Essential Fatty Acid Content Research Articles

Comparison of volatile compounds and fatty acids of jujubes (Ziziphus jujuba mill.) before and after blackening process

ABSTRACT Blackened jujube, as an emerging fruit processed product, was obtained from red jujube by a blackening processing of Maillard reaction with high humidity and temperature. This study was to compare the volatile compounds (VOCs) and fatty acids (FAs) between red jujubes (RJs) and blackened jujubes (BJs) of six cultivars (JZ, MZ, JSXZ, HZ, YHDZ and TZ) using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) and GC-MS respectively. A total of 56 VOCs were detected in these six cultivars (RJs and BJs), and their types and contents were analyzed. A total of 22 FAs, including 12 saturated fatty acids (SFAs) and 10 unsaturated fatty acids (USFAs), was determined in the RJs and BJs, The content of essential FAs in BJs was higher. B-JZ had the greatest increase in linoleic and ɑ-linolenic from 5.3 mg/100 g to 10.0 mg/100 g and from 3.4 mg/100 g to 5.1 mg/100 g, respectively. It indicated that JZ is better for improving flavor and the composition of FAs by blackening process than other five cultivars. In addition, FAs and VOCs were found to have a linear correlation with color. The types of VOCs and FAs between red and blackened jujubes were also discriminated using principal component analysis (PCA).

The Nutritional Properties, Chemical Compositions, and Functional Characteristics of the Aerial Parts of Adonis coerulea.

The nutrition and active compounds from plants are very important to regulate the immunity of the body by improving the oxidant and inflammatory response. In this article, we aimed to investigate the nutritional profile and the phytochemical compositions of Adonis coerulea; the functional characteristics and its possible mechanism were studied. Results showed that the aerial parts of Adonis coerulea (ACAP) contained the abundant of proteins (16.15%) and the minerals (31.02.09 mg/100 g dried ACAP); promisingly, the content of essential amino acids (8.25%) and fatty acids (13,220.45 mg/100 g) also were obtained to regulate the immunity and prevent some chronic diseases. The methanol extract of ACAP played the anti-inflammatory activity via peroxisome proliferators-activated receptor (PPAR)-γ-mediated nuclear factor kappa B (NF-κB) pathway. Among the 18 identified compounds, linolenic acid from fatty acids and licochalcone A were active compounds by inhibiting nitric oxide (NO) production of RAW264.7 cells induced by lipopolysaccharide (LPS). The alleviation of inflammatory response results in the decrease of oxidative stress; ACAP showed the antioxidant activity by attenuating antioxidant enzymes, improving mitochondrial membrane potential and reactive oxygen species. These results highlight the potential of A. coerulea as a source of active ingredients in pharmaceutical industries.

65 Evaluation of Essential Fatty Acids in Lactating Sow Diets on Sow Reproductive Performance, Colostrum and Milk Composition, and Pre-Weaning Litter Growth and Survivability

Abstract Mixed parity sows (n = 3,451; PIC, Hendersonville, TN) and their litters were used to evaluate the effects of essential fatty acid (EFA) intake on sow reproductive performance, piglet growth and survivability, and colostrum and milk composition. At approximately d 112 of gestation, sows were randomly assigned to 1 of 4 corn-soybean meal-wheat-based lactation diets with 0.5 (Control) or 3% choice white grease (CWG), 3% soybean oil (SO), or a combination of 3% soybean oil and 2% choice white grease (Combination). Thus, sows were provided diets with low EFA [linoleic acid (LA) and α-linolenic acid (ALA)] in diets with CWG or high EFA in diets with SO. Lactation ADFI increased (P < 0.05) for sows fed the Combination and CWG diets compared with sows fed the Control or SO diet. Daily LA and ALA intake of sows fed the Combination and SO diets were greater (P < 0.05) than sows fed 0.5 or 3% CWG. Overall, sows consuming high EFA from the Combination or SO diets produced litters with heavier (P < 0.05) piglet weaning weights and greater (P < 0.05) litter ADG when compared with litters from sows fed diets with low EFA provided through CWG. There was no effect of sow EFA intake on piglet survivability (P > 0.10). Lactation diet EFA concentration did not influence colostrum or milk dry matter, crude protein, or crude fat content (P>0.10). However, LA and ALA content in colostrum and milk at weaning increased (P < 0.05) in response to elevated dietary EFA from SO. There was no evidence for differences (P > 0.10) in subsequent sow reproductive or litter performance due to previous lactation EFA intake. In conclusion, increased LA and ALA intake provided by SO during lactation increased overall litter growth and pig weaning weights but did not affect piglet survivability or subsequent performance.

