Erythrocyte Indices Research Articles

The positive effect of ω-3 polyunsaturated fatty acid supplementation on the cognitive function of children and adolescents with lipid metabolism disorders

Aim. To study the effect of ω-3 polyunsaturated fatty acids (PUFAs) on the cognitive functions of children with lipid metabolism disorders of different ages. Materials and methods. The study included 87 children aged 6 to 17 years who had not previously received ω-3 PUFA supplements. All subjects were tested twice, with an interval of 6 months, to determine the ω-3 erythrocyte index by gas chromatography-mass spectrometry and to study attention span and focusing using the correction task method (Bourdon test). The cohort was randomly divided into two groups: the main group, which included 60 children who received the ω-3 supplements, and the control group, which included 27 children who did not receive any nutritional supplements. Results. In children of the main group, after receiving the Norwegian fish oil NFO® at 1000 mg for 6 months, an increase in the erythrocyte ω-3 index was observed, while in the control group, it did not change significantly. A statistically significant correlation was found between the adequacy of ω-3 PUFA and the attention span and focusing. Conclusion. The results of the study showed the effectiveness of ω-3 PUFA supplementation at 1000 mg/day for 6 months and confirmed the positive effect of ω-3 PUFA on the cognitive sphere of children and adolescents. High-quality food supplements with ω-3 PUFAs at 1000 mg/day can be recommended to develop intellection, memory, and attention span and focus in children and adolescents.

Profile Laboratory of Thalassemia in the General Hospital Dr. Zainoel Abidin Banda Aceh

Thalassemia refers to the globin gene abnormality that affects the a and b globin genes. A comprehensive peripheral blood laboratory examination, Mentzer index, blood morphology, and Hb analysis were all performed on every patient with thalassemia who attended treatment as part of this analytical observational study. This research aims to know the profile of laboratory examinations of thalassemia patients in the Dr. Zainoel Abidin General Hospital (RSUZA) Banda Aceh. The study's target population was 547 individuals, with 278 males and 269 females making up the male and female split, respectively (50.7% and 49.3%). With a total of 197 subjects (36.4%), this study revealed that the subjects were most frequently found in the age range of 0–5 years. Only 8 subjects, or 1.5%, of the population in the over-65 age group, were detected. The typical value of the erythrocyte index further fell and was discovered in 469 participants with a value of 85.3%. A total of 504 patients, or 91.6%, had more elevated RDW features. Results for the Mentzer index with 432 subjects were determined to be 13 were 78.5%. In the 450-subject hypochromic microcytic group, subjects were most frequently observed (81.8%). Blood morphology features from 505 participants, or 91.8% of the total, showed no additional blood morphology characteristics in normoblasts, and from 361 subjects, or 65.6% of the total, showed no target cells. When a person has anemia or a family history of thalassemia, it is strongly advised that they get a full blood count (CBC). The proper laboratory testing is still necessary for the health issue of thalassemia.

Effect of Incorporating Soursop (Annona muricata) Leaves Powder on Reproductive Performance of Japanese Quail (Coturnix coturnix japonica)

