Essential Oil Of Aerial Parts Research Articles

Study on Comparative Chemical Composition and Biological Activities in the Essential Oils from Different Parts of Coleus barbatus (Andrews) Bent. ex G. Don

The aim of the study was to compare the chemical composition and biological activities of Coleus barbatus aerial part essential oil (CBAEO) and Coleus barbatus root part essential oil (CBREO). The hydrodistilled essential oils (EOs) were analysed by gas chromatography-mass spectrometry (GC-MS) and biological activities were investigated in terms of antioxidant, in vitro anti-inflammatory, herbicidal, antibacterial, antifungal and antifeedant properties. GC-MS analysis led to the identification of 45 and 59 components contributing 86.7 % and 85.8 % in CBAEO and CBREO respectively. CBAEO was dominated by oxygenated monoterpenes (31.1 %) while CBREO was dominated by monoterpene hydrocarbons (29.1 %). The EOs exhibited good to moderate antioxidant potential assayed by DPPH radical, hydroxyl radical, nitric oxide radical and superoxide radical scavenging, reducing power and metal chelating activity. CBREO showed good anti-inflammatory activity (76.9 %) than CBAEO (64.7 %). Both the EOs exhibited moderate herbicidal potential against Raphanus raphanistrum subsp. sativus seeds. CBREO was observed to exhibit more antibacterial efficacy than CBAEO against Bacillus cereus and Escherichia coli. Both EOs exhibited significant antifungal activity against Alternaria alternata and Curvularia lunata and a strong antifeedant activity against third instar larvae of Spilosoma obliqua. The results obtained revealed strong to moderate efficacy of CBAEO and CBREO, which might serve as a bioactive source of natural antioxidants and an anti-inflammatory agent. The significant herbicidal, antimicrobial and anti-feedant activities of both the EOs might be helpful for the development of environmentally benign herbicides, fungicides and natural anti-feedant that could be an alternative of synthetic chemicals in future after proper clinical trials.

Post-Harvest Enhancing and Botrytis cinerea Control of Strawberry Fruits Using Low Cost and Eco-Friendly Natural Oils

This work investigates an experimental study for using low-cost and eco-friendly oils to increase the shelf life of strawberry fruit. Three natural oils were used: (i) Eucalyptus camaldulensis var obtuse, (ii) Mentha piperita green aerial parts essential oils (EOs), and (iii) Moringa oleifera seeds n-hexane fixed oil (FO). Furthermore, a mixture of EOs from E. camaldulensis var obtusa and M. piperita (1/1 v/v) was used. The treated fruits were stored at 5 °C and 90% relative humidity (RH) for 18 days. HPLC was used to analyse the changes in phenolic compounds during the storage periods. The effects of biofumigation through a slow-release diffuser of EOs (E. camaldulensis var obtusa and M. piperita), or by coating with M. oleifera FO, were evaluated in terms of control of post-harvest visual and chemical quality of strawberry fruits. The post-harvest resistance of strawberry fruits to Botrytis cinerea fungal infection was also evaluated. As a result, the EO treatments significantly reduced the change in visual and chemical quality of strawberry fruit. Additionally, changes in the titratable acidity of moringa FO-coated strawberry fruits were delayed. EO treatments improved total soluble solids, total phenols, ascorbic acid, antioxidants and peroxidase. E. camaldulensis var obtusa and M. piperita (1/1 v/v) EO-vapour fruit exhibited a slower rate of deterioration, compared to other treatments in all tested, in two experiments. The lowest colour change (ΔE) was observed inthe fruit treated with E. camaldulensis var obtusa EO and M. oleifera FO. HPLC showed changes in phenolic compounds’ concentration, where p-coumaric acid, caffeic acid, gallic acid, ferulic acid and ellagic acid were mostly identified in the fruits treated with the oils. SEM examination confirmed the potential decrease in fungal growth as the fruits were treated with EOs. In conclusion, the treatment of EOs during different storage periods showed promising characterisations for strawberry fruit quality.