Biochemical composition and quality of wild-caught adult mitten crabs from three river basins

The development and utilization of new germplasm resource is an important basis for promoting mitten crab industrial development. A total of 90 wild-caught mitten crabs were collected from Suifenhe (SFH), Liaohe (LH) and Nanliujiang (NLJ) river basins. This study investigated the edible yield, color, proximate composition, fatty acid, and free amino acid composition of wild-caught mitten crab edible tissues from three river basins. Results illustrated that SFH crab had significantly higher meat yield (MY) compared with LH and NLJ crabs (P < 0.05). Higher proportion of fatty acids and free amino acids were also observed in SFH crab, e.g. 22:6n3 (DHA), 20:5n3 (EPA), n-3 unsaturated fatty acid (PUFA), n-3/n-6 PUFA, ∑DHA+EPA, and total free amino acids (∑TFAA) in gonad. LH crab had higher concentration of total essential fatty acids (∑EFA), FAAs and h/H in hepatopancreas, while NLJ crab accumulated high content carotenoids in the carapace and hepatopancreas, AI and TI in edible tissues. In summary, significant differences of biochemical composition and quality were found among these three wild-caught mitten crabs, which provides basic materials for the development and utilization of mitten crabs.

Content of industrially produced trans fatty acids in breast milk: An observational study.

Breast milk may contain industrially produced trans fatty acids (TFAs), which can affect the content of essential fatty acids (EFAs). This could have significant implications for the child's development. The fatty acids present in breast milk can be modified by adjusting the mother's diet. The objective of this study was to determine the content of industrially produced TFAs present in colostrum, transitional milk, and mature milk produced by mothers between 18 and 45 years of age in the state of Querétaro, Mexico, based on a longitudinal observational study. The TFA content in the breast milk of 33 lactating women was analyzed using gas chromatography. The mothers’ consumption of TFAs was also estimated by analyzing a log prepared through 24‐hr dietary recall (24HR) obtained in each period. The TFA content in the mothers’ diet was similar across the colostrum, transitional milk, and mature milk phases: 1.64 ± 1.25 g, 1.39 ± 1.01, and 1.66 ± 1.13 g, respectively. The total TFA content was 1.529% ± 1.648% for colostrum; 0.748% ± 1.033% for transitional milk and 0.945% ± 1.368% for mature milk. Elaidic acid was the TFA in the highest concentration in all three types of milk. No correlation was found between the content of industrially produced TFAs in breast milk and the anthropometric measurements of the mother or between the estimated consumption of TFAs and the content of TFAs in breast milk. Elaidic acid and total content of TFAs were negatively correlated (p < .05) with the content of docosahexaenoic acid (DHA) (0.394 ± 0.247) (R = −0.382) in colostrum. The concentration of TFAs was found to correlate with the composition of EFAs in milk.

Evaluation of soybean stinkbug (Riptortus pedestris) powder, as a food ingredient and its nutritional composition

Riptortus pedestris is a potential edible insect, and its powder can be considered as an ideal nutrient source. This study sought to determine the nutritive quality of the powder from Riptortus pedestris. The protein and crude fat content of dried R. pedestris powder were 77.61 g 100 g&lt;sup&gt;–1&lt;/sup&gt; and 18.01 g 100 g&lt;sup&gt;–1&lt;/sup&gt;, respectively. It contained 18 amino acids and 6 fatty acids comparable to eggs and soybean. The total essential amino acid contents could meet the requirement of preschool children and adults. The unsaturated fatty acid content in R. pedestris powder was comparatively more than saturated fatty acid content. Essential unsaturated fatty acids content, especially linoleic acid, was quite high than egg or some other edible stinkbugs. Additionally, R. pedestris powder was rich in various mineral substances, and the macro-element and micro-element contents were more balanced than other edible stinkbugs. These results suggest that R. pedestris powder could be a highly nutritious, alternative novel nutrition source for humans.

