Background: In Iraq, breast tumor is the greatest prevalent form of female malignancy. Increasing research in the past ten years shows that many human malignancies, including breast tumors, are associated with Human Cytomegalovirus and high-risk Human Papilloma Virus. Objectives: The aim of this research was to determine the role of HPV and HCMV in benign and malignant breast tumors. Methods: This retrospective research used 140 chosen formalin-fixed, paraffin-embedded pieces of breast specimen taken from 100 patients of breast tumor (70 cases of ductal mammary, 15 patients of lobular cancer, five persons mixed from ductal and lobular cancer and ten persons of medullary cancer) and 20 patients infected with benign epithelial lesion As well as 20 pieces of normal breast tissue as control groups. The range of age was 16-72 years. The study was conducted in hospitals and private laboratories in Kirkuk and Tikrit city from 15th January 2022 to 15th December 2022 using the in situ hybridization (ISH) technique. Results: The archival samples collected were related to breast cancer patients ranging from 16 years to 70 years of age, with a maximum age of 73 years in breast cancer and 60 years in healthy women. ISH found that CMV was positive in (25 percent) breast cancer tissues (25 / 100) according to the control group were negative. While in HPV demonstrated (45%) 45/ 100 breast cancer tissues compared to the control group were negative. Of these, The findings revealed that the spreading of HPV-DNA genotypes in malignant and benign cancer groups were as follows: HPV16 10 (20.8%), HPV 18 10 (20.8%), and HPV 31 28 (58.33%). These results suggest that HPV genotype 31 appeared at a greater rate than other genotypes (16 and 18). In 25 (55.6 percent) cases, the largest ratio of co-infection with several high-risk genotypes of HPV 16 + 18 + 31 was observed. Co-infection in the malignant group with other high-danger HPV genotypes was as follows: HPV 16 +18 6(13.33%), HPV 16 + 31 6(13.33), and HPV 18+31 8(17.8%). Positive signs suggesting high-risk HPV infection were detected for 1/1 (100 percent) of benign breast tumor tissues; 30(66.7 percent) mixed HPV infection was found in 45 cases of malignant breast tumors. Conclusion: The results of this study showed a connection between HCMV and high-oncogenic genotypes of HPV in breast cancer patients.