Essential Trace Element Research Articles

Assessment of Essential Trace Elements Cu, Zn, Mn, and Ni in Mediterranean Mussels (Mytilus galloprovincialis) from the Algerian Coast.

The aim of this study was to determine the levels of essential trace elements copper, zinc, manganese, and nickel in two size groups of mussels, Mytilus galloprovincialis, sampled from three sites along the Algerian coast with different levels of pollution. The results of this work are very interesting for determining the effect of mussel size on the variation of contaminant levels in environmental monitoring studies. Thirty individuals from 2 size groups, less than 4-cm and more than 4-cm shell length, were collected over 4 seasons at each site studied. The samples were dissected, and copper, zinc, manganese, and nickel were measured in the whole soft tissues by inductively coupled plasma mass spectrometry (ICP-MS). The maximum concentrations of copper, zinc, manganese, and nickel expressed in mg/kg dry weight at the three sites were 54.01, 146.25, 20.69, and 8.85, respectively. A clear distribution pattern of the studied elements was observed in the two size classes. In fact, significantly higher concentrations (P < 0.05) were found in individuals with a shell length of less than 4 cm. Significantly, higher concentrations (P < 0.05) were found in individuals with a shell length of less than 4 cm due to their high filtration and low excretion activities compared to long individuals. In addition, metal pollution index (MPI) values were higher in small bivalve individuals. The trace element contents found in the mussel samples of the present study showed large spatial and temporal variations among the different sites studied, highlighting the typical exposure of marine sites to different marine pollutants. Notably, elevated concentrations of copper were observed in the port of Zemmouri, likely due to port activities. Conversely, the highest levels of manganese and nickel were recorded at Boudouaou El Bahri, potentially attributable to the use of fertilisers by agricultural activities. Some shellfish samples had exceeded the permissible concentration levels of copper and manganese, thereby posing a danger to bivalve consumers.

Electrochemical sensing of vitamin B6 (pyridoxine) by adapted carbon paste electrode

The recent investigation targets to use adapted carbon paste (CP) with copper nanoparticles (CuNs) operating in a phosphate buffer (PBS) medium with a pH range of 5.0–8.0, to synthesize a novel, susceptible, and simple electrochemical sensor for the detection of one of the most important drugs, vitamin B6. Copper (Cu) is one of the most three common essential trace elements found in the bodies of both humans and animals, along with iron and zinc for all crucial physiological and biochemical functions. Its properties, which are assessed using a variety of methods including scanning electron microscopy (SEM), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS), have also drawn a lot of attention recently. We considered the effects of pH, buffer, scan rate, interference, and calibration curve. The susceptible electrode's linear calibration curve encompassed concentration values between 8.88 and 1000.0 µM. The calculated limits of detection and quantification were 32.12 and 107.0 µM, respectively. Furthermore, this method was established in real human urine samples and drug validation which have been shown satisfactory results for vitamin B6 detection.

Chemical speciation of essential trace elements in edible bamboo shoots from different varieties and sampling locations by HPLC – ICP-MS and HPLC - electrospray MS/MS

Samples of five different commonly consumed bamboo species collected at five different locations in the State of Manipur, northeast India, were investigated for the concentrations and, for the first time, for the chemical forms of essential trace elements. Several elements were determined in the bamboo shoots of which Fe, Zn, Cu, Mn, and Ni were present above the 1 ppm level in bamboo juice. An analysis of data for the variability using the ANOVA method, indicated no statistically significant effect of the geographical location on the trace element pattern and speciation. However, nickel and, to lesser extent, zinc concentrations and speciation were affected by the bamboo variety. Speciation revealed the presence of metal complexes with nicotianamine (NA), deoxymugineic acid (DMA) and histidine.

Construction of novel π-bridged fluorescent probes for Fe2+ monitoring in living cells and foods.

Fe2+ plays a crucial role in biological systems as an essential trace element in the body. Iron is absorbed by the body through food and Fe2+ is also an important component of living cells; therefore, quantitative testing of Fe2+ in food and in living cells is of great importance. This paper presents the development of a novel π-bridge EDOT-based N-oxide turn-on fluorescent probe, designated as FeE, for the detection of Fe2+. The probe has been shown to exhibit excellent sensitivity, a favorable linear relationship between fluorescence signal intensity and Fe2+ concentration, and an effective immunity to interference. The probe has low cytotoxicity and has been successfully used to detect Fe2+ in cells using laser confocal fluorescence microscopy. It is also possible to determine the presence of Fe2+ in animal blood, spinach, apple juice, red wine, mineral water and metal cans.

