Essential Oil Fraction Research Articles

DESCRIPTION OF A COLLECTION OF SALVIA SCLAREA L. IN TERMS OF ITS BIOCHEMICAL PROPERTIES

The study object is the accumulation of essential oil in the inflorescences of clarysage, the content of its main components and the sclareol yield. The study purpose is to analyze a collection of clarysage (112 specimens) in terms of the basic biochemical properties (the essential oil content in raw material, its chemical composition and the sclareol yield) and to identify the specimens showing good prospects for inclusion in the breeding process. The study was carried out in 2018–2020 at the experimental plot of the Research Institute of Agriculture of Crimea located in the piedmont region of Crimea (KrymskayaRoza village, Belogorsk district). The climate is moderate continental. The average year-round temperature is +10 °С. The average year-round air humidity is 70%, the hydrothermic factor is 0.91. The accounting and analyses were performed in accordance with the techniques developed. The study revealed higher degree of genetic determination of the essential oil buildup in plants as compared to the weather effect. As noted, increased precipitation and extremely high temperatures during the plant flowering and seed formation time affect negatively the sclareol buildup. The variability of the properties analyzed (Cv=7.8–23.4%) bears evidence to a feasibility of selecting promising parent material for breeding. Based on the outcomes of the collection analysis there have been isolated 12 specimens leaving behind the varieties cultivated in terms of the basic biochemical properties including five specimens with a high mass fraction of essential oil – 0.90–1.06% on the dry basis (as compared to 0.6–0.88% in the varieties cultivated) and seven specimens with a high sclareol content in raw material – 2.00–2.35% (as compared to 1.58–1.71% in the varieties cultivated).

Obtaining thymoquinone and thymohydroquinone from Wild bergamot (Monarda fistulosa L.)

Background: Thymoquinone (TQ) and thymohydroquinone (THQ) are natural substances with antibacterial, antimycotic, antiviral, anticancer, antioxidant activity. Previously, was developed the method for obtaining Wild bergamot essential oil with high TQ content. The objective of this study was to create methods for obtaining individual substances TQ and THQ from Wild bergamot herb. Material and methods: Aerial parts of Wild bergamot have been collected from the plantation of the Scientific Practical Centre of Nicolae Testemitanu State University of Medicine and Pharmacy. The hydrodistillation method has been used for essential oil isolation and fractionation, and purification of individual substances. The analytical part of study has been performed by the HPLC method. Results: THQ was obtained in crystalline form by reducing TQ directly in the essential oil with ascorbic acid, sulphurous acid, or natural components of the Wild bergamot essential oil. Subsequent oxidation of THQ with hydrogen peroxide or nitrous acid gave TQ. Additionally, the yield of TQ was increased by optimising the parameters of plant material fermentation and using two-stage hydrodistillation with intermediate oxidation of the THQ formed at the first stage. Conclusions: Several technological procedures were created to obtain THQ from the Wild bergamot essential oil with a yield from 68-74% to 93-97% and TQ from THQ with a yield of 87-92%. The procedure of plant material processing has been optimised also to obtain essential oil with 48-56% TQ and not more than 12% residual phenols.

Изучение коллекции розы эфиромасличной по комплексу хозяйственно ценных показателей

Abstract. The article presents the results of the study of essential oil-bearing rose samples according to the most valuable indicators. The purpose of the current research was twofold: characterize essential-oil-bearing rose collection in terms of the morphobiological parameters and economically valuable traits; identify samples promising for selection. Research methods. Analysis of essential-oil-bearing rose samples was carried out according to the methodological guidelines for essential oil crops in 2017–2020. Under field and laboratory conditions, we analyzed such indicators as flowering time, winter hardiness, budding ability, flower weight, essential oil content in inflorescences. Statistical processing of the data obtained was carried out. Results. The essential-oil-bearing rose collection nursery was laid in 2012 at the experimental site of the Department of Aromatic and Medicinal Plants located in the foothill zone of Crimea (Krymskaya Roza vill., Belogorskiy district). Significant variability of the flower weight and mass fraction of essential oil (Cv = 24.6…36.3 %) was noted. The dependence of the main indicators on meteorological conditions during the period of active vegetation of plants was analyzed. It was found that extremely high temperature regime and insufficient rainfall in April–May reduced the duration of flowering in the essential-oil-bearing rose collection, on average, by 3–5 days. It was noted that, despite the differences in the response of samples to weather conditions, the leading factor determining the level of essential oil accumulation in inflorescences is genotype. According to the complex or individual valuable indicators, we identified 14 best specimens, seven of which were with the highest content of essential oil in inflorescences (0.030–0.049 %). Scientific novelty. For the first time, a one-time detailed study of the collection was conducted according to a set of valuable traits. The collection comprises 50 specimens including five varieties of Crimean breeding (originator – Research Institute of Agriculture of Crimea) and five – from Bulgaria. The information obtained will allow selecting samples that are promising as source material for breeding, as well as predicting the expected productivity of essential-oil-bearing rose varieties under specific weather conditions of the region.