In Vitro Antifungal Activity and Chemical Composition of Piper auritum Kunth Essential Oil against Fusarium oxysporum and Fusarium equiseti

The essential oils of plants of the genus Piper have secondary metabolites that have antimicrobial activity related to their chemical composition. The objective of our work was to determine the chemical composition and evaluate the antifungal activity of the aerial part essential oil of P. auritum obtained by hydrodistillation on Fusarium oxysporum and Fusarium equiseti isolated from Capsicum chinense. The antifungal activity was evaluated by direct contact and poisoned food tests, and the minimum inhibitory concentration (MIC50) and maximum radial growth inhibition (MGI) were determined. The identification of oil metabolites was carried out by direct analysis in real time mass spectrometry (DART-MS). By direct contact, the essential oil reached an inhibition of over 40% on Fusarium spp. The 8.4 mg/mL concentration showed the highest inhibition on F. oxysporum (40–60%) and F. equiseti (>50%). The MIC50 was 6 mg/mL for F. oxysporum FCHA-T7 and 9 mg/mL for F. oxysporum FCHJ-T6 and F. equiseti FCHE-T8. DART-MS chemical analysis of the essential oil showed [2M-H]− and [M-H]− adducts of high relative intensity that were mainly attributed to eugenol and thymol/p-cimen-8-ol. The findings found in this study show a fungistatic effect of the essential oil of P. auritum on Fusarium spp.

Chemical composition of Callistemon citrinus (Curtis) Skeels aerial part essential oil and its pharmacological applications, neurodegenerative inhibitory, and genotoxic efficiencies.

Callistemon citrinus aerial part essential oil (CCEO) was analyzed for chemical compositions using GC/MS. Pharmacological activities such as neurodegenerative inhibitory, antioxidant, anti-inflammatory, antimicrobial, and acetylcholinesterase activities were evaluated using the standard methodologies. Genotoxicity was investigated using Allium cepa assay. GC/MS analysis identified 27 compounds; eucalyptol (55.40%) was the major component. Radical scavenging activity showed IC50 value of 16.71μg/mL. Protein denaturation assay showed IC50 value of 21.19μg/mL and 19.53μg/mL in protease inhibitor activity. MIC assay revealed antimicrobial potential of CCEO against microbial strains B. cereus at 2.00mg/mL, S. typhimurium at 4.50mg/mL, S. mutans at 2.50mg/mL, C. albicans at 4.00mg/mL, and S. cerevisiae at 4.50mg/mL concentrations. Mitotic index value (MI) of CCEO showed negligible genotoxicity with MI 17.25% close to distilled water 18.22%. Acetylcholinesterase strong inhibitory activity of CCEO was observed from IC50 =6.335μg/mL. CCEO could be a cheap and easy source for the extraction of the pure compound eucalyptol and possess various biological activities which increase its pharmacological value as well as its applicability in the field of flavor and fragrance industries. PRACTICAL APPLICATIONS: Eucalyptol is the major component of many pharmaceutical and food/flavor industries. The present investigation provides a new source for the isolation of pure compound eucalyptol in cost-effective way. Additionally, the essential oil could also be used for the pharmaceutical formulation of antioxidant, inflammation inhibitory, and neurodegenerative inhibitory drug preparation under the safety range concentration.

Binding of Foeniculum vulgare essential oil and its major compounds to double-stranded DNA: In silico and in vitro studies

Cancer is considered the second leading cause of death in the world. DNA is one of the most important targets in designing anticancer drugs. Changing the gene expression by sequence-specific DNA binders is a rational approach to combat cancer. We evaluated the interaction profiles of aerial part and seed essential oils of Foeniculum vulgare, anethole, and limonene with DNA using in vitro and silico methods. The antioxidant activity of the seed essential oil (IC50 = 5.58 ± 0.53 mg/ml) was higher than the aerial part essential oil (IC50 = 15.6 ± 0.06 mg/ml). The Ka of aerial part essential oil to DNA was more than the seed essential oil. Anethole showed more affinity to DNA compared to d-limonene. Molecular modeling studies showed that anethole formed more stable and stronger interactions with both GC and AT-rich DNA strands than limonene. Anethole demonstrated insignificant selectivity toward the AT-rich DNA strand.

GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg

Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.

Antibacterial activity and essential oil composition of Calendula arvensis L.

