Plant Essential Oils Research Articles

Plant extracts for controlling the mosquito vector in the Amazon region

Mosquito-borne diseases continue to be the main causes of death in many tropical and sub-tropical countries. Among the infectious diseases transmitted by mosquitoes, malaria remains a major public health concern. Chemical control is the most widely used method to control and prevent mosquito-borne diseases. However, there are reports in the literature about the resistance of populations due to continuous use. In this sense, products of plant origin, such as plant extracts, essential oils and plant derivatives, have emerged as promising alternatives. In this study, bioassays were carried out to evaluate the activity of the aqueous and ethanolic extracts of cloves in laboratory conditions on Anopheles sp. larvae. The bioassays carried out on An. darlingi showed CL50 values of 227.29 µg/mL for the ethanolic extract and CL50 values of 263.60 µg/mL for the aqueous extract. the study presented important data on the activity of the aqueous and ethanolic extracts of cloves.

Monitoring, antimicrobial activity evaluation, and molecular docking studies of chemical composition of various plant species to identify an inhibitory drug for Clostridium perfringens bacteria isolated from drinking water resources in Ardabil province

In this study, both the in vitro antimicrobial activity of plant essential oils and their active ingredients and their molecular docking properties are evaluated and a comprehensive understanding of their potential as therapeutic agents is provided. The study findings revealed that the bacterium C. perfringens was detected in 32.5 % of the drinking water sources. Regarding MIC, the essential oils of thymus, hypericum perforatum, zingiber officinale, foeniculum vulgare, and malva were identified at concentrations of 20 %, 35 %, 25 %, 20 %, and 30 %, respectively, for combating C. perfringens. In terms of MBC, the essential oils of thymus, hypericum perforatum, zingiber officinale, foeniculum vulgare, and malva at concentrations of 25 %, 45 %, 35 %, 30 %, and 40 % respectively, were found to deactivate the bacterium. Based on the results of the antimicrobial activity assessment, molecular docking studies, and evaluation of physicochemical properties, it can be inferred that the compounds from the essential oils of thymus (including linalool and thymol), foeniculum vulgare (including anethole), and zingiber officinale (including zingerone) could be promising candidates for inhibiting C. perfringens compared to the reference drugs metronidazole and ciprofloxacin. On the other hand, molecular docking studies show that the active ingredients anethole, zingerone, thymol and linalool with more potent binding energy than the reference drug metronidazole show better activity against C. perfringens bacteria, which is consistent with the results of antimicrobial activity.

Transcriptome analysis of Reticulitermes flaviceps exposed to Mentha spicata essential oil and carvone indicates a potential neurotoxic mechanism of action characterized by intracellular calcium influx mediated by dopamine receptors

Reticulitermes flaviceps is an economically important pest in agriculture, forestry, and construction. Recent studies have shown an increase in research focusing on the anti-termite properties of plant essential oils, however, there remains a lack of information regarding the specific molecular mechanism involved. In this study, RNA-seq analysis was conducted on termites exposed to Mentha spicata essential oil (EO) and carvone, leading to the discovery of various genes that were expressed differentially under different treatment conditions. Numerous genes that exhibited a response to M. spicata EO and carvone found to be associated with stress-related pathways, such as drug metabolism cytochrome P450, glutathione metabolism, fatty acid metabolism, citric acid cycle, neuroactive ligand-receptor interaction, cell apoptosis, the AMPK signalling pathway, the mTOR signalling pathway, the longevity regulation pathway, ubiquitin-mediated protein hydrolysis, and the calcium signalling pathway. The up-regulation of genes (SPHK) associated with calcium channels, such as SPHK, indicates a potential mechanism of neurotoxicity, while the up-regulation of apoptosis-associated genes, including ACTB_G1, PYG, SQSTM1, RNF31, suggests a potential mechanism of cytotoxicity. The metabolism of M. spicata EO induces oxidative stress, elevates free Ca2+ levels in mitochondria, and initiates the generation of reactive oxygen species (ROS), ultimately resulting in programmed cell necrosis and apoptosis, as well as facilitating cellular autophagy. The monoterpenes exhibited neurotoxic and cytotoxic effects on R. flaviceps and could be exploited to advance termiticide development and eco-friendly termite control.

