Balance Of Essential Amino Acids Research Articles

Amino acid composition of proteins of hulled and naked oats

The expansion of the production of products with specified functional indicators (cereals, bakery products, pasta, desserts, muesli, yogurts) and feed with a certain amino acid composition is the main task of the food industry and the feed industry, and naked oats have firmly occupied their own niche in these areas of processing. The purpose of the research is to establish the amino acid composition of oat proteins of the hulled oat variety Konkur and naked varieties Bekas and Baget, to assess the balance of essential amino acids in oat proteins relative to the «reference protein» (amino acid scale according to FAO / WHO data) in order to use grain as an additive in food and feed to increase the proportion of essential amino acids in them. The research was carried out during 2018-2022 at the experimental base of the Samara Research Institute of Agriculture. Grain samples of the varieties of filmy and naked oats Konkur, Bekas and Baget were used as experimental material. It was found that the highest proportion of essential amino acids was observed in varieties of naked oats 39,9-41,0 g/kg. The sum of critical amino acids (lysine, methionine, tryptophan) in naked oat varieties was 9,4-9,6 g/kg, which is 1,1-1,3 g/kg higher than in the filmy variety Konkur. According to studies, 1 g of Konkur variety protein contains 249,7 mg of essential amino acids, proteins of naked oat variety Bekas - 268,4 mg and Baget variety - 262,9 mg, which corresponds to 69,4 %, 74,6 % and 73,0 % relative to FAO/WHO recommended standards. The excess of some essential amino acids in naked oat varieties (amino acid score over 100 %) contributed to the imbalance in the ratio of essential amino acids to the «reference» values, while increasing the coefficient of difference in amino acid scores (KRAS protein of Konkur variety was 24,2 %, Bekas variety 33,9 %, varieties Baget 35,5 %), thereby contributing to a decrease in the biological value of the proteins of the varieties Bekas and Baget in relation to the protein of the filmy variety Konkur. Grain of naked oats with a high content of essential amino acids should be used as an ingredient in the production of food and feed with high nutritional value.

Effect of digestible protein on intermediate metabolism, hepatic enzyme activities, energy reserves, and growth performance of pacu (Piaractus mesopotamicus) in the finishing growth phase.

This study investigated the effect of different levels of digestible protein (DP) on blood metabolites, hepatic enzyme activity of glycolysis and amino acid metabolism, energy reserves, and the production characteristics of pacu (Piaractus mesopotamicus) during the finishing growth phase. Six semi purified and isoenergetic diets, containing 16.3, 20.1, 23.8, 27.2, 31.5, and 34.8% of balanced DP, provided with essential amino acid balance, were hand-fed to pacu (1100.0 ± 10.3 g, initial weight) three times daily for 7 weeks. The experiment consisted of six treatments, with three randomly arranged replicates (tanks) per treatment. The data obtained from this experiment were analyzed by one-way analysis of variance (ANOVA), and significant differences (p < 0.05) between treatments were determined using Tukey's test. Blood metabolites, except serum ammonia and the hepatic enzymes activities of glycolysis and amino acid metabolism, except hexokinase activity were affected (p < 0.05) by balanced DP. The energy reserve indices, except hepatic total lipid content, were also found associated (p < 0.05) with balanced DP. The test diets significantly (p < 0.05) affected growth performance parameters. Higher dietary proteins led to a greater energy uptake by fish from the protein in feed. Overall, fish fed the intermediate level (23.8%) of balanced DP with digestible energy of 17.95 MJ kg-1 showed better production traits and physio-biochemical health markers. This information could help nutritionists and farmers to develop nutritionally balanced and economically and environmentally sustainable aquafeed for promoting healthy and sustainable production of pacu in intensive culture systems.