Essential oil composition and antibacterial activity of Calendula arvensis L. were investigated. The essential oil of aerial part was obtained through hydro-distillation using a Clevenger type apparatus with 0.38 (v/w) yield. The essential oil components were determined by GC-MS analyses. Thirty-six components were identified in the essential oil that represented 91.8 % of the oil. The major components of the essential oil were δ-cadinene (14.8 %), epi-cubebol (10.7 %), α-cadinol (8.5 %), cubenol (7.7 %), cubebol (7.2 %), 1-epi-cubenol (5.4 %) and ledene (5.1 %). Antibacterial activity of essential oil was observed against Staphylococcus aureus ATCC 29213, Bacillus cereus ATCC 14579, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 by using a broth microdilution. The essential oil showed weak inhibitory activity against E. coli and B. cereus at 8 mg/mL. The oil didn‘t show any antibacterial activity against S. aureus and P. aeruginosa. These results revealed that the oil was rich in oxygenated sesquiterpenes as well as had a weak antimicrobial activity.

Phytochemical Study on the Essential Oils of Tarragon (Аrtemisia dracunculus L.) Growing in Tajikistan and Its Comparison With the Essential Oil of the Species in the Rest of the World

The aerial parts of the tarragon ( Artemisia dracunculus) were collected around Kukteppa village, Ziddi, Varzob region of Tajikistan. The essential oil of tarragon was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. Forty-five compounds representing 99.8% of total oil were identified. Sabinene (29.1%), estragole (24.6%), limonene (7.8%), ( Z)-artemidin (4.9%), myrcene (4.8%), and ( E)-β-ocimene (4.0%) were components with a representation higher than 4% of the essential oils of aerial parts of tarragon. Hierarchical cluster analysis of А. dracunculus essential oils on the global phytogeographic origin based on 30 essential oil components and 105 samples (globally) of this species, indicated the existence of its 7 major chemotypes: ocimene, α-terpinene, capillene, methyl eugenol, mixed chemotype, ( Z)-artemidin, and estragol chemotypes. The essential oils of А. dracunculus showed weak antioxidant and antibacterial activities. To our best knowledge, this is the first report concerning the chemical composition, chemotypic variation, antioxidant, and antimicrobial activities of the essential oils obtained from the aerial parts of А. dracunculus, growing wild in the Varzob region of Tajikistan.

Artemisia biennis Willd.: Anti-Nociceptive effects and possible mechanisms of action

Ethnopharmacological relevance: Artemisia biennis Willd. (Dermane in Persian) has been used as an antinociceptive remedy in Iranian folkloric medicine. ObjectiveThe aim of the present study was to evaluate the anti-nociceptive effects of Artemisia biennis Willd. aerial part essential oil (ABAEO) on male Swiss mice. Materials and methodsNociceptive pain techniques including acetic acid-induced writhing (AAIW), formalin-induced paw licking (FPL), glutamate-induced paw licking (GPL), and tail-flick (TF) models were applied. We assessed opioid and L-arginine-NO-cGMP-KATP pathways to detect the possible anti-nociceptive properties of ABAEO. In addition, neuropathic pain was induced by the cervical spinal cord contusion model. ResultsABAEO (120 mg/kg) had a significant anti-nociceptive activities in comparison to the control animals (p < 0.05) in the AAIW, TF, GPL, and FPL assays. The selective opioid antagonist (naloxonazine) administration in the AAIW test alleviated the anti-nociceptive effect of ABAEO (p < 0.05). L-arginine, methylene blue, and glibenclamide treatment prevented the ABAEO anti-nociceptive effects (p < 0.05); however, sodium nitroprusside could profoundly potentiate the ABAEO-associated antinociception in the FPL (phase II) test (p < 0.05). In nociceptive pain models, Cr (one of the main constituents of ABAEO) showed significant anti-nociceptive effects (p < 0.05). Moreover, the von Frey results indicated that ABAEO could attenuate mechanical allodynia in mice. ConclusionOur observation revealed the anti-nociceptive effects of ABAEO in male mice. These effects could include, at least in part, modulating glutamatergic mechanisms via opioid systems. Our data output also indicates activating the L-arginine-NO-cGMP-KATP system in ABAEO anti-nociceptive activities.