Silicon: A Powerful Aid for Medicinal and Aromatic Plants against Abiotic and Biotic Stresses for Sustainable Agriculture

Silicon plays a crucial role in enhancing plant tolerance to various abiotic and biotic stresses, including drought, salinity, heavy metals, and pathogen/pest attacks. Its application has shown promising results in improving stress tolerance and productivity in medicinal plants. This review synthesizes findings from numerous studies investigating the mechanisms by which silicon confers stress tolerance, including the regulation of antioxidant systems, water relations, nutrient homeostasis, phytohormone signaling, and stress-responsive gene expression. Additionally, it examines the effects of silicon supplementation on the production of valuable secondary metabolites and essential oils in medicinal plants. Silicon application can significantly mitigate stress-induced damage in plants, including medicinally important species such as borage, honeysuckle, licorice, Damask rose, savory, basil, and eucalyptus. The deposition of silicon in cell walls provides physical reinforcement and acts as a barrier against pathogen invasion and insect herbivory. Furthermore, silicon fertilization can enhance the production of valuable secondary metabolites in medicinal crops under stress conditions. The findings underscore the potential of silicon fertilization as a sustainable strategy for improving the productivity and quality of medicinal crops under changing environmental conditions, highlighting the need for further research to elucidate the molecular mechanisms underlying silicon-mediated stress tolerance and practical applications in medicinal plant cultivation.

Development of Prangos ferulacea essential oil based nanoemulsions/pickering emulsion and examination of their performance in mayonnaise shelf life

The increasing demand to replace chemical antimicrobial agents with natural compounds leads to the use of some plant extracts and essential oils (EOs) as natural preservatives. Nanoemulsion (NE) with Tween 80 as wall material and Pickering emulsions (PE) stabilized with carbohydrate (Arabic gum (AG)) or protein (sodium caseinate (SC)) wall materials. First of all, the PEO was obtained from two parts of plant: flowers and leave, and characterized by GC-MS and the IC50 index measurement. The Prangos ferulacea essential oil (PEO) extracted from leave was selected for the next tests due to slightly higher antioxidant activity compared to the flower's EO. The leave's PEO was encapsulated in three forms: nanoemulsion (NE) with Tween 80 as wall material and Pickering emulsions (PE) stabilized with carbohydrate (gum Arabic (GA)) or protein (sodium caseinate (SC)) wall materials. The NE had the smallest particle size and the highest antioxidant and antimicrobial (S. aureus and E. coli) activity. AG-stabilized PE recorded the highest encapsulation efficiency (85.37 ± 1.97 %). The occurring of new chemical interactions between PEO and wall materials and uniformly shaped PE microcapsules were approved by FT-IR and FE-SEM tests, respectively. Free and encapsulated PEO were added to the mayonnaise, and the samples were analyzed during storage at 4 °C for 60 days. Peroxide value and TBA index gradually increased for all mayonnaise samples, but this increase was lower for samples containing NE and SC-stabilized PEO capsules. In general, carriers stabilized with the simultaneous application of protein and carbohydrate performed better compared to their single application in sensory tests in mayonnaise.

