Erythrocyte Indices Research Articles

Prevalence Of Suspected Beta Thalassemia Minor Based On Screening With Mentzer Index And Red Distribtion Width In Students Of Smp Negeri 7 Pujut

Background : Beta thalassemia is blood disorder that it often found in Indonesia with high frequency of beta thalassemia carrier genes around 3 to 10%. Thalassemia has not been cured, but can be prevented by screening of thalassemia to identify individuals with beta minor thalassemia in population. The purpose of this study was to determine prevalence of anemia and prevalence of suspected beta thalassemia minor in students of SMP Negeri 7 Pujut based on Mentzer index and Red Distribution Width index.
 Methode: This study was an quantitative descriptive design with cross sectional approach. Total number of 130 students of SMP Negeri 7 Pujut were recruited with proportional random sampling stratified technique. Screening of beta thalassemia minor by calculating erythrocyte index of anemia blood samples using Mentzer index and Red Distribution Width index. Mentzer index was calculated by dividing Mean Corpuscular Volume (MCV) by Red Blood Count (RBC). RDW index was calculated by by multiplying MCV by RDW and divided by RBC.
 Result: According to data analysis, prevalence of anemia in students was 16.9% based on Hb concentrations for diagnosis anaemia based on WHO. Prevalence of suspected beta thalassemia minor was 0% based on Mentzer index and 4,6% based on RDW index.
 Conclusion: Prevalence of anemia in students was 16.9% based on Hb concentrations for diagnosis of anaemia WHO and prevalence of suspected beta thalassemia minor was 4,6% based on RDW index.

Anemia and iron metabolism in COVID-19: a systematic review and meta-analysis

Iron metabolism and anemia may play an important role in multiple organ dysfunction syndrome in Coronavirus disease 2019 (COVID-19). We conducted a systematic review and meta-analysis to evaluate biomarkers of anemia and iron metabolism (hemoglobin, ferritin, transferrin, soluble transferrin receptor, hepcidin, haptoglobin, unsaturated iron-binding capacity, erythropoietin, free erythrocyte protoporphyrine, and erythrocyte indices) in patients diagnosed with COVID-19, and explored their prognostic value. Six bibliographic databases were searched up to August 3rd 2020. We included 189 unique studies, with data from 57,563 COVID-19 patients. Pooled mean hemoglobin and ferritin levels in COVID-19 patients across all ages were 129.7 g/L (95% Confidence Interval (CI), 128.51; 130.88) and 777.33 ng/mL (95% CI, 701.33; 852.77), respectively. Hemoglobin levels were lower with older age, higher percentage of subjects with diabetes, hypertension and overall comorbidities, and admitted to intensive care. Ferritin level increased with older age, increasing proportion of hypertensive study participants, and increasing proportion of mortality. Compared to moderate cases, severe COVID-19 cases had lower hemoglobin [weighted mean difference (WMD), − 4.08 g/L (95% CI − 5.12; − 3.05)] and red blood cell count [WMD, − 0.16 × 1012 /L (95% CI − 0.31; − 0.014)], and higher ferritin [WMD, − 473.25 ng/mL (95% CI 382.52; 563.98)] and red cell distribution width [WMD, 1.82% (95% CI 0.10; 3.55)]. A significant difference in mean ferritin levels of 606.37 ng/mL (95% CI 461.86; 750.88) was found between survivors and non-survivors, but not in hemoglobin levels. Future studies should explore the impact of iron metabolism and anemia in the pathophysiology, prognosis, and treatment of COVID-19.