Introduction: Soursop leaves are rich in various molecules, including total phenols, terpenes, and steroids, which possess a range of pharmacological properties that can be utilized in animal production to enhance both growth and reproduction of animals. The present study aimed to evaluate the effect of incorporating soursop (Annona muricata; A. muricata) leaves powder into feed on the reproductive performance of Japanese quail. Materials and methods: A total of 80 Japanese quails (64 females and 16 males) aged two weeks were randomly divided into four experimental groups, labeled T0, T1, T2, and T3, and received feed additives with 0 mg/kg body weight (bw), 250 mg/kg bw, 500 mg/kg bw, and 750 mg/kg bw of powdered soursop leaves, respectively. Additionally, water was provided ad libitum, and the quails' weights were measured every 7 days for a period of 75 days. At the end of the period, 12 female quails from each group were sacrificed after 24 hours fasting period. Blood was collected for hematological (Leukocyte, Erythrocyte, and Platelet indices) and serum biochemical (total serum cholesterol, total proteins, albumin, and globulin, Aspartate aminotransferase, Alanine aminotransferase, Urea, and Creatinine) analysis. The males were also sacrificed to evaluate the spermatozoa characteristics (mobility, concentration, and viability). Results: No statistical significant changes in growth characteristics or hematological parameters were observed. However, biochemical parameters increased significantly with the inclusion of Soursop (A. muricate) in quail feed, including increased total cholesterol, total protein, and globulin levels, and decreased malondialdehyde levels. This effect was most significant at dosage of 500 mg/kg bw. Serum levels of urea, Alanine aminotransferase, and Aspartate aminotransferase were not significantly affected by A. muricata whatever the concentration considered. A significant increase in fast progressive spermatozoa, along with a decrease in immotile spermatozoa, was observed with A. muricate at dosage of 500 mg/kg bw compared to the control. Sperm viability also increased significantly, particularly in live at dosage of 500 mg/kg bw. Significant increase was observed in fertility parameters, including increased fertility rate, hatchability rate of fertile eggs, total hatchability rate, and chick weight, along with decreased embryonic mortality at 500 mg/kg bw treatment compared to the control. Conclusion: In conclusion, the findings indicated that incorporating A. muricata leaf powder at 500 mg/kg bw into quail feed positively influences reproductive cells and boosts fertility growth promoters.

Anemia on Chronic Disease in Leprosy Before and After Completing Multi Drug Therapy

Leprosy infection can cause anemia due to chronic disease (ACD). Multidrug therapy (MDT) for leprosy patients can overcome the infection. This study aims to determine the comparison in the incidence of ACD before and three months after MDT to leprosy patients at Prof. Dr. I G.N.G. Ngoerah Central General Hospital. A cross-sectional analytical observational study was conducted using secondary data from medical records of leprosy patients. A total of 142 samples were taken through total sampling. Data taken included gender, domicile, type of leprosy suffered, incidence of ACD, hemoglobin levels, and erythrocyte index before and three months after completing MDT leprosy therapy. Data analysis was carried out with SPSS version 21 using chi-square and paired t tests (dependent t-test), p value <0.05 means significant. The incidence of ACD was significantly higher before leprosy MDT therapy compared to 3 months after completing leprosy MDT therapy (49.3% vs. 16.2%; p value <0.001) with a prevalence ratio of 3.043 (2.021-4.584) CI95%. The mean hemoglobin levels, MCV, MCH, and MCHC levels 3 months after MDT leprosy therapy were significantly higher than before receiving MDT therapy (13.73 ± 1.47 vs. 12.40 ± 2.06 g/dL; p value <0.001), (88.18±7.22 vs. 84.07±9.55 fl; p value<0.001), (28.32±2.76 vs. 26.72±3.17 pg/cell; p value <0.001), (32.03±1.30 vs. 31.69±1.47%; p value<0.001). The incidence of ACD was significantly higher before MDT compared to 3 months after MDT. Mean hemoglobin, MCV, MCH, and MCHC levels were found to be significantly higher 3 months after MDT compared to before receiving MDT.

Investigation of Hepatozoon Canis Related Alterations in the Haemato Biochemical Profile of Dogs

