Essential Fatty Acid Content Research Articles

Elaeagnus umbellata fruit - chemical composition, bioactive compounds, and kinetic of DPPH inhibition compared to standard antioxidants


 umbellata (Autumn Olive) is a medicinal plant used in traditional Asian medicine. In this study, we examine E. umbellata, which grows in central Europe (Poland). Despite significant climatic differences in both regions, we show that the fruits have a similar chemical composition and antioxidant activity to those observed in their native areas. For the first time, we examined the fatty acid composition of E. umbellata, showing that unsaturated fatty content for as much as 88.67% of total fat. Including the content of essential polyunsaturated fatty acids was over 50% of the total fat. We also examined proximate content, the content of fat-soluble vitamins, lycopene, beta carotene, and the mineral composition of the fruit. Moreover, using FT-IR spectrometry, we have shown that the fruits of the Autumn olive can change the distribution of chemical bonds when treated with hydrogen peroxide. The fruits of E. umbellata showed dose-dependent antioxidant properties with IC50 values of 76.27 µg / ml We also investigated the kinetic antioxidant activity of E. umbellata fruits based on DPPH radical inhibition compared to standard antioxidants (vitamin c and BHT). We have shown that using available protocols to spectrophotometrically study antioxidants' reaction with the DPPH radical may underestimate the effects of the Autumn Olive fruits.

Interactive effects of dietary lipid and nutritional emulsifier supplementation on growth, chemical composition, immune response and lipid metabolism of juvenile Nile tilapia (Oreochromis niloticus)

The objective of this study was to investigate the interactive effects of supplementation with different levels of dietary lipids and a commercial nutritional emulsifier (NE) on the growth, feed utilization, immune response and lipid metabolism of juvenile Nile tilapia (Oreochromis niloticus). A 5 × 2 factorial design was formulated with five lipid supplement levels (0, 15, 30, 45 and 60 g/kg) and NE supplementation (with or without NE at 0.35 g/kg). Nile tilapia with an initial average body weight of 8.06 g were randomly placed in 30 cages and offered one of the ten diets for 8 weeks. The results show that dietary NE supplementation significantly increased the final weight, weight gain and specific growth rate compared with fish fed diets without NE (P<0.05). Interestingly, dietary NE supplementation significantly improved the feed conversion ratio (P<0.05). The supplementation of diets with NE significantly reduced the cholesterol levels (P<0.05). The activities of catalase, superoxide dismutase and glutathione peroxidase significantly decreased as dietary lipid supplement levels increased, but they were significantly improved by dietary NE supplementation (P<0.05). The results also show that the liver malondialdehyde (MDA) content significantly increased as lipid supplement levels increased, while NE supplementation resulted in significantly decreased MDA levels (P<0.05). As dietary lipid supplement levels increased, the crude lipid content of the fillet significantly increased in fish fed a diet supplemented with NE (P<0.05). Moreover, lipase activity was significantly increased when the diet was supplemented with NE (P<0.05). A total of 20 fatty acids were measured in the fillets of fish fed diets supplemented with different lipid levels and NE. Significantly higher levels of polyunsaturated fatty acids, particularly linoleic acid (18:2 n6c), γ-linolenic acid (C18:3 n6) and α-linolenic acid (C18:3 n3), were found in fish fed diets supplemented with NE. In summary, dietary NE supplementation has a positive effect on feed digestion and absorption, resulting in improved fish growth performance, immune response and nutritional value of the muscle due to increased essential fatty acid contents. NE supplementation is particularly effective when it is added to diets with low lipid levels.

