Equisetum Arvense Research Articles

Effects of dynamised high dilutions and vegetal extract based on silicon on the growth and induction of resistance in tomato plants against Rhizoctonia solani

ABSTRACT The objective of this study was to study the response of tomato (Solanum lycopersicum) against Rhizoctonia solani using dynamised high dilutions and plant extract based on silicon. The treatments were Silicea terra (ST), Thuya occidentalis (TO), and Equisetum arvense (EA) at 30CH (Centesimal Hahnemannian dilution order), Equisetum arvense leaf extract (LE), and non-dynamised water as the control. Seeds of tomato cv Marmande were treated overnight. The in vitro bioassays involved assessment of R. solani mycelial growth, seed germination, and seedling development. In the greenhouse experiment, the emergence, root and aerial part development, and disease development were evaluated. Leaves were harvested to analyse concentrations of flavonoids, phenolic compounds, chlorophyll, carotenoids, and chitinase activity. In the in vitro bioassay, LE reduced mycelial growth and TO increased the seedling vigour index, dry weight, and length of seedlings. In the greenhouse experiment, TO and ST increased plant emergence. Tomato plants treated with ST improved the fresh weight and the length of the roots. The disease severity was decreased by ST, LE, and TO. The flavonoid concentration was higher in plants treated with TO and LE. Overall, the treatments of TO, EA, and ST improved the content of phenolic compounds. Moreover, endochitinase and chitobiosidase were activated by LE.

The antibacterial and antioxidant activities of combined Equisetum arvense extract with TiO2 nanoparticles in PMAA films

Studies on the antibacterial effects of natural compounds, particularly those derived from medicinal plants, have garnered a lot of attention because of the enhanced resistance of germs to antibiotics. The resistance of bacteria to plant-derived chemicals may develop much slower if not at all, which enhances the potential for future research in this area. This study investigates the antibacterial effect of Equisetum arvense extract combined with TiO2 nanoparticles and embedded in Polymethacrylic acid films on the growth of Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus. The prepared material was characterized via X-ray Diffraction, Scanning Electron Microscopy, and Atomic Force Microscopy to understand the physicochemical merits of it, and the antibacterial properties were then examined. The results indicate that Equisetum arvense exhibits antibacterial properties against Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. At modest dosages, this plant may offer a novel treatment option for disorders associated with oxidative stress, apoptosis, or hypertonic conditions.

The Antimicrobial and Antioxidant Effects of Equisetum arvense Extracts

Equisetum arvense L, also known as horsetail, is a medicinal plant used in traditional medicine. Especially, it is used in the treatment of bleeding, antiseptic, anti-inflammatory, urethritis, jaundice and hepatitis. In the study, the antimicrobial and antioxidant activities of extracts obtained from different solvents of E. arvense were investigated. Antimicrobial activity of E. arvense extracts was determined using the disc diffusion method. The antimicrobial activity was determined utilizing the pathogenic microorganisms Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, Candida albicans and Candida glabrata. In the results obtained, it was determined that the ethanol extract of E. arvense at 500 µg concentration showed antimicrobial activity at different rates (14.3-28.0). Ethanol extract showed the highest antimicrobial activity against Candida glabrata (28.0 mm) at the same concentration. It was detected that the chloroform extract showed antimicrobial activity (7.3-10.6 mm) against the microorganisms used. The antioxidant activity of the aerial parts of E. arvense at different concentrations of methanol extract was determined according to the 2,2-diphenyl-1-picrylhydrazil radical scavenging capacity method. The highest radical scavenging capacity of the methanol extract was observed at a concentration of 10mg/mL (91.5%). The IC50 value of the methanol extract of E. arvense was calculated as 3.13 mg/mL.

Bioactive Compounds Produced by Endophytic Bacteria and Their Plant Hosts-An Insight into the World of Chosen Herbaceous Ruderal Plants in Central Europe.