Simultaneous Quantification of Organic Acids in Tamarillo (Solanum betaceum) and Untargeted Chemotyping Using Methyl Chloroformate Derivatisation and GC-MS.

Sixteen organic acids were quantified in peel and pulp of Amber, Laird’s Large and Mulligan cultivars of tamarillo using GC-MS. Fourteen of these compounds had not previously been quantified in tamarillo. An untargeted metabolomics approach was used in parallel to identify and quantify 64 more metabolites relative to the internal standard, indicating abundances of glutamic acid, pro-line, aspartic acid and γ-aminobutyric acid as well as lower concentrations of several other essential fatty acids and amino acids. The main findings were that total organic acid concentration was significantly higher (p < 0.05) in pulp than in peel, with the highest concentration seen in Mulligan pulp (219.7 mg/g DW). Remarkably, after citric acid, the potent bactericide itaconic acid was the second most abundant organic acid. At least 95% of organic acids in tamarillo were one of these two acids, as well as cis-aconitic, malic and 4-toluic acids. Differences between cultivar chemotypes were as substantial as differences between tissues. These results suggest that the bitter flavour of the peel does not result from organic acids. The combination of targeted and untargeted metabolomics techniques for simultaneous qualitative and quantitative investigation of nutrients and flavours is efficient and informative.

Essential fatty acids composition and oxidative stability of frozen minced carp meat

ABSTRACT This study aimed to introduce minced common carp (Cyprinus caprio L.) meat as a nutritional valuable by-product from carp filleting. This research was focused on fatty acid composition and considered its sensitivity to oxidation during frozen storage (−20°C). Additionally, copper chloride was used to magnify possible oxidation reactions. A better understanding of minced carp meat quality and deterioration during frozen storage could help to promote the usage of this underestimated by-product. The utilization of this by-product for human consumption could be a useful way to increase the carp aquaculture economy and sustainability. The fatty acid profile showed a favorable content of essential fatty acids, as well as a beneficial ratio of ω-3/ω-6 polyunsaturated fatty acids. Furthermore, during 4 months of frozen storage (−20°C), no significant changes were detected in fatty acid composition. Negligible changes were observed in the oxidation of lipids and proteins. The nutritional value and storage stability of minced carp meat was shown.

Changes in the fatty acid composition of rapeseed and camelina oil cakes in the extrusion process and their biological effectiveness evaluation

The problems of extrusion process influence on the fatty acid composition of products obtained from the cake of cruciferous oilseeds (rapeseed, camelina) were considered in the article. Cakes of these crops have a wide range of uses for fodder purposes, which indicates their high biological value. The aim of this study was to evaluate the biological effectiveness of rape and camelina cakes before and after the extrusion process accorfding to the calculation of the rationality coefficient of the fatty acid composition. An increase in the content of saturated fatty acids and a decrease in unsaturated fatty acids were revealed during studies after extrusion processing. A decrease in the amount of linolenic acid was also noted in the work. Its amount decreased by 2.24% in rapeseed cake and by 2.63% in camelina cake after extrusion. It was determined that the coefficient of unsaturation of oils in rapeseed and camelina cakes decreases after extrusion by 4 and 3% for rapeseed and camelina cakes, respectively. The calculation of the rationality coefficients of the fatty acid composition showed an increase in the biological efficiency of oils in rapeseed and camelina cake after extrusion. The balance of the fatty acid composition increased after extrusion by 0.07 fractions of a unit in rapeseed cake and by 0.04 fractions of a unit in camelina. The use of the extrusion method in the processing of cruciferous oilseeds makes it possible to expand the range of raw materials for the feed industry, obtain feed that is safe for feeding animals and poultry, does not reduce its quality during storage and increases the energy value of the diet, and provides opportunities for the development of food products with increased content of essential fatty acids as well.