North-East Indian Chromolaena odorata (L. King Robinson) Aerial Part Essential Oil Chemical Composition, Pharmacological Activities - Neurodegenerative Inhibitory and Toxicity Study

Current study was formulated to analyze the chemical composition, pharmacological activities, and toxicology study of Chromolaena odorata aerial part essential oil (COEO) grown in North East India. Gas Chromatography/Mass Spectroscopy was used for chemical composition analysis. The antioxidative potential was tested using DPPH and reducing power assays. Protein denaturation and protease inhibitor assays for anti-inflammatory activity and Allium cepa assay were used to test the genotoxicity of the essential oil. Standard disc diffusion and minimum inhibitory assays (MIC) were employed to test the antimicrobial efficacy of COEO and Acetylcholinesterase (AChE) inhibitory assay was used for anticholinesterase activity. GC/MS analysis revealed Geijerene (26.34 %), α-Copaene (17.87 %), Caryophyllene (11.14 %), 3-Carene (10.63 %), Cadinene (7.98 %), Cyclohexane, 1-Methylene-4-(1-methylethenyl)-(3.37 %), Elemol (2.89 %), τ-Muurolol (2.77 %), as major components. Standard DPPH, protein denaturation, and protease inhibitor assays revealed (IC50= 15.88 μg/mL, 19.35 μg/mL, and 27.28 μg/mL respectively) strong antioxidant, anti-inflammatory potential of COEO. Allium cepa assay showed negative genotoxicity with (mitotic index) MI=16.38 %, which is close to distilled water MI=19.20 %. While disc diffusion assay (zone of inhibition for B.cereus 16.56 ± 0.002 mm; S. aureus 14.20 ± 0.057 mm; S. cerevisiae 10.67 ± 0.015 mm; A. fumigatus 10.15 ± 0.05 mm) and MIC (B. subtilis, S. aureus 2.50 μL/mL), showed the antimicrobial potential of COEO. Strong AChE inhibitory activity was observed with an IC50 value of 61.98 μg/mL. From all the results obtained it is clear that COEO could be used for future research, development, and in preparation of cost-effective pharmacologically important herbal drugs after proper validations.

Essential oil composition of three Iranian Hypericum species collected from different habitat conditions

Abstract The genus Hypericum consists of the most important medicinally active plants with a wide distribution throughout the world. In the present study, 18 populations belong to three Hypericum species (H. asperulum Jaub. & Spach (HA), H. scabrum (HS) and H. vermiculare Boiss. & Hausskn. (HV)) were collected at flowering stage from different habitats. The essential oils of aerial part of plants were extracted by water distillation method and analyzed using GC-FID and GC-MS. The essential oil content of populations varied from 0.02% (in HV3) to 0.43% (in HS4). The highest essential oil content among populations of H. asperulum, H. scabrum and H. vermiculare were belonged to the HA3 (0.4%), HS4 (0.43%) and HV5 (0.38%) populations, respectively. The number, type and percentage of essential oil constituents varied among different populations. Generally, α-pinene, caryophyllene oxide, E-caryophyllene and spathulenol were the dominant components in H. asperulum and H. vermiculare populations; however α-pinene, β-pinene, limonene and E-caryophyllene were identified as the major constituents of H. scabrum populations. The major components of the essential oil (except spathulenol) were not significantly affected by habitat conditions. Cluster analysis based on the essential oil content and essential oil components divided the populations into three distinct groups. Overall, based on the essential oil content, HA3, HS4 and HV5 were identified as the superior populations. Chemical diversity in the essential oil of studied Hypericum populations provides possibility of selection of specific population for different industries.