Taxonomic, Temporal, and Spatial Variations in Zooplankton Fatty Acid Composition in Puget Sound, WA, USA

Fatty acid (FA) content and composition of zooplankton in Puget Sound, Washington (USA) was studied to investigate the nutritional quality of diverse zooplankton prey for juvenile salmon (Oncorhynchus spp.) in terms of their essential fatty acid (EFA) content. The study focus was on eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and arachidonic acid (ARA) as these are key FA needed to maintain growth and development of juvenile fish. The different zooplankton taxa varied in their FA composition. Much of the variation in FA composition was driven by 18:1ω9 (a biomarker of carnivory), ARA, DHA, and FA characteristic of diatoms, which are linked to zooplankton diet sources. Gammarid and hyperiid amphipods contained the highest amount of EFA, particularly the gammarid amphipod Cyphocaris challengeri, while shrimp and copepods had much lower EFA content. Crab larvae, which are important prey for juvenile salmon in Puget Sound, had intermediate EPA + DHA content and the lowest DHA/EPA ratio, and were rich in diatom biomarkers. Temporal and spatial trends in zooplankton lipids were less apparent than the taxonomic differences, although the EFA content increased from spring to summer in Cancridae zoeae and the amphipod C. challengeri. These results on taxon-specific EFA content provide baseline information on the nutritional quality of zooplankton that can be applied in food web models. Combining zooplankton fatty acid data (quality) with taxon-specific zooplankton biomass data (quantity) enables development of new, sensitive indicators of juvenile fish production to help assess recent declines in salmon production in the Pacific Northwest and predict future adult returns.

Species validation, growth, reproduction and nutritional perspective of Indian pompano, Trachinotus mookalee–A candidate species for diversification in coastal mariculture

The Indian pompano Trachinotus mookalee can be an ideal and alternative marine candidate species for aquaculture with its cultivable traits. The study aimed to understand growth, reproductive and nutritional potentials of the fish in different culture environments and also for species validation to avoid ambiguities in further research. Wild collected broodfishes have confirmed that the species is genetically and morphologically differentiated from its four closely related species of genus Trachinotus. The studied fish growth was significantly (P < 0.05) influenced by culture environments, where the maximum growth was attained in cage (684.9 ± 14.0 g), followed by pond (564 ± 13.6 g), Recirculating Aquaculture System (RAS) (471.5 ± 10 g) and the least in indoor Fibre-reinforced plastic tank (294.65 ± 10.35 g) for 12 months of culture. Growth data indicated presence of high genetic growth potential in the species, which could be improved with suitable intervention in abiotic factors. Studied reproductive sex steroid hormone concentrations varied in the fish and were matched with the different maturity stages confirmed by gonadal development stages. The sexually mature male and female fish found with elevated levels of testosterone (2.34 ± 0.25 ng/ml) and 17-β estradiol (2.56 ± 0.95 ng/ml), respectively, which indicated that sex differences has been persisting in the species and the steroid level could act as marker in assessing the maturity status of male and female fish. The gonadal developmental stages indicated that the fish became functional male and female brooders after 26 and 29 months of culture in sea cages, respectively. Nutritional contents of the fish was significantly (P < 0.05) influenced by the culture environments. Crude protein was maximum in cage reared fish (18.96 ± 0.36%), whereas fish in RAS exhibited high crude fat (6.87 ± 1.05%). Palmitic (C16:0) and glutamic acids were the maximum abundant fatty acid and amino acid, respectively in all the cultured environments. However, the content of essential polyunsaturated fatty acids (DHA and EPA) was significantly high in wild fish. Phosphorus and iron were the maximum abundant major and micro minerals, respectively in all the culture systems. With excellent traits linked with aquaculture, the species could be the most suitable candidate for species diversification in coastal mariculture. The species deserves further studies on the basis of influence of abiotic factors on different traits.

Evaluation of the quality characteristics of protein cubes supplemented with moringa leaf (Moringa oleifera Lam.) and green tea powders

