AbstractThe performance of an assimilation system is strongly dependent on the quality of the error statistics used. A number of error statistics estimation and tuning methods have previously been developed to better assess and determine these statistics. Many of these are a posteriori methods which make use of quantities calculated during the assimilation procedure, while other a priori methods do not require information from the assimilation. In this study, we develop a conceptual framework that relates these methods when applied to error variance determination, where each method is associated with the minimization of a particular cost function. The minimization of these cost functions describes a fitting procedure that fits parts of the prescribed modelled innovation covariance to its observed values. Each method must in some way separate the innovation covariance into its contributions from the background and the observations, which are then used in the fitting procedure. It is shown that the examined a posteriori methods use the analysis filter to make this separation and that the minimization of their associated cost functions is done implicitly within the tuning procedure. Analytical expressions for the expectation value and variance of estimates for error variance scaling parameters are determined for each method. The expressions for the expectation values of these estimates show that the accuracy of each method is dependent on its ability to separate the background from the observation contributions to the innovation covariance. This separability is quantified by use of the Frobenius inner product between the background‐ and observation‐error covariances, which additionally allows for geometric interpretations of the covariances to be made. Comparisons between variance parameter estimates from different methods are made for the case of a 1D periodic domain.