1. The seasonal trend of certain chemical constituents of established big bluestem (A. furcatus) and needle grass (S. spartea) growing on lowland and upland habitats in eastern Nebraska was studied. The effects of periodic clipping on the composition of established and seedling plants were also investigated. 2. The growing season was characterized by a late spring; sufficient rainfall in April, May, and June; and severe drought in July and August. Mean monthly temperatures were all above normal. After late June little soil moisture available for growth occurred on the upland. On the lowland, water remained available, at least in small amounts, throughout the investigation. 3. Established big bluestem plants on the lowland became taller than those on the upland. The latter showed severe drought effects early in July, and flowering occurred only in the lowland plants. The degree of development of needle grass in the two habitats was similar, although more drying of foliage occurred in plants on the upland. During the flowering stage, grasses with flower stalks and grasses remaining in the leaf stage were separately collected. Established grasses on the lowland regenerated after five cuttings. Seeds planted on the upland and lowland produced seedlings, which were clipped from one to three times. 4. The sugar plus starch percentage was greater in big bluestem on the upland than on the lowland early in the season, but later the situation was reversed. The percentage decreased in big bluestem on the upland when the leaves began to dry up. This drying may have reduced photosynthesis and may have permitted the soluble sugars to be leached from the leaves. The hemicellulose percentage in big bluestem was usually higher in established plants on the lowland, perhaps because of their more stemmy nature. Needle grass on the upland, however, had a higher percentage of hemicellulose than lowland plants. Indications were that hemicellulose is not used as a reserve food. 5. Varying available water content between the upland and the lowland was probably the primary factor in causing the established unclipped plants in the two habitats to be chemically different, since the supply of available water was probably the most critically controlling factor in determining the degree of development and drying of the plants. 6. Clippings from established plants which were periodically clipped were succulent, and their percentages of soluble nitrogen and "true" protein were high, but their percentage content of hemicellulose was low. Clippings from seedlings showed generally similar results. 7. The soluble nitrogen, "true" protein, and total ash percentages were higher in grass remaining in the leaf stage than in plants with flower stalks, but the sugar-starch percentage was greater in the culms. 8. This investigation may be of value in comparing the forage value of big bluestem and needle grass in different habitats and under grazing.