CHANGES IN THE MORPHOLOGIC STATE OF RED BLOOD CELLS IN PATIENTS WITH POLYTRAUMA

The measurement of RBC indices is of particular importance as an indirect intermediate component of the recovery process after exposure to hypoxia, ischemia, hypothermia in patients with traumatic disease due to polytrauma. The aim of this study was to evaluate the parameters of the morphologic state of red blood cells as a reaction of patients with traumatic disease. Materials and methods.A prospective study was carried out evaluation of 120 patients suffering from polytrauma. The patients were divided into 3 groups according to the principle of using additional substances as a part of IC. The parameters of morphologic state of red blood cells were studied. Results and discussion. The course of acute and early periods of traumatic disease has accompanied variability morphological forms of red blood cells, as evidenced by a decrease in MCV and RDW increased levels of the 3rd to 5th day. The optimized therapy used in the treatment of patients, has a positive effect on the parameters of red blood cells, contributes to a better recovery of red blood cells after hypoxia resulting multiple trauma. Conclusion. The study has demonstrated variations in the morphologic properties of red blood cells in the acute and early period of traumatic disease, as confirmed by pathological changes of the erythrocyte indices. The administration of the proposed therapy with the use of D-fructose-1.6-diphosphate sodium salt of hydrate has a positive effect on the parameters of red blood cells, contributes to a better recovery after hypoxia resulting multiple trauma. Given this, the calculations of the red blood cell indices may carry additional diagnostic information, which allows identify trends for not obvious pathological changes and quality of treatment.

A novel SPTB gene mutation in neonatal hereditary spherocytosis: A case report.

The aim of the present study was to enhance the understanding of the diagnosis and treatment of neonatal hereditary spherocytosis (HS). Gene sequencing and analysis was performed for the crucial splicing signals on the exons and introns of the 302 known pathogenic genes [including ANK1, SPTAN1, SPTA1, EPB42, SLC4A1, and SPTB] that are associated with this genetic deficiency of erythrocytes. A 26-day-old female presented with jaundice, anemia, an increased count in peripheral blood reticulocyte and spherocytes and a positive acidified glycerol hemolysis test. Gene sequencing revealed a novel mutation of c.3737delA (p.Lys1246fs) in the exon 16 of SPTB (14q23|NM_000347.5) gene in the patient and her father. The mutation was a frame-shifting mutation, which may result in the truncation of β-haemoglobin in the erythrocyte membrane can lead to loss of normal function, leading to the occurrence of diseases, including jaundice and hemolytic anemia. For neonates with jaundice and anemia, family history, erythrocyte index and peripheral blood smear findings have been indicated to contribute to the diagnosis of HS. In the current study, gene sequencing was indicated to be helpful for the diagnosis of HS. A novel mutation of SPTB gene was identified, which may be pathogenic via modulating the activity of β-spectrin in the erythrocyte membrane.

Mutation spectrum and erythrocyte indices characterisation of α-thalassaemia and β-thalassaemia in Sichuan women in China: a thalassaemia screening survey of 42 155 women

AimsThe present study aims to investigate the mutation spectrum of thalassaemia, and characterise the erythrocyte indices of thalassaemia in a large cohort in Sichuan, which is a province with a...

The Influence of Floorball on Hematological Parameters: Consequences in Health Assessment and Antidoping Testing.

Assessment of hematological parameters is common in sports medicine. Although physical exercise is an important preanalytical variable, data about acute hematological changes after high-intensity intermittent exercise are scarce. This study aimed to examine floorball as a potential preanalytical variable for hematological parameters used in health assessment and antidoping testing. Twenty-three professional male floorball players participated in a floorball game. Hematological parameters including hemoglobin, erythrocyte count and erythrocyte indices, reticulocytes, white blood cells (WBC), platelets, reticulocytes, and OFF-hr score were assessed at baseline, immediately postgame, and at 2 h postgame. Median hemoglobin concentration decreased significantly from 146 g/L pregame to 141 g/L immediately postgame (p < 0.001). WBC count increased from 7.2 × 109/L pregame to 10.1 × 109/L 2 h postgame (p < 0.001). The median OFF-hr score decreased from 99.5 to 94.2 immediately postgame and remained significantly lower than baseline at 2 h postgame (94.4, p=0.030). Looking at individual results, the highest OFF-hr score increased from 120 at baseline to 124 at 2 h postgame. Our findings suggest that participation in a floorball game affects several hematological parameters and consequently can affect health assessment and antidoping testing.