Soil sorption and effects on soil microorganisms of thymol and carvacrol monoterpenes from essential oils of aromatic plants

Soil sorption and effects on soil microorganisms of thymol and carvacrol monoterpenes from essential oils of aromatic plants

Borneol essential oil: Enzyme-assisted extraction, inhibitory effect on Propionibacterium acnes, and study on acne treatment mechanism based on network pharmacology-molecular docking

Borneol essential oil (BEO), a plant essential oil derived from hydrodistillation of fresh branches and leaves of Cinnamomum camphora (L.). This study optimized cellulase and xylanase-assisted BEO, extraction using an orthogonal design. Additionally, it explored the inhibitory effects of BEO on Propionibacterium acnes and elucidated the anti-bacterial mechanisms via molecular docking. Furthermore, the acne treatment mechanism of BEO was investigated using network pharmacology-molecular docking. The findings indicated that the concentration of borneol in BEO was 2.34 times higher than that achieved through hydrodistillation. This enhancement was achieved by adding enzymes in a volume equivalent to 2 % of the raw material, followed by a 2-hour enzymolysis process conducted at 40℃. BEO exhibited a significant inhibition zone of 31.6±0.5 mm against Propionibacterium acnes. Molecular docking analysis revealed that six, three, and one chemical components in BEO exhibited molecular binding energies exceeding 5.00 kJ/mol with key target proteins involved in the pathogenesis of Propionibacterium acnes: deoxyribonuclease I (DNase I), dermatan sulfate (DsA1), and Christie, Atkins, Munch-Peterson (CAMP), respectively. This indicates a close relationship between the chemical components and the target proteins. Network pharmacology analysis identified 40 common targets between BEO and acne formation, from which 12 key targets were identified. Among these, ovarian steroidogenesis exhibited the highest degree of enrichment in the signaling pathway. These findings provide a scientific foundation for the development of acne treatment cosmetics utilizing BEO.

Isolation and identification of postharvest pathogenic bacteria of Syzygium samarangense and antibacterial test of essential oils

With Syzygium samarangense as the test subject，postharvest pathogenic bacteria were isolated，purified，and identified. Their pathogenicity was determined using Koch's postulates，and the antibacterial activity of 36 types of plant essential oils against highly pathogenic bacteria was assessed. The findings indicated that a highly pathogenic fungus was isolated and identified from naturally existing S. samarangense, labeled LW3-1. The pathogenicity rate of strain LW3-1 reached 100% within 2 days of inoculation, and molecular biology identified it as Colletotrichum gloeosporioides. Among the 36 essential oils, clove, geranium, myrrh, rose and sophora flavescens essential oils (1000 μl/L) had the most significant antibacterial effect on the LW3-1 strain, with an antibacterial rate of 100%. The antibacterial rate of 500 μl/L clove essential oil against the LW3-1 strain was still >85% after 12 days of treatment. In summary, it is determined that the pathogenic bacteria causing postharvest diseases of l S. samarangense is C. gloeosporioides, which is highly pathogenic. 500 μl/L clove essential oil has the best inhibitory effect on C. gloeosporioides.

Isolation and identification of postharvest pathogenic bacteria of nectarine and antibacterial test of essential oils

With 'Rose Red' nectarine as the test subject，postharvest pathogenic bacteria were isolated，purified，and identified. Their pathogenicity was determined using Koch's postulates，and the antibacterial activity of 28 types of plant essential oils against highly pathogenic bacteria was assessed.The results showed that a highly pathogenic pathogenic fungus TBZ1-9 was isolated and identified from peach fruits and had a high genetic relationship with Fusarium oxysporum. Among the 28 essential oils, mustard and Achyranthes essential oil (1000 μl/L) had the most significant antibacterial effect on strain TBZ1-9, with an antibacterial rate of 100%.500 μl/L Achyranthes essential oil had the best inhibitory effect on the TBZ1-9 strain, and the antibacterial rate was still >85% after 12 days of treatment. In summary, it was determined that the pathogen causing postharvest diseases of nectarines is Fusarium oxysporum, which is highly pathogenic. 500 μl/L Achyranthes essential oil has the best inhibitory effect on F. oxysporum.

