Essential Activity Research Articles

Therapeutic targeting ERRγ suppresses metastasis via extracellular matrix remodeling in small cell lung cancer

Small-cell lung cancer (SCLC) is the most aggressive and lethal type of lung cancer, characterized by limited treatment options, early and frequent metastasis. However, the determinants of metastasis in SCLC are poorly defined. Here, we show that estrogen-related receptor gamma (ERRγ) is overexpressed in metastatic SCLC tumors, and is positively associated with SCLC progression. ERRγ functions as an essential activator of extracellular matrix (ECM) remodeling and cell adhesion, two critical steps in metastasis, by directly regulating the expression of major genes involved in these processes. Genetic and pharmacological inhibition of ERRγ markedly reduces collagen production, cell-matrix adhesion, microfilament production, and eventually blocks SCLC cell invasion and tumor metastasis. Notably, ERRγ antagonists significantly suppressed tumor growth and metastasis and restored SCLC vulnerability to chemotherapy in multiple cell-derived and patient-derived xenograft models. Taken together, these findings establish ERRγ as an attractive target for metastatic SCLC and provide a potential pharmacological strategy for treating this lethal disease.

An Improve Method for Plant Leaf Disease Detection and Classification using Deep Learning

In countries like India, whose important occupation is agriculture, face a huge loss when the crops get affected by any type of disease. These diseases attack the crops in various stages and can destroy the entire production. Since most diseases are transmitted from one crop to another there is an essential requirement to detect the type of disease in the early stage so that farmers can take the required action to “save the crops” and production. Early disease detection is one of the essential activities for enhancing agricultural productivity. Diseases spread very quickly in the parts of the leaves that affect the growth of the plants. Early detection is a challenging task as the symptoms are mild for accurate identification. This research paper presents an enhanced CNN based MCC-ECNN model with fine-tuned hyper-parameters and various batch sizes for accurate plant leaf disease classification.

15-minute cities, ‘walkability’ and last millimeter problems

The 15-minute city concept is about planning and building cities so that most essential activities can be accessed within 15-minutes of a residence on foot or by bicycle. The possibilities and limitations of the concept for disabled persons have yet to be fully considered. Here I centre disability in a hopeful critique of concepts central to 15-minute city implementation. I argue that greater attention should be given to what I call last millimeter problems, barriers within small spaces (e.g. subway platform gaps) disabled persons face in transportation systems and the built environment that can make any city, including a 15-minute city, an impassable city.

Strategic Analysis from a consulting context for the Super Kinder School Institution

In the current dynamic, managers of educational institutions such as Super Kinder School seek correct decisions to enhance internal efficiency and meet organizational objectives. A strategic analysis was carried out applying the interpretivist methodology through a case design and an interview with the director of the institution. This qualitative approach has given rise to strategic matrices such as Porter's Five Forces, EFE, EFI, IE, SWOT, PEYEA, MPCE, MIC MAC, complemented by the Balanced Scorecard tool. Strategies focused on market growth, product and service development, and market penetration were identified, seeking to improve internal processes and promote the growth of Super Kinder School. The consultancy is aimed at addressing essential activities for the continuous development of Super Kinder School, requiring a deep understanding of the institution, its achievements and educational service

Strategic Analysis from a consulting context for the Super Kinder School Institution

In the current dynamic, managers of educational institutions such as Super Kinder School seek correct decisions to enhance internal efficiency and meet organizational objectives. A strategic analysis was carried out applying the interpretivist methodology through a case design and an interview with the director of the institution. This qualitative approach has given rise to strategic matrices such as Porter's Five Forces, EFE, EFI, IE, SWOT, PEYEA, MPCE, MIC MAC, complemented by the Balanced Scorecard tool. Strategies focused on market growth, product and service development, and market penetration were identified, seeking to improve internal processes and promote the growth of Super Kinder School. The consultancy is aimed at addressing essential activities for the continuous development of Super Kinder School, requiring a deep understanding of the institution, its achievements and educational service

Essential Oil Content, Composition and Free Radical Scavenging Activity from Different Plant Parts of Wild Sea Fennel (Crithmum maritimum L.) in Montenegro.

