Equus Burchellii Research Articles

Gasterophilus intestinalis infestation in lion (Panthera leo) and plains zebra (Equus quagga) in the Serengeti ecosystem: Morphological and molecular profiling.

This study was conducted to clarify the host specificity and the geographical distribution of Gasterophilus species (Diptera, Oestridae) in the Serengeti ecosystem. A total of 317 larvae were recovered from two common zebras (Equus quagga, formerly Equus burchellii) in Maswa Game Reserve, and 58 larvae were recovered from an African lion (Panthera leo) in the Serengeti National Park. The study emphasizes the rare occurrence of Gasterophilus sp. in lions, shedding light on the broader life cycle and physiological implications for hosts. Genetic analysis of cox2 genes from Gasterophilus species, sourced from a single geographic location, reveals significant genetic distinctions and host specificity. This study reports the first case of G. intestinalis infestation in an African lion in the Serengeti ecosystem, extending its known range from zebras and other equids, and highlighting ecological and veterinary implications. This unusual prey-predator transmission highlights the value of molecular taxonomic tools in clarifying host-parasite dynamics and guiding targeted conservation strategies.

The Population Size and Distribution of Diurnal Large Wild Mammals in the Southern Great Rift Valley, Ethiopia

The study was carried out to assess the population size and distribution of diurnal large wild mammals in the southern Great Rift Valley, Ethiopia. The study area was stratified into four habitat types: riverine forest, ground-water forest, grassland, and bushland. Samples of animals were surveyed through the transect method. The total number of individuals belonging to the 15 species observed was 1681 and 1245 during the wet and dry seasons, respectively. Burchell's zebra (Equus burchellii), Anubis baboon (Papio anubis), Vervet monkey (Chlorocebus pygerythrus), and Grant's gazelle (Nanger granti) were the most abundant species, while Abyssinian hare (Lepus habessinicus) and Bush duiker (Sylvicapra grimmia) were the least abundant species. The highest number of species has been supported by the bushland habitat, followed by open grassland, riverine forest, and ground-water forest in both seasons. Despite the park being home to various types of mammalian species, there is a need for conservation actions by the park management and other concerned bodies for the survival of those species in the area.

In Silico Infection Analysis (iSFA) Identified Coronavirus Infection and Potential Transmission Risk in Mammals.

Coronaviruses are a great source of threat to public health which could infect various species and cause diverse diseases. However, the epidemic’s spreading among different species remains elusive. This study proposed an in silico infection analysis (iSFA) system that includes pathogen genome or transcript mining in transcriptome data of the potential host and performed a comprehensive analysis about the infection of 38 coronaviruses in wild animals, based on 2,257 transcriptome datasets from 89 mammals’ lung and intestine, and revealed multiple potential coronavirus infections including porcine epidemic diarrhea virus (PEDV) infection in Equus burchellii. Then, through our transmission network analysis, potential intermediate hosts of five coronaviruses were identified. Notably, iSFA results suggested that the expression of coronavirus receptor genes tended to be downregulated after infection by another virus. Finally, binding affinity and interactive interface analysis of S1 protein and ACE2 from different species demonstrated the potential inter-species transmission barrier and cross-species transmission of SARS-CoV-2. Meanwhile, the iSFA system developed in this study could be further applied to conduct the source tracing and host prediction of other pathogen-induced diseases, thus contributing to the epidemic prevention and control.

Mitochondrial DNA genetic variations among four horse populations in Egypt.

Horses are one of the early domesticated animals in the world that changed societies and civilizations on a continent-wide scale. Due to the rare information about the genetic characterization of different horse populations in Egypt, this study aimed to identify the genetic biodiversity and relationships between four horse populations reared in Egypt. Genomic DNA was extracted and mtDNA region was amplified using polymerase chain reaction (PCR). The alignment of 384-bp amplified fragments showed the presence of 41 polymorphic sites resulting in 29 haplotypes which their sequences were submitted to GenBank under the accession numbers: KX909898-KX909926.The phylogeny tree for tested horses declared the presence of mixing maternal lineages between the four tested populations but still there are some separated lineages especially for Arabian and Thoroughbred horses. The sequences of 72 tested sequences were aligned with 13 published sequences as references, 11 of them for different Equus caballus whereas the other two reference sequences for Equus burchellii and Equus asinus. The results showed that all tested horses from the four populations are grouped with reference sequences of Equus caballus and separated from the other two reference sequences of Equus burchellii and Equus asinus.It is concluded that sequence analysis of mtDNA control region is still the most informative tool for the identification of genetic biodiversity and phylogeny of different horse breeds and populations. The horse populations reared in Egypt possess low genetic diversity and all of them are belonged to Equus caballus breed.