Prymenko V., Sefikhanova K. DEVELOPMENT OF THE CHEESE PRODUCT COMPONENT COMPOSITION WITH VEGETABLE FILLER ENRICHED WITH SELENIUM

The most complete provision of the human body with physiologically useful nutrients in the required quantities can be achieved due to the diversity of the chemical composition of the group of functional products, which determines the actualization of its creation. Approaches, based on experimental-statistical and linear programming methods, are mainly used to design recipes for multicomponent food systems. One of the most common programs for calculating recipes is MS Excel. The aim of the study is to design with the help of MS Excel software a complex multi-component cheese product with vegetable filling, enriched with selenium, which will increase the content of essential fatty acids to a level comparable to human daily needs, reduce cholesterol to WHO recommended consumption, increase in carotenoids, vitamin C, dietary fiber and selenium. Results. A recipe for a multicomponent cheese product enriched with selenium has been developed. An optimized complex cheese product with a vegetable filler based on a multicomponent cheese product enriched with selenium has been designed. The article presents the method of designing new functional food products, the chemical composition of developments, determines the nutritional and energy values of cheese product enriched with selenium, and optimized cheese product with vegetable filler based on it. It was found that the consumption of 100 g of cheese product with vegetable filler is able to meet the daily need for nutrients by 13.4 %, while the multi-component cheese product – by 9.6 %. Conclusions. Development of food products of optimal composition by mathematical modeling allows to reduce financial and time costs for food development, respond to changes in human needs in a man-made society and significantly expand the range of functional, dietary products, aimed at feeding certain groups. Expanding the capabilities of optimization software allows reaching a qualitatively new level in the development of new types of food products with a given chemical composition, consumer and technological characteristics. Thus, the implementation of this task allowed to increase the share of vegetable fat as a source of polyunsaturated fatty acids in the cheese product, antioxidants, some vitamins and cheaper raw materials, by adding selenium-protein dietary supplement and pumpkin puree.

Effect of Spraying with GA&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; on the Quality and Oil Content of Syrian Okra Seeds &amp;lt;i&amp;gt;Abelmoschus esculentus&amp;lt;/i&amp;gt; (L.)

This study aimed to estimate the effect of spraying of okra plants, Abelmoschus esculentus (L.) with GA3 on the quality and oil content of the seeds. For this, the local okra variety was selected and used, which was growing widespread in the coastal region of Syria. This study was carried out in Al-Haffa area, Lattakia governorate, Syria (with 105 MSL elevation) for two seasons 2019-2020. The results showed that the effect of spraying with GA3 was more evident in the quality of the seeds as well as oil content at 75 ppm concentration with 68.7 g weight of 1000 seeds and 5.31% seed oil percentage. This study also showed that oil of local okra variety seed was rich in the essential fatty acids namely, linoleic, palmitic, oleic, stearic and linolenic, treatment with 75 ppm concentration of GA3 was more effective in the seed content of essential fatty acids with 42.30%, 32.55%, 9.20%, 6.73% and 5.72%, respectively. Finally, our results were indicated that treatment of okra plants at 75 ppm of GA3 enhanced the quality and oil content of seeds compared to the other concentrations.

Identification of zooplankton carbon sources using fatty acid biomarkers in lakes and reservoirs at different trophic states in Nanjing and surrounding areas

