Episodes Of High Temperature Research Articles

Comparative proteomics in tall fescue to reveal underlying mechanisms for improving Photosystem II thermotolerance during heat stress memory

BackgroundThe escalating impacts of global warming intensify the detrimental effects of heat stress on crop growth and yield. Among the earliest and most vulnerable sites of damage is Photosystem II (PSII). Plants exposed to recurring high temperatures develop heat stress memory, a phenomenon that enables them to retain information from previous stress events to better cope with subsequent one. Understanding the components and regulatory networks associated with heat stress memory is crucial for the development of heat-resistant crops.ResultsPhysiological assays revealed that heat priming (HP) enabled tall fescue to possess higher Photosystem II photochemical activity when subjected to trigger stress. To investigate the underlying mechanisms of heat stress memory, we performed comparative proteomic analyses on tall fescue leaves at S0 (control), R4 (primed), and S5 (triggering), using an integrated approach of Tandem Mass Tag (TMT) labeling and Liquid Chromatography-Mass Spectrometry. A total of 3,851 proteins were detected, with quantitative information available for 3,835 proteins. Among these, we identified 1,423 differentially abundant proteins (DAPs), including 526 proteins that were classified as Heat Stress Memory Proteins (HSMPs). GO and KEGG enrichment analyses revealed that the HSMPs were primarily associated with the “autophagy” in R4 and with “PSII repair”, “HSP binding”, and “peptidase activity” in S5. Notably, we identified 7 chloroplast-localized HSMPs (HSP21, DJC77, EGY3, LHCA4, LQY1, PSBR and DEGP8, R4/S0 > 1.2, S5/S0 > 1.2), which were considered to be effectors linked to PSII heat stress memory, predominantly in cluster 4. Protein-protein interaction (PPI) analysis indicated that the ubiquitin-proteasome system, with key nodes at UPL3, RAD23b, and UCH3, might play a role in the selective retention of memory effectors in the R4 stage. Furthermore, we conducted RT-qPCR validation on 12 genes, and the results showed that in comparison to the S5 stage, the R4 stage exhibited reduced consistency between transcript and protein levels, providing additional evidence for post-transcriptional regulation in R4.ConclusionsThese findings provide valuable insights into the establishment of heat stress memory under recurring high-temperature episodes and offer a conceptual framework for breeding thermotolerant crops with improved PSII functionality.

Citrullus colocynthis regulates photosynthetic and biochemical processes to develop stress resilience and sustain growth under sub-optimal temperatures

Sub-optimal temperatures posed significant challenges to leaf photosynthesis and plant growth. This study examined the eco-physiological adaptations of a desert vine (Citrullus colocynthis) to unfavorable temperatures (15/25 °C, 25/35 °C, and 35/45 °C) over 35 days. Plants grown in moderate temperatures increased shoot number and leaf canopy (55 %), chlorophyll a (2.5 folds), and carotenoid accumulation compared to other treatments. Conversely, high-temperature stress resulted in longer shoot development with fewer primary branches, while low temperatures constrained both shoot and leaf growth. Plants treated with high heat exposure produced more oxidative stress markers (MDA and H2O2, by two folds), causing greater cellular damage while attaining lesser growth. During the high-temperature episode, plants increased their antioxidant enzyme activities and proline (2.5-fold) levels to protect the tissues from oxidative damage. Plants treated with either moderate or higher heat stress increased the potential quantum yield of photosystem II (Fv/Fm) and maximum quantum yield ФPSII in the young leaves, which indicated the constitutive plant adaptation to unfavorable temperatures. Additionally, photosynthetic efficiency differed with leaf age and temperature; mature leaves performed better physiologically at low temperatures, whereas young leaves adapted better at high temperatures stress. The results suggested that C. colocynthis plants were highly adaptable to unfavorable temperatures by regulating the plants’ thermal homeostatic ability through their photosynthetic and biochemical processes. This study provided deeper insights into species-specific eco-physiological responses to sub-optimal temperatures and contributed to our broader understanding of plant resilience and ecological adaptability in the era of global climate change.