Diet Composition of the Large Herbivores in Mkambati Nature Reserve, Eastern Cape, South Africa

We used stable carbon isotopes from faeces to investigate the proportional contribution of C3 and C4 plant forms to the diet of the herbivores in Mkambati Nature Reserve, a grassland dominated ecosystem on the east coast of South Africa. Our results indicate that Equus burchellii, Damaliscus pygargus phillipsi, and Alcelaphus buselaphus utilize mainly C4 grasses. Tragelaphus oryx, Potamochoerus larvatus, Tragelaphus scriptus and Antidorcas marsupialis utilized mainly C3 plant forms but Redunca arundinum utilized an approximately equal amount of C3 and C4 plants. The results indicated similar trends compared to the more arid savanna systems (i.e. Kruger National Park in South Africa and several national parks in Kenya) with a few notable differences in some species (i.e. red hartebeest, southern reedbuck, eland, bushbuck and bushpig).

Keeper-Animal Interactions: Differences between the Behaviour of Zoo Animals Affect Stockmanship.

Stockmanship is a term used to describe the management of animals with a good stockperson someone who does this in a in a safe, effective, and low-stress manner for both the stock-keeper and animals involved. Although impacts of unfamiliar zoo visitors on animal behaviour have been extensively studied, the impact of stockmanship i.e familiar zoo keepers is a new area of research; which could reveal significant ramifications for zoo animal behaviour and welfare. It is likely that different relationships are formed dependant on the unique keeper-animal dyad (human-animal interaction, HAI). The aims of this study were to (1) investigate if unique keeper-animal dyads were formed in zoos, (2) determine whether keepers differed in their interactions towards animals regarding their attitude, animal knowledge and experience and (3) explore what factors affect keeper-animal dyads and ultimately influence animal behaviour and welfare. Eight black rhinoceros (Diceros bicornis), eleven Chapman’s zebra (Equus burchellii), and twelve Sulawesi crested black macaques (Macaca nigra) were studied in 6 zoos across the UK and USA. Subtle cues and commands directed by keepers towards animals were identified. The animals latency to respond and the respective behavioural response (cue-response) was recorded per keeper-animal dyad (n = 93). A questionnaire was constructed following a five-point Likert Scale design to record keeper demographic information and assess the job satisfaction of keepers, their attitude towards the animals and their perceived relationship with them. There was a significant difference in the animals’ latency to appropriately respond after cues and commands from different keepers, indicating unique keeper-animal dyads were formed. Stockmanship style was also different between keepers; two main components contributed equally towards this: “attitude towards the animals” and “knowledge and experience of the animals”. In this novel study, data demonstrated unique dyads were formed between keepers and zoo animals, which influenced animal behaviour.

Concordance on zebra stripes: a comment on Larison et al. (2015)

The functional significance of the extraordinary black and white stripes of zebras is still mysterious but now an active field of research. Four major hypotheses have been put forward: stripes are an antipredator defence operating through crypsis [1] or confusion of predators [2], are a means of reinforcing social bonds [3], are defence against ectoparasites [4] or are a means of cooling zebras [5]. Now, in the second multifactorial analysis of striping in zebras to date, Larison et al. [6] investigated the environmental factors that explain geographical variation in striping within a single species, the plains zebra ( Equus quagga or Equus burchellii ). They matched variation in striping patterns at 16 sites across its geographical range to a suite of environmental variables as well as tsetse fly (glossinid) distribution and lion ( Panthera leo ) presence. They found that greater intensities of intraspecific striping were associated with warmer temperatures and high precipitation. Last year we published a similar but phylogenetically controlled analysis using all seven species of equids, namely the three striped zebra species, the African wild ass ( Equus africanus ) which has leg stripes but no body stripes, and the unstriped species of equids ( E. hemionus, E. kiang, E. ferus przewalski ), and 20 of their subspecies, and compared several aspects of the four hypotheses [7]. These were camouflage in woodlands, antipredator defence against both lions and spotted hyaenas ( Crocuta crocuta ), social interactions, tsetse fly distribution, tabanid distribution using both temperature and humidity ranges as a proxy for abundance, and temperature isoclines to assess the cooling hypothesis. First we ran univariate tests on individual factors to identify the hypotheses with the strongest predictive ability, then subsequently used AICc model selection procedures to pit the most promising predictors against each other—a …