脂肪酸在生物的摄食活动中相对稳定，可用于辨别消费者的食物来源.不同营养水平的湖泊与水库中浮游动物的碳源组成有差异，因而可能具有不同的脂肪酸组成特征.本研究对南京市及其周边21个湖库的颗粒有机物（POM）、浮游动物的脂肪酸组成进行了对比分析.结果表明，南京市及其周边湖库21个湖库由中营养、轻富营养、中富营养、重富营养共4个营养水平组成.中营养湖库总氮（TN）、总磷（TP）、叶绿素a（Chl.a）、浮游植物生物量均低于富营养湖库，透明度（SD）则相反.中营养湖库中POM脂肪酸的必需脂肪酸（EFA）平均浓度低于富营养湖库；而细菌特征脂肪酸∑C15+∑C17含量高于富营养湖库.随着湖库富营养化，POM中多不饱和脂肪酸（PUFA）浓度逐渐增大，单不饱和脂肪酸（MUFA）浓度则逐渐减少.中营养湖库中浮游动物PUFA浓度普遍低于富营养湖库，∑C15+∑C17含量高于富营养湖库；且浮游动物体内主要来源于藻类的必需脂肪酸EFA/C16：0比值与细菌源脂肪酸（∑C15+∑C17）/C16：0比值均大于其他湖库，表明中营养湖库中浮游动物摄食了较多细菌.随着湖库营养水平升高，浮游动物饱和脂肪酸（SFA）逐渐增多，MUFA浓度逐渐减少，EFA浓度增多，表明浮游动物主要摄食藻类，而外源碳对浮游动物的碳源贡献较小.冗余分析（RDA）结果显示，EFA/C16：0、（∑C15+∑C17）/C16：0与透明度呈正相关，与Chl.a、总磷、磷酸盐呈高度负相关，表明透明度较高、营养水平相对较低的湖库中，浮游动物可能利用较多的藻类与细菌碳；此外，陆源碳转化指标∑C24/C16：0与TN、硝态氮呈正相关性，表明随着营养水平升高，陆源碳对浮游动物碳源贡献呈增加趋势.总体而言，浮游动物更倾向于利用高质量的内源碳，但在内源碳不足以供给水生生物生长需求的中营养水体中，外源碳对生态系统的物质循环和能量流动会产生重要作用.;Fatty acids are relatively stable in the feeding activities of organisms and can be used to identify consumers' food sources. The carbon source composition of zooplankton in lakes and reservoirs with different nutrient levels is different, so they may have different fatty acid composition characteristics. This study compared the fatty acid composition of POM (particulate organic matter) and zooplankton in 21 lakes and reservoirs in Nanjing and its surrounding areas. The results show that the 21 lakes and reservoirs are composed of four nutritional levels:medium nutrition, light eutrophication, medium eutrophication, and heavy eutrophication. The total nitrogen (TN), total phosphorus (TP), chlorophyll-a (Chl.a), and phytoplankton biomass of mesotrophic lakes and reservoirs are lower than those of eutrophic lakes and reservoirs, while the transparency (SD) is the opposite. The average concentration of essential fatty acids (EFA) of POM fatty acids in mesotrophic lakes was lower than that of eutrophic lakes, while the content of bacterial characteristic fatty acids ∑C15+∑C17 was higher than that of eutrophic lakes. With nutrient increase, the concentration of polyunsaturated fatty acids (PUFA) in POM gradually increases, while the concentration of monounsaturated fatty acids (MUFA) gradually decreases. The PUFA of zooplankton in mesotrophic lakes and reservoirs is generally lower than that of eutrophic lakes and reservoirs, and the content of ∑C15+∑C17 is higher than that of eutrophic lakes and reservoirs; and the ratio of essential fatty acids EFA/C16:0, which are mainly derived from algae, and bacterial fatty acids in zooplankton (The ratio of ∑C15+∑C17)/C16:0 is larger than that of other lakes and reservoirs, indicating that zooplankton in the middle-nutrition lakes and reservoirs ingested more bacteria. As nutrient increases, zooplankton saturated fatty acids (SFA) gradually increase, MUFA gradually decreases, and EFA content increases, indicating that zooplankton mainly feeds on algae, while exogenous carbon contributes little to the carbon source of zooplankton. Redundancy analysis (RDA) show that EFA/C16:0, (∑C15+∑C17)/C16:0 are positively correlated with SD, and highly negatively correlated with Chl.a, TP, and phosphate, suggesting that zooplankton consumed more algal and bacterial carbon; and ∑C24/C16:0 is positively correlated with TN, nitrate nitrogen, indicating that zooplankton utilized more terrestrial carbon. In general, zooplankton is more inclined to use high-quality endogenous carbon, but in mesotrophic water bodies where endogenous carbon is not sufficient to supply the growing needs of aquatic organisms. Exogenous carbon plays an important role in the material circulation and energy flow of the ecosystem.

Evaluation of the composition of essential fatty acids in the tissues of rainbow trout (Oncorhynchus mykiss) grown in closed water supply units