A study on the use of sorrel seed flour (Hibiscus sabdariffa) for improving functionality of wheat flour bread

Bread presents one of the easiest opportunities as a food vehicle for delivery of nutritional and health-promoting benefits to large segments of the world population. However, its low nutritional status due to lack of balance of essential amino acids and inadequate macro- and micronutrients has necessitated recent interest in the development of high-protein hybrid breads (HPHB). Sorrel seed, an underutilized, neglected protein-rich seed holds promising nutritional and antioxidant potentials as source of good quality protein, dietary fibre and bioactive compounds. Furthermore, germination of plant seeds increases the bioavailability of these nutritional and bioactive compounds. Hence, this study has investigated the influence of germination time on nutritional, and functional properties of sorrel seed flour. Further, the amino acid profile, dietary fibre and rheological functionality of wheat-germinated defatted sorrel seed bread were assessed. The sorrel seed was germinated for 24–48 h and defatted. Thereafter, the germinated defatted sorrel seed flours were used to partially replace wheat flour using a linear replacement (w/w) of 95-80% wheat (W) and 5–20% germinated defatted sorrel seed (GS) flours to obtain W95:GS5; W90:GS10, W85:GS15 and W80:GS20. These composite flours and 100% wheat flour (control) were used to produce breads using standard recipe and methods. Results showed significant increase (P < 0.05) in crude protein, dietary fibre and mineral contents after 24 and 48 h germination of sorrel seed. While 24 h germination significantly (P < 0.05) increased WAC from 93.75% to 103.13%, further germination (48 h) caused a reduction of 26.67% (from 93.75 to 68.75%). In vitro protein digestibility of wheat flour decreased significantly (P < 0.05) as supplementation of germinated defatted sorrel seed flour increased. Supplementation of wheat flour with germinated defatted sorrel seed flour in bread production resulted in 51.84–121.42% significant (p < 0.05) increase in the protein content of wheat bread. Similarly, total essential amino acids, dietary fibre, mineral, and ash contents followed the same increasing trend. The in-vivo biological value which ranged from 82.10 to 89.40% was significantly higher (p < 0.05) than 58.30% obtained for the control (100% wheat bread) Thus, inclusion of germinated defatted sorrel seed flour in bread production may serve as a low-cost nutritional supplement for enhancing the nutritional profile and functional benefits of wheat bread.

Amaranth and quinoa as potential nutraceuticals: A review of anti-nutritional factors, health benefits and their applications in food, medicinal and cosmetic sectors.

Amaranth and quinoa are small-seeded grains with high nutritional and phytochemical profiles that promote numerous health benefits and offer protection against various chronic ailments including hypertension, diabetes, cancer, and cardiovascular disorders. They are classified as pseudocereals and possess significant nutritional benefits due to their abundance of proteins, lipids, fiber, vitamins, and minerals. Moreover, they exhibit an exceptional balance of essential amino acids. Despite having several health benefits, these grains have lost their popularity due to their coarse nature and are neglected in developed countries. Research and development activities are growing to explore these underutilized crops, characterizing and valorizing them for food applications. In this context, this review highlights the latest advancements in use of amaranth and quinoa as nutraceutical and functional foods, covering their bioactive substances, anti-nutritional factors, processing techniques, health benefits, and applications. This information will be valuable for planning novel research for efficient use of these neglected grains.

Is the nutritional composition of safflower oilseed meal sufficient for alternative or complementary aqua feeds-raw material?

Safflower (Carthomus tinctorius L.) is grown in many countries, even in arid regions. Due to its important nutrients, safflower has the potential to be used as raw material for the nutrition of many animals and aquaculture species. For this reason, the objective of this study is to determine crude protein, crude oil, ash, and nitrogen-free extract (NFE) values, as well as amino acid, fatty acid, and mineral values, utilizing safflower meal is used instead of fish meal and soybean meal, wheat and canola meal as raw material for aquaculture feed. On a dry matter basis, the crude protein, crude oil, and NFE values were found at 19.42% ±0.32, 8.76% 0.21±, 2.84% 0.1± and 62.68% ±0.88 respectively. Safflower meal contains significant arginine, histidine, and phenylalanine levels, with C18:2 n-6 being one of the most abundant fatty acids. According to the fatty acid values examined, the total saturated fatty acid values were to be 9.79%; the total monounsaturated fatty acid values are 27.58%; total n-6 PUFA values are 61.49%; total n-3 fatty acid values are 0.55% and total n-3 HUFA values are 0.22% in safflower oilseed meal. In terms of potassium and magnesium content, similar to soybean meal and fish meal. Safflower oilseed plant meal or oil can be used as complementary raw material in both marine and freshwater fish feeds. At this point, observing the balance of essential amino acids and polyunsaturated fatty acids in the diets and conducting detailed studies would be effective at further closing the gap in this field.