Phytochemical Analysis and Evaluation of Antioxidant and Antibacterial Activities of Kratom-Containing Formulations of Ya-Gae-Bid-Na-Ron

Objective: This study aimed to evaluate the antioxidant and antibacterial activities of Ya-Gae-Bid-Na-Ron (YGBNR).Material and Methods: YGBNR formulations were prepared using kratom collected from different provinces in Thailand. Mitragynine content was determined using high-performance liquid chromatography (HPLC), while total phenolic content (TPC), total tannin content (TTC), and total flavonoid content (TFC) were determined using colorimetric methods. Antioxidant power was estimated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP) assays. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of YGBNR were determined using a broth dilution technique and plating of the bacterial solution, respectively.Results: Mitragynine content, TPC, TTC, and TFC varied among different sources of kratom. DPPH assay indicated that YGBNR had an average IC50 of 0.44±0.04 mg/mL, and the FRAP assay ranged from 61.48±0.55 to 102.98±0.94 mg gallic acid equivalent (GAE), per gram of dry extract. YGBNR extracts did not exhibit any antibacterial activity. However, the volatile oil fraction showed MIC against Escherichia coli and Pseudomonas aeruginosa at a concentration of 3 mg/mL.Conclusion: Phytochemical analysis revealed the abundance of phenolic compounds in YGBNR. YGBNR exhibits promising antioxidant potential, but does not exert any antibacterial activity.

Efficacy of green solvents in pungent, aroma, and color extractions of spice oleoresins and impact on phytochemical and antioxidant capacities

The current study investigated the effect of green solvents (ethanol, acetone, and their mixture) on oleoresin yield, extraction of pungent compounds, volatiles, colorants, and the correlations with phytochemical and antioxidant activities of 5 spices (chili, black pepper, turmeric, ginger, and coriander). The solvents’ polarities and boiling points determined the oleoresin extractability, and the solvent mixture recorded the maximum yield in all spices. A total of 33 volatiles and 8 non-volatile compounds were identified in the essential oil and oleoresin fractions. Curcumin and 6-gingerol contributed to strong antioxidant properties with Pearson correlations of 0.999 and 0.924, respectively. Acetone reported the highest yield of curcumin (244.09 ± 1.71 mg/g) and 6-gingerol (131.98 ± 2.86 mg/g), whereas ethanol was the most effective solvent for capsaicinoids and piperine. Chili oleoresin extracted by ethanol delivered the maximum pungency. Acetone displayed the highest extractability of linalool detected in coriander oleoresin and essential oil. Chili oleoresin contained the highest carotenoid yield, and acetone extracted the maximum red and yellow fractions. Chili and black pepper possessed high Browning Indices and positively correlated with the total flavonoid content. β-caryophyllene dominated the essential oil profile of chili and black pepper, and the solvent mixture reported the maximum yields as 47.43% ± 0.69% and 75.11% ± 0.64%, respectively. Conclusively, the solvent mixture extracted the maximum oleoresin yield; acetone showed the highest affinity for colorants, volatiles, and antioxidants in turmeric, ginger, and coriander, while ethanol was the most efficient solvent in extracting bioactive compounds of chili and black pepper.

Essential Oils from Selected Mediterranean Aromatic Plants-Characterization and Biological Activity as Aphid Biopesticides.