Recently, the task of artificial cultivation of especially valuable fish species in aquaculture has become more and more urgent. The value of salmon fish, and in particular rainbow trout Oncorhynchus mykiss (Walbaum, 1792) available for breeding, is largely determined by the high content of biologically active essential and polyunsaturated fatty acids of the group ω-6 and ω-3, necessary for vital activity and not synthesized by the human body. Since the natural stocks of many popular fatty fish have decreased, farmed fish can help meet consumer demand for this product. Based on this, the assessment of the content of the main ω-6 and ω-3 fatty acids in the fat of rainbow trout grown in closed-loop water supply installations (USV) was made. The fat was extracted from the fish by thermal method with further distillation by supercritical fluid extraction on the SFT-150 unit. The ratio of fatty acids in the fish oil sample was determined using the Chromatek-Kristall-5000 M hardware and software complex. The results obtained were compared with the standard for salmon fish oil “Aquaculture products” and “Wild fish”. It is established that trout cultivated in the UZV in the Penza region, in terms of the content of the main essential fatty acids, is not inferior to the quality indicators of the standard in terms of the requirements for salmon fish of “Aquaculture products”. In fish, all essential fatty acids are present in sufficient quantities, the ratio of ω-6 to ω-3 is 2.6:1, which indicates a high nutritional value of the product and allows it to be used in human food to provide the body with essential fatty acids, as well as a raw material for obtaining therapeutic and preventive products.

Investigation of Deep-Sea Ecosystems Using Marker Fatty Acids: Sources of Essential Polyunsaturated Fatty Acids in Abyssal Megafauna.

Abyssal seafloor ecosystems cover more than 50% of the Earth’s surface. Being formed by mainly heterotrophic organisms, they depend on the flux of particulate organic matter (POM) photosynthetically produced in the surface layer of the ocean. As dead phytoplankton sinks from the euphotic to the abyssal zone, the trophic value of POM and the concentration of essential polyunsaturated fatty acids (PUFA) decrease. This results in pronounced food periodicity and limitations for bottom dwellers. Deep-sea invertebrate seston eaters and surface deposit feeders consume the sinking POM. Other invertebrates utilize different food items that have undergone a trophic upgrade, with PUFA synthesized from saturated and monounsaturated FA. Foraminifera and nematodes can synthesize arachidonic acid (AA), eicosapentaenoic acid (EPA), while some barophylic bacteria produce EPA and/or docosahexaenoic acid. FA analysis of deep-sea invertebrates has shown high levels of PUFA including, in particular, arachidonic acid, bacterial FA, and a vast number of new and uncommon fatty acids such as 21:4(n-7), 22:4(n-8), 23:4(n-9), and 22:5(n-5) characteristic of foraminifera. We suppose that bacteria growing on detritus having a low trophic value provide the first trophic upgrading of organic matter for foraminifera and nematodes. In turn, these metazoans perform the second-stage upgrading for megafauna invertebrates. Deep-sea megafauna, including major members of Echinodermata, Mollusca, and Polychaeta display FA markers characteristic of bacteria, foraminifera, and nematodes and reveal new markers in the food chain.

Valorization of the Olive Oil Production Residue: Healthy Ingredient for Developing High Value-Added Spread

This study focused on the development of a new olive-based product enriched with proteins, antioxidant compounds and functional properties, aiming at valorization of the olive residue from the typical olive oil extraction process. Olive oil was extracted using a patented cold pressing machine, resulting in a valuable source of bioactive compounds in the form of olive residue (pulp ~70%, pit- and skin-free). Conventional (solid: water ratio 1:4, 25 °C, 2 h) and HP-assisted (200 MPa, solid:water ratio 1/4, 10 min) de-bittering processes were carried out in order to remove oleuropein content (up to 50%) from olive residue, thus decreasing its bitter taste. The olive spread was prepared with olive residue (50%) and honey (25%), enriched with proteins (24.5%) from nuts. Quality parameters, nutritional attributes, microbiological analysis, phenolic compounds and oxidative stability as well as sensory evaluation of the olive spread were conducted during storage. Its shelf-life was estimated (prediction through accelerated tests) as 9.5 months when stored at 4 °C. The organoleptic evaluation showed that the proposed olive spread had a texture similar to peanut butter and could potentially be consumed as a health-promoting spread due to its high content of proteins (~10 g/100 g), crude fibers (&gt;6 g/100 g) and essential fatty acids.