The biological activities of moringa leaf and green tea powders were compared, and the quality characteristics of protein cubes supplemented with these powders (5% and 7.5%, respectively) were evaluated. The green tea powder had higher chlorophyll, essential fatty acid, total polyphenol (TPC), and flavonoid (TFC) contents than the moringa leaf powder. The green tea powder had higher levels of 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity (DPPH RSC), Trolox equivalent antioxidant capacity (TEAC), and ferric reducing antioxidant power (FRAP) than the moringa leaf powder, indicating higher antioxidant activity. The green tea powder inhibited the nitric oxide production to a greater extent than the moringa leaf powder. The protein cubes that were supplemented with either the moringa leaf or green tea powders had smaller volumes and were harder, chewier, and more easily fractured than the control protein cubes. The protein cubes supplemented with green tea powder had higher TPC and TFC, exhibited higher antioxidant activity (p&lt;0.05), and had better sensory properties in terms of aroma, taste, and texture than the protein cubes supplemented with moringa leaf powder; however, the differences were not significant(p&gt;0.05). The results of the present study indicate that green tea is a more suitable health-oriented functional additive than moringa leaf in the development of high-protein and nutrient-rich bakery products with high consumer acceptability as well as antioxidant and anti-inflammatory properties.

Comparative analysis of the microbial community and nutritional quality of sufu.

Sufu is a type of fermented food with abundant nutrients and delicious taste. It is made from the fermentation of tofu by various microorganisms. In this study, three types of sufu were prepared through natural fermentation: (NF), single‐strain fermentation (SF), and mixed‐strain fermentation (MF). Microbial species, amino acids, and fatty acids were identified to investigate dynamic changes in nutritional quality and microbial flora in sufu. The results showed that the number of microbial species in NF sufu was the highest (n = 284), whereas that in SF sufu was the lowest (n = 194). Overall, 153 microbial species were found in all three types of sufu. Relative abundance analysis also revealed that Tetragonococcus, Bacillus, Acinetobacter, and Staphylococcus were the main bacteria in sufu. However, there was a large number of harmful bacteria such as Enterococcaceae in NF sufu. The levels of various nutrients were low in SF sufu, whereas the contents of protein and soy isoflavones were higher in NF and MF sufu. Seventeen kinds of amino acids were detected, comprising seven essential amino acids and ten other amino acids. The contents of essential amino acids and essential fatty acids were higher in MF sufu than the other two types, resulting in its high nutritional value. The sufu produced through the three fermentation methods differed significantly (p < .05) in terms of microbial flora and nutritional quality.

Nutrition status of adults with phenylketonuria treated with pegvaliase

BackgroundPegvaliase is an enzyme substitution therapy that reduces blood phenylalanine (Phe) in adults with phenylketonuria (PKU), and often allows normalization of protein intake (≥0.8 g protein/kg).Here we examine the nutrition status of adults with PKU consuming a normal protein intake without medical food after being treated with pegvaliase for ≥1 year. MethodsA cross-sectional study evaluating nutritional intake (3-day food record and food frequency questionnaire), anthropometrics, laboratory indices of protein, micronutrient, and essential fatty acid (EFA) status, and questionnaires evaluating food neophobia and Epicurean eating pleasure. ResultsParticipants (n = 18, 61% female) started pegvaliase 4.9 ± 2.1 years prior to enrollment and were aged 38.2 ± 8.8 years with a mean BMI of 29.2 ± 4.1 kg/m2. Participants consumed a mean of 73.2 ± 17.6 g protein/d (1.0 ± 0.3 g/kg/d). Eleven participants had low blood Phe (<30 μmol/L) with adequate protein intake and normal indices of protein status. Micronutrient and EFA concentrations were normal except for mildly low vitamin D (<30 ng/mL, n = 12). Intakes of sodium, saturated fat, and added sugars exceeded recommendations for healthy adults, though mean diet quality was comparable to a US adult reference population. Lower food neophobia scores correlated with an increased aesthetic appreciation of food. However, 53% of participants self-reported having moderate (n = 6) to high (n = 3) food neophobia. DiscussionParticipants treated with pegvaliase consumed an unrestricted diet with adequate dietary protein and, overall, had normal protein, micronutrient, and fatty acid status. Despite low blood Phe, protein nutriture was not compromised. While nutritional deficiencies were not identified, diet quality was suboptimal and some participants reported food neophobia. Nutrition education remains an important component of care as patients adapt to a normal diet.

Effect of Exogenously Applied Methyl Jasmonate on Yield and Quality of Salt-Stressed Hydroponically Grown Sea Fennel (Crithmum maritimum L.)