Plasma Zinc Levels in Patients with Diabetic Nephropathy: Is there a Relationship with NLRP3 Inflammasome Activation and Renal Prognosis?

Zinc is an essential trace element, and impaired zinc homeostasis may be associated with inflammation in patients with diabetic nephropathy (DN). We investigated the influence of zinc level on nod-like receptor nucleotide-binding domain and leucine-rich repeat pyrin-3 domain (NLRP3) inflammasome expression and renal prognosis in patients with DN. We recruited 90 patients definitively diagnosed with DN by renal biopsy and 40 healthy controls. Zinc, NLRP3, interleukin (IL)-1β, and IL-18 levels were detected in blood samples, and the correlations between these parameters were assessed. Receiver operating characteristic (ROC) curve and decision curve analysis (DCA) evaluated the predictive value of zinc and the NLRP3 inflammasome for DN. Furthermore, patients with DN were divided into low- and normal-zinc groups to observe differences in clinical indicators and identify expression of inflammatory-related factors in renal tissue. Kaplan-Meier survival curves predicted the impact of zinc levels on renal prognosis. We found thatthe plasma zinc concentration in patients with DN was lower, while NLRP3, IL-1β, and IL-18 levels were higher than were those in patients without DN (P < 0.05). Zinc level was negatively correlated with NLRP3, IL-1β, and IL-18 levels (P < 0.01). Zinc and the NLRP3 inflammasome were predictive of DN, but their combination improved the diagnostic value. The DCA curve demonstrated a good positive net benefit in the combined model. Compared to patients with low zinc levels, patients with normal zinc levels had lower expression of NLRP3 inflammasome and a better prognosis. Zinc has a protective effect on DN and may affect NLRP3 inflammasome activation.

The interplay between oxidative stress, zinc, and metabolic dysfunction in polycystic ovarian syndrome.

Polycystic ovarian syndrome (PCOS) is a functional endocrine disorder characterized by hyperandrogenism, ovulatory dysfunction, and polycystic ovarian morphology that has been associated with chronic disease and comorbidities including adverse metabolic and cardiac disorders. This review aims to evaluate the role of oxidative stress and zinc in the metabolic dysfunction observed in PCOS, with a focus on insulin resistance. Recent studies indicate that oxidative stress markers are elevated in PCOS and correlate with hyperandrogenemia, obesity, and insulin resistance. Zinc, an essential trace element, is crucial for metabolic processes, particularly in the pancreas for beta-cell function and glucagon secretion. Insufficient zinc levels have been linked to diabetes, obesity, and lipid metabolism disorders. This review aims to highlight the interplay between oxidative stress, zinc, and metabolic dysfunction in PCOS, suggesting that zinc supplementation could mitigate some metabolic and endocrine manifestations of PCOS.

PEG-SeNPs as therapeutic agents inhibiting apoptosis and inflammation of cells infected with H1N1 influenza A virus

The rapid variation of influenza challenges vaccines and treatments, which makes an urgent task to develop the high-efficiency and low-toxicity new anti-influenza virus drugs. Selenium is one of the essential trace elements for the human body that possesses a good antiviral activity. In this study, we assessed anti-influenza A virus (H1N1) activity of polyethylene glycol (PEG)-modified gray selenium nanoparticles (PEG-SeNPs) on Madin-Darby Canine Kidney (MDCK) cells in vitro. CCK-8 assay showed that PEG-SeNPs had a protective effect on H1N1-infected MDCK cells. Moreover, PEG-SeNPs significantly reduced the mRNA level of H1N1. TUNEL-DAPI test showed that DNA damage reached a high level but effectively prevented after PEG-SeNPs treatment. Meanwhile, JC-1, Annexin V-FITC and cell cycle assay demonstrated the apoptosis induced by H1N1 was reduced greatly when treated with PEG-SeNPs. Furthermore, the downregulation of p-ATM, p-ATR and P53 protein, along with the upregualation of AKT protein indicated that PEG-SeNPs could inhibit H1N1-induced cell apoptosis through reactive oxygen species (ROS)-mediated related signaling pathways. Finally, Cytokine detection demonstrated PEG-SeNPs inhibited the production of pro-inflammatory factors after infection, including IL-1β, IL-5, IL-6, and TNF-α. To sum up, PEG-SeNPs might become a new potential anti-H1N1 influenza virus drug due to its antiviral and anti-inflammatory activity.