The natural environment has been significantly impacted by human activity, urbanization, and industrialization, leading to changes in living organisms and their adaptation to harsh conditions. Species, including plants, adapt to these changes by creating mechanisms and modifications that allow them to survive in harsh environments. Also, endophytes, microorganisms that live inside plants, can support plant growth and defense mechanisms in these conditions by synthesizing antimicrobial secondary metabolites. What is more, endophytes produce bioactive metabolites, including alkaloids, amines, and peptides, which play a crucial role in the relationship between endophytes and their host organisms. Endophytes themselves benefit from this by creating a stable environment for their survival and development. The aim of this review is to gain insight into endophytic bioactive metabolites from chosen synanthropic ruderal plants. Industrial activities release pollutants like heavy metals, by-products, and waste, which challenge living organisms and require adaptation. Synanthropic plants, where endophytes are abundant, are particularly valuable for their bioactive compounds, which are used in agriculture and medicine. This review presents, among others, endophytes of herbaceous ruderal plants from central Europe-Chelidonium majus L., Urtica dioica L., Plantago lanceolata L., Matricaria chamomilla L., Equisetum arvense L., Oenothera biennis L., Silybum marianum L., and Mentha piperita L.

Plant extracts and commercial products for controlling black spot and inducing resistance in rose bushes

Rose is one of the most appreciated flowers in the world; however, fungal diseases can affect its production, decreasing its commercial value. Thus, the objective of this work was to assess the potential of extracts of medicinal plants and commercial products based on plant extracts, plant oils, and potassium phosphite to control black spot (Diplocarpon rosae) in roses by evaluating the activation of plant defense mechanisms. The treatments consisted of using crude aqueous extract (CAE) of Rosmarinus officinalis leaves; CAE of Equisetum arvense stems; CAE of Moringa oleifera seeds; commercial product (CP) based on fermented plant extracts; CP based on potassium phosphite; CP based on plant oils; CP based on citrus mass; and fungicide. Severity of black spot disease, and yield, biometry, chlorophyll contents, and enzyme activity of peroxidase, catalase, and polyphenoloxidase in rose plants were evaluated. The treatments presented no difference from the control (water) for disease incidence and severity and yield. However, the longest stem was found in rose plants in the treatment with potassium phosphite-based commercial product, whereas the thickest stems were found in the three treatments with commercial products. The highest enzyme specific activities of peroxidase, catalase, and polyphenoloxidase were found in plants treated with potassium phosphite, CAE of M. oleifera, and CAE of R. officinalis. The results found showed that the tested natural products did not control black spot in rose bushes, despite their positive effect on plant physiology.

Ethanolic Extract of Equisetum arvense: A Potential Agent against Rheumatoid Arthritis in Wistar Rats with Freund’s Complete Adjuvant-Induced Arthritis

Ethanolic Extract of Equisetum arvense: A Potential Agent against Rheumatoid Arthritis in Wistar Rats with Freund’s Complete Adjuvant-Induced Arthritis

Different habitats of ferns growing in the Amonkoton stream, Zerafshan Valley, Uzbekistan

Abstract. Muminov D, Khaydarov K, Zhalov K, Hasanov M, Abdumuminova R, Ochilov U. 2024. Different habitats of ferns growing in the Amonkoton Stream (Zerafshan Valley), Uzbekistan. Biodiversitas 25: 2696-2704. The Zerafshan Valley, Uzbekistan, characterized by a warm and arid climate, has declined vegetation and soil quality over recent decades, marking a stark departure from its once lush past. Understanding the nuanced relationship between species and their habitat is imperative to unraveling this ecological transformation. This research delves into ferns, which are highly responsive to environmental factors, seeking to elucidate their circumstances within the changing ecosystem. Through strategically planned intensive transects and plots as proxies for fern growth and habitat analysis, statistical methods like the Shannon-Wiener Index (H) and Bray-Curtis index were deployed. The climate-related information analysis was conducted using the CRU TS 4.07 tool. We made use of average temperatures and precipitation over 120 years. The results of analyses have been acquired from 490 fern individuals belonging to 13 species, 7 genera, and 6 families. There are four species: Equisetum arvense L., Equisetum ramosissimum Desf., Cystopteris fragilis (L.) Bernh. and Adiantum capillus-veneris L. were employed as indicators of habitat. The findings underscore a substantial decline in fern-populated areas, resulting in diminished diversity and abundance and a relatively high rarity level. Furthermore, a latitudinal correlation with ferns along the stream direction is exhibited by four indicator species, offering valuable insights into the intricate dynamics of the Zerafshan Valley's ecosystem.