Chemical composition and antimicrobial activity of essential oil from the aerial parts of Plantago afra L. (Plantaginaceae) growing wild in Tunisia

Medicinal and aromatic plants are garnering an increasing interest in industry, academia and health sciences. In passing from plant production to the bioactive product used by Human, many research studies are involved. The current research aimed to determine the chemical composition and to evaluate the antimicrobial effects of the essential oil extracted from the black psyllium (Plantago afra L.). The aerial parts essential oil obtained by hydrodistillation was analyzed using gas chromatography coupled to mass spectrometry (GC–MS) and flame ionization detection (GC-FID), whereas the microdilution technique was developed to evaluate its antimicrobial efficacy. The main identified constituents were thymol (14.3%), 3-[4-(t-Butyl) phenyl] furan-2,5‑dione (12.7%), palmitic acid (8.9%) and eudesmane (4.7%). The antimicrobial activities were assessed against five standard bacterial strains: Escherichia coli (ATCC 8739), Salmonella enterica (CIP 8039), Pseudomonas aeruginosa (ATCC 9027), Staphylococcus aureus (ATCC 6538) and Bacillus subtilis (ATCC 6633) along with the yeast Candida albicans (ATCC 30031). The results demonstrated that the effective Plantago afra essential oil could be potentially utilised in controlling some phytopathogenic fungi and bacteria.

Chemical Composition, Antioxidant, and Antimicrobial Activities of the Essential Oils From Аrtemisia annua L. Growing Wild in Tajikistan

The aerial parts of Аrtemisia annua L. were collected from Varzob, Rudaki, and Hisor regions of Tajikistan. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry. The essential oils of aerial parts of А. annua were dominated by the monoterpenoids like camphor (32.5%-58.9%), 1,8-cineole (13.7%-17.8%), camphene (4.5%-8.4%), and α-pinene (1.9%-7.3%). Hierarchical cluster analysis of A. annua essential oils indicated the existence of 3 A. annua chemotypes: camphor/1,8-cineole, camphor, and artemisia ketone. The essential oils of А. annua show weak antioxidant activity and average antibacterial activity. In our opinion, the antibacterial activity of А. annua essential oils is related to the presence of 1,8-cineole. To our best knowledge, this is the first report concerning the chemical composition, chemotypic variation, antioxidant, and antimicrobial activities of the essential oils obtained from the aerial parts of А. annua, growing wild in Tajikistan.

Antimicrobial Effect of Essential Oil of Thymus capitatus from Northern Cyprus and Its Gargle Preformulation

Aims: The aim of this study is to prepare herbal gargle preformulations making use of essential oil of aerial parts of Thymus capitatus growing wild in Northern Cyprus and comparing antimicrobial efficacy between these formulations with pure essential oil.&#x0D; Place and Duration of Study: Department of Pharmaceutical Technology, Faculty of Pharmacy, Near East University, Nicosia, TRNC and Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ankara University, Ankara, Turkey, between January 2017 and June 2017.&#x0D; Methodology: Preformulations with three different concentrations of Thymus capitatus essential oil were prepared by using simple preparation method. Preformulation studies were done in lab with less than 500 ml. There is not specific process parameters. Manufacturing process and process parameters will be clarified with further studies. These compositions and the essential oil were tested in-vitro for antimicrobial activity studies by using broth microdilution method. Staphylococcus aureus ATCC 43300, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae RSKK 574, Salmonella paratyphi C, Pseudomonas aeruginosa ATCC 9027, Pseudomonas aeruginosa ATCC 27853 as bacteria and Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019 as yeasts were used for antimicrobial activity tests.&#x0D; Results: All trials were found to be more effective than EO, and a significant effect was observed when compared to the values ​​of standard antimicrobial agents.&#x0D; Conclusion: TheThymus capitatus growing in Northern Cyprus could be used as a herbal raw material, essential oil source in developing herbal gargle preformulations to reduce fungal and bacterial infections in mouth.

Antibacterial activity of Centaurea pumilio L. root and aerial part extracts against some multidrug resistant bacteria