Salt stress is one of the main limiting factors for plant growth and crop yield. Halophytes have been postulated as a new food source since they are able to grow under saline environments and have suitable minerals and bioactive compounds. See fennel Crithmum maritimum L. is a facultative halophyte moderately tolerant to salinity. This study was carried out in order to determine the effect spraying methyl jasmonate (MeJa) on the leaves had on the growth and nutritional quality of NaCl-treated sea fennel plants grown in a hydroponic system. For that, the seedlings were treated with (a) 0.5 mM MeJa, (b) 150 mM NaCl, and (c) 0.5 mM MeJa + 150 mM NaCl. The results showed that NaCl reduced the shoot biomass of baby leaf plants, but the addition of MeJa enabled partial recovery. At the same time, when compared with the plants treated only with NaCl, MeJa favoured the Ca and K uptake and translocation to the leaves of saline-treated plants. However, MeJa did not reduce Na levels. In all treatments, nitrate and nitrite ions were in the range of the acceptable daily intake (ADI) and essential fatty acid content was elevated, although the addition of MeJa to NaCl-treated plants reduced linolenic and linoleic acid contents as compared to the plants treated only with NaCl. Total phenolic compounds were not recovered by MeJa after their decrease by salinity and no differences in antioxidant activity was found between treatments. However, all the plants maintained their antioxidant nutritional properties and increased total flavonoids after MeJa spraying to NaCl-treated plants. These results showed that MeJa spraying alleviated the negative effects of salt stress in C. maritimum grown in floating systems, improving the growth of their edible parts and increasing the total flavonoid and mineral content without affecting the total antioxidant capacity of the plant.

Effects of cod liver oil fortification on the quality aspects of mince sausages from Indian Major Carp ( Labeo rohita )

Fortification of mince with PUFA-containing oil is an innovative area in product development. In this study, functional sausages were developed from Rohu mince with different concentrations of cod liver oil (CLO) and evaluated for their physiochemical and fatty acid compositions. Fortification of mince sausage with 4% CLO showed highest gel strength (323.58 g.cm) to the product. The CLO product had a higher n-3/n-6 ratio (1.79–3.65 versus 1.19 in control). The electrophoretic pattern of proteins showed the polymerization of proteins in the presence of CLO. The scanning electron microscopy revealed fine texture, absence of more voids, and homogenous structure in 4% CLO-fortified sausages. Hardness values are improved by the addition of CLO with a positive whiteness index and showed better sensory scores during the study period. Therefore, fortification of mince sausages with CLO could improve the health benefits by providing the optimal concentration of essential fatty acids along with good consumer acceptance. Practical applications The mince from Indian Major Carp, Rohu, can be successfully utilized for the development of sausages like ready-to-eat products. The fortification of mince sausages with cod liver oil (CLO) would provide an optimum concentration of essential fatty acid to offer multiple health benefits. The present study was able to achieve a ratio (n-6/n-3) of 0.55 to 0.27 in CLO-fortified sausages, which was considered optimal for human health.

Stable isotope and fatty acid analyses reveal significant differences in trophic niches of smooth hammerhead Sphyrna zygaena (Carcharhiniformes) among three nursery areas in northern Humboldt Current System.