Exploring serum trace element shifts: Implications for cervical intraepithelial neoplasia

BackgroundCervical intraepithelial neoplasia (CIN) represents a premalignant state presumably related to perturbations in circulating levels of trace elements. Materials and methodsEmploying inductively coupled plasma mass spectrometry (ICP-MS), we quantified essential and toxic trace elements in the sera of 60 women diagnosed with CIN and 60 age-matched healthy counterparts. ResultsOur investigation revealed a noteworthy higher levels in serum of Mn, Zn, and Pb, as well as lower levels in Ni, Se, Rb, and Mo levels within the CIN cohort. Levels of Mn, Zn, and Pb were higher by approximately 5.5-fold, 3.0-fold, and 7.5-fold, respectively, while Mo levels exhibited an approximate 4.5-fold reduction in CIN sera compared to the control group. While the study provided valuable insights into trace element variations, it’s important to note that the adult Serbian population is considered Zn-deficient, so the Zn data should be interpreted with caution. Age stratification (30–40 vs. 40–50 vs. 50–60 years), smoking status (smokers vs. nonsmokers), and CIN severity (CIN 2 vs. CIN 3) yielded no significant disparities in elemental profiles. Among the 10 proposed ratios, 5 demonstrated a significant surge in CIN sera relative to controls: Mn/Se, Mn/Mo, Zn/Se, Zn/Mo, and Se/Mo. Correlation analysis of trace element levels revealed a predominantly consistent pattern between CIN cases and healthy subjects, except for Zn and its negative correlations (antagonistic interactions) with other analyzed trace elements. ConclusionOur findings underscore differences in serum levels of specific trace elements in CIN cases versus controls, implicating their potential involvement in the underlying pathophysiological cascades culminating in cervical neoplasms.

A Novel Manganese Ion Delivery Carrier Promotes Immune Cell Proliferation and Enhances Innate Immune Responses.

Manganese is a transition metal that is an essential trace element for human health. Manganese ions (Mn2+), which serve as one of the most common transition metal ions, play vital roles in enhancing innate immune responses. However, immune agonists based on Mn2+ are poorly utilized in clinical trials due to poor chemodynamics and adverse events. In this work, we designed a novel delivery carrier for loading manganese ions by constructing hFn-MT3(Mn2+) protein nanoparticles (termed as NPs(Mn2+)), which contained human ferritin heavy chain (hFn) and metallothionein-3 (MT3), induced by isopropyl β-d-thiogalactoside (IPTG) and manganese ions in the prokaryotic expression system. The NPs(Mn2+) protein nanoparticles could not only stimulate immune cell proliferation but also activate innate immune responses via the cGAS-STING-IRF3 signaling pathway. Collectively, our results unveil a candidate strategy for delivering metal ions beyond Mn2+ and may broaden metal ion clinical use in the field of immunotherapies.

Metabolic engineering of Escherichia coli for seleno-methylselenocysteine production

Selenium (Se) is an essential trace element for life. Seleno-methylselenocysteine (SeMCys) can serve as a Se supplement with anticarcinogenic activity and can improve cognitive deficits. We engineered Escherichia coli for microbial production of SeMCys. The genes involved in the synthesis of SeMCys were divided into three modules–the selenocysteine (SeCys) synthesis, methyl donor synthesis and SMT modules–and expressed in plasmids with different copy numbers. The higher copy number of the SeCys synthesis module facilitated SeMCys production. The major routes for SeCys degradation were then modified. Deletion of the cysteine desulfurase gene csdA or sufS improved SeMCys production the most, and the strain that knocked out both genes doubled SeMCys production. The addition of serine in the mid-logarithmic growth phase significantly improved SeMCys synthesis. When the serine synthetic pathway was enhanced, SeMCys production increased by 12.5 %. Fed-batch culture for sodium selenite supplementation in the early stationary phase improved SeMCys production to 3.715 mg/L. This is the first report of the metabolic engineering of E. coli for the production of SeMCys and provide information on Se metabolism.