Magnetite Reduced Graphene Oxide/Ordered Mesoporous Carbon Nanocomposite as Effective Adsorbent for Removal of 2-Naphthol in Wastewater

Background: The wastewater released from various industries contains substantial amounts of organic compounds such as dyes and naphthols. However, naphthols are toxic to the environment and human health. So, it is essential to eliminate them, which will contribute to manufacturing and environmental management. Methods: In the work, an eco-friendly method is adapted to synthesize reduced graphene oxide (rGO) using Equisetum arvense plant extract as a strong reducing and stabilizing agent. Then, a hybrid nano adsorbent based on rGO and ordered mesoporous carbon (CMK-3) decorated with iron oxide nanoparticles (Fe3O4@rGO/CMK-3) was prepared as an adsorbent. We investigate the performance of Fe3O4@rGO/CMK-3 to remove 2-naphthol (2-NP). Results: The FE-SEM images exhibited spherical magnetite nanoparticles with sizes ranging from 31 to 47 nm on composite. Efficient removal (90%) of 2-NP from aqueous solution is demonstrated using high surface area Fe3O4@rGO/CMK-3 (initial concentration of 2-NP: 10 mg mL-1, pH: 5.0, time: 30 min, and amount of adsorbent dosage: 3 mg mL-1). The high surface area of Fe3O4@rGO/CMK-3, hydrogen binding, π-π stacking interaction between the benzene rings of 2-NP and graphitic skeleton of hybrid adsorbent facilitate the adsorption of 2-NP on the Fe3O4@rGO/CMK-3. The 2NP removal capacity by (Fe3O4@rGO/CMK-3) showed a significant decrease during five successive cycles. Conclusions: These results promise the potential of high surface area (Fe3O4@rGO/CMK-3) for efficient removal of 2-NP for wastewater treatment.

Equisetum arvense and nano zinc oxide‐infused polycaprolactone scaffolds: A multifaceted approach for antibacterial, antioxidant, and hemocompatible wound dressing

AbstractAn appropriate skin tissue‐engineered scaffold can act promptly to combat microbial infection and preserve the wound throughout all phases of healing. To achieve sufficient healing, scaffolds should also promote cell proliferation and regeneration. Therefore, it has become essential to develop skin tissue engineering scaffolds that mimic the extracellular matrices of the skin, provide a high porosity scaffold structure, and act as an antimicrobial protection during wound healing. In this study, the polycaprolactone (PCL) electrospun scaffold was modified with optimal amounts of zinc oxide nanoparticles (ZnO) and Equisetum arvense (EA) herbal extract for potential application as antibacterial and wound healing scaffolds. Full characterization was carried out for fabricated scaffolds before in‐vitro assessments. FTIR spectroscopy and SEM images verified the successful substitution of nanofibers with EA and ZnO. These substitutions increased the hydrophilicity compared to the PCL scaffold. According to the results, the PCL/ZnO/EA fabricated scaffold was hemocompatible and non‐cytotoxic, in addition to allowing proper cell attachment. Effective antibacterial efficiency against Gram‐positive and Gram‐negative bacteria was also observed in this sample. Finally, the in vitro wound healing assay indicated that the addition of ZnO and EA improves the wound healing capacity of the scaffolds. The results indicate the potential of the developed scaffolds to prevent wound contamination and the complications of chronic infection.Highlights Optimal concentrations of zinc oxide nanoparticles (ZnO) and Equisetum arvense (EA) were incorporated into electrospun polycaprolactone (PCL) nanofibers for potential wound dressing applications. The fabricated nanofibrous wound dressings offer a multitude of structural features in biomaterials. PCL fibers enable the controlled release of both ZnO and EA. The fabricated scaffolds exhibit favorable antibacterial, and antioxidant properties while maintaining biocompatibility and hemocompatibility.

Effect of common horsetail extract on growth characteristics, essential oil yield and chemical compositions of basil (Ocimum basilicum L.)