Erythrocyte Morphology in Women of Reproductive Age (WORA) with Anemia

Anemia is a decrease in the number of erythrocytes in the blood circulation or the level of hemoglobin that is less than normal. The three major body mechanisms that cause anemia are excessive destruction of erythrocytes, blood loss, and decreased erythrocyte production. Based on the Basic Health Research (Riskesdas) in 2013, the prevalence of anemic women of reproductive age (WORA) aged 15-44 years in Indonesia was 35.3%. Anemia is classified based on the morphology of erythrocytes, including hypochromic microcytic, normocytic normochromic, and macrocytic. Erythrocyte morphology can be observed using peripheral blood smear examination. The objective of this study was to determine the morphology of erythrocytes in anemic women of reproductive age. This study belongs to descriptive research. The population of the study was 136 women of reproductive age, covering the students of D-IV in Medical Laboratory Technology at Setia Budi University. Forty-one respondents suffering from anemia were taken using a purposive sampling technique. The types of anemia were determined with examination using an Easy Touch hemoglobinometer with the Hb level of less than 12g/dL. Preparation of peripheral blood smear examination using EDTA venous blood and stained with Giemsa. Microscopic examination was performed with 1000x objective magnification. The peripheral blood smear reading showed the erythrocyte morphology that includes normocytic normochromic (38 samples or 93%), microcytic hypochromic (three samples or 7%), and poikilocytosis consisting of teardrop cells, target cells, ellipstocytes, and stomatocytes (five samples or 18%). Further study is required to investigate the correlation of erythrocyte index and peripheral blood smear in anemia.

Modulatory effects of melatonin on erythrocyte indices in Arabian stallions following a 30-km sub-maximal exercise

Experiments were performed to determine the effects of melatonin on erythrocytes indices in Arabian stallions following a sub-maximal exercise of 30 km. Twelve, apparently, healthy stallions of average age 6.3 ± 4.4 years and mean weight 403.44 ± 12.46 kg were used. They were divided into two groups of six stallions each. The stallions in MT which was the experimental group were administered with melatonin before exercise while stallions in the second group (NT) which served as the control were not administered with melatonin. Erythrocyte count in the MT group was higher (p = 0.023) than the count obtained in the NT group. Packed cell volume of NT group was lower (p = 0.038) than the value obtained in the MT group post-exercise. Haemoglobin concentration of the MT group was higher (p = 0.041) than the value obtained in the NT group after exercise. The values of mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration of the MT group were higher than the corresponding values recorded in the NT group (p = 0.033, 0.029 and 0.025 respectively). It was concluded that melatonin enhanced erythrocytes indices in the stallions studied.

Significant association between the preoperative erythrocyte mean corpuscular volume and infectious complications after pancreaticoduodenectomy.

An organ/space surgical site infection reportedly develops in 20% of patients who undergo pancreaticoduodenectomy (PD). The present study aimed to identify the predictors for developing severe infectious complications after PD. We retrospectively reviewed the records of 115 consecutive patients who underwent PD at Ehime University Hospital between January 2013 and January 2020. Severe infectious complications were defined as Clavien-Dindo classification grade ≥ III postoperative complications related to bacterial or fungal infections, including clinically relevant postoperative pancreatic fistula (CR-POPF). The patient characteristics, blood chemistry data, body composition data and operative data were evaluated as potential predictors of severe infectious complications. We also evaluated the erythrocyte indices, such as the mean corpuscular hemoglobin, the mean corpuscular hemoglobin concentration, and the mean corpuscular volume (MCV). Among 115 patients, 25 (21.7%) developed severe infectious complications, which included 20 (17.4%) cases of CR-POPF. According to multivariate analyses, MCV > 97.4fL, C-reactive protein (CRP) > 1.2mg/dL and diameter of main pancreatic duct < 5mm were independent predictors of severe infectious complications (odds ratio, 13.891, 7.356 and 4.676, respectively, 95% confidence interval, 3.457-55.815, 1.868-28.964 and 1.391-15.716, respectively). Preoperative high MCV/CRP values and a small main pancreatic duct are predictive factors associated with severe infectious complications after PD.