Contrasting Responses of Spring and Summer Potato to Climate Change in South Korea

This paper assessed the effects of climate change and planting date adjustment on spring and summer potato in South Korea for the period 2061–2090. The study applied the SUBSTOR-Potato model and outputs of 24 general circulation models to capture future variability in climate conditions for four shared socioeconomic pathway-representative concentration pathway scenarios. Without planting date adjustment, tuber yield was projected to increase by approximately 20% for spring and summer potato, indicating that the CO2 fertilization effect would offset the adverse effect of rising temperature. The effect of planting date adjustment was significant only for spring potato, where overall climate change impact with the optimized planting date was approximately +60%. For spring potato, the effects of rising temperature were bidirectional: temperature increases early in the year extended the growing season, whereas the higher temperature increases in June under the most severe climate change condition accelerated leaf senescence and reduced tuber bulking rate. Based on these results, different adaptation strategies could be established for spring potato for different climate change conditions. For example, developing frost-tolerant cultivars would continue to be recommended to plant earlier under the mild climate change conditions, whereas breeding mid-late maturity cultivars with high-temperature tolerance would be needed to delay senescence and enhance late tuber growth under the severe climate change conditions. Unlike spring potato, the breeding goal for summer potato of increasing high-temperature tolerance holds across all climate change conditions. Finally, these optimistic results should be interpreted with caution as the current model does not fully capture the effect of high-temperature episodes and the interactive effect between CO2 and temperature, which may reduce beneficial projected climate change impacts.

Water replenishment to maize under heat stress improves canopy temperature and grain filling traits during the reproductive stage

The occurrence of heat often exacerbates drought stress by increasing atmospheric vapor pressure deficit and decreasing transpiration efficiency. Water replenishment to increase soil water content (SWC) could be an effective measure for improving crop resilience in heat. However, field observational evidence and the underlying mechanism for the mitigation were still limited. For this, two widely adopted maize hybrids (ZD958 and XY335) were planted in 2019 and 2020 in North China Plain with three growth environments during the reproductive stage: Normal field conditions (NC), High-temperature episodes (HT) and High-temperature together with elevating SWC (HT-W). Averagely, high temperature reduced grain yield by 21.6-65.1% in HT treatment compared with NC treatment. In HT-W treatment, grain yield was improved by 11.8-13.5% while SWC was elevated by 27.6–51.5% in 0–80 cm soil profile compared with HT treatment. In HT-W treatment, water replenishment to maize under heat stress improved canopy temperature and grain filling traits. The daily maximum canopy temperature was reduced by 1.0–3.1 °C. The effective grain-filling period (Pf) was extended by 1.0–1.6 days and the mean grain-filling rate (Gmean) was increased by 8.2%-10.5%. The improvement of Gmean and Pf for ZD958 was greater than XY335. Furthermore, the significant correlations between SWC in 0–20 cm soil profile and Gmean, and between SWC in 40–80 cm soil profile and Pf for both hybrids were observed. As a result, the fresh weight, volume, and water content of the grain were significantly improved in HT-W treatment compared with HT treatment. Overall, per unit increase in SWC (0–80 cm) decreased the daily maximum canopy temperature by 0.3 °C, increased dry matter accumulation by 1.5%, and grain yield by 2.1%. The information obtained from this study will be helpful to develop efficient management to protect against heat stress in the field.

AMMI biplot analysis for stability in early maturity group of rice (Oryza sativa L.)

Recent predictions on climate change indicate that episodes of unseasonal rains, cold stress, and high temperatures are expected to impact rice production and productivity. To obtain consistent yield across diverse environments, a rice variety should have adaptability and stability to fit into various growing seasons and locations. In the present investigation, AMMI model was employed to assess the stability of nine rice genotypes of the early maturity group across two summer and rainy seasons. Combined analysis of variance expressed a significant genotype, environment and genotype × environment interaction for grain yield and days to 50% flowering. The study also suggested environmental effect as the greatest part of the variation, followed by genotypic and genotype × environment interaction effects for these traits. Biplots and statistics of AMMI identified that G1, G3 and G5 were the most stable and adapted high yielding rice genotypes, while G9, G4 and G6 appeared to be the most stable genotypes with earliness. Hence, these genotypes could be used as directly as varieties or as donors in future breeding programmes for improving rice productivity in the early maturity group after evaluation under multi-location trials.