Fishery pressure on nursery areas of smooth hammerhead in northern Peruvian coast have become a serious threat to sustainability of this resource. Even though, some management actions focused on conservation of the smooth hammerhead populations were proposed in recent years, their scientific foundations are often limited, and biomass of smooth hammerhead in Peruvian waters continues to decrease. To inform management and conservation, this study aims to evaluate the trophic niche of smooth hammerhead juveniles from three nursery areas in the northern Peruvian coast using stable isotope and fatty acid analyses. First, we compared the environmental characteristics of each nursery area (i.e., sea surface temperature and chlorophyll-a concentration) and concluded that nursery areas differed significantly and consistently in sea surface temperature. Subsequently, we evaluated isotopic composition of carbon and nitrogen and fatty acid profiles of muscle and liver tissues collected from juvenile smooth hammerhead from each nursery area. We found that juvenile smooth hammerhead captured in San José were enriched in heavier 13C and 15N isotopes compared to those captured in Máncora and Salaverry. Furthermore, the broadest isotopic niches were observed in juveniles from Máncora, whereas isotopic niches of juveniles from Salaverry and San José were narrower. This difference is primarily driven by the Humboldt Current System and associated upwelling of cold and nutrient rich water that drives increased primary production in San José and, to a less extent, in Salaverry. Compared to smooth hammerhead juveniles from Máncora, those from San José and Salaverry were characterised by higher essential fatty acid concentrations related to pelagic and migratory prey. We conclude that smooth hammerhead juveniles from three nursery areas in the northern Peruvian coast differ significantly in their trophic niches. Thus, management and conservation efforts should consider each nursery area as a unique juvenile stock associated with a unique ecosystem and recognize the dependence of smooth hammerhead recruitment in San José and Salaverry on the productivity driven by the Humboldt Current System.

Characterizing Sections of Elk Velvet Antler by pH and Mineral, Fatty Acid, and Amino Acid Composition

This study was conducted to determine the composition change in different sections of elk velvet antler at 90 days. The following parameters were analyzed: moisture, crude protein, crude fat, crude ash, crude fiber, pH, minerals (Ca, P, K, Mg, Fe, Mn, Zn, Cu, and Pb), amino acids, and fatty acids. Dry matter, crude fiber, and crude ash contents were higher in the base of the antlers and lower in the tip. In contrast, crude protein and crude fat contents were highest in the tip. Moisture content was high in the upper and medium sections of the antlers, but the difference was not significant. Calcium (Ca), phosphorus (P), and magnesium (Mg) contents were high in the base of the antlers, potassium (K) content was high in the tip of the antlers, and zinc (Zn) content showed no difference between antler sections. Saturated fatty acid content was highest in the base of the antlers, whereas unsaturated fatty acid content was highest in the tip. Among unsaturated fatty acids, monounsaturated fatty acid content was high in the tip of the antler, whereas polyunsaturated fatty acid content was high in the upper section of the antler. Essential amino acid content was highest in the upper section of the antler, whereas non-essential amino acid content was lowest in the base. Crude protein, crude fat, K, and unsaturated fatty acid content tended to be high in the tip of the antler, and essential amino acid and polyunsaturated fatty acid contents tended to be high in the upper section of the antler. The tip of the antler had the highest nutrient content. In order to prevent cardiovascular disease, consumption of a certain amount of polyunsaturated fatty acids and amino acids from the upper section of the antler could be beneficial in terms of pharmacological efficacy.

Variables Selection in the Ultraviolet, Visible and Near Infrared Range for Calibration of a Mixture of Vegetable Oils by Absorbance Spectra

The aim of the work was a multivariate calibration of the concentration of unrefined sunflower oil, considered as adulteration, in a mixture with flaxseed oil. The relevance of the study is due to the need to develop a simple and effective method for detecting the falsification of flaxseed oil which is superior in the content of essential polyunsaturated fatty acids to olive oil. A few works only are devoted to identifying adulteration of flaxseed oil, unlike olive oil.Multivariate calibration carried out using a model based on the principal component analysis, cluster analysis and projection to latent structures of absorbance spectra in UV, visible and near IR ranges. Calibration uses three methods for spectral variables selection: the successive projections algorithm, the method of searching combination moving window, and method for ranking variables by correlation coefficient.The application of the successive projections algorithm, ranking variables by correlation coefficient and searching combination moving window makes it possible to reduce the value of the root mean square error of prediction from 0.63 % for wideband projection to latent structures to 0.46 %, 0.50 %, and 0.03 %, respectively.The developed method of multivariate calibration by projection to latent structures of absorbance spectra in UV, visible and near IR ranges using the spectral variables selection by searching combination moving window is a simple and effective method of detecting adulteration of flaxseed oil.

