at room temperature was lower than 70 ℃. Based on GC-MS and SEM results, biodiesel contained high unsaturated fatty acids and had a corrosion rate, i.e., at room temperature, the methyl ester of palm oil, candlenut oil, and sunflower seed oil were 0.006 mpy, 0.011 mpy, and 0.011 mpy respectively, while at 70 ℃, they were 0.011 mpy, 0.016 mpy, and 0.017 mpy, respectively. The results corresponded to SEM results at high temperature and significantly high content of unsaturated fatty acids. It was indicated by the formation of pits.</p> </abstract>