Erythrocyte Characteristics and the Risk of Depression in Late Life: A Population-Based Prospective Study

ObjectivesInflammation and vascular dysregulation may contribute to the development of depression and impose a burden on erythropoiesis. This study aimed to identify the association of erythrocyte indices with the severity of depressive symptoms and risk of developing depressive disorders in the older people. DesignA prospective cohort study on a randomly sampled Korean older population; the baseline assessment from 2010 to 2012, the first follow-up assessment from 2012 to 2014, and the second follow-up assessment from 2014 to 2016 (mean follow-up duration = 3.4 years). SettingA nationwide and community-based cohort. ParticipantsA total of 4451 Koreans aged 60 years or older. MethodsWe examined the associations of the values and changes in mean corpuscular volume, mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) with the risk of prevalent and incident depression using logistic regression analyses. ResultsHigh MCH and MCHC in female participants and high MCHC in male participants were associated with high geriatric depression scale scores and risk of prevalent depression. In female participants, high- and middle-MCH tertile groups showed a 2.68- and 2.34-fold higher risk of incident depression than did the low tertile group. In male participants, the high-MCH tertile group showed a 1.79-fold higher risk of incident depression than did the low tertile group. In both sexes, the participants whose MCV changed to the high or middle tertile or remained in the high or middle tertile during the follow-up period, and whose MCH increased to the high tertile or remained in the high tertile, were at a higher risk of incident depression. Conclusions and ImplicationsChanges in erythrocyte may be associated with the risk of depression in older adults. This prospective study proposes a new perspective of the old hematologic parameters for understanding the pathophysiology of late-life depression.

Plasma BDNF is a more reliable biomarker than erythrocyte omega-3 index for the omega-3 fatty acid enrichment of brain

Enriching brain DHA is believed to be beneficial for the prevention and treatment of several neurological diseases, including Alzheimer’s disease. An impediment in assessing the effectiveness of the treatments is the lack of a reliable biomarker for brain DHA. The commonly used erythrocyte omega-3 index is not suitable for brain because of the involvement of unique transporter at the blood brain barrier (BBB). We recently showed that dietary lysophosphatidylcholine (LPC)-DHA significantly increases brain DHA, which results in increase of brain BDNF. Since there is bidirectional transport of BDNF through the BBB, we tested the hypothesis that plasma BDNF may be used as biomarker for brain DHA enrichment. We altered the brain DHA in rats and mice over a wide range using different dietary carriers of DHA, and the correlations between the increase in brain omega-3 index with the increases in plasma BDNF and the erythrocyte index were determined. Whereas the increase in brain omega-3 index positively correlated with the increase in plasma BDNF, it negatively correlated with the erythrocyte index. These results show that the plasma BDNF is more reliable than the erythrocyte index as biomarker for assessing the effectiveness of omega-3 supplements in improving brain function.

Effects of Fruits of Lapsi (Nepali Hog Plum) Choerospondias axillaris (roxb.) b.l.Burtt &amp; a.w.Hill on Some Blood Parameters of Major Carp rohu Labeo rohita(H) During Intensive Aquaculture