Quantitative analysis of bio-active phytochemical(s) in selected scented rice varieties (Oryza sativa) reveals its intake towards advantage against metabolic disorders

Based on ancestral knowledge towards health beneficial effect, the current study led to investigate the content of bioactive phytochemical(s) in the scented rice (variety “joha”) grain indigenous to the NER-India and their effects on the enzyme involved in metabolic disorders and cellular glucose uptake efficacy. Results of both α-glucosidase inhibitory activity and the glucose uptake assay-guided fractionation based on column purification followed by spectral data analysis confirmed the presence of two major bioactive compounds as linoleic (ω-6-fatty-acid) and linolenic (ω-3-fatty-acid) acid. Quantitative analysis showed that linolenic acid content in scented rice verities was 2.1±0.3 mg per gram, which is ~1.6 fold higher than its content in non-scented rice variety from the same region. The relative ratio (ω-6/ω-3) of these two essential unsaturated fatty acids content in the scented rice variety seeds was calculated as 23.5±0.6 while, in non-scented rice variety seeds, it was 31.6. Chemical fingerprinting by GCMS and HPLC of methanolic extract of selected rice samples revealed the presence of various fatty-acid along with other volatile and polar compounds. In conclusion the scented rice seeds content a better ratio of two essential unsaturated fatty acid compare to non-scented rice seed, which may be an effective choice as diet towards controlling metabolic disorder.

Comparison of growth performance of live feed microalgae and rotifer (Brachionus sp.) under different feeding medium in outdoor culture condition

Live feed is the basic food source and nutrient security for successful seed production of any commercially important aquaculture species of fishes, mollusks and crustaceans. Both plant and animal originated aquatic microscopic organisms are generally termed as live food. They are the basic food items in early stages (larval stage) of life cycle due to small sizes, easy digestibility and enriched in nutrients. The Nannochloropsis sp.; Nannochlorum sp. and Tetraselmis sp. are rich with relatively high content of essential fatty acids in comparison to other marine algae. Likelihood, the rotifer Brachionus sp. is ideal feed item for brackishwater finfish and mud crab larvae rearing due to its special features like rapid reproduction, slow movement, suitable size and easy digestion by the newly hatched larvae. The present study is the report on comparison of growth performance of live feed (microalgae and rotifer) in outdoor culture condition. The study was conducted at the hatchery complex of Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna. In outdoor culture condition the growth pattern obtained for three microalgae were Tetraselmis sp.&gt; Nannochlorum sp.&gt; Nannochloropsis sp. Highest average growth 6.87×106±1.97×106, 6.91×106±1.69×106 and 6.23×106±1.53×106 was observed for Nannochloropsis sp., Nannochlorum sp. and Tetraselmis sp. respectively in trial 3. Average growth of rotifer (Brachionus sp.) found highest 189±18.10 ind./ml at combined media of yeast and microalgae and lowest growth 119.67±17.60 ind./ml noticed with baker's yeast media. Nannochloropsis sp. contains more energy among all microalgae but Tetraselmis sp. carry highest level of protein. The highest level of protein 56.3±0.18% found in rotifer enriched with microalgae+fish oil and lowest protein content noticed with microalgae enrichment media. So, research finding suggests that, treatment T3 would be the best recommendations for rotifer culture.

Oil Recovery from Jalapeño Pepper By-Products and Analysis of the Industrial Scalding Process on Its Nutraceutical Potential