Aims: Hepatozoonosis, caused by a protozoan parasite Hepatozoon canis, is important for canine health due to its ability to cause chronic, debilitating disease, and the challenges it presents in terms of diagnosis and management. The present study was conducted to see the effect of H. canis infection on various haematological and biochemical parameters as compared to the normal healthy dogs in order to understand the disease pattern and suspecting the disease. Study Design: The clinically affected dogs that come to the veterinary hospital with symptoms of hepatozoonosis were included in the study. Haematological and biochemical parameters of dogs found positive for hepatozoonosis were analysed. The values of these parameters were subsequently compared to healthy animals in order to identify any significant difference caused by H. canis. Place and Duration of Study: The studied was conducted at Department of Veterinary Medicine, DUVASU Mathura, India. Total 212 blood samples were collected from the dogs that were brought for the treatment in between February 2023 to March 2024. Methodology: Total 212 dogs were included in the present study out of this 46 dogs were found positive with H. canis using PCR based molecular test. These positive animals were subjected to study the changes in biochemical parameters like blood urea nitrogen, creatinine, alkaline phosphatase, alanine transaminase, aspartate amino transferase, total leucocyte count, total erythrocyte count, haemoglobin, haematocrit, erythrocyte indices (MCV, MCH, MCHC), differential leucocyte count, platelet count and platelet indices (MPV, PDW) using autoanalyzer. Student's t-test in Graphpad Prism was used to compare these values to healthy animals. Results: The study confirms that Hepatozoonosis leads to significant haematological and biochemical changes in dogs. Infected dogs have lower Hb, TEC, and PCV than healthy animals, indicating anaemia. Thrombocytopenia and leukocytosis were also observed in the infected animals. Non-significant differences in MCV, MCH, and MCHC indicate normocytic, normochromic anaemia. Anisocytosis and non-regenerative anaemia is indicated by significant RDW-CV increases with decrease in MCV values. Increased liver-specific enzymes like ALT, AST, and ALP and kidney-related enzymes like BUN and CRT suggest hepatic and renal involvement in disease pathophysiology. Conclusion: In hepatozoonosis, haemato- biochemical parameters like non-regenerative anaemia. anisocytosis, thrombocytopenia, leukocytosis, low serum protein concentration and increased ALT, AST, ALP and BUN values may be used as non-specific biomarkers. The results can be used in making clinical decisions and monitoring therapeutic responses in cases of hepatozoonosis.

Influence of an iron dextran injection in various diseases on hematological blood parameters, including serum ferritin, neonatal dairy calves

BackgroundFeeding milk substitutes with low iron content or whole milk without iron supplementation is considered a major factor in developing iron-deficiency anemia in neonatal dairy calves. Young calves are often supplemented with iron dextran injections on the first day of life to prevent anemia. However, the effects of preventive treatment and the presence of disease on serum iron (Fe) concentrations, serum ferritin levels, and hematological blood parameters during the early neonatal stages have not been examined in detail. Therefore, we examined and evaluated the effects of iron dextran injections and health status on the development of hematocrit (Ht), red blood cells (RBC), hemoglobin concentration (Hb), erythrocyte indices (mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration), Fe, and serum ferritin concentrations in dairy calves within the first 10 days of life. The suitability of serum ferritin as a reliable indicator of anemia in very young calves was evaluated by correlating ferritin concentrations with known laboratory diagnostic parameters of anemia.ResultsIron supplementation significantly increased Fe levels (P = 0.048) but did not affect serum ferritin levels in neonatal calves. Fe concentrations were significantly lower in diseased than healthy calves (P = 0.0417). Iron supplementation significantly affected the health status, as observed in Ht (Ptreat=0.0057; Phealth=0.0097), RBC (Ptreat=0.0342; Phealth=0.0243), and Hb (Ptreat=0.0170; Phealth=0.0168). Serum ferritin levels did not significantly correlate with Fe levels. Both groups showed marked differences in ferritin levels, with the highest levels measured on day 2. Fe concentrations showed weak negative correlations with Hb and Ht levels on day 3 (ρ=-0.45; P = 0.0034 and ρ=-0.045; P = 0.0032, respectively). RBC count showed strong positive correlations with Hb and Ht levels (ρ = 0.91 and ρ = 0.93; P < 0.001).ConclusionIron dextran injections increased Fe concentrations but reduced Ht level, RBC count, and Hb level. The presence of diseases led to a reduction in Fe and higher values of Ht, RBC, and Hb in moderate disease than in severe disease. Due to physiological fluctuations during the first 3 days of life, serum ferritin level seems unuseful for evaluating iron storage before day 4 of life.