This study was conducted to determine the sea fennel essential oil (SFEO) yield, composition, and antioxidant activity of leaves, stem, inflorescences, and umbels from seeds of wild sea fennel (SF) (Crithmum maritimum L.) from the Montenegro coast. The chemical composition of isolated essential oil was determined by GC/MS and GC/FID analyses. The antioxidant activity was determined using the DPPH assay. The maximum SFEO yield was found in umbels with seeds (4.77 mL/100 g p.m.). The leaves contained less EO (0.52 mL/100 g p.m.) than immature inflorescence (0.83 mL/100 g p.m.) The minimum EO content was found in the stem (0.08%). Twenty components were isolated from SFEO leaves, twenty-four from inflorescence, thirty-four components from the stem, and twenty-one components from umbels with seeds. Limonene (62.4-72.0%), γ-terpinene (9.5-14.0%), α-pinene (1.4-5.8%), and sabinene (1-6.5%) were found to be the main components of the SFEO from monoterpene hydrocarbons as dominant grouped components (86% to 98.1%). SF plant parts showed differences in chemical profiles, especially in specific and low-represented ingredients. (E)-anethole (4.4%), fenchone (0.5%), and trans-carveol (0.2%) were present only in umbel with seeds, while the β-longipipene (0.5%), (E)-caryophyllene (0.5%), and (2E)-decenal (0.2%) were found only in the stems. The degree of DPPH radical neutralization increased with incubation time. The SFEO isolated from the stems showed stronger antioxidant activity during the incubation times of 20 and 40 min (EC50 value of 5.30 mg/mL and 5.04 mg/mL, respectively) in comparison to the SFEO isolated from the other plant parts. The lowest antioxidant activity was obtained with the SFEO leaves (155.25 mg/mL and 58.30 mg/mL, respectively). This study indicates that SFEO possesses significant antioxidant activities and is animportant component in the food and pharmaceutical industries. It is important to preserve the existing gene pool and biodiversity with rational use SF for the extraction of high-quality essential oils.

Fishing Eco-Efficiency of Ports in Northwest Spain

Fishing is an essential economic activity and source of livelihood for millions of people worldwide. However, overfishing and unsustainable practices have led to a decline in fish populations and the degradation of marine ecosystems. Moreover, fishing activities can contribute to climate change through the emission of greenhouse gases (e.g., carbon dioxide and methane) from fishing vessels and seafood transportation. To mitigate the environmental impacts of fishing activities, sustainable fishing practices must be implemented to minimize the negative impacts of fishing on the environment while maintaining the productivity and diversity of fish populations and ecosystems. These practices include using selective fishing gear, avoiding fishing in vulnerable habitats, implementing fishery management plans, and reducing the carbon footprint of the fishing industry. To this end, and as a first step in defining efficient and effective measures towards the sustainability of capture fishing activity, an analysis of the environmental sustainability of the Galician fishing sector, one of the main European regions in this field, is presented in this work. An ecosystem-based indicator (ecological footprint, calculated by adding the so-called fishing ground footprint and the carbon footprint) was employed to quantify the main impacts of capture fishing during extractive activity. The catch composition and fuel consumption of the fleet based on the vessels’ power, and economic benefits, were the parameters used in this analysis. The results showed that ports with larger vessels and fleets seem to be more eco-efficient than those concentrating smaller vessels in targeting lower trophic level species.

Treatment paths and resources used for patients with a left ventricular assist device: an Italian survey