The need for alternatives to synthetic pesticides is a priority today, especially when these pesticides are directed against aphids, one of the more challenging pests facing modern agriculture. Essential oils may be one of these alternatives. We assayed the insecticidal potential of essential oils from Thymus vulgaris, Rosmarinus officinalis var. 'prostratus' and Lavandula dentata. Essential oil extraction was carried out by hydrodistillation in a Clevenger-type apparatus for 3 h and their respective composition was elucidated by gas chromatography-mass spectrometry. The essential oil fraction from T. vulgaris contained 81.20% monoterpenoids and 12.85% sesquiterpenoids; R. officinalis var. 'prostratus' contained 91.98% monoterpenoids and 1.93% sesquiterpenoids, while L. dentata contained 69.60% monoterpenoids and 8.05% sesquiterpenoids. The major components found were 1,8-cineole (18.11%), camphor (11.18) and borneol (10.32%) in T. vulgaris; α-pinene (18.72%), verbenone (13.42%) and 1,8-cineole (10.32%) in R. officinalis; and 1,8-cineole (34.65%), camphor (7.58%) and β-pinene (6.39%) in L. dentata. The insecticidal activity of the essential oils was evaluated by contact toxicity bioassays against the bird cherry oat aphid, Rhopalosiphum padi L. We observed a mortality rate of 78.3% ± 23.9 at 15 μL/mL with T. vulgaris, 54.7% ± 25.8 with L. dentata (although at a lower concentration, 10 μL/mL), and 56.7% ± 25.6 at 15 μL/mL with R. officinalis. Our results suggest that thyme essential oil may be particularly promising for integrated aphid management provided that specific conditions of use and dosages are observed.

Quantification of coumarins, furocoumarins and polymethoxyflavones in hydroalcoholic fragrances by supercritical fluid chromatography‐tandem mass spectrometry

ABSTRACT Citrus essential oils, thanks to their pleasant aroma, are certainly the most used ingredients in the formulation of hydroalcoholic fragrances. The non-volatile fraction of Citrus essential oil is composed for 10–20% of coumarins, furocoumarins and polymethoxyflavones. It is well known that furocoumarins induce photosensitization and have potential carcinogenic and mutagenic effects. It follows that furocoumarins levels in cosmetics product are constantly monitored by opinions and regulations issued by the International Fragrance Association. The aim of this research article was to quantify coumarins, furocoumarins and polymethoxyflavones in thirty commercial hydroalcoholic fragrances using supercritical fluid chromatography in combination with triple quadrupole mass spectrometry technique (SFC-QqQ-MS). According to author’s knowledge, this is the first report on the determination of oxygen heterocyclic compounds in hydroalcoholic fragrances by means of SFC-QqQ-MS technique.

New Reports on the Portuguese Endemic Species, Santolina impressa: Secretory Structures, Essential Oil Composition and Antiviral Activity.

Santolina impressa is an aromatic Asteraceae species endemic to Portugal, traditionally used for its anti-inflammatory properties. The aim of this study was to characterize S. impressa secretory structures, analyze the essential oil (EO) from the aerial organs, and evaluate its antiviral activity against herpes simplex viruses HSV-1 and HSV-2. Secretory structures were investigated by light and scanning microscopy, and the secretion was histochemically characterized. The EO from the aerial organs in full blooming was analyzed by gas chromatography with flame ionization detection and gas chromatography-mass spectrometry. Antiviral assays were performed by direct contact with viral suspensions (virucidal effect), and in infected Vero E6 cells, at different time periods during the viral replication cycle. Two types of secretory structures were described, biseriate glandular trichomes and secretory ducts, producing an oleoresin and a resin rich in flavonoids, respectively. Fifty compounds were identified in S. impressa EO, accounting for 87% of the total constituents. Monoterpenes constituted the main EO fraction (82%), with β-pinene (13%) and β-phellandrene (10%) being their major components. The EO interacted with HSV-1 and HSV-2 in a dose-dependent manner, thereby inactivating both viral infections. The EO did not evidence a virucidal effect but inhibited the HSV-1 and HSV-2 infection in Vero cells in a dose-dependent manner. However, further studies are needed to investigate the mode of action in the replication cycle.