Vegetable by-products represent a valuable source of compounds with biological activity that could be used in food and cosmetics industries. The objective of this work is the valorization through the lipophilic nutraceuticals characterization and in vitro antioxidant activity of Jalapeno pepper industrial by-product oil, and the remainder flour from oil extraction. Jalapeno pepper by-products and whole fruit from two regions: Chihuahua and Sinaloa States from Mexico were obtained from a local industry. From the whole fruit it was prepared a simulated raw by-product, in order to use it to evaluate the effect of industrial scalding process on the by-product. The Jalapeno pepper by-product contain around 22% of oil with important lipophilic nutraceuticals, the oil has a greenness color, and remains attractive concentrations of essential fatty acids, more than 70% of linoleic acid; β-carotene up to 1.85 mg/100 g of oil; α-tocopherol (46.12 mg/100 g of oil) up to four times more than red pepper seeds (C. annuum). Moreover, two and a half and five times more capsaicin and dihydrocapsaicin, respectively, were detected in the oil fraction, than the reported before in no polar extract from whole fruit of C. frutescens. The scalding process reduces the β-carotene, capsaicinoids, and total phenolic content and antioxidant activity of the oil fraction, but despite of this, Jalapeno pepper by-product oil and remainder flour contain bioactive compounds at similar concentrations to previously reports in other peppers. The Jalapeno pepper by-products derived by the food industry have the potential to be used for the recovery of an oil with nutraceutical potential.

Biological Activity of Sacha Inchi (Plukenetia volubilis Linneo) and Potential Uses in Human Health: A Review.

SUMMARYSacha inchi (Plukenetia volubilis Linneo) is an ancestral plant originating in the Amazon jungle that has been adopted as a food source due to its high nutritional value, which has gradually been recognized to have potential benefits for human health. Diverse prospective studies have evaluated the effect of consuming components from the plant, derivatives from its seeds, leaves and shell on preventing the risk of cardiovascular disease, chronic inflammatory disease, dermatitis and controlling tumor proliferation, especially given its recognized high content of essential fatty acids, phenolic compounds and vitamin E, showing antioxidant, hypolipidemic, immunomodulation and emollient activity, as well as the capacity to remove heavy metals from aqueous solutions. This review offers a complete description of the existing information on the use and biological activity of P. volubilis L., based on its essential lipid components and evidenced on its use in the field of human health, in prevention, therapeutic and nutritional contexts, along with industrial uses, making it a promising bioresource.

ШИКІ ЫСТАЛҒАН ҚҰС ЕТІНІҢ ТАҒАМДЫҚ ЖƏНЕ БИОЛОГИЯЛЫҚ ҚҰНДЫЛЫҒЫН БАҒАЛАУ

In solving the problem of providing the population with high-grade food, an important role belongs to the domestic poultry processing industry as the most effectively developing in the world. At the same time, the market of delicatessen products from poultry meat is not large and it needs to expand the range. Of particular interest is the development of raw smoked products using starter cultures. A comparative commodity science assessment of the biological usefulness of raw smoked whole- muscle poultry meat product is given. As a result of the commodity quality assessment, it was proved that the types of raw smoked products from poultry meat offered to consumers have the best organoleptic properties and nutritional value. In the future, when calculating the biological value of raw smoked products, data from the amino acid score of experimental and control samples were used.Тhe biological value of meat products primarily depends on the quantitative content of essential amino acids and polyunsaturated fatty acids, the degree of availability of nutrients to digestive enzymes and the level of assimilation in the body. In connection with the above, on certain types of experimental meat products, a series of experiments were consistently presented, the results of which are presented in this article. Key words: poultry meat, starter cultures, amino acid score, raw smoked products.

STUDY OF NUTRITIONAL CONTENTS OF SEA URCHIN GONAD FROM DRINI BEACH, GUNUNG KIDUL, YOGYAKARTA

ABSTRACTThis paper aims to determine the types and contents of protein, fatty acids, amino acids and protein in the gonads of Arbacia lixula, Colobocentrotus atratus, Heterocentrotus trigonarius and Echinotrix diadema from the waters of Drini, Gunung Kidul, Yogyakarta. Kjedal method was used to analyze the protein content, while GC and HPLC methods were employed to analyze amino acids in this study. The results showed that the protein contents of each sample, consequently from highest to lowest, were Heterocentrotus trigonarius (13.3 %), Echinotrix diadema (10.86), Colobocentrotus atratus (10.41) and Arbacia lixula (9.90%). Amino acids analysis from all identified both saturated fatty acids, consisting of lauric acid, myristic acid, palmitic acid, stearic acid, arachidonic acid, lignoceric acid, as well as non-saturated fatty acids, consisting of palmitoleic acid, oleic acid, linoleic acid, erucic acid, EPA, and DHA. The highest contents of non-saturated fatty acids were identified in Colobocentrotus atratus (434.14 mg/100g) and the lowest content in Arbacia lixula (197.71 mg/100g). The highest percentage of essential fatty acids was found in Heterocentrotus trigonarius (0.29%), whereas the lowest was found in Echinotrix diadema (0.19 %). It is concluded that the gonad of Heterocentrotus trigonarius showed the highest protein and essential fatty acids contents. This study also found that Colobocentrotus atratus sea urchin gonads possess the highest content of non-saturated fatty acids (434.14 mg/100g). Keywords: amino acid, fatty acid, protein, sea urchin gonad.