Early Mylonitization in the Nevado-Filábride Complex (Betic Cordillera) during the High-Pressure Episode: Petrological, Geochemical and Thermobarometric Data

In the western part of the Sierra de los Filabres area, there are fine-grained metamorphic rock bands, showing a field aspect simitar to slates, as previously described in the geological literature of the studied region. They are variable in thickness, from millimeters to tens of meters and appear intercalated in the schist succession. The geochemical resemblance between both types of rocks (major, minor and trace elements), determined by a statistical approach and the comparison of depositional condition indices, points to a similar sedimentary origin of the protolith but different clay content. Mineral facies and illite “crystallinity” indices in the so-called slates indicate that they followed the same metamorphic path and reached the same metamorphic grade than schists. According to compositional zoning detected in micas and garnets present in both lithologies and the P-T conditions deduced from garnets, the mineral nucleation and growth episode of the main mineral paragenesis in these fine-grained schists was more remarkable during the high-pressure event, with a no significant effect of the latter low-pressure–high-temperature episode. In contrast, the coarse-grained schists developed higher size minerals during the low-pressure–high-temperature episode. A differential mylonitization process during the metamorphism is proposed to justify the discrepant field appearance and the contrasting response of both types of metapelitic rocks to the latter metamorphic event.

Effects of Short-Term High Temperature on Gas Exchange in Kiwifruits (Actinidia spp.).

Climate changes occurring today require detailed research of the effects of increasing temperatures on photosynthesis in different species and cultivars. Temperature variability is a crucial determinant of the yield and quality of plants, particularly when high-temperature episodes coincide with their growth and fruit development. The effect of high temperature (HT) on higher plants primarily concerns photosynthetic functions, but the sensitivity of photosynthesis to high temperature is not well-understood in kiwifruits. In this study, we designed a new environmental monitoring system to evaluate the effects of environmental factors on the photosynthetic physiology of different kiwifruit species and cultivars. A significant positive correlation was determined between the main photosynthetic indices of kiwifruits, such as transpiration rate and net photosynthetic rate. The net photosynthetic rate of commercial kiwifruit cultivars was strongly inhibited when the temperature exceeded 44.5 °C, and the leaves of kiwifruits were irreversibly damaged when the temperature increased to 52 °C. Kiwifruit cultivars with different ploidy levels (diploid, tetraploid and hexaploid) were found to be sensitive to high temperature, whereas tetraploids had higher HT resistance and hexaploids had the highest net photosynthetic rate. Further research showed that the HT tolerance of kiwifruits existed not only between species but also among cultivars. A. eriantha had the highest net photosynthetic rate at more than 44.7 °C, but those of A. deliciosa and A. arguta declined sharply as the temperature exceeded 43.5 °C. As a result, it was determined that high temperatures have important effects on the photosynthetic activities of kiwifruit plants with different ploidy levels, and that these effects can significantly change their development according to how they differ among different species/cultivars.

Monitoring of high temperature weather episodes with satellite data

INSAT-3D/3R Geosatellites are in orbit at 74 and 82 °E positins oerating in staggring mode of 15 minute temporal frequency providing contineous coverage in high spatial domain throgh 6 channel Imager. Satellite derived geophysical parameters like Outgoing Long wave Radiation (OLR), Land Surface Temperature (LST) and Upper Trpospheric Humidity etc are found to very useful in monitoring the high temperature weather episodes affecting almost entie country like Punjab, Hariyam Rajastham Uttar Pradesh (UP), Bihar, Madhra Pradesh (MP)P, , Maharastra, Andhra Paradesh (AP) , Telengana, Tamilnadu Orissa etc starting from mid of March-2022. The actual temperatures in the above said areas pesrsisting above normal ( ~ 4 to 7 C) from Mid March to Mid April-2022 and caued heat wave conditiions in most of the places of the country. The persisteny of the prevaling heat wave conditions is not only related to surface features but also upper air anticyclonic circulations and sinking of dry air over the affected areas. The entire feedback cycle from lower to middle ttroposphere operating with dry air mass (Northerly) almost entire Northwest , Central and parts of South (Maharastra, AP and Telengana) and keep the areas warm and above normal temperature range. The eastward moving systems also not reaching to lower northern latutudes and moving away and keeping away misture laden winds.
 This paper brought out the role of INSAT-3D/3R satellite derived products in monitoring such high temperature episodes. Such type of weather events blended with the support of climatologial features of prevailing weather systems of the season can be utilized in forecasting and its persistence over the area. It is seen from the study that, the OLR vales threshold ranges from 330 -380 watt /m2 in pockets of warmer temperatures or suffering from heat wave conditions. Similarly, the Land surface temperature and UTH values 306 -325 °K & 05-25 % respectively. The IPWV values in mm are also varies from 2-12 mm during mid-March to mid-April-2022.
 