The complete blood cell count (CBC) is an important and powerful diagnostic tool to monitor the health status of fish in response to changes related to nutrition, water quality, and disease in response to therapy.Thus, the present study was about to know the effect of lapsi fruit pulp (Choerospondias axillaris) on some blood parameters in the fingerlings of rohu Labeo rohita cultured in Corona of Agriculture Hatchery farm, Chitwan, Nepal. Altogether eighteen hapas made of heavy-duty nylon net (1.5m3) were kept and placed inside the pond, distributed linearly, and then 270 fingerlings (@15 fishes/ hapa) were kept distributed randomly. Six practical diets like T1 (0.0 g kg-1), T2 (0.1 g kg-1), T3 (0.2 g kg-1), T4 (0.4 g kg-1), T5 (0.8 g kg-1) and T6 (1.6 g kg-1) were prepared. Feed containing 40% protein was supplemented with the ethanol extract of lapsi fruits. At the end of 90 days of the feeding trial, a significant difference (P&lt; 0.05) in blood parameters were observed between the treated and control diet-fed groups. Hemoglobin (Hb), white blood cells (WBCs), red blood cells (RBCs), packed cell volume (PCV), and other erythrocyte indices were recorded higher in the treated groups. RBC, WBC, Hct, and Hb were found significantly higher in the T4 (0.4 %) diet-fed group. The study showed a minimum of 0.4 % (0.4g kg-1) lapsi fruit extract needed in fish feeds to increase blood parameters to enhance growth and immunity.

Biochemical Manifestations of Gastroesophageal Reflux Disease Progression in Children: A Single Center Case-Control Study

Gastroesophageal reflux disease (GERD) is a common digestive condition, representing one of the most frequent reasons for medical examination, especially in pediatric gastroenterology departments. GERD could be associated with biochemical alterations representing either its systemic manifestations or markers of complications. The aim of our paper was to evaluate biochemical parameters secondary to GERD in children. Two hundred and sixty-seven children of both genders aged between 1 month and 18 years who displayed suggestive symptoms for this condition were included in the study and were monitored for a period of 5 years. Depending on the range of symptoms and technical possibilities, the following procedures/investigations were performed: esophageal pH monitoring and imagistic or endoscopic examination, besides specific biochemical investigations. The cohort was sub-divided into two groups: one that included 213 children with confirmed GERD who represented the study group and 54 healthy children where GERD had been excluded, the control group. Out of all the investigated children, 39.0% displayed low hemoglobin values, 43.7% displayed low values of erythrocyte indices (MCH), and 68.5% had increased erythrocyte sedimentation rate (ESR) values, while increased eosinophil levels were recorded in 46.9% of the cases. Such parameters were proven to be a biomarker of suspected eosinophilic esophagitis, whereas 32.9% of the cases displayed high blood glucose values that could be correlated with gastroesophageal reflux symptoms. Other measured parameters (such as magnesium, aminotransferases and proteins) remained within the normal limits, without statistically significant differences compared to in the control group. This condition is diagnosed based on invasive investigations, which are often difficult to accept by the patients’ parents. The biochemical modifications correlated to the clinical manifestations can anticipate the progression of the disease, thus limiting the necessity of performing invasive diagnosis tests.

Comparative evaluation of two anticoagulants used for the analysis of haematological, biochemical parameters and blood cell morphology of himalayan snow trout, Schizopyge plagiostomus

The aim of the present study is to evaluate the effect of lithium heparin and K2EDTA on haematology, biochemical parameters and blood cell morphology of snow trout Schizopyge plagiostomus inhabiting in Kashmir Himalayan region. The haematological parameters such as hemoglobin (Hb), red blood cellcount (RBC), white blood cell (WBC), haematocrit (Hct), erythrocyte indices; mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), produced certain significant differences in their values with respect to both anticoagulants. Higher values of Hct, MCH and MCV while lower RBC count and MCHC values were recorded with a sample treated with K2EDTA compared to sample treated with lithium heparin. However, no significant change in Hb and WBC count was observed in the values of samples treated with both the anticoagulants. In the present study, the effect of two anticoagulant in biochemical parameters revealed that the alkaline phosphatase (ALP) and aspartate transaminase (AST) produced significant (P < 0.05) differences. Higher AST and lower ALP activity were recorded in the sample treated with K2EDTA compared to overall values in serum. Morphometric analysis showed an increase in the RBC perimeter area in samples treated with K2EDTA as compared to samples treated with lithium heparin. On the basis of above findings, it is recommended that in fish blood analysis heparin as anticoagulant could be useful as compared to K2 EDTA.