English

  This paper derives logistic models for Grant's gazelle Gazella granti, Thompson's gazelle(Gazella Thompsonii) and plains Zebra (Equus burchellii), in a game ranching system. The study was conducted on an 8100 hectares savannah ecosystem in Machakos district, with an average annual rainfall of 514±159.3 mm. Modelled as discrete-time logistic equations with fixed carrying capacities, it captures the wildlife herbivore population dynamics. Time series data, covering a period of 16 years, is used to generate the fixed carrying capacities and the interaction parameters endogenously. The estimation of the logistic models involves estimation of econometric models for each herbivore species, followed by the recovery of the carrying capacities, mathematically. The model-generated carrying capacities are 144.30 AU, 75.53 AU and 54.65 AU; the equivalent number of animals is 986, 929 and 144; for Grant’s gazelle, Thompson’s gazelle and Zebra, respectively. The derived “maximum sustainable yield” (MSY) stocking levels is 44 AU, 24 AU and 29 AU; the equivalent animal number is 297, 393 and 76; for Grant’s gazelle, Thompson’s gazelle and Zebra, respectively. Similarly, the MSY off-take level is 10.42 AU, 8.39 AU and 6.37 AU for these species respectively. These results show that the discrete-time logistic models are applicable to sustainable management of game ranching enterprise.   Key words: Carrying capacity, animal units, game ranching, species mix, logistic models, MSY.

Standing Sedation in Captive Zebra (Equus grevyiandEquus burchellii)

Nine Grevy's zebras (Equus grevyi) and three Burchell's zebras (Equus burchellii) were immobilized in a standing position a total of 70 times for minor, nonpainful procedures over a 9-yr period. Standing sedation was successfully obtained with a combination of detomidine and butorphanol on 47 occasions (67.1%). Detomidine i.m. (median 0.10 mg/kg; range: 0.07-0.21) was administered by dart, followed 10 min later by butorphanol i.m. (median 0.13 mg/kg; range 0.04-0.24). The dosages were varied depending on the initial demeanor of the animal. On 23 occasions (32.9%), small amounts of etorphine (median 2.5 microg/kg; range 1.1-12.3 microg/kg) plus acepromazine (median 10 microg/kg; range 4.4-50 microg/kg) (as in Large Animal-Immobilon) had to be administered i.m. to gain sufficient sedation. In these latter cases, the animals were either excited or known for their aggressive character. The zebras were sufficiently immobilized for the length of most procedures (<45 min) without supplementation. At the end of the procedure, the animals were given atipamezole (2 mg per 1 mg detomidine used) and naltrexone (0.1 mg/kg) to reverse the sedative effects, irrespective of whether etorphine was used or not. Standing sedation, using the combination of the alpha-2 agonist detomidine and the partial agonist-antagonist opioid butorphanol (in some cases supplemented with etorphine + acepromazine), proved to be a very efficacious and safe method to be used in zebras under zoo conditions for short-lasting, nonpainful procedures.

Social structure, vigilance and behaviour of plains zebra (Equus burchellii): a 5-year case study of individuals living on a managed wildlife reserve

Most studies of plains zebra (Equus burchellii) have focused on population ecology and have not included long-term observations of identified individuals. Over a 5-year period, we studied the crepuscular activities of 13 individual zebras within a focal group held within a managed game reserve. We also examined individual residency within the group by recording births, mortalities and longevity of group membership by adults. Residency of individuals living in other groups on the reserve was similarly monitored to examine variability in social structure within this closed population over an extended period of time. Stable, female groups were the mainstay of group sociality with male mean residency at 31.6 months being variable in length or even absent. Social interactions across all categories of zebras were free from aggression. Despite an absence of non-human predators, the proportion of dusk time budget allocated to vigilance was high, at 41% for males during periods when they accompanied stable female groups and 12% for females during these same periods. Female vigilance increased significantly to 19% when males were not resident. Females spent 70% of the time grazing and males just 36%. Due to its long-term nature, we concluded this study established a base line for plains zebra activity that could assist in understanding the factors that influence the successful management and conservation of healthy populations.