Indicators of iron metabolism in regular donors of blood platelets and plasma

Introduction. The high frequency of medical withdrawals from donatio ns due to a decrease in hemoglobin levels and the negative impact on the motivation and return to donation of temporarily withdrawn individuals dictates the need to study the effect of exfusion of blood and its components on iron metabolism.Aim: to evaluate the iron metabolism indicators in regular donors of platelets and plasma.Materials and methods. 99 platelet donors and 90 blood plasma donors who regularly participated in donations were examined. The comparison group consisted of 158 healthy individuals who had not previously donated blood and its components. Hemoglobin concentration, hematocrit, erythrocyte count and erythrocyte indices were assessed. The concentration levels of ferritin, iron, transferrin in blood serum as well as the values of total and unsaturated iron-binding capacity of se rum were studied, and the saturation coefficient of transferrin was calculated.Results. In men who donated platelets, a statistically significant decrease in the red blood cells count (p = 0.001), hematocrit (p = 0.014) and ferritin concentration (p &lt; 0.001) were found relative to those in the comparison group: 4.8×1012/l, 42.0 %, 28.9 ng/ml versus 5.0×1012/l, 44.0 %, 74.2 ng/ml respectively. In regular donors a significant increase in the medium of hemoglobin of red blood cells was noted: 30.8 pg (p = 0.008) and 31.4 pg (p &lt; 0.001) in men and 30.2 pg (p = 0.030) and 31.0 pg (p &lt; 0.001) in women with platelet and plasma exfusions respectively compared to 29.4 pg in men and 29.6 pg in women who participated in donation for the first time. In plasma donors of both sexes, the values of hemoglobin and its average concentration in the erythrocyte were significantly higher than in first-time donors: 154.0 g/l (p = 0.008), 35.7 g/dl (p &lt; 0.001) and 138.0 g/l (p &lt; 0.001), 35.2 g/dl (p &lt; 0.001) versus 146.0 g/l, 33.6 g/dl and 129.0 g/l, 33.8 g/dl in men and women, respectively.Conclusion. To prevent the development of iron deficiency in donors of blood components it is necessary to monitor the con-centration of serum ferritin and correct its reduced values by changing the donation regimen or prescribing iron-containing drugs. When selecting donors, attention should be paid to the frequency of apheresis, especially in men who regularly donate platelets.

The erythrocyte sedimentation rate and other markers of inflammation in cats tested for Leishmania infantum and feline immunodeficiency virus antibodies

BackgroundIn endemic areas, Leishmania infantum and feline immunodeficiency virus (FIV) co-infection occurs in cats, and may favour a progressive course of feline leishmaniosis. Abnormalities in serum protein fractions have been reported, but inflammation markers have scarcely been studied. Erythrocyte sediment rate (ESR) is a marker of inflammation that is poorly used in veterinary medicine, but it has been evaluated in EDTA blood using a recently introduced automatic device. We studied ESR and a pool of feline markers of inflammation (MoI) in cats L. infantum (Li+) and/or FIV antibody-positive (Li+FIV+/FIV+) with the aims (a) to evaluate ESR as MoI in cats with the infectious and clinical conditions considered and (b) to provide data about a pool of MoI never investigated in the feline infections studied and in other cat diseases before.MethodsThis prospective controlled study included 35 study group cats (Li+, n = 20; FIV +, n = 8; Li+FIV+, n = 7) and ten healthy antibody-negative control cats. Clinical findings at physical examination and selected clinical pathological abnormalities related to inflammation were statistically analysed in relation to the infectious status and ESR values.ResultsESR values were higher in Li+, FIV+, and Li+FIV+ cats compared with control cats, and 40% of the study group cats had ESR values above the reference interval (RI). ESR positively correlated with some positive MoI and negatively with some negative MoI studied. Additionally, a higher prevalence of ESR values above the RI has been detected in cats with hypoalbuminemia or hypergammaglobulinemia and higher ESR values were measured in cats with serum protein electrophoresis (SPE) fraction abnormalities. Correlations were also found with erythrocytes, hemoglobin, hematocrit and some erythrocyte indices. FIV+ and Li+FIV+ cats had a higher prevalence of increased ESR values, and almost all had SPE abnormalities and more severe clinical presentations compared with Li+ cats.ConclusionsAbnormal levels of MoI were found in almost all parameters studied, particularly in FIV+ and Li+FIV+ cats. Also, ESR can be used as a marker of inflammation in cats with L. infantum and/or FIV infection.Graphical

The Correlation of β-Adrenoreactivity of Erythrocyte Membranes with Clinical and Laboratory Parameters in Patients with Resistant Hypertension in the Presence and Absence of Type 2 Diabetes Mellitus