Effects of subacute oral administration of aqueous extract of Macaranga barteri Müll.Arg (Euphorbiaceae) leafon anthropometric and haematological parameters in rats.

Macaranga barteri is a plant used in traditional medicine to treat many illnesses. Previous studies showed the efficacy of the aqueous extract of leaf ofthis plant (AEMb) in the treatment of gastric ulcer within the dose range of 125-500mg/kg body weight (b.w.). This study aims at evaluating the safety of AEMb on anthropometric and haematological parameters in wistar rats. Seventy rats were divided into seven groups of ten rats each, including five males and five females. The control group was repeatedly administered by gavage with distilled water at 1ml/100g for 28days while test groups 2, 3, 4 and 5, were repeatedly gavaged with AEMb at the doses of 125, 250, 500 and 1000mg/kg b.w. respectively. As for satellites (groups 6 and 7), they received daily and respectively distilled water at 1ml/100g b.w. and AEMb at the dose of 1000mg/kg b.w. The results showed that AEMbcaused no significant changes in the behaviour of rats and the weight of the organs removed (kidneys, liver, spleen, and heart) and their relative weights at the end of the 28days of treatment. However, the body weight and the amount of food consumed by animals treated with AEMb at the doses of 250, 500 and 1000mg/kg b.w. increased significantly (p < 0.05) from the third week compared to control group. Haematological analysis revealed a non-significant increase (p > 0.05) in leukocyte lineage and platelet level infemale rats at the studied doses. However, a significant (p < 0.05) increase in platelet level was recorded in male rats at 1000mg/kg b.w. A significant (p < 0.05) increase in erythrocyte and hemoglobin levels at the doses of 250, 500 and 1000mg/kg b.w. in treated animals was also revealed. In conclusion, repeated administration of AEMb over 28days to rats was safe on leucocyte lineage and most of erythrocyte indices at doses ranging from 125 to 1000mg/kg b.w. Nevertheless, the use of this extract caused a transient increase of erythrocyte, hemoglobin and platelet levels 2weeks after the end of AEMb administration, butthese effects disappeared. So, the subacute oral administration of AEMbrevealed few potential toxic effects overall.