Cannabis sativa leaf essential oil fractions and bioactive compounds: chemistry, functionality and health-enhancing traits

Cannabis sativa leaf essential oil fractions and bioactive compounds: chemistry, functionality and health-enhancing traits

Inhibitory effect of Greek oregano extracts, fractions and essential oil on economically important plant pathogens on soybean

Abstract. The inhibitory potential of Greek oregano extracts, fractions and essential oil on three economically important plant pathogens on soybean was examined. In vitro experiments were conducted to determine the effects of essential oil (EO), acetone exudate (AE), methanol extract (ME), and its polar (PF) and non-polar (NPF) fractions, originating from O. vulgare ssp. hirtum plants, on the mycelial growth of Phytophthora sojae, Pythium debarianum, and Alternaria alternata, which were isolated from diseased soybean plants. Seven variants: EO, AE, ME, PF, NPF at concentrations of 100 mg/ml, blank (with the solvent – DMSO) and control (without treatment) were performed with all isolates. The mycelial growth of P. sojae was inhibited to the greatest extent – over 95% reduction of growth under the impact of EO, ME, AE and NPF. In A. alternata variant there was also a high degree of growth inhibition – most pronounced in EO variant (97%) and least in ME variant (78%). The weakest inhibitory effect of the studied extracts was observed in P. debarianum – between 27% (NPF) and 36% (ME). In polar fraction treatments, growth enhancement was observed in P. sojae and A. alternata. Oregano essential oil, methanol extract, acetone exudate and non-polar fraction can be considered as promising candidate active compounds of potential biopesticide formulations for the control of soybean diseases.

Chemical composition, antifungal properties and seed treatment potential of essential oil fractions of Callistemon citrinus against two seed-borne fungi of rice: Alternaria padwickii and Bipolaris oryzae

Chemical composition, antifungal properties and seed treatment potential of essential oil fractions of Callistemon citrinus against two seed-borne fungi of rice: Alternaria padwickii and Bipolaris oryzae

Evaluation of Ionic Liquids for the Sustainable Fractionation of Essential Oils

Evaluation of Ionic Liquids for the Sustainable Fractionation of Essential Oils

Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes

Ethnopharmacological relevanceLippia lacunosa Mart. & Schauer is an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil. It is known as “chá de pedestre” and “rosmaninho” in folk medicine. This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks. It is often confused with and, therefore, used interchangeably with L. rotundifolia and L. pseudothea. Aim of the studyThis study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice. Materials and methodsThe chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC). Carrageenan-induced paw edema was used to investigate the anti-inflammatory activity in mice. Mechanical allodynia induced by carrageenan and hot plate tests were employed to evaluate the antinociceptive activity. ResultsThe main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%). The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone. In experimental models of paw edema and mechanical allodynia induced by carrageenan (600 μg, 30 μL, i.pl.), the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema. The ethanolic extract (100 mg/kg) reduced mechanical allodynia only in the 2 nd h of evaluation. On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period. The hexane extract, essential oil, and majority fraction F33 also reduced the heat-induced nociceptive response. Also, majority fraction F33 did not affect the time mice spent in the rota-rod apparatus. ConclusionsThe elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.

Development of image similarity strategy based on targeted filtration for non-targeted HS-SPME/GC × GC fingerprints of volatile oils from Chinese patent medicines: A case of Chaihu Shugan Wan

To evaluate Chinese patent medicines more comprehensively, image similarity strategy based on targeted filtration was developed for non-targeted two-dimensional (2D) chromatographic fingerprints of volatile oil fraction in this paper. At first, volatiles and semi-volatiles were enriched from Chaihu Shugan Wan (CSW) by head space- solid phase micro-extraction (HS-SPME), and analyzed by comprehensive two-dimensional gas chromatography (HS-SPME-GC × GC). In succession, the inter-group and intra-group differences were distinguished by multi-component spectral correlative chromatography (MSCC). Facing the challenge of data complexity, some 50 contours were selected to indicate various medicinal materials through zone- specific ion filtration in 2D fingerprints. Among them, the known compounds were proven to be effective in “soothing liver-qi stagnation” through PubChem and other bioinformatic tools. Thirdly, Speeded Up Robust Features- Fast Library for Approximate Nearest Neighbors (SURF-FLANN) algorithm was utilized to estimate the pairwise similarities for the simplified fingerprints. In this procedure, the methodological examination was further made through the simulated images, including comparison with other algorithms, consideration for image complexity, and calculation of positives / negatives. At last, the similarities of six sample fingerprints were scored successfully. In summary, image similarity strategy based on zone- specific ion filtration can provide a viable solution for rapidly evaluating 2D fingerprints of extract fractions from Chinese patent medicines.