Further evidence for sex differences in suckling behaviour of captive plains zebra foals

Sex differences in suckling behaviour could be explained either by different maternal investment or by different behaviour of young. Previously, we found sex differences in rejection and termination of suckling bouts in captive plains zebras (Equus burchellii). In this study, we analysed the length of suckling bout interruptions and unsuccessful subsequent attempts which followed a successful suckling bout in plains zebra foals at Dvůr Kralove Zoo. When a suckling bout was interrupted, male foals resumed the bout sooner than female foals. The probability of a subsequent attempt decreased with increasing age in female foals but did not change in male foals. These results demonstrate higher effort in suckling interest by male foals, who tried to suckle more than female foals. Although we found sex bias in the effort to suckle, which suggested higher parent–offspring conflict in male offspring, we cannot omit a sex bias in milk supply by the mother.

A case of adoption and allonursing in captive plains zebra (Equus burchellii)

Although allonursing (allowing non-filial offspring to suckle) can be a costly behaviour, it has been reported for many mammals including ungulate species. However, such behaviour is very rare in equids. This is the first report on adoption and allonursing in captive plains zebra (Equus burchellii), recorded in the Dvůr Králové Zoo, Czech Republic. We observed a case of adoption of an orphaned foal by a lactating mare, who then regularly nursed two foals (filial and non-filial). The allonursing mare rejected more suckling attempts, terminated suckling bouts more often, and had a shorter suckling bout duration than other mares. When nursing both foals at the same time, the suckling bout lasted for less time than when nursing a single foal, regardless of whether it was filial or non-filial. The allonursing mare apparently did not discriminate between the filial and non-filial foal.

Feeding behaviour affects nursing behaviour in captive plains zebra ( Equus burchellii)

Equids spend more than half of the day feeding. Lactation is a very demanding form of maternal investment. In an environment such as a zoo, where no grass but several feeding sites are present, conflict between suckling behaviour of the foal and feeding behaviour of the lactating mother should occur. We observed 20 foals of captive plains zebra, Equus burchellii, at the Dvůr Králové Zoo, Czech Republic, and collected data concerning suckling events during 17 months of observation. First, we examined whether feeding by the mother while nursing affected suckling behaviour. We found that when the mother was feeding, the proportion of suckling bouts she terminated decreased with increasing age of the foal, whereas it did not change when she was not feeding. This result supported the trade-off between suckling and feeding behaviour which has been reported in other ungulates. Second, we examined what affected interruptions of feeding behaviour of the mother during the suckling bout. The proportion of interruptions of feeding by the mother during nursing increased with increasing age of her foal. This coincides with declining time spent nursing. In addition, younger mothers interrupted their feeding behaviour during suckling bouts more often than older ones. Mothers interrupted feeding during the suckling bout more often when they nursed a daughter than when they nursed a son. The results of our study show that feeding while suckling could reduce parent–offspring conflict and improve welfare of captive foals and mares.

Participatory wildlife surveys in communal lands: a case study from Simanjiro, Tanzania

AbstractIt is widely accepted that protected areas alone are not sufficient to conserve wildlife populations particularly for migratory or wide‐ranging species. In this study, we assess the population density of migratory species in the Tarangire–Simanjiro Ecosystem by conducting a ground census using DISTANCE sampling. We focus on the Simanjiro Plains which are used as a dispersal area by wildebeest (Connochaetes taurinus) and zebra (Equus burchellii). We demonstrate that DISTANCE sampling can provide precise estimates of population density and is an affordable method for monitoring wildlife populations over time. We stress the importance of involving local communities in monitoring programmes across landscapes that incorporate communal lands as well as protected areas.