Taxonomic Shift Over a Phosphorus Gradient Affects the Stoichiometry and Fatty Acid Composition of Stream Periphyton.

Phosphorus enrichment of stream ecosystems generally increases primary production in the benthos, but the consequences of eutrophication for the nutritional quality of periphyton for grazers are less clear. On short timescales, high phosphorus inputs may lead to reduced C:P ratios and high essential fatty acid contents of periphyton, which are both considered important determinants of food quality for grazers. However, nutrient enrichment may alter the taxonomic composition of periphyton and favor the growth of less palatable algal taxa. In this study, periphyton was grown under a gradient of dissolved phosphorus availability from 5 to 100µg P·L-1 , to investigate eutrophication effects on periphyton taxonomy, C:N:P stoichiometry, and fatty acid composition. After 1month, periphyton grown under oligotrophic conditions was mainly composed of diatoms (~86%). With increasing phosphorus availability, diatoms were gradually outcompeted by chlorophytes and cyanobacteria, which were the predominant taxon under eutrophic conditions. Unexpectedly, periphyton C:P ratios increased with greater phosphorus supply, from ~280 under oligotrophic conditions up to ~790 at 100µg·L-1 , reflecting a tendency of chlorophytes and cyanobacteria to produce more biomass per unit of assimilated phosphorus compared to diatoms. Periphyton content of essential polyunsaturated fatty acids relative to biomass followed a unimodal relationship with phosphorus availability and peaked at intermediate phosphorus levels, likely as a result of both taxonomic and nutrient effects. Our results demonstrate that phosphorus-driven eutrophication of freshwater ecosystems may worsen periphyton nutritional quality due to taxonomic sorting, which may further lead to lower growth and reproduction of herbivores.

Impact of environmental conditions on phospholipid fatty acid composition: implications from two contrasting estuaries

Phospholipid (PL) composition has a tremendous influence on the cell integrity and physiological competency. At the same time, plankton PL make important metabolic fuels for higher trophic levels. The goal of this study was to identify environmental control on PL production and their molecular identity of the suspended particles in two different estuaries. We conducted research in subtropical, eutrophic Wenchang River Estuary in China and temperate pristine, mesotrophic Krka River Estuary in Croatia. In agreement with the more abundant phytoplankton, PL concentrations were much higher in the Wenchang River Estuary (30.3–178.2 μg L−1) than in the Krka River Estuary (8.4–18.8 μg L−1). Given that six PL classes investigated (phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidic acid (PA), phosphatidylinositol (PI), and phosphatidylserine (PS)) have different roles in the cell, we expected their different fatty acid composition in different environments. We found small differences in the fatty acid composition of PC, PG, and PI between two estuaries. These results suggest that the essential fatty acid compositions of these PL in estuarine plankton are relatively constant in order to preserve membrane functions and/or cell processes in which they are involved regardless of environmental conditions. In contrast, PE, PA and PS fatty acid composition substantially differed between two estuaries as well as throughout the salinity gradient in each estuary. This suggests the adaptability of plankton to remodel these PL depending on the environmental conditions and the plankton community structure. Good environmental conditions (favorable N/P ratio, temperature) are important for increased PL content (% in POC and total lipids) in estuarine plankton and increased essential polyunsaturated fatty acid content in PL, which is beneficial to higher trophic levels.