Enhanced Bioactivity of Rosemary, Sage, Lavender, and Chamomile Essential Oils by Fractionation, Combination, and Emulsification.

This study aimed to increase the bioactivity of essential oils by fractionation, combination, and emulsification. In this regard, pharmaceutical quality Rosmarinus officinalis L. (rosemary), Salvia sclarea L. (clary sage), Lavandula latifolia Medik. (spike lavender), and Matricaria chamomilla L. (chamomile) essential oils were fractionated by vacuum-column chromatography. The main components of the essential oils were verified, and their fractions were characterized by thin layer chromatography, gas chromatography-flame ionization detector, and gas chromatography/mass spectrometry. Besides, oil-in-water (O/W) emulsions of essential oils and diethyl ether fractions were obtained by the self-emulsification method, followed by droplet size, polydispersity index, and zeta potential value measurements. The in vitro antibacterial effects of the emulsions and binary combinations (10:90, 20:80, 30:70, 40:60, 50:50, 60:40, 70:30, 80:20, 90:10, v:v) against Staphylococcus aureus were determined by microdilution. In addition, the in vitro anti-biofilm, antioxidant, and anti-inflammatory effects of emulsion formulations were evaluated. According to the experimental results, fractionation and emulsification enhanced essential oil in vitro antibacterial, anti-inflammatory, and antioxidant effects due to increased solubility and nano-sized droplets. Among 22 different emulsion combinations, 1584 test concentrations resulted in 21 cases of synergistic effects. The mechanism of the increase in biological activities was hypothesized to be higher solubility and stability of the essential oil fractions. Food and pharmaceutical industries may benefit from the procedure proposed in this study.

Extraction and fractionation of long pepper essential oil: process modeling and simulation

Extraction and fractionation of long pepper essential oil: process modeling and simulation

Potential of Tetradenia riparia leaf essential oil and its fractions in controlling Aedes aegypti and Rhipicephalus microplus larvae

Tetradenia riparia (Hochst.) Codd (Lamiaceae) is a shrub, commonly known as ginger bush or false myrrh, and several studies have shown that T. riparia exhibits a variety of biological properties. This study aimed to determine the chemical composition of T. riparia essential oil and its fractions, investigate their anticholinesterase activity, and assess their larvicidal activity against the cattle tick Rhipicephalus microplus and the mosquito Aedes aegypti. Eleven essential oil fractions were obtained by fractionation and analyzed by gas chromatography/mass spectrometry. Larvicidal activity against R. microplus and third-instar A. aegypti was assessed using a larval packet test and a larval immersion test, respectively. Anticholinesterase activity was determined by a bioautographic method. Forty-nine compounds were identified in the essential oil, of which the major classes were oxygenated sesquiterpenes (45.95%) and sesquiterpene hydrocarbons (35.20%) and the major components were isospathulenol (17.40%), β-caryophyllene (15.61%), 14-hydroxy-9-epi-caryophyllene (10.07%), 14-hydroxy-α-muurolene (8.32%), and 9β,13β-epoxy-7-abietene (5.53%). Bioassays showed that T. riparia essential oil (LC50 = 1.56 µg/mL) and FR3 (LC50 = 0.30 µg/mL) were the most active against R. microplus and A. aegypti larvae, respectively. The essential oil and FR1, FR2, and FR3 exhibited acetylcholinesterase inhibitory activity. These results indicate that T. riparia essential oil and its fractions hold promise in the development of novel, environmentally safe agents for the control of R. microplus and A. aegypti larvae.

Anti-inflammatory properties of an aldehydes-enriched fraction of grapefruit essential oil.