Amino acid score of the most consumed foods and relationship of the different meal times with the nutritional status of ESPOCH students 2020

Introduction: The quality of food must be diverse, balanced, healthy, which provides energy and nutrients necessary for the perfect functioning of the organism. Objective: To describe the relationship of the main meal times with the nutritional status of university students and to calculate the amino acid score in foods most consumed in the restaurants of the Higher Polytechnic School of Chimborazo-Riobamba-Ecuador. Methodology: Descriptive cross-sectional study with the application of a semi-structured survey on the frequency of eating times for breakfast, lunch, snack and types of preparations with the highest consumption applied to 483 university students who used ESPOCH bars, with voluntary participation. The statistical analysis was carried out with the free version InfoStat/L 2020 program, where it was found that more than 50% are within normal parameters. Results: Regarding the frequency of consumption of the different meal times according to nutritional status, 48.2% had breakfast daily, 59.8% had lunch and 56.3% had a snack. Of the foods with the highest consumption, two limiting amino acids, threonine and tryptophan, are evident due to the consumption of unhealthy foods with a lower biological value of proteins. It is recommended to make suitable combinations to achieve the balance of essential amino acids.

Alternative Protein Sources and Novel Foods: Benefits, Food Applications and Safety Issues.

The increasing size of the human population and the shortage of highly valuable proteinaceous ingredients has prompted the international community to scout for new, sustainable, and natural protein resources from invertebrates (e.g., insects) and underutilized legume crops, unexploited terrestrial and aquatic weeds, and fungi. Insect proteins are known for their nutritional value, being rich in proteins with a good balance of essential amino acids and being a valuable source of essential fatty acids and trace elements. Unconventional legume crops were found rich in nutritional, phytochemical, and therapeutic properties, showing excellent abilities to survive extreme environmental conditions. This review evaluates the recent state of underutilized legume crops, aquatic weeds, fungi, and insects intended as alternative protein sources, from ingredient production to their incorporation in food products, including their food formulations and the functional characteristics of alternative plant-based proteins and edible insect proteins as novel foods. Emphasis is also placed on safety issues due to the presence of anti-nutritional factors and allergenic proteins in insects and/or underutilized legumes. The functional and biological activities of protein hydrolysates from different protein sources are reviewed, along with bioactive peptides displaying antihypertensive, antioxidant, antidiabetic, and/or antimicrobial activity. Due to the healthy properties of these foods for the high abundance of bioactive peptides and phytochemicals, more consumers are expected to turn to vegetarianism or veganism in the future, and the increasing demand for such products will be a challenge for the future.

Теоретичні аспекти доцільності створення безглютенових кексів, збагачених сиром кисломолочним, для закладів ресторанного господарства

In this article, the experience of using gluten-free flour for special-purpose confectionery products for restaurants was analyzed. Fortified cupcakes have high customer appeal and a higher nutritional value. A mix of rice and almond flour as an alternative to wheat flour, and cottage cheese with a fat content of 5% as source of protein were selected. The nutrition value, energy content, and biological value of the chosen raw materials (rice flour, almond flour and cottage cheese) were determined. A recipe for low-fat (less than 2,3 times) cupcakes with high protein content (more than 1,7 times) was created. The possibility of replacing wheat flour with other types of flour, such as rice flour and almond flour, in the ratio 2:1 was confirmed. The possibility of replacing a portion of the butter with cottage cheese in a 1:2:3 ratio was established. In the process of analyzing organoleptic properties, profile-graphics compositions for various properties like color, smell, shape, taste, and texture were made. The result shows that the new cupcakes have high-quality properties; the main difference is 24% due to the fact that the products have different taste, structure, and amount of water when compared to the control sample. Indicators for new cupcakes, such as moisture content (increasing to 5%) were determined. New cupcakes have a reduced fat content (43%)compared to the control sample. As a result of the experiments, the recipe for new fortified cupcakes for restaurants with high customer properties, nutrition, biological value, and low energy value was researched and justified. The new fortified cupcakes have good nutrition value with a high level of essential amino acids, which proves that the balance of essential amino acids is about the physiologically necessary norm.