Crowding stress-related protein markers: New candidates for assessing welfare of largemouth bass reared in an in-pond raceway system

Compared to traditional pond culture, in-pond raceway system (IPRS) is an advanced model, which can reduce waste of water resources and have the characteristics of high efficiency, high yield and environmental protection. However, how to determine the appropriate stocking density has not been studied using protein markers, especially from a welfare perspective. Therefore, this study mainly evaluated the effect of stocking density on the growth, meat quality and liver proteome of largemouth bass (Micropterus salmoides). Largemouth bass (average weight, 14.29 ± 0.26 g) were randomly distributed into three in-pond raceways at three stocking densities: low stocking-density (LSD, 7000 individual/raceway), medium stocking-density (MSD, 20,000 individual/raceway) and high stocking-density (HSD, 30,000 individual/raceway). The growth performance, muscle quality and liver protein expression were analyzed after a 58-day feeding trial. The results showed that the LSD group obtained significantly higher specific growth rate compared to the HSD group. As the density increased, the survival rate of fish decreased from 92.56% to 86.33%, and the feed conversion ratio increased from 1.21 to 1.45. Different stocking densities can significantly affect the contents of essential fatty acids in muscle, but not have any obvious changes on the amino acid pattern in muscle. A total of 109, 35 and 209 differentially regulated liver proteins were detected in LSD vs. MSD, MSD vs. HSD, and HSD vs. LSD groups, respectively. Among them, Hsp70, Grp78, Erp57, Gfpt1, Pyg and Acc1 are involved in stress, glucose metabolism or lipid metabolism, which were confirmed by western blotting. Thus, these proteins could be selected as biomarkers to determine stocking density. The findings will benefit IPRS to balance the yield and welfare of fish according to the market and law changes, beyond profit-seeking in the future.

Lake browning impacts community structure and essential fatty acid content of littoral invertebrates in boreal lakes

Many lakes in the northern hemisphere are browning due to increasing concentrations of terrestrial dissolved organic carbon (DOC). The consequences of lake browning to littoral invertebrates, however, are not fully understood. We analyzed community structure and fatty acid (FA) profiles of littoral invertebrates in humic (DOC-rich) and clear-water lakes in Eastern Finland. We found higher abundance of chironomids (Diptera: Chironomidae) in humic compared to clear-water lakes, whereas stoneflies (Plecoptera) and mayflies (Ephemeroptera: Baetidae) were more abundant in clear-water lakes. Taxon explained 65% of the differences in the FA composition of littoral invertebrates. However, the proportion and content of polyunsaturated FAs of several taxa were significantly higher in clear-water lakes compared to humic lakes. Our results reveal differences in both community structure and nutritional quality of littoral invertebrates for fish between humic and clear-water lakes.

Persistent Nutritional Deficiency in Pancreatic-Insufficient Children and Adolescents with Cystic Fibrosis Despite Therapeutic Intervention

Abstract Background Cystic fibrosis (CF) is caused by mutations in the gene encoding the CF Transmembrane Conductance Regulator (CFTR) protein, resulting in compromised pulmonary function, malabsorption, and pancreatic insufficiency. The sequelae of CF are heterogeneous, and contributing factors are multifactorial. Nutritional deficiencies, particularly in essential fatty acids (EFA) and vitamins, are known to alter the trajectory of CF unfavorably. The current treatment modalities focus on proactive management of the pulmonary, gastrointestinal, and pancreatic functions; however, information on the monitoring and treatment of nutritional deficiencies in CF patients is scant. The objective of our study was to evaluate the effects of pancreatic function and CFTR modulator therapy on pertinent nutritional deficiencies in children and adolescents diagnosed with CF. Methods The study was approved by our institutional IRB and included 77 non-fasted CF participants, 10.4 + 5.2 years of age (range: 4 months-18 years), and an equal ratio of both sexes. Participants were stratified as pancreatic sufficient (PS, n=6), and pancreatic insufficient with (PI, n=48) or without CFTR modulator treatment (PI-M, n=23). Twenty-two fatty acids were measured in plasma by GC-MS, including omega-6 linoleic acid (LA), and omega-3 α-linoleic acid (ALA). Medical records and 24-hour dietary recalls were reviewed to correlate biochemical and clinical findings. Vitamins A and E and zinc levels of the participants were also obtained. Data analyses were performed by one-way ANOVA and Tukey’s multiple comparisons using Prism software (LaJolla, CA). Results There were no significant differences in BMI and caloric intake among the groups. The concentrations of EFA, LA, and ALA were significantly higher in the PS group (3904±229 nmol/mL and 149±24 nmol/mL) compared to the PI (2387±89 nmol/mL and 53±4 nmol/mL, p&amp;lt;0.01) and PI-M (2335±159 nmol/mL and 58±7 nmol/mL, p&amp;lt;0.01) groups, respectively. Similarly, the biologically active omega-6 arachidonic acid and omega-3 docosahexaenoic acid were lower in the PI and PI-M groups compared to the PS group (p&amp;lt;0.05). Interestingly, EFA deficiency biomarkers, omega-9 mead acid, and the Triene:Tetraene ratio, were significantly higher in the PI group (17±2 nmol/mL and 0.033±0.003) vs PS group (13±3 nmol/mL and 0.019±0.002, p&amp;lt;0.05), respectively. Higher concentrations of vitamins A and E were observed in the PS group vs PI-M groups (p&amp;lt;0.05), and zinc concentrations were not different among the groups. There were no associations between the concentrations of EFA and BMI, age, total caloric intake, and % calories from fat in PS, PI, and PI-M patients. Conclusion While advances in CF therapeutics are remarkable, our data show the persistence of nutritional deficiencies in pancreatic insufficient CF patients regardless of CFTR modulator therapy and support the clinical utility of EFA and vitamins as potential adjuvant biomarkers to assess the severity and assist in the clinical monitoring of CF.

