<h3>Purpose/Objective(s)</h3> Many quality assurance procedures in radiotherapy are tedious and time-consuming but can be automated to improve the efficient and minimum human-errors. In this study we focused on the patient specific quality assurance (PSQA) procedure. The purpose was to develop a robust and quick software interface for Human Interactive Device (HID) operations and significantly accelerate the workflow. <h3>Materials/Methods</h3> The clinical PSQA system of our clinics consists of s treatment planning system (TPS), an oncology information system (OIS) and a measurement and analysis software. The complete PSQA process includes three main parts: (1) QA verification plan creation, (2) treatment plan loading to treatment machine and (3) measurement, data analysis and report. A homemade software tool, AUTOFLOW was developed as a general interface and installed on multiple workstations to automate the operations of local HID devices. The workflow of HID operations in these components was modularized for maximum efficiency and reliability. The required patient information for each computer was stored in a QA task file created from patient database and synchronized by the AUTOFLOW interface in individual workstations. All human operations on HID devices and communication between multiple computers were automated by the AUTOFLOW interface sequentially or parallelly during the entire PSQA process. <h3>Results</h3> We tested the developed automation workflow on 20 clinical QA plans in our current clinical environments. Overall, around 175 (±12) human HID operations on various workstations were removed from the PSQA procedure. On average, 8(±0.8) minutes was needed to complete a PSQA case with the AUTOFLOW software tool, compared to 13(±3.1) minutes for complete manual operations. These results represent a 38% saving in time. No errors were found for all operations performed with this interface. <h3>Conclusion</h3> The AUTOFLOW software interface was successfully developed and used to perform PSQA in our current clinical environment. This interface has substantially reduced the time, and eliminated user stress and input errors for our PSQA procedure. It can potentially work with other TPS, QA, R&V systems of our department to improve the efficiency and reduce human errors for other QA procedures. For future works we plan to improve the system flexibility/stability and implement this application to other QA procedures