Anti-fungal efficacy of Carvacrol against Candida glabrata clinical isolates of vulvovaginal candidiasis

Fungal infections affect over 1 billion people worldwide each year, including superficial infections like athlete's foot and more severe systemic infections. Fungal diseases are responsible for an estimated 1.5 million deaths annually, a figure comparable to or exceeding the mortality rate of diseases like malaria or tuberculosis. The limited arsenal of available antifungal drugs, coupled with the emergence of drug-resistant fungal strains, has increased this concern. Therefore, there is a significant need to explore alternative therapeutics to overcome fungal pathogens. Carvacrol, phenolic monoterpenoids, is present in essential oils of many plants and is known for its biological and pharmacological properties. In the present study, the efficacy of carvacrol was investigated against four Candida glabrata strains isolated from patients of vulvovaginal candidiasis, which have shown varying extents of susceptibility against fluconazole. Carvacrol, a phytoactive monoterpene phenol, has shown a minimum inhibitory concentration (MIC50) ranging from 75 to 125 µg/mL and minimum fungicidal concentration of 150 and 175 µg/mL for all clinical isolates, including wild-type strains. Carvacrol, in combination with fluconazole, has shown a strong synergism against wild type C. glabrata with a FIC index value of 0.156. Preliminary mechanistic investigations unveiled that exposure to carvacrol significantly reduced cell surface hydrophobicity and ergosterol content in all strains. In conclusion, carvacrol holds promising potential as an effective antifungal agent against C. glabrata, which is categorized as high priority in the first fungal pathogen priority list of the World Health Organisation released in 2022 for highlighting priority areas for action, including the development of effective therapeutic solution.

Biocontrol Mechanisms of Three Plant Essential Oils Against Phytophthora infestans Causing Potato Late Blight.

Late blight, caused by the notorious pathogen Phytophthora infestans, poses a significant threat to potato (Solanum tuberosum) crops worldwide, impacting their quality as well as yield. Here, we aimed to investigate the potential use of cinnamaldehyde, carvacrol, and eugenol as control agents against P. infestans and to elucidate their underlying mechanisms of action. To determine the pathogen-inhibiting concentrations of these three plant essential oils (PEOs), a comprehensive evaluation of their effects using gradient dilution, mycelial growth rate, and spore germination methods was carried out. Cinnamaldehyde, carvacrol, and eugenol were capable of significantly inhibiting P. infestans by hindering its mycelial radial growth, zoospore release, and sporangium germination; the median effective inhibitory concentration of the three PEOs was 23.87, 8.66, and 89.65 μl/liter, respectively. Scanning electron microscopy revealed that PEOs caused the irreversible deformation of P. infestans, resulting in hyphal shrinkage, distortion, and breakage. Moreover, propidium iodide staining and extracellular conductivity measurements demonstrated that all three PEOs significantly impaired the integrity and permeability of the pathogen's cell membrane in a time- and dose-dependent manner. In vivo experiments confirmed the dose-dependent efficacy of PEOs in reducing the lesion diameter of potato late blight. Altogether, these findings provide valuable insight into the antifungal mechanisms of PEOs vis-à-vis late blight-causing P. infestans. By utilizing the inherent capabilities of these natural compounds, we could effectively limit the harmful impacts of late blight on potato crops, thereby enhancing agricultural practices and ensuring the resilience of global potato food production.

Repellency, Toxicity, and Chemical Composition of Plant Essential Oils from Myrtaceae against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera Liviidae).

Diaphorina citri Kuwayama (D. citri) is one of the major pests in the citrus industry, which spreads Citrus Huanglongbing disease. It has developed resistance to chemical insecticides. Therefore, searching for greener solutions for pest management is critically important. The main aim of this study was to evaluate the repellent and insecticidal efficacy of essential oils (EOs) from four species of Myrtaceae plants: Psidium guajava (PG), Eucalyptus robusta (ER), Eucalyptus tereticornis (ET), and Baeckea frutescens (BF) against D. citri and to analyze their chemical compositions. GC-MS analysis was performed, and the results indicated that the EOs of PG, ER, ET, and BF were rich in terpenoids, ketones, esters, and alcohol compounds. The repellent rate of all four EOs showed that it decreased with exposure time but increased with the concentration of EOs from 80.50% to 100.00% after treating D. citri for 6 h with four EOs at 100% concentration and decreased to 67.71% to 85.49% after 24 h of exposure. Among the compounds from the EOs tested, eucalyptol had the strongest repellent activity, with a 24 h repellency rate of 100%. The contact toxicity bioassay results showed that all EOs have insecticidal toxicity to D. citri; the LC50 for nymphs was 36.47-93.15 mL/L, and for adults, it was 60.72-111.00 mL/L. These results show that when PG is used as the reference material, the ER, ET, and BF EOs have strong biological activity against D. citri, which provides a scientific basis for the further development of plant-derived agrochemicals.