Antianemic activity of quinoa (Chenopodium quinoa Willd) Collana Negra variety and kanihua (Chenopodium pallidicaule Aellen) Ramis variety seed flour in anemic rats

The Andean grains from the Peruvian Altiplano, quinoa (Chenopodium quinoa Willd) and kanihua (Chenopodium pallidicaule Aellen) have high protein content and an optimal balance of essential amino acids and minerals such as iron (19.8 mg/100 g y 17.6 mg/100 g, respectively). The objective of this research was to evaluate the antianemic activity of extruded flour from quinoa seeds variety Negra Collana and kanihua variety Ramis in anemic Holtzman strain rats. The results of the proximal analysis showed high protein content in quinoa at 22% and kanihua at 16.2%, and the acute toxicity test showed harmlessness up to the dose of 15000 mg/Kg in both flours confirmed with the anatomopathological observation of organs such as liver, stomach, lung, kidneys, and brain. In the evaluation of the antianemic activity, a basal average of 29.3 ± 0.2% of hematocrit was observed in the group of anemic rats treated with quinoa flour, and in twelve weeks, it increased to 53.8 ± 0.3% of hematocrit (p ≤ 0.05). A group of anemic rats treated with kanihua flour had a basal average of 29.5 ± 0.3%, and in twelve weeks, it increased to 51.7 ± 0.3% (p ≤ 0.05). A group of rats without anemia treated with quinoa and kanihua flour showed a basal average of 50.2 ± 0.2% and 49.3 ± 0.3%; in twelve weeks, it increased to 55.2 ± 0.2% and 54.8 ± 0.1%, respectively. It was concluded that oral administration of 360 mg/Kg every 24 h of quinoa flour and kanihua flour increased hematocrit levels by 24.5 ± 0.5% and 22.2 ± 0.3%; weight 65.8 ± 0.3 g and 59.2 ± 0.1 g; height 6.8 ± 0.1 cm and 5.7 ± 0.5 cm, respectively (p ≤ 0.05). In rats without anemia increased hematocrit levels by 5.3 ± 0.0% and 5.5 ± 0.0%; weight 37.7 ± 0.1 g and 21.7 ± 0.05 g; height 4 ± 0.0 cm and 3.9 ± 0.0 cm, respectively (p ≤ 0.05).

Rapeseed meal as a feed component in monogastric animal nutrition – a review

Abstract Rapeseed is an important oil crop worldwide, with an annual production of more than 70 million tons. Rapeseed meal (RSM) is a by-product of rapeseed oil production and is second after soybean meal (SBM) in the world production of protein meal. Rapeseed meal derived from black-seeded winter oilseed rape (Brassica napus L.) usually contains between 35 and 40% of crude protein (CP), which is considered to be one of the more valuable plant proteins. It has a good balance of essential amino acids and a very high protein efficiency ratio (PER=3.29). However, full utilisation of this protein is difficult due to presence of the non-protein components of the seed which are associated with it. These are called antinutritional factors and they limit the utilisation of RSM in monogastric animal nutrition. The main antinutritional factors in RSM are dietary fibre, glucosinolates, phytic acid, and phenolic compounds (sinapine, tannins). For many years, research has been conducted in many centres around the world to improve the nutritional value of RSM, which will consequently increase its use in feeding monogastric animals. The attempts that have been undertaken include breeding strategy, optimisation, modernisation and better control of the oil extraction process, as well as technological treatments of seeds and meal. This review provides information on how RSM has evolved in recent years, as well as on its nutritive value, particularly protein, fibre and glucosinolate content. Techniques which have been used to improve the nutritional value of rapeseed products are also discussed. However, the used methods do not allow for full replacement soybean meal by RSM in monogastric animal nutrition.