The peculiarity of the conjugation of β-adrenoreactivity of membranes (β-ARM) erythrocyte, as an indicator of the state of autonomic regulation of the cardiovascular system, with other clinical and laboratory indicators in drug-resistant arterial hypertension (RAH) with the absence and presence of type 2 diabetes mellitus (DM2) was investigated. It was shown that patients with RAH, regardless of the presence of DM2, were characterized by reduced adrenoreactivity, while the beta-ARM index of erythrocytes was more than 2 times higher than the reference values. The intergroup difference in the mean values of beta-ARM erythrocytes and the frequency of cases of exceeding the established reference values by this indicator did not have significant differences. In RAH, regardless of the presence of DM2, the beta-ARM erythrocytes index of erythrocytes is statistically significantly associated with the variability of blood pressure, the volume of daily diuresis and increased contractile capacity of the left ventricle. In addition, in the presence of DM2, the association of β-ARM erythrocyte with an increase in left ventricular elastance, heart rate variability, duration of arterial hypertension and DM2 was revealed. Thus, with the development of RAH in humans, the β-ARM erythrocyte indicator can be a new biomarker for personalized assessment of the activity of autonomic regulation of the cardiovascular system. CD2 expands the conjugacy of β-ARM erythrocyte with clinical and laboratory parameters.

GAMBARAN TIPE ANEMIA PADA IBU HAMIL BERDASARKAN NILAI INDEKS ERITROSIT DI RSIA RESTU IBU TAHUN 2022

Anemia in pregnant women is a global problem that impacts both mother and fetus. Anemia in pregnancy is associated with maternal morbidity and is an indirect cause of maternal death in the entire population of pregnant women. Classification of anemia based on the erythrocyte index value is divided into normochromic normocytic, normochromic macrocytic, hypochromic microcytic and hypochromic macrocytic anemia. The erythrocyte index values ​​are divided into Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC). The aim of the research is to describe the types of anemia in pregnant women based on the erythrocyte index value at RSIA Restu Ibu Sragen in 2022. This research uses a quantitative descriptive research type with a retrospective approach. The research sample was 51 medical records of pregnant women who experienced anemia at RSIA Restu Ibu Sragen in January-December 2022 using purposive sampling. The materials and tools used were secondary data from laboratory examination results of pregnant women diagnosed with anemia which included hematocrit, hemoglobin, index erythrocytes (MCV, MCH, and MCHC), Microsoft Excel and SPSS Version 21 software. Data analysis used analysis univariate. The results of the study concluded that pregnant women who experienced anemia at RSIA Restu Ibu Sragen were aged 21-34 years with a third trimester pregnancy and were primigravida. Based on the erythrocyte index, normocytic anemia was normochromic in 18 people (35.3%), microcytic hypochromic anemia in 20 people (39.2%), and macrocytic hypochromic anemia in 13 people (25.5%). So it can be said that most pregnant women experience microcytic hypochromic anemia.

Evaluation of Hematotoxicity in Female Wistar Rats Following Sub-Acute Inhalation Exposure to Polyethylene Microplastic