 The satellite and climatological information can be clubbed together up to village level to help the society and disaster management authorities at state /district level in advance of such high impact weather events. This study is done with limited data sets and in future, it will be carrid out for larger doimain and a suitable the long period avarage (LPA) of past INSAT data (daily, monthly, seasonal), so that areawise thresholds can be generated from almost for entire Indian domain.

Slow cooling during crystallisation of barred olivine chondrules

Barred olivine (BO) chondrules are small ferromagnesian silicate igneous droplets with unique dendritic textures that are considered to have formed in the early solar system during one or more brief high-temperature episodes, followed by rapid cooling in a gas. Rapid cooling rates of 100–7200 °C/h during chondrule formation have been proposed based on experiments attempting to reproduce BO crystal textures. However, the BO texture has never truly been reproduced under such rapid cooling conditions. Here, we experimentally show that true BO textures can be produced either after rapid cooling (>50 °C/h) following by reheating step or by cooling rates slower than 10 °C/h. Regardless of the thermal history considered, the chemical compositions of glass inclusions trapped within olivines of BO chondrules imply a final slow cooling rate one to two orders of magnitude below previous estimates. Such slow cooling rates are consistent with those estimated for plagioclase-bearing porphyritic chondrules and magmatic type-B Ca-Al-rich inclusions, suggesting that slow cooling rates are common to all similar chondritic objects.

Canopy cooling traits associated with yield performance in heat-stressed oat

In the upper U.S. Midwest, oat is a promising crop for diversifying local cropping systems and providing local consumers with a healthy food option. However, as a result of climate change, the crop is facing increasingly severe episodes of high temperature (HT) stress during reproductive development, which limit its yield potential. The goal of this 3-year field study was to identify locally-adapted HT stress-tolerant oat breeding lines, propose a parsimonious mechanistic basis for HT stress tolerance and leverage it to develop a screening method with the potential to support a breeding program. Because water supply is rarely limiting in the region, we tested the hypothesis that canopy latent cooling would be a desirable trait. To this end, we deployed “heat tents” on a core panel of 30 oat lines between booting and heading, where we measured i) grain yield and quality traits (protein, oil and beta-glucan), ii) pollen viability, percentage of filled florets, and grain number, iii) gas exchange and canopy cooling parameters (e.g., photosynthesis, transpiration rate, stomatal conductance, canopy temperature depression). Additionally, we developed a thermal imaging approach to remotely quantify canopy temperature on contrasted lines. Significant genotypic variability was detected in yield and quality trait responses to the HT stress and a physiological path model identified canopy cooling as a main process explaining superior yield performance of HT stress tolerant lines. Interestingly, pollen viability was not found to be the main driver of yield declines, suggesting a role played by other reproductive processes, including those underlying female tissue/organs sensitivity to HT stress. Finally, canopy cooling parameters correlated with geographic locations of breeding programs from which the tested germplasm originated, indicating that they are under selection. Overall, this study opens the way for a breeding program targeting the development of more climate-resilient oats adapted to northern climates.

Metabonomic Analysis Provides New Insights into the Response of Zhikong Scallop (Chlamys farreri) to Heat Stress by Improving Energy Metabolism and Antioxidant Capacity.