To investigate the effect of horsetail extract containing high silicon on morphological traits, growth, content, and compositions of essential oil of sweet basil (Ocimum basilicum L.) an experiment turned into carried out in the shape of a randomized complete block design with three replications. Foliar treatment of horsetail extract with zero, 0.5, 1, and 2% concentrations was applied on 6–8 leaf plants. The assessed traits include plant height, number of leaves per plant, number of sub-branches, leaf area index, plant fresh weight, plant dry weight, total anthocyanin, the content of total phenol and total flavonoid, antioxidant activity, essential oil content, and compounds were measured. The findings demonstrated that the increase of silicon-containing horsetail extract enhanced the improved increase in growth and phytochemical trait values. The use of horsetail extract in the 2% treatment increased plant height, the number of leaves per plant, the number of sub-branches, leaf area index, fresh weight, and dry weight of the plant by 49.79, 45.61, 91.09, 99.78, 52.78 and 109.25%, respectively, compared to the control. The highest content of total phenol (2.12 mg GAE/g DW), total flavonoid (1.73 mg RE/g DW), total anthocyanin (0.83 mg C3G/g DW), and antioxidant activity (184.3 µg/ml) was observed in the 2% extract treatment. The content of essential oil increased with increasing the concentration of horsetail extract, so the highest amount of essential oil was obtained at the concentration of 2%, which increased by 134.78% compared to the control. By using GC–MS, the essential oil was analyzed. The main components of the essential oil include methyl eugenol (12.93–25.93%), eugenol (17.63–27.51%), 1,8-cineole (15.63–20.84%), linalool (8.31–19.63%) and (Z)-caryophyllene (6.02–14.93%). Increasing the concentration of horsetail extract increased the compounds of eugenol, 1,8-cineole, and linalool in essential oil compared to the control, but decreased the compounds of methyl eugenol and (Z)-caryophyllene. Foliar spraying of horsetail extract, which contains high amounts of silicon, as a stimulant and biological fertilizer, can be a beneficial ingredient in increasing the yield and production of medicinal plants, especially in organic essential oil production.

Phytochemical screening and antimicrobial activity of some medicinal plants

The antimicrobial activity of the aqueous and ethanolic extracts of Myrtus communis, Ammi visnaga, and Equisetum arvense was investigated against Staphylococcus aureus, Escherichia coli, and Candida albicans by using the agar well diffusion method. Serial concentrations (15%, 30%, and 50%) of the extracts of each plant were tested and compared with gentamicin (10 μg) and fluconazole (25 μg). Most of the extract concentrations showed a relatively high antimicrobial activity against all the tested microbes, and the ethanolic extract was more effective than the aque¬ous extract. The activity of plant extracts increased with the increasing extract concentration of Myrtus communis, which appeared to possess a more antimicrobial activity than the other plants assessed; in fact, its ethanolic extract exhibited the highest inhibition zone against S. aureus (32 mm). The ethanolic plant extracts at a concentration of 50% displayed the maximum activity against the herein assessed isolates. Moreover, E. coli showed a higher sensi¬tivity to most extracts, while the lowest effect being noticed on C. albicans.

Acaricidal Efficacy of Thirty-Five Egyptian Plants Against the Camel Tick, Hyalomma Dromedarii.

Alternative and affordable tick control strategies are crucial to control and prevent tick bites and tick-borne diseases. In this study, we evaluated the acaricidal efficacy of 35 aqueous plant extracts (17%) against the camel tick, Hyalomma dromedarii. The phytochemical profile indicated the presence of various secondary substances. Plants were classified into three groups according to their mortality percentage 15days post-treatment with 17%. This highly effective group (91%-95%) comprised Ocimum basilicum, Mespilus germanica, and Viola alpine followed by Carum carvi, Cucurbita pepo (peel), and Peganum harmala. A moderately effective group (80%-90%) included Acacia nilotica, Apium graveolens, Capsicum annuum, Ceratonia siliqua, Cucurbita pepo (seeds), Equisetum arvense, Eruca sativa, Ginkgobiloba, Plantagopsyllium, Phyllanthusemblica, Punica granatum, and Ziziphus spinachristi. The 20 remaining plants were assigned to the less effective group (< 80%). Viscum album (58.3%), which was the least effectivereference plant. The high potency of six plant extracts as acaricides may be attributed to the high content of active principles, e.g., phenols, flavonoids, and tannins. All of these highly effective plants are recommended for use as an acaricide, in case offacing acaricidal resistance or limited options for tick control.

Equisetum arvense as a silica fertilizer

The aim was to use the agricultural weed and silica (Si) hyperaccumulator Equisetum arvense as Si fertilizer in plant cultivation. We investigated (1) the Si uptake in various Equisetum species, (2) where Si accumulates in the Equisetum plant, (3) processing methods to release as much Si as possible from dried, ground E. arvense plants and (4) which treatment yields gives the highest uptake of Si in young wheat plants cultivated in soil containing ground E. arvense. The results showed that E. arvense containes 22% Si and was among the best Si accumulators. Equisetum arvense accumulates Si as both soluble and firmly bound fractions. Amorphous silica (SiO2) accumulates in the outer cell walls of epidermis of the entire plant. Regarding the processing method, a longer treatment time, greater concentration of Equisetum, boiling, and the addition of sodium bicarbonate increased the Si availability in ground, dried E. arvense. The addition of untreated, ground, dried E. arvense to the soil, corresponding to 160 kg Si ha−1, increased the available Si in the soil and the Si uptake in wheat plants by five-fold, compared with the control. Boiling the ground E. arvense increased the Si uptake by 10 times, and the of sodium bicarbonate increased the availability and uptake by 40 times, compared with the control. In conclusion, dried, ground E. arvense can be used as a Si fertilizer as is, after boiling for a slightly better effect, or with sodium bicarbonate (up to a similar amount as the ground material) for best effect.