BackgroundIn the context of searching for potent, safe, natural antimicrobial agents to combate the global antimicrobial resistance (AMR) phenomenon, the current study evaluates for the first time ever, the broad-spectrum antimicrobial activity of essential oil (EO) and extracts from the rare wild plant Centaurea pumilio L.. It has tremendous ethnomedicinal values; its dried root is used as a fattening agent, a treatment for bad breath and diabetes, and screened for schistosomicidal activity.MethodsC. pumilio EO was extracted by hydrodistillation using a Clevenger apparatus. Chemical constituents of aerial part were extracted using a sequential solvent/solvent procedure employing four solvents with increasing polarities in the following order: petroleum ether, chloroform, ethyl acetate, and n-butanol. The chemical constituents were identified by GC-MS. Fifty-two microbial strains were used; twenty-six multidrug resistant (MDR), sixteen clinical, and ten reference strains. The identification of the microbial strains was performed by MALDI-TOF-MS. The antimicrobial activity of the EO and the aerial part and the root extracts was assessed through disc diffusion assay. A minimum inhibitory concentration (MIC) of the EO and extracts was determined using the broth micro-dilution method.ResultsThe growth of reference and clinical strains was inhibited by EO, methanol, chloroform, and ethyl acetate aerial part extracts and chloroform root extract. The MDR strains growth, however, was inhibited only by EO and chloroform aerial part extract. GC-MS identified for the first time eighteen constituents from aerial part EO and chloroform extract each. EO showed antimicrobial activity against the reference, clinical, and MDR strains with MIC values of 31.25–125, 31.25–125, and 62.50–250 μg/mL, respectively. Methanol aerial part extract exhibited high antimicrobial activities with MIC values of 62.50–250 μg/mL against reference and clinical strains. Chloroform root extract displayed strong antimicrobial activity against reference and clinical strains recording MIC values of 62.50–250 μg/mL and 62.50–125 μg/mL, respectively. The chloroform aerial part extract demonstrated potent antimicrobial activity against the reference, clinical, and MDR strains with 31.25, 31.25, and 15.62 μg/mL MIC values, respectively.ConclusionsPresent data unravel the C. pumilio pharmacological magnitude to discover eco-friendly potent antimicrobial agents to fight AMR phenomenon.

In vitro antioxidant activity of essential oil of aerial parts of Mentha pulegium L

This work was conducted to evaluate the antioxidant activity of the essential oil obtained from the aerial part of Mentha pulegium. The antioxidant power of the essential oil obtained by hydrodistillation using a Clevenger-type apparatus was evaluated by using three methods: free radical-scavenging activity, reducing power and liver lipid peroxidation assay. Results showed that Mentha pulegium oil displayed good quality according to its physicochemical characteristics, and a higher yield 5.1 ± 0.2%. The essential oil of Mentha pulegium showed a higher DPPH radical scavenging activity 90.54 ± 1.5 % at a concentration of 1000 µg/mL. This value was close to the results obtained with ascorbic acid 96.23 ± 1.2%, and catechin 94.50 ± 1.4%. This oil exhibited significant potential for reducing iron (the value observed by optical density was 1.8 ± 0.3), while ascorbic acid and catechin provided an OD of 2.069 ± 0.03 and 2.66 ± 0.016 at the same concentration of 1000 µg/mL. The tested oil protected against lipid peroxidation induced by Fe+2, and considerably increased the percentage of anti lipid peroxidation in a dose-dependent manner. The studied oil displayed a good degree of antioxidant activity and can be exploited in food and pharmaceutical industries.

Chemical constituents of Artemisia nilagirica (Clarke) from Western Himalaya of Uttrakhand, India

Chemical composition of the essential oil of aerial parts of Artemisia nilagirica (Clarke) of western Himalaya were analyzed by GC-FID and GC-MS. The chemical constituents of essential oil composition of aerial parts of Artemisia nilagirica was dominated by artemisia ketone (55.07%), s-Caryophyllene (6.5%), Germacrene D (5.8%), Perillene (3.2%).

Identification of non-alkaloid natural compounds of Angelica purpurascens (Avé-Lall.) Gilli. (Apiaceae) with cholinesterase and carbonic anhydrase inhibition potential