Chronic inflammation is linked to the development of numerous diseases and is accompanied by increased cytokine secretion. Macrophages provide a first line of defense against pathogens that under inflammatory stimuli release pro-inflammatory cytokines. The essential oil (EO) fractions obtained from Citrus spp. rich in different compounds have gained the attention of both researchers and users during the last decades. In particular, grapefruit (Citrus paradisi) peel is rich in phenolics and flavonoids with several health benefits, including anti-inflammatory actions. Additionally, its EO consists of a large number of compounds such as monoterpenes, sesquiterpenes, alcohols, aldehydes, esters, and oxides. Among the methods for encapsulating EOs, spray-drying is the main one. In the present study, we aimed to determine the in vitro anti-inflammatory activity of EO from C. paradisi (grapefruit essential oil [GEO]) (whole and fractions) in a lipopolysaccharide (LPS)-induced inflammation model. Results indicate that Fr-GEO and Fr-GEO_SD exert protective effects against LPS-induced inflammation by decreasing gene expression and levels of pro-inflammatory cytokines as IL-6 and TNF-α. Monoterpenes as the most common components, as well as aldehydes and sesquiterpenes, might be responsible for such effects, although a synergistic action is not excluded. Furthermore, a higher percent of aldehydes is linked to improved olfactory properties. Our findings support the anti-inflammatory effects of selected Fr-GEO with a great potential for the development of new nutraceuticals and/or functional food for the treatment of inflammatory-associated diseases. PRACTICAL APPLICATION: The findings of this study support the anti-inflammatory effects of selected Fr-GEO with a great potential for the development of new nutraceuticals and/or functional food for the treatment of inflammatory-associated diseases.

Chemical profile, antiproliferative and antibacterial activities and docking studies of essential oil and hexane fraction of hydrosol from fresh leaf of Plectranthus amboinicus (Lour.) Spreng.

Essential oils are commonly extracted from plants by hydrodistillation in which an aqueous phase called hydrosol is obtained. Like essential oil, this by-product of distillation can be a source of natural bioactive molecules with health benefits. This study aimed at the investigation of the chemical profile, antiproliferative and antibacterial activities of essential oil and hexane fraction of hydrosol from Plectrunthus amboinicus (family Lamiacease) fresh leaf cultivated in Sudan. Essential oil was obtained by hydrodistilation and hexane fraction (HF) was derived from hydrosol by liquid –liquid extraction. Results showed that the phenolic monoterpene thymol (72.9%) was the dominant component of the essential oil while the HF was characterized by the presence of long-chain (n-C29 to n-C68) alkanes (91.4%). The HF showed highest antiproliferative activity against the colon cancer HT29 (IC50 9.2 μg/mL) and HCT116 (IC50 7.7 μg/mL) cell lines. The essential oil exerted best antibacterial activity with highest inhibition (22.0–22.3 mm) and minimum inhibitory concentration (31 μg/mL) against the Gram positive bacteria Bacillus subtilis and Staphylococcus aureus. In silico study showed that the major components of the oil displayed higher docking scores than those present in the HF suggesting an antagonistic effect by other molecules in the essential oil. In conclusion, beside the essential oil, the hydrosol possesses promising bioactive composition suggesting that it could be exploited for further therapeutic applications.

Evaluation of anti-oxidant and antimelanogenic effects of the essential oil and extracts of Rosa × damascena in B16F10 murine melanoma cell line.

Rosa × damascena Herrm. belonging to the Rosaceae family has demonstrated anti-inflammatory and anti-oxidant effects previously. Excessive production of free radicals and activation of tyrosinase enzyme caused by UV induces excessive concentration of melanin pigment and skin spots in the long term. Therefore, finding natural sources with anti-oxidant and antityrosinase effects helps to regulate the melanogenesis process. In the current research, we investigated the antimelanogenic, anti-oxidant, and anti-tyrosinase effects of its essential oil, methanol extract (MeOH), and different fractions including n-hexane, dichloromethane (CH2Cl2), n-butanol (BuOH), ethyl acetate (EtOAc), and H2O of R. × damascena in B16F10 cell line. For this purpose, impacts of extracts and essential oil of R. × damascena were investigated on cell viability, cellular tyrosinase, melanin content, mushroom tyrosinase, reactive oxygen species (ROS) production, as well as the amount of tyrosinase protein in the B16F10 murine melanoma cell line. Essential oil, MeOH, and different fractions of R. × damascena were not cytotoxic on B16F10 cells. However, they had significant reducing effects on mushroom tyrosinase activity, melanin content, and ROS production. Also, there is a significant decrease in tyrosinase protein levels at 200 µg/ml but not at other concentrations. Therefore, the essential oil, MeOH, and different fractions of R. × damascena had promising antimelanogenic activity via repression of mushroom tyrosinase activity and ROS production.