Abstract Background The number of people with end-stage Heart Failure implanted with a Left Ventricular Assist Device (LVAD) is constantly increasing, demanding hospitals to develop new and specific treatment paths. No previous studies described services and treatments available for these patients at national level. Knowing how hospitals organize their resources in order to meet patients’ need would be useful to standardize and improve care offered to this population. Purpose To investigate how Italian hospitals that are reference centers for people with LVAD organize treatment paths and resources for these patients. Methods A cross-sectional observational study was conducted. A survey including questions about all the relevant aspects of treatment paths for people with LVAD was created and validated by 4 LVAD experts (2 nurses and 2 physicians) from 2 different hospitals. The survey was then administered to the 22 (> 95%) of the Italian hospitals that are reference centers for people with LVAD. Results Results show a picture of how treatment paths and resources for people with LVAD are organized in Italy nowadays. Nurses play a pivotal role in the multi-professional team that takes care of these patients, being often acknowledged as LVAD Coordinator (case-manager) and being involved in crucial activities such as health education, monitoring of health conditions before and after dismission, and improving patients’ empowerment. Health education is recognized as an essential activity, but many hospitals still struggle to organize it according to patients’ needs and not organizational ones. Palliative care, even if recognized by the most recent guidelines as a crucial element of treatment options in people with LVAD, is a need that remains often unmet. Conclusion The growing number of people with LVAD requires an effort for re-organizing resources and treatment paths for them. There is a great need for integrating palliative care in the several treatment options for these patients. Nurses play a pivotal role in all stages of the treatment paths, and should be provided with specific education, as the whole multi-professional team.

Analisis Kebutuhan Buku Cerita Bergambar sebagai Pengantar Bermain di Taman Kanak-Kanak

The availability of picture storybooks is a crucial component of early childhood education. This research aims to describe and analyze the needs for storybooks among Kindergarten students through a questionnaire. At an early age, play is an essential activity that supports children's cognitive, social, emotional, and motor development. The storybooks used in this process must have specific characteristics that captivate children's interest while aligning with educational objectives. The research methods employed include observations, interviews with kindergarten teachers, and content analysis of popular storybooks among young children. The findings are intended to serve as a foundation for developing educational materials, particularly picture storybooks that facilitate play-based learning for kindergarten students. The ultimate goal is to enhance the quality of educational resources available to young learners, ensuring they are both engaging and educationally beneficial.

Chondroitin Sulfate Derivative Cross-Linking of Decellularized Heart Valve for the Improvement of Mechanical Properties, Hemocompatibility, and Endothelialization.

Tissue-engineered heart valve (TEHV) has emerged as a prospective alternative to conventional valve prostheses. The decellularized heart valve (DHV) represents a promising TEHV scaffold that preserves the natural three-dimensional structure and retains essential biological activity. However, the limited mechanical strength, fast degradation, poor hemocompatibility, and lack of endothelialization of DHV restrict its clinical use, which is necessary for ensuring its long-term durability. Herein, we used oxidized chondroitin sulfate (ChS), one of the main components of the extracellular matrix with various biological activities, to cross-link DHV to overcome the above problems. In addition, the ChS-adipic dihydrazide was used to react with residual aldehyde groups, thus preventing potential calcification. The results indicated notable enhancements in mechanical properties and resilience against elastase and collagenase degradation in vitro as well as the ability to withstand extended periods of storage without compromising the structural integrity of valve scaffolds. Additionally, the newly cross-linked valves exhibited favorable hemocompatibility in vitro and in vivo, thereby demonstrating exceptional biocompatibility. Furthermore, the scaffolds exhibited traits of gradual degradation and resistance to calcification through a rat subcutaneous implantation model. In the rat abdominal aorta implantation model, the scaffolds demonstrated favorable endothelialization, commendable patency, and a diminished pro-inflammatory response. As a result, the newly constructed DHV scaffold offers a compelling alternative to traditional valve prostheses, which potentially advances the field of TEHV.

Environmental Factors Related to Climate Change Alter the Chemical Composition and Biological Activity of Lavandula viridis L’Hér Essential Oil

Climate change is affecting all regions of the world, and the Mediterranean region is one of the most affected. Plants accumulate secondary metabolites as an adaptive response to stress circumstances. The present study investigated the effect of different abiotic factor conditions (drought, moderate heat, severe heat, salinity, and UV-B radiation) on the essential oil (EO) yield, composition (volatile profile), and biological activity (enzyme inhibition and antioxidant activity) of Lavandula viridis L’Hér. In general, the environmental conditions increased the extraction yield of EO. Eighty-two compounds were identified in the EO and environmental factors induced some quantitative changes in EO composition. Severe heat and salinity conditions increased the concentration of the two most abundant compounds, 1,8-cineole and camphor. Severe heat also increased the potential of EO to inhibit the enzymes butyrylcholinesterase and tyrosinase. Drought, salinity, and UV-B radiation promoted the ability of EO to inhibit acetylcholinesterase. In addition, heat and drought enhanced the antioxidant activity of EO. These results are relevant for exploring the potential of this EO for industrial applications, although future studies combining the factors studied are important to understand the influence of synergistic effects on the composition and bioactivity of the plant products obtained.