Mother–offspring conflict in captive plains zebra (Equus burchellii): Suckling bout duration

Recently, a lack of any significant relationship between suckling bout duration and milk or energy intake has been revealed in several mammal species. However, a short suckling bout duration could be taken as an indicator of shortage of milk and lack of maternal care, and could therefore be used to test the parent–offspring conflict hypothesis. We examined suckling bout duration of 20 plains zebra (Equus burchellii) foals, at the Dvůr Králové Zoo, and collected data on suckling events during 19 months of observation. In total, we recorded 3252 suckling bouts during 831h of observation. As expected, suckling bout duration decreased with increasing age of the foal. The suckling bouts differed in duration when terminated by the foal, mother or any other adult mare (foal>mother>herdmate). Thus, bouts terminated by the mother should indicate that demands of the foal were not satisfied. The shortest bouts were those terminated by a herdmate; however, herdmates terminated few suckling bouts. Moreover, since the foal was able to resume suckling after interruption, the effect of a herdmate on suckling behaviour was very limited. Finally, we found that suckling bout duration was shorter in pregnant mothers than in non-pregnant ones, suggesting higher mother–offspring conflict when the mother was pregnant. In conclusion, this study demonstrates the importance of the termination of suckling bouts and the mother's pregnancy when interpreting suckling bout duration of equids, which has received little attention in previous studies. Our study showed that environmental condition can affect the suckling behaviour of captive equids.

Suckling behavior in captive plains zebra (Equus burchellii): Sex differences in foal behavior1

We predicted that the proportion of suckling attempts rejected and terminated by the mother would be greater for female foals than male foals, based on parent offspring conflict theory and on the assumption that throughout the study, all zebra mothers were in good condition because of captivity. We presumed that an increasing rate of suckling terminated or rejected by a mother would indicate a decreasing effort by the mother to invest in her offspring. We observed foals of captive plains zebras at the Dvůr Králové Zoo, Czech Republic. We found that the probability of successful suckling tended (slope = 0.0016; Z = 1.78; P = 0.074) to increase with increasing age of the female foals, but decreased (slope = -0.0018; Z = -2.51; P = 0.012) with increasing age of the male foals. The proportion of suckling bouts terminated by the mother decreased (slope = -0.0077; Z = -4.27; P < 0.0001) with increasing age of the female foals, but not the male foals (slope = -0.0005; Z = -0.34; P = 0.732). Our results indicate that conflict between mothers and female foals was less than that between mothers and male foals. The observed sex differences in termination and rejection of suckling bouts could be explained by the different behavior of the male and female foals, or by the selective maternal investment. Finally, we revealed no significant effect of herdmates on suckling behavior.

Lectin Histochemistry of the Glandular Part of the Gastric Mucosa of Zebra (Equus burchellii)

The Fundic glands of the stomach of Zebra were histochemically analyzed by conventional and lectin histochemistry. The surface mucous cells (SMC) and foveolar mucous cells (FMC) were highly rich with glycoprotein (GPs) with oxidizable vicinal diols and/or glycogen and GPs with carboxyl groups and/ or GPs with O-sulphate esters. The GPs with oxidezable vicinal diols and/or glycogen were predominates in FMC while, the GPs with carboxyel groups and/ or GPs with o-sulphate esters were predominate in SMC. The glandular epithelium of the base region of the fundic gland was devoid of both types of the GPs. All mucous secretig cells (SMC, FMC, mucous neck cells (MNC) and glandular mucous cells (GMS) were highly rich with α- anomer of the N-acetylgalacto-samine (αGalNAc), N- acetyl- glucosamine (GlcNAc) and neuroaminic acid (NeuNAc). The SMC and FMC were devoid of α- D-glucose (α- D- Glc), αD- mannose (α- D- Man), galac-Tose (Gal) and β- anomer of the Nacetylgalactosamine (β- GalNAc). The parietal cells (PC) appeared to react with Helix pomatia agglutinin (HPA), wheat germ agglutinine (WGA) and peanut agglutinin (PNA) indicating the existence of α- and β- anomer of the GalNAc and NeuNAc.