Equisetum arvense standardized dried extract hinders age-related osteosarcopenia

Age-associated osteosarcopenia is an unresolved syndrome characterized by the concomitant loss of bone (osteopenia) and skeletal muscle (sarcopenia) tissues increasing falls, immobility, morbidity, and mortality. Unbalanced resorption of bone in the remodeling process and excessive protein breakdown, especially fast type II myosin heavy chain (MyHC-II) isoform and myofiber metabolic shift, are the leading causes of bone and muscle deterioration in the elderly, respectively. Equisetum arvense (EQ) is a plant traditionally recommended for many pathological conditions due to its anti-inflammatory properties. Thus, considering that a chronic low-grade inflammatory state predisposes to both osteoporosis and sarcopenia, we tested a standardized hydroalcoholic extract of EQ in in vitro models of muscle atrophy [C2C12 myotubes treated with proinflammatory cytokines (TNFα/IFNγ), excess glucocorticoids (dexamethasone), or the osteokine, receptor activator of nuclear factor kappa-B ligand (RANKL)] and osteoclastogenesis (RAW 264.7 cells treated with RANKL). We found that EQ counteracted myotube atrophy, blunting the activity of several pathways depending on the applied stimulus, and reduced osteoclast formation and activity. By in silico target fishing, IKKB-dependent nuclear factor kappa-B (NF-κB) inhibition emerges as a potential common mechanism underlying EQ′s anti-atrophic effects. Consumption of EQ (500 mg/kg/day) by pre-geriatric C57BL/6 mice for 3 months translated into: i) maintenance of muscle mass and performance; ii) restrained myofiber oxidative shift; iii) slowed down age-related modifications in osteoporotic bone, significantly preserving trabecular connectivity density; iv) reduced muscle- and spleen-related inflammation. EQ can preserve muscle functionality and bone remodeling during aging, potentially valuable as a natural treatment for osteosarcopenia.

Optimizing the ultrasound-assisted phenolic extraction from Equisetum arvense L. and its antioxidant activity using response surface methodology

Optimizing the ultrasound-assisted phenolic extraction from Equisetum arvense L. and its antioxidant activity using response surface methodology

МОДИФИКАЦИЯ МЕТОДИКИ КОЛИЧЕСТВЕННОГО ОПРЕДЕЛЕНИЯ ФЛАВОНОИДОВ В ТРАВЕ ХВОЩА ПОЛЕВОГО (EQUISETUM ARVENSE L.)

В настоящей работе представлены варианты модификации методики стандартизации лекарственного растительного сырья хвоща полевого (Equisetum arvense L.). Целью исследования являлось установление оптимальных условий, времени и метода экстракции для получения максимального выхода суммы флавоноидов в сырье в пересчете на кверцетин. Метод количественного анализа травы хвоща полевого основан на использовании метода спектрофотометрии при аналитической длине волны 430 нм, в пересчете на кверцетин. Извлечение биологически активных веществ осуществляли на кипящей водяной бане, в течение 60 минут, в соотношении (сырье : экстрагент – 1:100), концентрация спирта составила 50 %. Данные условия экстракции позволили получить максимальный выход флавоноидов, который составил 1,67 ± 0,07 %. В ходе проведения эксперимента при экстрагировании был дополнительно использован ультразвук в сочетании с водяной баней, однако выход флавоноидов был существенно меньше, соответственно, следует вывод о разрушительном воздействии ультразвука на флавоноиды в траве хвоща полевого. Предложенные нами условия экстрагирования в дальнейшем можно рекомендовать для получения экстрактов с целью изготовления лекарственных препаратов на основе сырья травы хвоща полевого. This paper presents options for modifying the methodology of standardization of medicinal plant raw materials of horsetail (Equisetum arvense L.). The purpose of the study was to establish optimal conditions, time and extraction method to obtain the maximum yield of the amount of flavonoids in raw materials calculated on quercetin. The method of quantitative analysis of horsetail herbs is based on the use of spectrophotometry at an analytical wavelength of 430 nm, calculated on quercetin. The extraction of biologically active substances was carried out in a boiling water bath, for 60 minutes, in the ratio (raw materials:extractant 1:100), the alcohol concentration was 50%. These extraction conditions allowed to obtain the maximum yield of flavonoids, which was 1.67±0,07%. During the experiment, ultrasound was additionally used during extraction, in combination with a water bath, but the yield of flavonoids was significantly less, respectively, the conclusion follows about the destructive effect of ultrasound on flavonoids in horsetail herbs. The extraction conditions proposed by us can be further recommended for obtaining extracts for the purpose of manufacturing medicines based on the raw materials of horsetail herbs.