Temperature is an important factor affecting the growth, development and survival of marine organisms. A short episode of high temperature has been proven to be a severe threat to sustainable shellfish culture. Zhikong scallop (Chlamys farreri), a shellfish with broad economic and biological value in North China, has frequently experienced heat stress in summer in recent years. To understand the effects of heat stress on shellfish, the metabolism of C. farreri was analyzed after exposure to 27 °C for either 6 h or 30 d. After 6 h of heat stress exposure, a total of 326 and 264 significantly different metabolites (SDMs) were identified in gill and mantle tissues, respectively. After 30 d of heat stress exposure, a total of 381 and 341 SDMs were found in the gill and mantle tissues, respectively. These SDMs were mainly related to the metabolism of amino acids, carbohydrates, lipids and nucleotides. A decline in pyruvic acid, and an increase in citric acid and fumaric acid in the gills and mantle of C. farreri indicated an alteration in energy metabolism, which may be attributed to increased ATP production in order to overcome the heat stress. Among the SDMs, 33 metabolites, including pyruvic acid, glycine and citric acid, were selected as potential biomarkers for heat stress response in C. farreri. In addition, a decline in glutamine and β-Alanine levels indicated oxidative stress in C. farreri exposed to heat, as well as an increase in the total antioxidant capacity (T-AOC). Our findings suggested C. farreri have the potential to adapt to heat stress by regulating energy metabolism and antioxidant capacity.

Assessment of impacts of climate change on rice and wheat in the Indo-Gangetic plains

In this paper, the climate change scenarios of A2 and B2 for 2070-2100 time scale (denoted as 2080) for several key locations of India and its impact on rice and wheat crops based on regional climate model (PRECIS) were described. The PRECIS projects an increase in temperature over most parts of India especially in the IGP (Indo-Gangetic Plains), the region that presently experiences relatively low temperatures. Extreme high temperature episodes and rainfall intensity days are projected to become more frequent and the monsoon rainfall is also projected to increase. Rabi (mid Nov-March) season is likely to experience higher increase in temperature which could impact and hence become threat to the crops which really require low temperature for their growth. Climatic variability is also projected to increase in both A2 and B2 scenarios. All these projected changes are likely to reduce the wheat and rice yields in Indo-Gangetic plains of India. It is likely that there will be more number of years with low yields occurs towards the end of the century. Such yield reductions in rice and wheat crops due to climate change are mediated through reduction in crop duration, grain number and grain filling duration. The yield loss will be more in A2 scenario compared to B2. These quantitative estimates still have uncertainties associated with them, largely due to uncertainties in climate change projections, future technology growth, availability of inputs such as water for irrigation, changes in crop management and genotype. These projections nevertheless provide a direction of likely change in crop productivity in future climate change scenarios.

Thermochronologic perspectives on the deep-time evolution of the deep biosphere

The Earth's deep biosphere hosts some of its most ancient chemolithotrophic lineages. The history of habitation in this environment is thus of interest for understanding the origin and evolution of life. The oldest rocks on Earth, formed about 4 billion years ago, are in continental cratons that have experienced complex histories due to burial and exhumation. Isolated fracture-hosted fluids in these cratons may have residence times older than a billion years, but understanding the history of their microbial communities requires assessing the evolution of habitable conditions. Here, we present a thermochronological perspective on the habitability of Precambrian cratons through time. We show that rocks now in the upper few kilometers of cratons have been uninhabitable (>∼122 °C) for most of their lifetime or have experienced high-temperature episodes, such that the longest record of habitability does not stretch much beyond a billion years. In several cratons, habitable conditions date back only 50 to 300 million years, in agreement with dated biosignatures. The thermochronologic approach outlined here provides context for prospecting and interpreting the little-explored geologic record of the deep biosphere of Earth's cratons, when and where microbial communities may have thrived, and candidate areas for the oldest records of chemolithotrophic microbes.

Drought and High Temperature Stress in Sorghum: Physiological, Genetic, and Molecular Insights and Breeding Approaches.