P0861THE RELATIONSHIP BETWEEN HEPCIDIN-25 AND BONE MARROW IRON IN ANEMIC, NON-DIALYSIS, CHRONIC KIDNEY DISEASE PATIENTS

Abstract Background and Aims Hepcidin-25 (Hep25) is a key known regulator of iron metabolism and its interactions with inflammation, iron stores and erythropoietic activity were involved in the pathogenesis of chronic kidney disease (CKD)-associated anemia. Therefore, our aim was to assess the determinants of serum Hep25 level in non-dialysis CKD patients. Method In this cross-sectional, single-center study, 52 subjects (56% men, 65±13 years) with CKD [estimated glomerular filtration rate, eGFR 14.5 (95%CI 16 to 25) mL/min] and anemia [hemoglobin, Hb 9.8 (95%CI 9.2 to 9.9) g/dL], not treated with erythropoiesis-stimulating agents (ESA) or iron in the previous 6 months, were enrolled. Patients with anemia of other causes than CKD, active infectious and inflammatory diseases, malignancy, severe hyperparathyroidism, transfusions during the last 3 months, and immunosuppressive therapy were excluded. The iron status was evaluated using both peripheral and central parameters. The iron stores were assessed by serum ferritin (Fer) and iron content in bone marrow macrophages (iMf, measured quantitively on a scale from 0 to 6). The iron available for erythropoiesis was assessed by transferrin saturation (TSAT) and the percentage of sideroblasts (%Sb). Anemia was further evaluated by a peripheral blood smear, erythrocytes indices and reticulocyte index. Serum Hep25 and erythropoietin (Epo) were assessed by ELISA (Bachem®, and Abcam® 119522, respectively). C-reactive protein (CRP), albumin, and parameters of kidney disease (eGFR, proteinuria) were also measured. Mann-Whitney, Kruskal-Wallis, Chi2 tests, Spearman bivariate correlation and multiple linear regression were used for statistical analysis. Results The median serum Hep25 of the whole cohort was 82.1 (95%CI 68.7 to 92.1) ng/mL. According to median Hep25, subjects were clustered in Group 1 (below median - G1) and Group 2 (above median - G2). %Sb and reticulocyte index had higher levels in G2 than in G1 [9 (95%CI 5 to 14) vs. 5 (95%CI 4 to 7) %, p=0.003 and 0.55 (95%CI 0.39 to 0.77) vs. 0.41 (95%CI 0.32 to 0.58), p=0.05, respectively], while the proportions of subjects with iMf suggestive for iron deficiency or iron overload were similar in G2 and G1 (38% vs. 50%, p=0.40, and 26% vs. 23%, p= 0.75, respectively). Peripheral blood smear, peripheral iron indices and all the other studied parameters were also alike. In bivariate analysis, Hep25 was positively associated both with indices of iron stores, i.e. Fer (rs = 0.30, p=0.03) and iMf (rs = 0.34, p=0.01) and indices of iron available for erythropoiesis, i.e. %Sb (rs = 0.55, p&amp;lt;0.001) and (marginally) with TSAT (rs = 0.26, p=0.06). Meanwhile, Hep25 was not related to serum Epo, CKD parameters or inflammation markers. In a multivariate linear regression model that explained 28% of Hep25 variation, the percentage of bone marrow sideroblasts, i.e. the tissue iron available for erythropoiesis, was the only independent determinant of Hep25: Variables entered in the first step: reticulocyte index, percentage of medullary sideroblasts (%Sb), iron content in the bone marrow macrophages (iMf), serum ferritin, and transferrin saturation Conclusion In stable patients with advanced CKD, not treated with ESA or iron, with low to moderate inflammation, serum hepcidin was related only to bone marrow iron available for erythropoiesis, suggesting that in this clinical setting the need of iron for erythropoiesis prevails over inflammation in regulation of hepcidin synthesis.

Performance of complete blood count (CBC) upon use of different anticoagulants in rats

Complete blood count (CBC) is used as an index of health status of human and different animals as well as to diagnose a variety of diseases. Therefore, there is a growing need of using the most suitable anticoagulant to obtain the most appropriate hemogram. The present study was designed to assess the effect of different anticoagulants viz. Heparin, Sodium Citrate and EDTA on complete blood count (CBC) in rat with a view to choosing the best suitable candidate among the common anticoagulants. A total of 30 samples out of which 10 were for each type anticoagulant were collected from 10 apparently healthy rats of Long Evans strain. From each rat 6 ml of blood was drawn and subsequently divided into three different test tubes with three different anticoagulants. The samples were analyzed for their complete blood count (TEC, TLC, Hb, Hct, DLC, absolute leukocyte count, Red Cell Indices, RDW-SD, RDWCV, Platelet, MPV, PCT and PDW) using Sysmex XT-1800i Auto hematological analyzer. Results showed a significant (p&lt;0.05) decrease in TLC, absolute neutrophil, and platelet count, and a significant (p&lt;0.05) higher level of relative lymphocyte count in Sodium Citrate treated sample than with Heparin and EDTA. No significant changes were observed for RBC or erythrocyte indices among the three different groups. It can be concluded that samples treated with Sodium Citrate results in significant change in blood parameters. Therefore, it is better to use Heparin and EDTA than Sodium Citrate for complete blood count (CBC).&#x0D; Asian J. Med. Biol. Res. March 2020, 6(1): 16-21

Effect of probiotic Lactobacillus paracasei on hematology and relative weight of lymphoid organs of broiler