Effectiveness of Earplugs and Eye Masks on Sleep Quality and Fatigue Among Nonventilated Patients in an Intensive Care Unit.

Sleep is a basic physiological need and is imperative for healing and rejuvenation. However, the environment of the intensive care unit (ICU), including loud sounds and bright lights, can undermine patients' sleep quality. This study was designed to determine the effectiveness of overnight use of earplugs and eye masks to improve sleep quality and potentially influence hemodynamic parameters and mitigate fatigue among nonventilated patients in an ICU. This experimental study was conducted among 84 nonventilated patients in an ICU. The patients were evenly divided between an intervention group (n=42) and a control group (n=42). The intervention group patients received earplugs and eye masks for three consecutive nights from 10:30 pm to 6:30 am, while the control group patients received routine care. Clinical data, responses to the Richards-Campbell sleep questionnaire, and rankings on a numerical fatigue scale were collected before (pretest) and after the intervention each night and the next morning (posttests 1, 2, and 3). The results revealed a significant reduction in fatigue. At pretest, fatigue scores in the study group were 4.19±1.64. The score was significantly reduced to 3.40±1.39 at posttest 1 and then to 2.21±1.00 at posttest 3 (p<0.0001). The sleep quality for the study group showed a significant improvement from the pretest score of 43.73±8.27 to 60.35±6.85 at posttest 3 (p<0.001), whereas the control group had slightly worse sleep quality, with 40.64±8.67 at pretest and 45.63±6.95 at posttest 3. Continuous patient monitoring is an essential nursing care activity in ICUs while ensuring good-quality sleep promotes healing and reduces fatigue. Sleep quality can be supported by devices such as earplugs and eye masks to limit undue disturbances in the ICU settings.

Doctor-patient Communication in Healthcare: A Scoping Review

Communication is a vital component in healthcare system. One of life's most essential activities is the ability to convey information. This transmission of information between an informer and a recipient is known as communication. For an effective communication, nature of the information to be shared, and engagement with technology; effective and efficient use of information and technology play a crucial role. As healthcare professional has privilege of meeting, treating and caring for individuals during what may be the most vulnerable period of their lives. For communication to be effective the message needs to be thoughtfully and clearly delivered wherein one should have the ability to express the technical words in a layman language to enhance patients understanding, cooperation, and satisfaction. A communication can be Verbal, Non-verbal, Formal, Informal, Technology aided and through signs and symbols. Communication is a tool which satisfy’s patient’s mental, psychological deal and enhances the effectiveness of the intervention along with adherence to the treatment protocol. Mode to communicate with patients varies as per their anxiety, need, severity and psycho-social understanding of the each patient.

Clinical Evidence on Expansion of Essential Oil-Based Formulation's Pharmacological Activity in Bovine Mastitis Treatment: Antifungal Potential as Added Value.

Bovine mastitis, as a significant and prevalent health problem in dairy herds, is primarily attributed to bacterial infections. Yeasts, although considered relatively rare causative agents, have also been associated with mastitis in dairy cattle. Current mastitis treatment predominantly relies on antibiotics, with limited emphasis on antifungal treatment. However, mycotic mastitis is challenging to treat, since these fungi are often resistant to antibiotics and may even utilize them for energy. In the current research, the in vivo antimicrobial activity of the essential oil-based formulation (Phyto-Bomat), as a possible alternative mastitis treatment associated with yeasts, was studied. This study involved a total of 68 animals from two dairy farms with diagnosed mastitis, and three treatment groups were established: conventional antibiotic treatment, Phyto-Bomat treatment, and the combination of both. The findings suggest significant variations in the presence of Candida samples based on the treatment administered, and the most significant difference was noted in cows treated with the combination (Phyto-Bomat and antibiotics). Yet, it is important to note that the results reveal that, regardless of the treatment type, there are statistically significant differences in the presence of Candida samples across the examined time points. These results aim to provide valuable insights into the potential of EOs as an alternative therapy in bovine mastitis, specifically targeting yeasts. Such findings could offer new strategies in the mycotic mastitis control and reducing the occurrence of secondary infections following antibiotic treatment.