In current study is done antioxidant, anticholinesterase, and carbonic anhydrase isoenzymes I and II inhibition assays, screening of biological active compounds and electronic microscopy analysis of secretory canals of fruits, flowers, roots, and aerial parts extracts and essential oils of Angelica purpurascens. Phenolic constituents, antioxidant, and anti-lipid peroxidation potentials of variants were estimated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and thiobarbituric acid (TBA) processes. Cholinesterase inhibition effect was detected through Ellman’s method. The GC/ Mass Spectrometry (MS) and gas chromatography (GC)-flame Ionization Detector (FID) was used for essential oils analysis. NMR techniques was used for identification of the isolated compounds. The fruit hexane and dichloromethane fractions exhibited a greater antioxidant capacity and total phenolic content. The dichloromethane fraction of fruit demonstrated the most higher acetylcholinesterase inhibition (39.86 ± 2.63%), while the fruit hexane fraction displayed the best inhibition towards butyrylcholinesterase (84.02 ± 1.28%). Cytosolic isoenzymes of human carbonic anhydrase (hCA) I, and II isoenzymes were influentially suppressed by flower and fruit dichloromethane fractions with 1.650 and 2.020 µM IC50 values, respectively. The electronic microscopy analysis of secretory canals found that the small number of secretory canals were at leaf while the largest shape of secretory canals was at the fruit. The secretory canals of roots, aerial parts, and fruits include more monoterpene hydrocarbons, while the canals, existing in the flowers are qualified by a higher presence of sesquiterpenes β-caryophyllene (12.1%), germacrene D (4.5%) and ether octyl acetate (11.9%). The highest level of monoterpene β-phellandrene (47.6%) and limonene (8.2%) were found in the fruit essential oil. The next isolated compounds from fruits of A. purpurascens like stigmasterol, β-sitosterol, bergapten, and oxypeucedanin have shown high anticholinesterase and antioxidant activities.

Essential Oil Composition of Salvia candidissima Vahl. occidentalis Hedge, S. tomentosa Miller and S. heldreichiana Boiss. Ex Bentham from Turkey

Previous reports clearly indicate that different chemotypes of Salvia heldreichiana, S. tomentosa and S. candidissima are present. In current study we have investigated essential oil composition of Salvia candidissima ssp. occidentalis, Salvia heldreichiana from Konya and Salvia tomentosa from Eskisehir, Turkey. In GC/MS analysis 89.30 ± 1.034 % (n=3) of the S. heldreichiana oil was identified which corresponds to 83 compounds. Main components of the oil were α-pinene 9.57 ± 0.406 %, borneol 9.21 ± 0.193 % and caryophyllene oxide 7.58 ± 0.052 %. Seventy-seven compounds were identified in aerial parts essential oil of S. candidissima ssp. occidentalis that sums up to 62.00 ± 0.962 % (n=3) of the oil. Main components were hexadecanoic acid 4.73 ± 0.022 %, m-cymene 4.00 ± 0.152 % and manool oxide 3.65 ± 0.057 %. One hundred-four compounds were identified in the aerial parts essential oil of S. tomentosa which corresponds to 89.91 ± 0.444% (n=3). The main components were, α-pinene 15.25 ± 0.621 % and borneol 7.88 ± 0.273 %. Present study revealed α-pinene/borneol/caryophyllene oxide/terpinen-4-ol chemotype of S. heldreichiana, α-pinene/borneol chemotype of S. tomentosa as well as chemical composition of S. candidissima ssp. occidentalis essential oil for the first time.

Toxic essential oils, part VI: Acute oral toxicity of lemon balm (Melissa officinalis L.) essential oil in BALB/c mice

Despite being renowned for its volatiles, the data on the toxicity of the essential oil of lemon balm (Melissa officinalis L., Lamiaceae) is rather limited compared to its solvent/water-soluble extractibles. In this study, the aerial parts essential oil of M. officinalis, with over 130 constituents identified herein, 26 of which detected for the first time, was investigated for acute oral toxicity in BALB/c mice. The oil, composed of predominantly monoterpene aldehydes, citronellal (21.2–21.8%), neral (17.8–18.4%), and geranial (22.9–23.5%), which were assayed in parallel with the oil in some tests, induced significant changes in animal behavior, as well as altered biochemical parameters reflecting liver and kidney functions. Different pathological changes in the stomach, duodenum, liver, and kidneys were detected when the oil was administered in doses higher than 1 g kg−1. A depletion in the liver/kidney antioxidant capacities and an increased rate of lipid peroxidation was noted for animals treated with lemon balm oil. The calculated value of the oral LD50 in BALB/c mice (2.57 g kg−1) infers that the essential oil is only moderately toxic.