Mammalian body size changes and Plio-Pleistocene environmental shifts: implications for understanding hominin evolution in eastern and southern Africa

This study examines geographic and temporal variation in three mammalian taxa co-occurring in eastern and southern Africa. The selected taxa—the spotted hyena ( Crocuta crocuta), the plains zebra ( Equus burchellii), and the impala ( Aepyceros melampus)—are geographically widespread in modern times and are abundant in eastern and southern African Plio-Pleistocene fossil sites. Craniodental measurements of modern conspecifics from known geographic locations are compared using multivariate statistical methods to discern patterns of modern geographic variation within taxa. Modern and fossil samples are statistically compared to assess the nature and extent of inferred shifts in body size, both between modern samples and through time in each region. These results indicate that modern spotted hyenas and plains zebras exhibit mainly size variation between regions, with southern African samples possessing statistically larger craniodental metrics than eastern African samples. Comparison of fossil and modern samples reveals that the fossil assemblages do not show the same pattern of geographic variation. Significant temporal changes are more numerous between fossil and modern eastern African samples, and these changes are not mirrored by similar changes in the southern African samples. The changes experienced by taxa in eastern Africa appear to have been more extreme and wide-ranging than those in southern Africa, a presumed refugium. This result accords well with genetic studies of several large mammal species and paleoenvironmental studies suggesting that eastern African localized environments were more affected by tectonism and volcanism than were those in southern Africa. This study suggests that different evolutionary scenarios may have existed within Africa during the Plio-Pleistocene, but that both regions played unique and complementary roles in the evolution of African hominins and the broader faunal community.

A note on motor laterality in plains zebras (Equus burchellii) and impalas (Aepyceros melampus)

This observational field study used intermittent still photography to examine the distribution of motor laterality in images of two African grazing quadruped species: plains zebras (Equus burchellii) and impalas (Aepyceros melampus). Zebra images (n=708) had a laterality index of −6.21 and a Z-score of −1.65 (p=.098). Impala images (n=318) had a laterality index of −18.87 and a Z-score of −3.36 (p=.0008). The weak left motor bias in zebras aligns with that reported in domestic horses using a related method.

Diets of savanna ungulates from stable carbon isotope composition of faeces

AbstractHypotheses to explain diversity among African ungulates focus largely on niche separation along a browser/grazer continuum. However, a number of studies advocate that the browser/grazer distinction insufficiently describes the full extent of dietary variation that occurs within and between taxa. Disparate classification schemes exist because of a lack of uniform and reliable data for many taxa, and failure to incorporate spatio‐temporal variations into broader assessments of diet. In this study, we tested predictions for diet and dietary niche separation of African savanna ungulates using stable carbon isotope evidence from faeces for proportions of C3 (browse) to C4 (grass) intake among 19 species from the Kruger National Park, South Africa. Dietary predictions from the literature are confirmed in the case of browsers (black rhinoceros Diceros bicornis, giraffe Giraffa camelopardalis, bushbuck Tragelaphus scriptus, kudu Tragelaphus strepsiceros), mixed‐feeders (impala Aepyceros melampus, nyala Tragelaphus angasii), and most grazers (white rhinoceros Ceratotherium simum, Burchell's zebra Equus burchellii, warthog Phacochoerus africanus, hippopotamus Hippopotamus amphibius, blue wildebeest Connochaetes taurinus, tsessebe Damaliscus lunatus, waterbuck Kobus ellipsiprymnus). In contrast, several species showed results differing from most expectations derived from the available literature, including eland Taurotragus oryx, steenbok Raphicerus campestris, grey duiker Sylvicapra grimmia, buffalo Syncerus caffer, roan antelope Hippotragus equinus and sable antelope Hippotragus niger. Many of these discrepancies can be accounted for by seasonal and/or regional dietary differences. Cluster analysis based on a data matrix that incorporates the extent of spatio‐temporal dietary variation among Kruger Park ungulates reveals several distinct categories of feeding preferences that extend beyond a two‐edged browser/grazer dichotomy, such as mixed‐feeders with a preference for either forage class, and spatial/seasonal shifts between uniform and mixed‐feeding styles among variable browsers (e.g. grey duiker) and variable grazers (e.g. buffalo). These results highlight the need for approaches that are sensitive to spatio‐temporal variations and the continuity of diet.