Preparation of preservation paper containing essential oil microemulsion and its application in maintain the quality of post-harvest peaches

Post-harvest peaches are highly susceptible to fungal infections leading to spoilage. This study investigates the utilization of three edible plant essential oils (EOs) with antifungal properties for the preservation of peaches, along with an evaluation of their preservative efficacy. Initially, GC-MS was employed to identify the primary constituents of the EOs, revealing a predominance of phenols, alcohols, hydrocarbons and aldehydes. Principal component analysis was conducted to compare the chemical compositions of the EOs from the plants used in this study with those obtained from other regions. This analysis facilitates the quality assessment of the plant materials in the later stages of the research. In vitro antimicrobial assays indicated that the three EOs independently exhibited their respective antifungal activities against 7 fungi responsible for peach spoilage. The Response Surface Methodology was then applied to optimize the blend ratio of the composite EOs (CEOs) for maximum antifungal efficacy. Concurrently, this study validated the rationality of a second-order polynomial model that correlates the peach decay index with the concentration of the CEOs. To enhance the bioavailability of the CEOs, a pseudo-ternary phase diagram was employed to formulate the CEOs into a microemulsion. Based on the RSM optimization results for the CEOs, the optimal mass ratio of the components within the microemulsion was determined. Using this microemulsion as a base, preservative films were developed that demonstrated a significant preservation effect on peaches, highlighting the immense potential of this research in the food industry.

Antiviral Effect of Different Essential Oils on Avian Coronavirus.

Plant essential oils (EOs) possess established antimicrobial properties; however, research on their antiviral activity, particularly against avian coronaviruses, remains limited. EOs offer a promising plant-based alternative for viral control, especially in scenarios where conventional chemical use is restricted. This study aimed to evaluate the antiviral effects of six different essential oils derived from Syzygium aromaticum, Origanum vulgare, Cymbopogon martinii, Cymbopogon citratus, Mentha piperita, and Mentha spicata against avian coronavirus (AvCov) at 0.1% and 1% dilutions. The antiviral effects of the EOs were assessed via virus isolation from embryonated chicken eggs, and the ability of the EOs to inhibit AvCoV replication was evaluated. The EOs from Syzygium aromaticum, Origanum vulgare, Cymbopogon martinii, and Cymbopogon citratus completely inhibited AvCov replication at a 1% dilution. Conversely, absent to partial inhibitory effect was observed at the 0.1% dilution for all tested EOs, with O. vulgare derived EO exhibiting the greatest inhibitory effect (over 70%). Notably, EOs from Mentha piperita and Mentha spicata were unable to completely inhibit AvCov at either concentration. Our findings highlight the potent antiviral activity of the EOs from Syzygium aromaticum, Origanum vulgare, Cymbopogon martinii, and Cymbopogon citratus against AvCov at a 1% dilution. The complete inhibition observed for these EOs suggests their potential for AvCov control. However, further research is necessary to elucidate the mechanisms of action, optimize formulations, and evaluate the efficacy against other coronaviruses, including those relevant to human health.