Comparative life cycle assessment of plant and beef-based patties, including carbon opportunity costs

Legume-derived foods have been shown to have comparatively low greenhouse gas (GHG) intensities whilst providing high amounts of nutrients. However, processing legumes into meat analogues can incur significant energy costs. Here, we undertake a comprehensive life cycle assessment of plant-based and (Brazilian and Irish) beef burger patties. Sixteen impact categories are supplemented with the carbon opportunity cost of land occupation, and benchmarked against nutrient density units (NDU) to provide holistic evidence on the potential contribution of plant-based patties to environmentally-sustainable nutritional density. Plant-based patties have a smaller environmental footprint across most categories, including a 77% smaller climate change burden, but incur 8% more energy use compared with Brazilian beef patties. Normalised scores (person equivalents) were significantly larger (p < 0.05) for the beef products across key categories including land use, acidification, and marine and terrestrial eutrophication. Sensitivity analyses indicated significant variance across impact categories if beef cattle are reared in South Africa, France or the United States, including a 16-fold difference in land occupation. Biophysical allocation of co-products reduced environmental burdens of beef burgers. However, owing to a 68% higher NDU per serving, reflecting higher fibre and essential fatty acid content, plant-based patties are associated with 81–87% less climate change and 92–95% less marine eutrophication per NDU compared with beef burger patties. Accounting for carbon opportunity cost of land further increased the climate change advantage of plant-based patties by 25–44%. A simple extrapolation indicates that switching from beef to vegetable patties in the UK could save between 9.5 and 11 million tonnes CO 2 e annually, representing up to 2.4% of territorial GHG emissions.

Particulated Microalgae Composite (PMC) As Substitute For Live Microalgae In Culture Of Juvenile Manila Clam Ruditapes Philippinarum (A. Adams And Reeve, 1850): A Feasibility Analysis

To evaluate the feasibility of particulated microalgae composite (PMC) as live microalgae substitute in artificial breeding of Manila clam Ruditapes philippinarum, effects of replacing Dicrateria inornata with partial or total PMC on the growth, digestive enzyme activities, nutritional ingredient composition and microbial composition in faeces of juvenile clams were investigated. The results showed that 50 and 100% PMC replacement did not affect the mean shell length, mean body weight and survival rate of juvenile clams (p&gt;0.05). Juvenile clams fed with 100% PMC replacement had higher lipase and pepsin activities than those fed with 100% D. inornata (p&lt;0.05), while 50% PMC replacement only increased lipase activity (p&lt;0.05). Essential amino acid (EAA) and polyunsaturated fatty acid (PUFA) contents exhibited no obvious differences between clams fed with and without PMC replacement (p&gt;0.05). Significant increase in delicious amino acid contents (p&lt;0.05) was observed with 100% PMC replacement. PMC replacement did not affect the diversity of dominant phyla in faeces, but the abundance of each dominant phylum. Similar relative abundance of faecal bacteria was observed when D. inornata was replaced with 50 and 100% PMC. PMC appeared to be a potential substitute for microalgae in artificial Manila clam breeding. Keywords: Dicrateria inornata, Microalgae substitute, Particulated microalgae composite, Ruditapes philippinarum