The effect of copper reduction on the control of downy mildew in Mediterranean grapevines

The application of copper-based fungicides has led to the problematic accumulation of copper in the soil of many European vineyards. In this work, we tested the effectiveness of downy mildew control strategies that considerably reduce the amount of copper, through the application of other non-toxic compounds. The study took place in three different regions of Catalonia, by combining smaller and larger-scale trials for two growing seasons. Although variations among experiments were detected, the treatments used here may reduce the applied copper content by up to 77 percent. The cooper reduction-strategies, which alternated standard copper application of copper oxychloride with applications of products based on Equisetum arvense L., or with applications of products with heptagluconic acid (an alternative formulation with a very low-rate of copper), provided the most consistent protection against downy mildew. Moreover, these treatments reduced copper inputs by 63 and 47 percent as compared to conventional treatments based on repeated applications of copper oxychloride. This work underlines the possibility of reducing the amount of copper applied to control downy mildew in Mediterranean vineyards, through the use of newly-developed formulations.

Histological Evaluation of Local Application of Equisetum Arvense /Urtica Dioica Extract on Bone Healing in Rabbits

Histological Evaluation of Local Application of Equisetum Arvense /Urtica Dioica Extract on Bone Healing in Rabbits

Evaluation of the Anti-inflammatory Activity of Equisetum arvense and Baccharis trimera Fractions

Inflammation is a natural response of the body to defend itself against potential threats and can be reduced through physical activity, proper nutrition, and the use of herbal medicines, which are medicinal plants. In the study, we aim to examine the anti-inflammatory effects of the volatile and ethanolic fractions of two commonly used medicinal plants, Equisetum arvense, and Baccharis trimera. The essential oils were obtained by hydrodistillation of the fresh leaves of the plants, while the ethanolic extracts were obtained using classical methodologies. All fractions were tested for anti-inflammatory activity, evaluating their ability to stabilize the red blood cell membrane and inhibit the spreading, and phagocytosis by macrophages, at concentrations varying from 200 to 600 µg mL-1. The results of the experiments suggest that the ethanolic fraction of B. trimera shows promising results compared to the positive controls. Our investigations thus contribute to the specialized literature on the use of herbal medicines around nutrition, providing guidance for future studies on these fractions.

The presence of radiotoxic 210Po and 210Pb in Ukrainian wild medicinal plants and the assessment of related dose and cancer risk.

Herbal plants are considered natural pharmaceuticals but also accumulate chemical elements and compounds at high levels. 210Po and 210Pb are highly radiotoxic and may cause carcinogenesis due to ionizing radiation. Thirteen of the most popular wild-growing herbaceous plant species, mostly included in the European Pharmacopoeia, were collected across Ukraine. 210Po and 210Pb were determined in the medicinal plants, and their radiotoxicity and cancer risk were calculated. The results of 210Po activities measured in herbal raw material ranged from 2.28Bq kg-1 dw (where dw is dry weight) in herb of common horsetail (Equisetum arvense L.) collected near Lviv to 37.7Bq kg-1 dw in leaves of common birch (Betula pendula Roth.) from Rava Ruska. The activity concentrations of 210Pb varied from 0.44Bq kg-1 dw in leaves of common birch from Starokostyantyniv to 28.4Bq kg-1 dw in leaves of common birch from Mizhhirya. There were statistically significant differences between 210Po and 210Pb content in flowers, leaves, and aerial portions. The studies indicated that the estimated annual effective radiation dose from Ukrainian herbs consumption was low, ranging 5.00-82.6 µSv from 210Po and 0.56-35.8 µSv from 210Pb, while the cancer morbidity and mortality risk ranged from 10-4 to 10-6. The presented data indicated no radiological risk related to using herbal plant materials.