Physicochemical and functional properties of Pleurotus geesteranus proteins

Edible mushroom protein has been regarded as a promising protein source due to its nutritional value and sustainability. In the present study, Pleurotus geesteranus proteins were extracted with alkaline solution and then precipitated with salting out (PPS) and isoelectric point precipitation (PPI), respectively. The influences of precipitation method on the physicochemical and functional properties of these two kinds of proteins were studied. The results showed that both PPS and PPI had a good balance of essential amino acids. These two proteins were mainly consisted of polypeptides with a molecular weight lower than 70 kDa. Using proteome analysis, a number of 772 and 459 protein compositions were identified in PPS and PPI, respectively. Compared to PPS, PPI showed a higher zeta potential, higher surface hydrophobicity, lower content of β-sheet and β-turn secondary structure, as well as lower denaturation temperature (Td) and enthalpy change of the denaturation (ΔH). These differences in the physicochemical properties between PPS and PPI resulted in the occurrence of differences in their functional and digestive properties. For example, PPS showed obviously higher protein solubility in water than PPI, especially at natural pH, PPS solution was clear, while PPI showed precipitates. PPI had higher foam capability (FC), lower foaming stability (FS), and lower emulsion stability index (ESI) as compared to PPS. PPI was easier to digest in the pepsin digestion period, while PPS showed a higher nitrogen release after trypsin digestion. These findings on the physicochemical and functional properties of P. geesteranus proteins will help to broaden their applications as protein ingredient in food industry.

Benefits and Challenges of Jatropha Meal as Novel Biofeed for Animal Production.

Jatropha curcas L. has gained importance as a source of seed oil for biodiesel production. The meal contained about 60% protein with a good balance of essential amino acids, containing various bioactive compounds, including saponins, phytic acids, trypsin inhibitors, lectins, phenolics, and flavonoids, which render it as a potential biofeed for animal production. The Jatropha meal demonstrated various biological activities, including antioxidant, antibacterial, and anti-inflammatory effects which enhance its property as a bio-feed. The levels of these bioactive compounds in the seeds are dependent on the genotypes. The J. curcas possessed different varieties which are either toxic or non-toxic according to the presence of phorbol esters. The presence of phorbol esters in the meal confirmed the toxic variety of Jatropha resulting in the limited application of meal as a biofeed. The Jatropha meal devoid of phorbol esters could be applied as a biofeed in the animal production industry, and for the toxic varieties, various techniques such as physicochemical and biological treatments have been introduced to the industry to remove the phorbol esters from Jatropha meal. Several studies employing various cells and animals confirmed the toxicity of the phorbol esters. The molecular mechanism of action of phorbol esters is through up-regulation of PKC-β II gene, overexpression of down-stream proto-oncogenes resulted in inflammation and oxidative stress ending by apoptotic cell death. Despite the presence of valuable bioactive compounds in the Jatropha meal, its nutritional application is not recommended unless the phorbol esters are completely removed.

Study of properties of wheat germins and meals and their use in the production of dietary hardtacks

Object of research: technology of production of dietetic hardtacks using germs and meal of wheat germ, by defatting them.&#x0D; Investigated problem: the process of defatting wheat germ meal not only reduces the total amount of lipids, but also contributes to a change in the ratio of individual fatty acids.&#x0D; Main scientific results: in order to use wheat germ meal as the main raw material, together with wheat germ in the production of dietetic hardtacks, their amino acid composition, biological value and balance of essential amino acids were studied in comparison with the FAO/WHO standard. The mineral, vitamin and fatty acid composition of the germ and meal of wheat germ has been determined. The results obtained were used in the development of the recipe composition and the technological process for the production of dietetic hardtacks.&#x0D; The results obtained (calculation of the balance according to the "tryptophan" and "threonine" indices) confirm that wheat germ meal is a valuable raw material that can be used in technologies for the production of high-protein food products.&#x0D; The moisture-absorbing capacity of wheat germ and meal was studied in order to determine their ability to form dough. It has been established that the best performance is characterized by a mixture of wheat germ meal and wheat germ in a ratio of 80:20.&#x0D; The ability of digestion by enzymes of the gastrointestinal tract "in vitro" of wheat germ meal proteins has been investigated, which determines the biological value of food products and makes it possible to predict the degree of their utilization by the body.&#x0D; The field of practical use of the research results: production of dietetic hardtacks in restaurant enterprises, in specialized confectionery shops, as well as within the boundaries of food production&#x0D; An innovative technological product: the possibility of using valuable secondary raw materials in food production technology – wheat germ and meal, which is a source of high-quality protein, vitamins and minerals. This will make it possible to adjust the nutritional and biological value of flour confectionery products, as well as improve the nutritional structure of consumers of these products.&#x0D; Scope of application of an innovative technological product: dietetic hardtacks

Correction of imbalance of essentialaminoacids of blood plasma in Patients with stableangina