Comparative study of serum zinc levels in stable asthmatic versus non-asthmatic children

Objective: Zinc is an essential trace element that plays a critical role in various physiological processes, including immune function and antioxidant defense. Serum zinc levels are often used as a marker to assess nutritional status and potential deficiencies. This study aims to compare serum zinc levels between stable asthmatic children and non-asthmatic controls at ESUT Teaching Hospital, Parklane Enugu, to understand whether asthmatic children have different zinc status compared to their non-asthmatic counterparts. Materials and Methods: A comparative cross-sectional study was conducted involving 85 asthmatic and 85 non-asthmatic children aged 5–11 years. Serum zinc levels were assessed using a Flame Atomic Absorption Spectrophotometer (FAAS). Data were analyzed using SPSS version 20. Results: The median serum zinc levels were 11.95 µmol/L in asthmatic children and 8.81 µmol/L in non-asthmatic children (p &lt; 0.001). Nutritional status and socio-economic class did not significantly affect serum zinc levels. Conclusion: Stable asthmatic children have higher median serum zinc levels compared to non-asthmatic children. There was no significant relationship between serum zinc levels and socio-economic or nutritional status in the asthmatic group. Blind zinc supplementation in asthmatic children should be approached with caution.

Arsenic, selenium, and mercury speciation in hypersaline lakes of the Andean Altiplano: Link between extreme levels and biodiversity repartition

Arsenic (As) and mercury (Hg) are highly toxic contaminants whereas selenium (Se) is both an essential trace element and potentially harmful at higher concentrations. The hyper-saline lakes of southern Bolivian Altiplano, which are ecological niches for endemic species, are also expected to be enriched in these toxic trace elements. The biogeochemistry of As, Hg, and Se in such high-altitude extreme environments (e.g., high UV radiation and salt content) remains poorly understood. In this study, we investigated the concentrations and chemical forms (speciation) of As, Hg, and Se in sediment, water, and air samples of Lagunas Colorada (LC), Verde (LV), and Blanca (LB) in the South Lipez region (>4200 m a.s.l.). We compared them with the repartition of biodiversity (invertebrates, algae, and bacteria). Extreme As concentrations were found in water (up to 82 mg L−1), and the main As species was inorganic As(V), with neither biogenic methylated As nor volatile As forms being detected in water and air, respectively. Se concentrations in water were of 0.1 to 1.4 μg L−1, and Se existed under different redox states, i.e., Se(IV), Se(VI), and reduced Se (0, -II), including biogenic methylated Se(-II) (trimethyl selenonium). Volatile Se compounds (e.g., dimethyl selenide) were detected in water and air samples. Hg was enriched in the surface water (6 to 30 ng L−1) compared to other regional water bodies, and a significant amount of methyl-Hg and gaseous Hg(0) was detected. The drastic disparity between As, Se and Hg concentrations and speciation between lakes has important implications for their cycling in these extreme aquatic systems. While As mostly accumulated in its oxidized and non-volatile form, Hg and Se concentrations can be controlled by significant conversion to reduced and methylated forms, allowing efficient evasion to the atmosphere. Finally, the salinity, including major ions, and high levels of As were among the main drivers of biodiversity repartition between lakes.

Acute Toxicity of Zinc Sulphate (ZNSO4) on the Estuarine Blood Clam Anadara granosa (Linnaeus, 1758)

Zinc is an essential trace element for taking role in electron transfer in many enzymatic reactions. However, its prolonged and excessive intake may lead to toxic effect. Aquatic organisms take up zinc in proportion to the dissolved concentration of the metal in water. Generally, uptake occurs throughout epithelial surfaces related to absorption and excretion of ions such as the gill membranes. Heavy metals can accumulate in the tissues of aquatic organisms and cause a range of hazardous effects to all organisms through bio-magnifications. There are a number of studies carried out on the toxicity of Zinc sulphate. The aim of this study was to investigate the acute toxicity effects of Zinc sulphate on the clam Anadara granosa. Clam samples were exposed to different concentrations (3, 6, 9, 12, and 15.00 mg/L) of Zinc sulphate for 96h and their cumulative mortality was calculated in 12 hours intervals. Results were analyzed by SPSS 20 to obtain number of cumulative mortality and lethal concentrations. The blood clam Anadara granosa LC50 and LC90 for zinc sulphate at 96 hours were 4.63 and 9.28mg/L in the current study. Mortality rate was increased with the increase in metal concentrations and time.

A rare case of early onset lewy body dementia with parkinsonism associated with chronic exposure to copper contaminated drinking water.