An Online Survey on Usability, Acceptability, Attitude and Knowledge of Herbal and Synthetic Cosmetic Among Sikkimese Population

In recent years, the global cosmetics industry has witnessed a notable shift in consumer preferences towards natural and organic products, leading to an increased demand for herbal cosmetics derived from plant-based ingredients. This research survey presents a comparative analysis of herbal and synthetic cosmetics, exploring distinctions in formulation, efficacy, safety, and environmental impact. Herbal cosmetics incorporate natural elements like plant extracts, essential oils, herbs, and fruits, while synthetic counterparts are developed using chemical compounds in laboratories to replicate specific properties. The main objective is to find out the preferences between herbal and synthetic cosmetics among individuals in Sikkim. The methodology adopted for mini survey was pre-prepared questionnaires were developed by using Google forms and shared through the online platforms such as Mail, Whatapps and facebook. An online survey conducted in different districts of Sikkim revealed that 42% of respondents favoured herbal cosmetics, while 31% preferred synthetic products and 23% had no specific preference. Factors influencing those leaning toward synthetic cosmetics included perceived effectiveness, availability, and affordability. Despite the clear interest and preference for herbal cosmetics, the survey also highlighted limited choice and availability in comparison to synthetic alternatives. This suggests a substantial opportunity for growth in the herbal cosmetic industry, emphasizing the need for increased accessibility to meet the growing demand in Sikkim and beyond. Keywords: Knowledge of Herbal, An Online Survey, Herbal Cosmetics, Acceptability, Usability, Sikkimese People

Biosynthesis of secondary metabolites in aromatic and medicinal plants in response to abiotic stresses: A review

Climate change has massive consequences on non-living factors in the environment, resulting in irregular precipitation, fluctuating atmospheric temperature, and variations in humidity. These changes cause biotic and abiotic stresses; plants must have defense mechanisms to survive. Therefore, plants divert some synthesized energy towards producing numerous plant secondary metabolites (PSMs), viz., flavonoids, alkaloids, and essential oils. These compounds act as protections for the plants, helping them to survive under stressful conditions. Medicinal and aromatic plants (MAPs) are sessile organisms that are not immune to harmful consequences of various abiotic stresses in which the PSMs have an important role in acting against the adverse effects. In this regard, the MAPs have a coherent defense mechanism for abiotic stresses. The secondary metabolites produced by these plants are useful as medicines and aromatic products for humans. However, not all stresses produce high secondary metabolites, as their production is highly specific to certain stresses. This review provides a comprehensive understanding of secondary metabolite production under various stressful conditions, including extreme temperature, drought, water logging, salinity, harmful radiation, elevated levels of ozone and CO2, heavy metals, and agrochemicals on MAPs. Additionally, the production of these compounds can be modified by subjecting plants to various stressors. Many authors have reported on PSMs in MAPs, which need to be well documented and exploited for humankind.

Plant Essential Oil Nanoemulgel as a Cosmeceutical Ingredient: A Review

Essential oils (EOs) are concentrated, hydrophobic volatile compounds derived from different parts of plants. They are recognized for their diverse and versatile functional properties. Approximately 90% of EOs are administered via topical or transdermal routes. However, EOs are susceptible to oxidation, and their high volatility often poses a challenge to the transdermal delivery of their bioactive constituents. Additionally, the direct application of pure EOs on the skin may result in irritating effects. Hence, various novel carrier systems have been explored for the topical application of EOs. Among these, nanoemulgel has received particular attention from the cosmeceutical industry. It is a hybrid technology combining nanoemulsion and a gelling phase, which can enhance the bioadhesivity of EOs, at the same time minimizing their irritating effects. This review summarizes the methods of EO extraction, steps and factors influencing the preparation of EO nanoemulgel, and characterization parameters for nanoemulgel studies. The potential cosmeceutical applications of EO nanoemulgels as an anti-inflammatory, antimicrobial, antioxidant, and penetration enhancer are also compiled and discussed.