Wild red foxes (Vulpes vulpes) do not participate in SARS-CoV-2 circulation in Poland

Background: Biomonitoring is an essential activity for identifying possible vectors and reservoirs of pathogens and predicting potential outbreaks. Wild red foxes are present in both sylvatic and synanthropic environments, making them potential carriers of zoonotic pathogens. Experimental studies have shown that both coyotes and red foxes can transmit SARS-CoV-2. This study aimed to assess the prevalence and seroprevalence of SARS-CoV-2 in wild red foxes hunted in northern Poland. Methods: Oral swabs, blood clots or heat tissue samples were collected from 292 red foxes hunted in northern Poland. We used both molecular (RT-PCR) and serological (IFA) approaches to detect SARS-CoV-2 infections in the sampled animals. Results: We did not find any evidence of SARS-CoV-2 infection in the collected samples, using both molecular and serological methods. Conclusions: Despite foxes having frequent contact with humans, human waste, and other animals, they do not appear to participate in the circulation of the SARS-CoV-2 virus in our geographical region. Nevertheless, we believe that continuous biomonitoring should be performed to assess the SARS-CoV-2 epidemiological situation in the wild.

A long-term growth stable Halomonas sp. deleted with multiple transposases guided by its metabolic network model Halo-ecGEM

Microbial instability is a common problem during bio-production based on microbial hosts. Halomonas bluephagenesis has been developed as a chassis for next generation industrial biotechnology (NGIB) under open and unsterile conditions. However, the hidden genomic information and peculiar metabolism have significantly hampered its deep exploitation for cell-factory engineering. Based on the freshly completed genome sequence of H. bluephagenesis TD01, which reveals 1889 biological process-associated genes grouped into 84 GO-slim terms. An enzyme constrained genome-scale metabolic model Halo-ecGEM was constructed, which showed strong ability to simulate fed-batch fermentations. A visible salt-stress responsive landscape was achieved by combining GO-slim term enrichment and CVT-based omics profiling, demonstrating that cells deploy most of the protein resources by force to support the essential activity of translation and protein metabolism when exposed to salt stress. Under the guidance of Halo-ecGEM, eight transposases were deleted, leading to a significantly enhanced stability for its growth and bioproduction of various polyhydroxyalkanoates (PHA) including 3-hydroxybutyrate (3HB) homopolymer PHB, 3HB and 3-hydroxyvalerate (3HV) copolymer PHBV, as well as 3HB and 4-hydroxyvalerate (4HB) copolymer P34HB. This study sheds new light on the metabolic characteristics and stress-response landscape of H. bluephagenesis, achieving for the first time to construct a long-term growth stable chassis for industrial applications. For the first time, it was demonstrated that genome encoded transposons are the reason for microbial instability during growth in flasks and fermentors.

QGeoWEPP: An open-source geospatial interface to enable high-resolution watershed-based soil erosion assessment

Predicting soil erosion by water is an essential natural resource management activity. The Water Erosion Prediction Project (WEPP) model is a process-based continuous simulation tool based on erosion mechanics and channel hydraulics. Due to the extensive data requirements, preparing input parameter settings for WEPP can be time-consuming. A Geospatial Interface for WEPP (GeoWEPP) overcame this major disadvantage but had limitations regarding keeping track of operating systems and proprietary software updates. QGeoWEPP is a newly developed open-source QGIS-based GeoWEPP, offering additional novel user-based customizations of model simulations including validation data sets. QGeoWEPP provides a platform for applying WEPP at the hillslope and watershed scales that integrates the whole model application and validation process. QGeoWEPP allows applying WEPP in any study area worldwide with minimum data limitations and more spatial and temporal capabilities in areas such as soil and water conservation, land management, geohazard assessment due to land use and climate change, and many more.