There is a well-recognized relationship between a person's body burden of essential trace elements such as copper and their neurological function in which both deficiencies and exposures to excessive concentrations are associated with adverse clinical outcomes. Preclinical studies indicate chronic excess copper exposure is associated with altered motor function, dopaminergic neuronal loss, astrocytosis, and microgliosis. Copper also promotes oligomerization and fibrilization of α-synuclein suggesting it may hasten the course of an α-synucleinopathy. Here we report a rare case of early onset Lewy Body Dementia with Parkinsonism in a 53-year-old Caucasian woman exposed to copper contaminated drinking water for more than 10 years. Her hair and that of her daughter had streaks of blue-green discoloration as did the porcelain sinks in their home. Testing confirmed copper contamination of the drinking water. A neurologist diagnosed her with Lewy Body Dementia with Parkinsonism. Skin biopsy for phosphorylated α was consistent with a diagnosis of an α-synucleinopathy. These findings suggest chronic exposure to excessive copper may act as disease modifying factor in Lewy Body Dementia with Parkinsonism. It has previously been recommended that individuals at risk of Alzheimer's disease (AD) avoid excessive intake of copper. Genetic studies indicate that Lewy Body Dementia shares risk factors and pathways with AD. Based on the observations in this patient we recommend that individuals at risk for an α-synucleinopathy based on a positive family history, genetic testing, and/or positive results on a skin biopsy for phosphorylated α-synuclein avoid exposure to excess copper.

Advances in the Synthesis and Bioactivity of Polysaccharide Selenium Nanoparticles: A Review.

Selenium, an essential trace element of the human body, is pivotal in human health and disease prevention. Nevertheless, the narrow therapeutic index of selenium, where the toxic and therapeutic doses are close, limits its clinical utility. Significantly, nanoscale selenium synthesized by different methods using polysaccharides as stabilizers has low toxicity properties and exhibits excellent bioactivity. Its biological activities, such as anti-tumor, anti-inflammatory, antioxidant, antibacterial, and immune function enhancement, are improved compared with traditional organic and inorganic selenium compounds, conferring greater potential for application in biomedicine. Therefore, this review evaluates the advancements in various synthesis methodologies for polysaccharide selenium nanoparticles (Se NPs) and their biological activities. It aims to provide a comprehensive theoretical basis and research directions for the future development of highly efficient, minimally toxic, and biocompatible polysaccharide-Se NPs and the application of polysaccharide-Se NPs in biomedicine.

Food Waste Processing Trends Worldwide and Valorization of Food Waste from Pune City

This article lays its primary focus on understanding waste from the domestic and commercial hotel kitchens. For researchers, deciding biotechnological treatment and valorization process, it is necessary to refer and understand the food waste (FW) composition and its processing trends worldwide. This paper mentions the FW compositional data from four different locations of “Pune” a metro city of India. Study-approved procedures for sample collection, preparation, and analysis were followed. The results indicate, the presence of carbohydrates, proteins, and fats in all samples and are sufficient to support the microbial growth and desired product formation. Being perishable, microbiological and compositional changes during handling and transportation, in FW are inevitable. The presence of high boiling, low boiling volatile compounds and volatile organic acids indicate the complexity of FW and microbial activity taking place within it. Abundant amount of trace elements essential for enzymatic reactions and building block molecule formation are present. Average important figures to mention, starch- 7.27% w/w, free sugars- 3.39% w/w, proteins- 7.99% w/w and fats- 12.84% w/w. In addition, essential trace elements Zn- 4 mg/lit, Mn- 4 mg/lit, Mg- 239 mg/lit, S-433 mg/lit and P- 922 mg/lit. The numbers help to realize the nutritional richness of the FW in reality and provide fundamental statistical data for researchers. Treatment and recycling can be done to contribute toward a circular bio-economy. Remarkable variations observed in almost all components on day-to-day basis. However, pretreatment process parameters can be decided to account for compositional variations.

Investigation of the concentration and isotopic composition of Cu, Fe and Zn in human biofluids in the context of Alzheimer’s disease via tandem and multi-collector inductively coupled plasma-mass spectrometry

Studies on essential trace elements in the context of Alzheimer’s disease (AD) concluded that Cu, Fe and Zn interact with amyloid-β, accelerating plaque formation in the brain. Additionally, Cu and Fe in the vicinity of plaques produce reactive oxygen species (ROS) resulting in oxidative stress, whereas Zn plays a role in the antioxidant defence as a co-factor for antioxidants. In this work, the Cu, Fe and Zn concentrations and isotope ratios were determined in whole blood, blood serum and cerebrospinal fluid of 10 patients diagnosed with AD and 8 control individuals, using tandem (ICP-MS/MS) and multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS), respectively. In whole blood and blood serum of AD patients, a heavier Cu isotopic composition was observed (significant for whole blood only) compared to controls. Albumin levels in cerebrospinal fluid tend to increase with age, which could indicate an increased leakiness of the blood-brain barrier. In cerebrospinal fluid, a large variability was observed for the Cu and Fe isotope ratios, potentially resulting from that leakiness at the blood-brain barrier. Therefore, potential effects of AD on the concentration and isotopic composition of essential elements in cerebrospinal fluid related to amyloid-β formation could be hidden. Finally, in blood serum, Zn, urea and creatinine concentrations showed an increase with age and showed a significant difference between sexes.