One alternative approach can be done to replace the antibiotic growth promotant function (AGP) in broiler, which is the use of probiotic microorganisms. The aim of this study was to look at the effects of probiotics on Lactobacillus paracasei on hematological conditions and lymphoid organs. This study used a completely randomized design consisting of 4 treatments with 5 replications, each replication using 4 broiler chickens. Treatment consists of controls (0 mL/day), probiotics 1 mL/day, probiotics 3 mL/day, and probiotics 5 mL/day. The probiotics used in this study, namely Lactobacillus paracasei (5.8 x 107 CFU/mL) were given through drinking water. The results showed that the treatment had no significant effect (p>0.05) on the number of erythrocytes, hematocrit percentage, hemoglobin level, number of leucocytes, and erythrocyte index. Likewise, treatment had no significant effect (p>0.05) on the relative weights of bursa fabricius, thymus, and spleen exchanges. The values shown in hematology parameters are all within the normal range. Whereas in the lymphoid organ parameters there is a tendency for the relative weight of lymphoid organs to be greater in the treatment of Lactobacillus paracasei than in controls. Probiotics Lactobacillus paracasei used in this study did not respond poorly to hematological features and can be used as an immunomodulator in broilers.

Influence of zinc nanoaquacitrate on the immuno-physiological reactivity and productivity of the organism of rabbits

Zinc is necessary for maintaining the immune status, and its deficiency in the organisms of animals is usually accompanied by the condition of immune deficiency. The objective of the study was determining the effect of different amounts of zinc on the immune-biological reactivity and productivity of the organism of rabbits after their weaning on the 50th and 86th days of life. For the study, rabbits with the weight of 1.2–1.4 kg were selected and divided into four groups (control and three experimental). The rabbits of the control group were fed with unlimited balanced granulated compound feed, and had free access to water. The animals of the I, II and III experimental groups were watered with zinc nanoaquacitrate in the amounts of 0.25, 0.50 and 0.75 mg of Zn/kg of body weight. Compared with the control group, watering of the animals of the experimental groups with zinc nanoaquacitrate to a greater extent affected the content of phagocytic activity, lysozymic and bactericidal activities of the blood serum as integral factors of non-specific cellular and humoral resistance of the organism, which manifested in the increase in their content in blood on the 12th, 24th and 36th days of the experiment. Use of organic supplement in the diet of rabbits had a stimula­ting effect on the functioning of the immune system of their organism, which was seen in the higher content of total immunoglobulins, sialic acids and ceruloplasmin in the blood of animals watered with zinc nanoaquacitrate in the quantities of 0.50 and 0.75 mg of Zn/kg of body weight on the 24th and 36th days of the experiment. Use of organic compound of zinc in the diet caused high parameters of growth of the organism of rabbits during the period of 36 days, which manifested in the highest parameters of average-day increments and body weight on the 86th day of the life of the rabbits from the III experimental group, which received zinc nanoaquacitrate in the amounts of 0.75 mg of Zn/kg of body weight compared with the control group. Watering rabbits with zinc nanoaquacitrate during the study was accompanied by probable changes in the number of erythrocytes, concentration of hemoglobin and erythrocyte indices, which could indicate a positive effect of the employed additives on the hematopoietic function of the rabbits’ organism. The data of the performed experiment suggest that watering with larger amounts of organic compound of zinc has a positive effect on the processes of formation of immuno-physiological reactivity of the rabbits’ organism and increase in their productivity. The practical purpose is the study of the impact of watering with zinc nanoaquacitrate on the immuno-biological reactivity of the organism of rabbit dams during the period of lactation.

Comparison of erythrocyte indices and haematological indices as markers of iron status of lagos children with cyanotic congenital heart disease

Background: There are very few reports on the erythrocyte indices of children with cyanotic congenital heart disease in Nigeria. The study aims to document and compare the erythrocyte indices of children with cyanotic congenital heart disease attending the cardiology clinic of the department of Paediatrics, Lagos State University Teaching Hospital, Ikeja, Lagos.