Introduction. Ischemic heart disease is the leading nosology calunit in the structure of cardiovascular diseases interms of disability and mortality among the population of Ukraine. The purpose. To improve the treatment of patients with stable angina by studying the effect of L-arginine on the balance of essential amino acids in blood plasma. Material and methods. It was examined 85 patients with stable angina. They were divided into two groups: group Ipatients received antianginal basic therapy, group II patients received basic antianginal therapy and L-arginine. The amino acid spectrum of patients' blood plasma was studied by ion-exchange liquid column chromatography, using an automatic amino acid analyzer T-339 Microtechna (Czech Republic, Prague). Results and discussion. In patients with stable angina who received basic therapy and L-arginine, in contrast to patients who received only basic therapy, plasma levels of arginine became normalized, which probably contributes to the synthesis of NO. The level of valine, leucine and isoleucine, which provide the synthesis of acyl-CoA and succinyl-CoA, became also normalized.Conclusion. Administration of L-arginine to patients with stable angina together with antianginal therapy helps to correct plasma amino acid imbalances, which is likely to effectively affect the course of the disease and prognosis.

Physicochemical and emulsifying properties of protein isolated from Phlebopus portentosus

Edible mushroom proteins have attracted increasing interest due to their high content and nutritional quality. In the present work, Phlebopus portentosus protein isolate (PPI) was extracted from P. portentosus fruiting bodies using alkali extraction and isoelectric precipitation method, and the physicochemical and emulsifying properties of PPI were systematically investigated. PPI had a good balance of essential amino acids, but showed low solubility in water. Therefore, a protein nanoparticulation strategy was used to prepare stable PPI nanoparticle (PPIP) dispersions. PPIP showed an average diameter of 225.91 nm, an oil-in-water three-phase contact angle (θo/w) of 95.62°, and fast adsorption behavior at the oil-water interfaces. The near-neutral wettability and high interfacial ability facilitate PPIP as a single emulsifier to fabricate a double Pickering emulsion via the one-step homogenization. The properties of PPIP-stabilized double emulsions were dependent on the protein concentration and oil content. For example, an increase in protein concentration resulted in the decrease of small internal droplet number, droplet size, and storage modulus; an increase in oil content also led to the decrease in the droplet size. These results suggest that PPI has a great potential to be used as emulsifiers for preparing double emulsions.

Comparison of natural curd whey and its nanoconcentrate in regard to the nutritional and biological value

The article is devoted to studying the effect of membrane filtration on the change in the biological value of natural curd whey and its nanoconcentrate. As the statistics shows, no more than 20% of natural curd whey is subjected to deep processing. Some techniques for increasing the dry matter concentration have been considered, and their disadvantages have been identified. The most up-to-date technique–nanofiltration–has been chosen, the main advantages of which are high efficiency of concentration and partial demineralization effect during whey processing. The purpose of the work is to study the effect of nanofiltration treatment on the change in the amino acid composition of natural curd whey and nanoconcentrate. The work studies the natural curd whey received in the process of low-fat. Experimental data showing the essential amino acids content have been obtained. The amino acids content was determined by gas chromatography. The calculation of the amino acid score in natural curd whey and its nanoconcentrate has been done. Limiting amino acids have been found both in natural curd whey and nanococentrate. The values of comparable redundancy index, the essential amino acids index, and the utility coefficient were calculated and compared. Using the obtained calculations, it is possible to conclude that the nanoconcentrate in comparison with natural curd whey has a higher balance of essential amino acids, their number increases, and it is necessary to take these data into account when developing new types of products having increased biological value.

The effect of L-arginine on the balance of essential amino acids in plasma of the patients with stable angina

Background. Despite the significant achievements of clinical medicine in the prevention, diagnosis and treatment of coronary heart disease, the levels of morbidity, disability and mortality among the population of Ukraine from this pathology remain consistently high. The purpose. To improve the treatment of patients with stable angina by studying the effect of L-arginine on the balance of essential amino acids in blood plasma. Material and methods. It was examined 67 patients with stable angina. They were divided into two groups: group Ipatients received antianginal basic therapy, group II patients received basic antianginal therapy and L-arginine. The amino acid spectrum of patients' blood plasma was studied by ion-exchange liquid column chromatography, using an automatic amino acid analyzer T-339 Microtechna (Czech Republic, Prague). Results and discussion. In patients with stable angina who received basic therapy and L-arginine, in contrast to patients who received only basic therapy, plasma levels of arginine became normalized, which probably contributes to the synthesis of NO. The level of valine, leucine and isoleucine, which provide the synthesis of acyl-CoA and succinyl-CoA, became also normalized. Conclusion. Administration of L-arginine to patients with stable angina together with antianginal therapy helps to correct plasma amino acid imbalances, which is likely to effectively affect the course of the disease and prognosis.