Polyethylene (PE) becomes a source of microplastics that can be widely distributed through the digestive and respiratory systems. However, its effects on blood cells are still being investigated. This study aims to analyze the impact of Polyethylene Microplastic (PE-MPs) exposure on the blood of female rats, including erythrocytes, leukocytes, and platelets. This study used female Wistar rats, which were divided into control and PE-MP groups. PE-MP was administered via whole-body inhalation at a concentration of 15 mg/m³ for 4 hours daily for 28 days. The absorption of plastic particles detected in the human bloodstream is likely to occur through mucosal contact (either through ingestion or inhalation). After the exposure period, the rats were euthanized to collect blood samples through the heart. A complete blood count was performed using an automatic hematology analyzer, and blood morphology was analyzed using thin blood smears. This study used the Mann-Whitney test. PE-MP exposure increased erythrocyte and platelet counts without a corresponding rise in leukocytes. Erythrocytes showed abnormal morphology (12.73% with ovalocytes and tear-shaped cells). Erythrocyte indices (MCV, MCH, MCHC) showed no significant differences. Platelet count rose by 1.7% (p-value= 0.017). Leukocyte and neutrophil counts were lower (0.84 and 0.94 times lower, respectively), while lymphocytes and monocytes were higher (1.03 and 1.61 times higher, respectively) in the PE-MP group compared to controls. The neutrophil-to-lymphocyte ratio did not differ significantly. PE-MP exposure in rats disrupts blood parameters, altering erythrocyte morphology and increasing platelet counts. Potential causes include oxidative stress, immune responses, and compensatory mechanisms. Study limitations include a small sample size and exclusive focus on inhalation exposure. Integrating multiple exposure routes (inhalation, ingestion, dermal) could offer a broader view of microplastic impacts. Future research with larger samples, diverse doses and durations, and exploration of additional markers or organ-specific effects is crucial for understanding PE-MP toxicity in real-world scenarios.

Effects of dietary Silybum marianum powder on growth performance, egg and carcass characteristics, immune response, intestinal microbial population, haemato-biochemical parameters and sensory meat quality of laying quails

The study aimed to assess the effects of different dietary Silybum marianum (SM, milk thistle) powder levels on growth performance, productivity, immunity, small intestine, haemato-biochemical parameters, meat quality, and egg and carcass characteristics of laying quails. The experimental subjects consisted of one hundred and eight 43-day-old quails divided into 3 treatments (0, 0.75, and 1.50% SM) with 4 replicates each. The egg characteristics and growth performance of the quails were evaluated. Quails were euthanized for evaluation of carcasses, microbiota, and sensory characteristics of meat. Blood samples were analyzed for haematology and biochemical profile. SM at 0.75% and 1.50% significantly (P < 0.05) increased feed intake, enhanced egg characteristics (number, weight, width, length, volume, weight of egg yolk, and eggshell thickness), jejunum and ileum length, spleen weight, lactobacillus population, sensory characteristics of meat, red blood cell (RBC), hemoglobin, erythrocytic indices, concentration of albumin, globulin and thyroid stimulating hormone (TSH). SM at 0.75% and 1.50% decreased (P < 0.05) carcass weight (abdominal fat, heart, neck, and pancreas), feed conversion ratio (FCR) based on eggs produced, percentages of heterophils and lymphocytes, concentration of lactate dehydrogenase, population of coliforms clostridia, and Escherichia coli. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and creatine kinase were not significantly (P > 0.05) altered by 0.75% and 1.50% SM. SM at both levels (0.75% and 1.50%) may improve growth, egg characteristics, immune response, intestinal morphology and microbiota, meat quality and erythropoiesis, and also lead to decreased cholesterol in laying quails. Economics can be improved, too. The authors recommend adding 1.0% of SM to quail diet.

Welfare and stress assessment of tourism carriage horses under real working conditions in Sicily

Animal welfare has become an increasingly important concern regarding equids working as carriage animals. In the present study, the changes in the markers of stress and inflammatory responses as a result of the work performed by tourism carriage horses under real working conditions in Sicily (Italy) were investigated. Twenty-two Standardbreds performed a normal working day in the carriage tourism business during the months of May, June and July 2022, consisting of one day of work for each month. Blood samples were collected in the stables at rest before the tour route (Pre; 07.00 AM) and within 10 min after the end of the workday (Post; 05.00 PM). Haematological parameters, serum concentration of cortisol, total proteins together with the globulin fractions were investigated before and after the carriage work. Environmental temperature, relative humidity and temperature humidity index (THI) were also assessed. The direct erythrocyte indices increased after work compared to rest condition (P < 0.05). The values of cortisol, total proteins and globulins were not affected by carriage work (P > 0.05), while, higher cortisol, total proteins, α1- and α2-globulins values were observed in July compared to May and June (P < 0.05). These changes are probably due to the increase in THI values which showed mild stress in June and high stress in July. This study suggests that the tourism carriage horses herein investigated have adapted to their work activity, however, avoiding working horses during the hottest hours of the day in the summer months is advocated.