Bio-fertilizer as a pathway to minimize nitrate leaching from chemical fertilizer in high yield peppermint production

The contamination of Iran's underground water sources with nitrate from chemical fertilizers poses a significant health risk to communities reliant on this essential water source. This study investigates the benefits of organic alternatives, such as vermicompost and bio-fertilizers, in sustainable peppermint production. The aim is to foster environmentally friendly agricultural practices and enhance soil health. Multiple fertilizer treatments were examined for their impact on peppermint growth, essential oil (EO) content, and bioactive properties. Results show that incorporating vermicompost with chemical or bio-fertilizers significantly improves plant height, dry leaf weight, leaf number, and drug fraction by 18.6%, 82%, 27.5%, and 25%, respectively. Vermicompost application leads to a remarkable 100% increase in EO content and a substantial 31.5% rise in menthol percentage compared to other treatments. Furthermore, the study reveals that enhanced menthol production coincides with improved antibacterial and antifungal properties of the plant extract and EO. Treatments with bio-fertilizers demonstrate a significant 23.4% increase in menthol percentage compared to the control group. Vermicompost usage also boosts the total phenolic, flavonoid, and anthocyanin content, along with the antioxidant capacity by 5%–17%. Replacing chemical fertilizers with vermicompost and bio-fertilizers shows notable improvements in peppermint production. These findings underscore the significance of employing organic fertilizers as a means to advance sustainable agricultural practices, mitigate health hazards linked to water contamination, and optimize the cultivation of peppermint as a globally valuable medicinal plant.

History of studying essential oil crops

The article provides a historical overview of the formation and development of the essential oil industry. The main attention is paid to the history of scientific research of essential oil and medicinal plants. Our ancestors may have instinctively selected certain herbs for healing, just as sick animals today seek out plants they wouldn't normally eat. Based on an analysis of the current state, the need has been determined and the priority tasks have been formed for a full-scale revival of essential oil production in the Russian Federation.The problem tatement: in the republics of the former USSR, a number of essential oil plants were cultivated: coriander, lavender, essential oil rose, sage and others. In the 1970-80s the area of cultivation of essential oil plants exceeded 200 thousand hectares. At the same time, the demand for essential oils and other essential oil products was not fully satisfied. With the collapse of the USSR, the essential oil industry declined. The growing areas for most of the essential oil crops ended up on the territory of the newly formed independent states. The cultivation and processing of essential oils in Russia decreased significantly.The goal of the research is substantiation and acquisition of knowledge about the history of emergence and development of essential oil plants, herbal medicine, active substances as a tool for developing a common problem and combining the scientific, industrial and innovative potential of all interested parties.The research methods used are source study, historical-typological and biographical methods. The research results: the opportunity to gain new knowledge about the history of the origin and distribution of essential oil plants, and the creation of a huge food base for animals and people.

Research on the Supercritical CO2 Extraction Process of Hetian Rose Essential Oil

A longstanding concern in plant essential oil extraction is how to optimize extraction efficiency with limited materials. Supercritical CO2 extraction has been proven effective in enhancing the yield and efficiency of extracting plant essential oils. However, the impact of temperature, pressure, and co-solvent content on extracting Hetian rose essential oil remains unclear. There is a lack of research on the influence of pretreatment methods. This study focuses on investigating supercritical CO2 extraction of rose essential oils from Xinjiang Hetian. The research analyzes the effects of pressure and temperature on the extraction rate and validates the efficiency by calculating the solubility of essential oils in supercritical fluid. Under conditions of 35 MPa, 40 °C, 10 L/h, and a particle size of 0.8 mm, this study evaluates the extraction efficiency using Xinjiang Hetian rose materials pretreated with salt solutions at concentrations of 5%, 10%, and 20%, as well as enzyme solutions at concentrations of 2%, 5%, and 10%. Results indicate that appropriate solution concentration can enhance the extraction effect and mass transfer process, but excessively low or high concentrations do not contribute to improved extraction reactions. The highest extraction rate (8.99%) is achieved using a salt solution concentration of 10%, while the lowest (4.21%) is obtained with a salt solution concentration of 20%.