Effect of Selenium and Selenoproteins on Radiation Resistance.

With the advancement of radiological medicine and nuclear industry technology, radiation is increasingly used to diagnose human health disorders. However, large-scale nuclear leakage has heightened concerns about the impact on human organs and tissues. Selenium is an essential trace element that functions in the body mainly in the form of selenoproteins. Selenium and selenoproteins can protect against radiation by stimulating antioxidant actions, DNA repair functions, and immune enhancement. While studies on reducing radiation through antioxidants have been conducted for many years, the underlying mechanisms of selenium and selenoproteins as significant antioxidants in radiation damage mitigation remain incompletely understood. Therefore, this paper aims to provide new insights into developing safe and effective radiation protection agents by summarizing the anti-radiation mechanism of selenium and selenoproteins.

Therapeutic Efficacy of Selenium Pre-treatment in Mitigating Cadmium-Induced Cardiotoxicity in Zebrafish (Danio rerio)

Cardiovascular diseases are a rampant public health threat. Environmental contaminants, such as Cadmium (Cd), a toxic metal, are risk factors for cardiovascular diseases. Given that human exposure to Cd is increasing, there is a need for therapies to ameliorate Cd toxicity. Selenium (Se), an essential trace element, has been proposed to rescue the effects of Cd toxicity, with mixed effects. Se's narrow therapeutic window necessitates precise dosing to avoid toxicity. Here, we assessed the effects of various waterborne Cd and Se concentrations and sequences on cardiac function using zebrafish (Danio rerio). We showed that Cd induced pericardial edemas and modified heart rates in zebrafish larvae in a concentration-dependent manner. To identify the therapeutic range of Se for Cd-induced cardiotoxicity, zebrafish embryos were treated with 0, 10, 50, 100, 150, or 200 μg/L Se for 1–4 days prior to exposure to 2.5 and 5 μg/L Cd. We found that a 50 µg/L Se pre-treatment before 2.5 μg/L Cd, but not 5 μg/L Cd, reduced the prevalence of pericardial edemas and ameliorated Cd-induced bradycardia in zebrafish. Zebrafish exposed to 10 and 50 μg/L of Se for up to 4 days showed typical heart morphology, whereas other Se-exposed and control fish presented pericardial edemas. Longer Se pre-treatment durations led to fewer incidences of pericardial edemas. Overall, this study highlights the importance of optimizing Se concentrations and pre-treatment periods to harness its protective effects against Cd-induced cardiotoxicity. These findings provide insights into potential therapeutic strategies for reducing Cd-related cardiovascular damage in humans.Graphical

Buckwheat: An Underutilized Himalayan Crop with Multifaceted Nutraceutical Benefits.

The human population is growing and alternate food options are needed to provide food and nutritional security to mankind. Reduced agricultural output as a result of climate change and increased demand for grains because of continuous population growth have created a gap between demand and supply of food. Buckwheat is a pseudocereal crop plant with high nutritional value that can be included as an alternate food in our diet. It is a traditional crop plant grown in the high mountains of the Himalayas for food as well as fodder. It completes its life cycle in 3-4 months, so is mostly grown as a second crop in between main crops like maize and barley. It also acts as a green manure by improving the phosphorus content of the soil. Buck-wheat has high nutritional value as it is rich in essential amino acids, vitamin B, trace elements, and other nutrients. The main bioactive compounds identified in buckwheat are rutin, quercetin, isoquercetin, d-chiroinositol, resveratol, and vitexin, which are responsible for its pharmacolog-ical properties. Research focused on value addition by exploring its nutritional, pharmaceutical, and other alternative uses of commercial importance, is needed for reviving buckwheat cultiva-tion practices and its conservation. Considering the multifarious applications of buckwheat, this review summarizes the currently available knowledge on the agronomic and nutraceutical sig-nificance of buckwheat to project its value as a future crop in the avenue of agriculture and functional food.