TOTAL ERYTHROCYTE COUNT, HEMOGLOBIN HEMATOCRIT VALUE, AND RED BLOOD CELL INDICES OF DOG WITH ATOPIC DERMATITIS POST THERAPY OF ECO ENZYME

Atopic dermatitis is a common skin disorder in dogs. This study was aimed to determine the effect of eco enzyme on dogs suffering from atopic dermatitis in terms of changes in total erythrocytes, hemoglobin levels, hematocrit values and erythrocyte indices. Two groups of dogs with atopic dermatitis were used in this study: concentration A (three dogs) were bathed with 10% concentration of eco enzyme at intervals of three days, and concentration B (two dogs) were bathed with 10% concentration of eco enzyme at intervals of three days, then continued a week later with eco enzyme concentration of 2% at intervals once a week. Both groups were given treatment for five weeks. Hematology data were analyzed through tiered pattern analysis of variance followed by Duncan’s multiple range test using SPSS which showed that the concentration of eco enzyme and the duration of observation (weeks) had no significant effect on total erythrocytes, hemoglobin, hematocrit, MCH and MCHC, although they influenced MCV. Also, there was a real interaction between the concentration of eco enzyme and the duration of observation at MCH. It can be concluded that therapy with eco enzyme has not been able to improve total erythrocytes, hemoglobin, hematocrit, MCH and MCHC, but has been able to improve MCV in dogs with atopic dermatitis.

The Effect of Giving Pork Oil and Egg Yolk as Components of a High Fat Diet on The Hematological and Cholesterol Profiles of Mice (Mus musculus)

Background: This study examined the role of a high-fat diet, especially one containing animal fat, in its influence on the hematology of the organism, with mice as research subjects. Hematology, as the study of blood components, is the focus for analyzing the impact of a high-fat diet, which includes animal fats such as lard and egg yolks. Although fats are essential for cell function and nutrient absorption, excessive intake can increase the risk of serious health problems. Purpose: To fill the gap in knowledge by evaluating the effect of lard and egg yolk on the hematological profile of mice. Methods: True Experimental with a Pretest-Posttest Control Group design. The sampling technique used is Simple Random Sampling. The parameters yolk). The sampling technique used is Simple Random Sampling. The parameters observed include blood hematological profiles (Erythrocyte Count, Hemoglobin, and Erythrocyte Index) and cholesterol levels. Results: Expected to provide in-depth insight into the impact of high-fat diets on blood health, provide important contributions to understanding the human health implications, and support the development of more effective nutritional guidelines for managing diet and preventing metabolism-related diseases. Conclusion: It can be concluded that giving pork oil with added egg yolk can increase erythrocyte levels, hemoglobin profile, erythrocyte index (MCV and MCH), and cholesterol levels.

Risk factors and integrated assessment of health disorders in teachers of rural comprehensive schools

Teaching is an occupation with high stress levels and extremely poor health indicators, which tend to decline as working records become longer. The aim of this study was to investigate risk factors able to cause health issues in teachers of rural comprehensive schools and to determine the significance of clinical and laboratory indices as early indicators. The work uses the results of hygienic, clinical and laboratory studies aimed at assessing health and working conditions of teachers employed at comprehensive schools in rural areas of the Republic of Bashkortostan. Sanitary and hygienic conditions in classrooms of rural comprehensive schools were assessed in conformity with the Guide R 2.2.2006-05. Medical check-ups resulted in 255 various somatic diseases diagnosed in 70 teachers, overall, 3.64 diseases per one examined teacher. Laboratory tests revealed elevated hemoglobin levels in 20.00 % of the examined female teachers and low ones in 8.57 %. Erythrocytosis was established in 64.29 % of the examined teachers. We also established some slight changes in hematocrit levels, which were elevated in 4.29 % and low in 8.57 % of the examined teachers. Accordingly, in case hemoglobin levels were low, erythrocyte indices MCV and МСН went down as well in 30.00–32.86 % of the female teachers. The number of workers with elevated glucose and cholesterol levels went up as their work records grew longer (the correlation coefficients were 0.94÷0.98). Examination of microflora found in the upper airway mucosa revealed clinically significant contamination, which was mostly represented by cocci bacteria, the most significant microorganisms being Staphylococcus epidermidis, Staphylococcus spp and β-hаemolytic streptococci. Our integrated study has shown that working conditions at workplaces of teachers in rural comprehensive schools do not conform to safety standards as per work hardness and work intensity (intellectual, emotional and sensory loads). The study results confirm high importance of diagnostic methods as indicators of health issues in teachers employed at comprehensive schools. Preventive measures aimed at protecting teachers’ health in rural areas should consider peculiarities of pathomorphosis that determines their health status.