Divergent evolution of sleep in Drosophila species

Living organisms synchronize their biological activities with the earth’s rotation through the circadian clock, a molecular mechanism that regulates biology and behavior daily. This synchronization factually maximizes positive activities (e.g., social interactions, feeding) during safe periods, and minimizes exposure to dangers (e.g., predation, darkness) typically at night. Beyond basic circadian regulation, some behaviors like sleep have an additional layer of homeostatic control, ensuring those essential activities are fulfilled. While sleep is predominantly governed by the circadian clock, a secondary homeostatic regulator, though not well-understood, ensures adherence to necessary sleep amounts and hints at a fundamental biological function of sleep beyond simple energy conservation and safety. Here we explore sleep regulation across seven Drosophila species with diverse ecological niches, revealing that while circadian-driven sleep aspects are consistent, homeostatic regulation varies significantly. The findings suggest that in Drosophilids, sleep evolved primarily for circadian purposes. The more complex, homeostatically regulated functions of sleep appear to have evolved independently in a species-specific manner, and are not universally conserved. This laboratory model may reproduce and recapitulate primordial sleep evolution.

Generating Hidden Markov Models from Process Models Through Nonnegative Tensor Factorization

Monitoring of industrial processes is a critical capability in industry and in government to ensure reliability of production cycles, quick emergency response, and national security. Process monitoring allows users to gauge the progress of an organization in an industrial process or predict the degradation or aging of machine parts in processes taking place at a remote location. Similar to many data science applications, we usually only have access to limited raw data, such as satellite imagery, short video clips, event logs, and signatures captured by a small set of sensors. To combat data scarcity, we leverage the knowledge of Subject Matter Experts (SMEs) who are familiar with the actions of interest. SMEs provide expert knowledge of the essential activities required for task completion and the resources necessary to carry out each of these activities. Various process mining techniques have been developed for this type of analysis; typically such approaches combine theoretical process models built based on domain expert insights with ad-hoc integration of available pieces of raw data. Here, we introduce a novel mathematically sound method that integrates theoretical process models (as proposed by SMEs) with interrelated minimal Hidden Markov Models (HMM), built via nonnegative tensor factorization. Our method consolidates: (a) theoretical process models, (b) HMMs, (c) coupled nonnegative matrix-tensor factorizations, and (d) custom model selection. To demonstrate our methodology and its abilities, we apply it on simple synthetic and real world process models.

Both IRAK3 and IRAK1 Activate the MyD88-TRAF6 Pathway in Zebrafish.

IL-1R-associated kinases (IRAKs) are signal transducers of the TLR/IL-1R-MyD88-TRAF6 pathways. Vertebrates possess two IRAK lineages, IRAK1/2/3 and IRAK4. In mammals, IRAK4/IRAK1 and IRAK4/IRAK2 are pathway enhancers, whereas IRAK3 is a repressor. However, in bony fish, IRAK2 is absent, and it remains elusive how fish IRAK1/3/4 functionally differ from their mammalian counterparts. In this study, we explored this using the zebrafish model. First, we showed that in human 293T cells, zebrafish IRAK1 and IRAK4 were components of the Myddosome (MyD88-IRAK4-IRAK1) complex, with IRAK1 serving as a potent pathway enhancer. Then, we discovered two zebrafish IRAK3 variants: one (IRAK3a) contains an N-terminal Death domain, a middle pseudokinase domain, and a C-terminal TRAF6-binding domain, whereas the other (IRAK3b) lost both the kinase and TRAF6-binding domains. This truncation of IRAK3 variants could be a conserved phenomenon in fish, because it is also observed in trout and grass carp. We proceeded to show that zebrafish IRAK3a acts as a pathway enhancer by binding with MyD88 and TRAF6, but its activity is milder than IRAK1, possibly because it has no kinase activity. Zebrafish IRAK3b, however, plays a sheer negative role, apparently because of its lack of kinase and TRAF6-binding domains. Moreover, zebrafish IRAK3a/3b inhibit the activity of IRAK1/4, not by interacting with IRAK1/4 but possibly by competing for MyD88 and TRAF6. Finally, we have verified the essential activities of zebrafish IRAK1/3a/3b/4 in zebrafish cells and embryos. In summary, to our knowledge, our findings provide new insights into the molecular functions of fish IRAKs and the evolution of the IRAK functional modes in vertebrates.