Quality of gluten-free cookies made with rice flour of different levels of amylose and cowpea beans

PurposeThe objective of this study was to evaluate the effects of rice flour obtained from rice grains with different levels of amylose on technological, nutritional and sensory properties of cookies made with a blend of rice and cowpea flour.Design/methodology/approachThe cookies preparation was set at a ratio of rice flour and cowpea beans 70:30. The studied formulations were: LA: low amylose rice flour; MA: medium-amylose rice flour; HA: high amylose rice flour. The quality of the obtained cookies was analyzed for proximate composition, in vitro protein digestibility, thickness, diameter, dispersion factor, texture, color, amino acid profile and sensory properties.FindingsProximate composition and in vitro protein digestibility showed no differences between the three studied formulations. The medium and low amylose rice flour cookies showed the lowest hardness values. The combination of rice and beans allowed a good balance of essential amino acids. The cookies formulated with high amylose rice flour presented lighter coloration, low hardness and greater sensory preference.Originality/valueRice and cowpea flours are an alternative source for the preparation of gluten-free bakery products, such as cookies. The high amylose content of rice flour has less negative interference in the texture characteristics of the cookies. The combination of rice and beans flour provides a balance of essential amino acids.

Physicochemical properties and acceptability of three formulations containing fava bean, quinoa and corn flour extrudates

Andean grains, widely distributed in the high areas of the Andean Highlands, can help to reduce malnutrition owing to their high content of protein and balance of essential amino acids. Recently, their consumption has changed as they are increasingly used for instant food mixtures. However, a better design and formulation are necessary to maintain Andean grains’ nutritional value and characteristics. In this study, we evaluated the physicochemical properties and acceptability of three formulations containing extruded corn (Zea mays L.), fava bean (Vicia faba L.), and quinoa (Chenopodium quinoa Willd) flours. Grain samples were collected from Choclococha, Acobamba, Huancavelica, Peru, and extruded at 95°C. Subsequently, three flour formulations were prepared. The formulation comprising 30% fava bean flour, 58% quinoa flour, and 12% corn flour consisted of 18.64% protein, 4.87% fat, 8.4% humidity, 2.99% ash, and 62.44% carbohydrates. This formulation with the highest quinoa flour content presented the highest acceptability in terms of color odor and flavor, and the highest protein content and digestibility (72.6%) of all analyzed formulations. Thermal analysis and pasting testing indicated that the formulation with the highest acceptability did not contain native starch; moreover, the proteins in this formulation were denatured.

Teff (Eragrostis tef) processing, utilization and future opportunities: a review

AbstractThe demand for food production has been constantly increasing due to rising population. In developed countries, for example, the emergence of regional production of old grains that are rarely utilized, along with the production of commonly consumed grains, has gained importance in recent years. These grains, known collectively as ancient or heirloom grains, have offered both farmers and consumers novel ways of cultivation and products with interesting taste, characteristics and nutritional value. Among the 30 000 plant species known, only five cereals currently provide more than 50% of the world's energy intake – bread wheat (Triticum aestivum), rice (Oryza sativa), sorghum (Sorghum bicolor), millets (Panicum sp.) and maize (Zea mays). The excessive utilization of these selected species has a great potential to cause genetic losses and difficulty in bridging future agricultural demands. Teff (Eragrostis tef), an ancient grain extensively cultivated in countries like Eritrea and Ethiopia, provides promising alternatives for new food uses since its nutritional value is significantly higher than most others cereal grains. The absence of gluten allows flexibility in food utilization since it can be directly substituted to gluten‐containing products. The grain also offers an excellent balance of essential amino acids and minerals, which can fulfil the recommended daily intake and eliminates the need for fortification and enrichment. This review provides a general overview of the physical properties and nutritional composition of teff grains related to processing and applications in the food and feed industries. The current status of teff utilization, as well as the challenges in production and commercialization, and future opportunities is presented and discussed.