Факторы риска и комплексная оценка нарушений здоровья педагогов общеобразовательных школ в сельской местности

Teaching is an occupation with high stress levels and extremely poor health indicators, which tend to decline as working records become longer. The aim of this study was to investigate risk factors able to cause health issues in teachers of rural comprehensive schools and to determine the significance of clinical and laboratory indices as early indicators. The work uses the results of hygienic, clinical and laboratory studies aimed at assessing health and working conditions of teachers employed at comprehensive schools in rural areas of the Republic of Bashkortostan. Sanitary and hygienic conditions in classrooms of rural comprehensive schools were assessed in conformity with the Guide R 2.2.2006-05. Medical check-ups resulted in 255 various somatic diseases diagnosed in 70 teachers, overall, 3.64 diseases per one examined teacher. Laboratory tests revealed elevated hemoglobin levels in 20.00 % of the examined female teachers and low ones in 8.57 %. Erythrocytosis was established in 64.29 % of the examined teachers. We also established some slight changes in hematocrit levels, which were elevated in 4.29 % and low in 8.57 % of the examined teachers. Accordingly, in case hemoglobin levels were low, erythrocyte indices MCV and МСН went down as well in 30.00–32.86 % of the female teachers. The number of workers with elevated glucose and cholesterol levels went up as their work records grew longer (the correlation coefficients were 0.94÷0.98). Examination of microflora found in the upper airway mucosa revealed clinically significant contamination, which was mostly represented by cocci bacteria, the most significant microorganisms being Staphylococcus epidermidis, Staphylococcus spp and β-hаemolytic streptococci. Our integrated study has shown that working conditions at workplaces of teachers in rural comprehensive schools do not conform to safety standards as per work hardness and work intensity (intellectual, emotional and sensory loads). The study results confirm high importance of diagnostic methods as indicators of health issues in teachers employed at comprehensive schools. Preventive measures aimed at protecting teachers’ health in rural areas should consider peculiarities of pathomorphosis that determines their health status.

Evaluation of erythrocyte and reticulocyte indices in a newborn with severe hemolytic disease

Currently, significant progress has been made in the prevention, diagnosis and treatment of hemolytic disease of the fetus and newborn. However, the development of anemia in a newborn child due to this disease remains an urgent problem for both neonatologists and pediatricians. In such children, a comprehensive assessment of the hemogram is of particular importance. The study of erythrocyte and reticulocyte parameters of a general blood test is necessary to determine prognostic criteria for the restoration of hematopoiesis and determine the status of iron deficiency. A clinical observation of a newborn child with the development of anemia due to hemolytic disease of the fetus and newborn is presented (clinical case). A dynamic analysis of reticulocyte parameters (absolute and relative numbers) and reticulocyte fractions was carried out. An increase in reticulocytes (absolute and relative numbers) was established, mainly due to the fraction of immature reticulocytes during the development of anemia in a newborn child. Such indicators as the hemoglobin content in reticulocytes, the average hemoglobin content in an erythrocyte, the average hemoglobin concentration in an erythrocyte in a general blood test in an infant during the neonatal period remained within the reference values. The data obtained made it possible to determine a personalized approach to the treatment of anemia and avoid blood transfusion in this child.

Possibility of using erythrocyte indexes to predict clinical outcome in patients with burn injury

Possibility of using erythrocyte indexes to predict clinical outcome in patients with burn injury

Possibility of using erythrocyte indexes to predict clinical outcome in patients with burn injury

Possibility of using erythrocyte indexes to predict clinical outcome in patients with burn injury