Sorghum is one of the staple crops for millions of people in Sub-Saharan Africa (SSA) and South Asia (SA). The future climate in these sorghum production regions is likely to have unexpected short or long episodes of drought and/or high temperature (HT), which can cause significant yield losses. Therefore, to achieve food and nutritional security, drought and HT stress tolerance ability in sorghum must be genetically improved. Drought tolerance mechanism, stay green, and grain yield under stress has been widely studied. However, novel traits associated with drought (restricted transpiration and root architecture) need to be explored and utilized in breeding. In sorghum, knowledge on the traits associated with HT tolerance is limited. Heat shock transcription factors, dehydrins, and genes associated with hormones such as auxin, ethylene, and abscisic acid and compatible solutes are involved in drought stress modulation. In contrast, our understanding of HT tolerance at the omic level is limited and needs attention. Breeding programs have exploited limited traits with narrow genetic and genomic resources to develop drought or heat tolerant lines. Reproductive stages of sorghum are relatively more sensitive to stress compared to vegetative stages. Therefore, breeding should incorporate appropriate pre-flowering and post-flowering tolerance in a broad genetic base population and in heterotic hybrid breeding pipelines. Currently, more than 240 QTLs are reported for drought tolerance-associated traits in sorghum prospecting discovery of trait markers. Identifying traits and better understanding of physiological and genetic mechanisms and quantification of genetic variability for these traits may enhance HT tolerance. Drought and HT tolerance can be improved by better understanding mechanisms associated with tolerance and screening large germplasm collections to identify tolerant lines and incorporation of those traits into elite breeding lines. Systems approaches help in identifying the best donors of tolerance to be incorporated in the SSA and SA sorghum breeding programs. Integrated breeding with use of high-throughput precision phenomics and genomics can deliver a range of drought and HT tolerant genotypes that can improve yield and resilience of sorghum under drought and HT stresses.

Single and Combined Salinity and Heat Stresses Impact Yield and Dead Pericarp Priming Activity.

In the face of climate change and the predicted increase in the frequency and severity of abiotic stresses (e.g., hot spell, salinity), we sought to investigate the effect of salinity (S), short episodes of high temperature (HS) and combination of salinity and high temperature (SHS), at the reproductive phase, on yield with a special focus on the properties of dead pericarps of Brassica juncea. Three interval exposures to HS resulted in massive seed abortion, and seeds from salt-treated plants germinated poorly. Germination rate and final germination of B. juncea seeds were slightly reduced in the presence of salt and SHS pericarp extracts. All pericarp extracts completely inhibited seed germination of tomato and Arabidopsis, but removal of pericarp extracts almost fully restored seed germination. Heat and salinity profoundly affected the accumulation of phytohormones in dead pericarps. Combined stresses highly reduced IAA and ABA levels compared with salt, and enhanced the accumulation of GA1, but abolished the positive effect of salt on the accumulation of GA4, JA and SA. Interestingly, pericarp extracts displayed priming activity and significantly affected seedling performance in a manner dependent on the species and on the origin of the pericarp. While control pericarps improved and reduced the seedlings’ performance of autologous and heterologous species, respectively, pericarps from salt-treated plants were harmless or improved heterologous seedling performance. Thus, the strategy employed by the germinating seed for securing resources is set up, at least partly, by the mother plant in conjunction with the maternal environment whose components are stored in the dead maternal organs enclosing the embryo.

How and why plant ionomes vary across North American grasslands and its implications for herbivore abundance.

Plant elemental content can vary up to 1,000-fold across grasslands, with implications for the herbivores the plants feed. We contrast the regulation, in grasses and forbs, of 12 elements essential to plants and animals (henceforth plant-essential), 7 essential to animals but not plants (animal-essential) and 6 with no known metabolic function (nonessential). Four hypotheses accounted for up to two thirds of the variation in grass and forb ionomes across 54 North American grasslands. Consistent with the supply-side hypothesis, the plant-essential ionome of both forbs and grasses tracked soil availability. Grass ionomes were more likely to harvest even nonessential elements like Cd and Sr. Consistent with the grazing hypothesis, cattle-grazed grasslands also accumulated a handful of metals like Cu and Cr. Consistent with the NP-catalysis hypothesis, increases in the macronutrients N and P in grasses were associated with higher densities of cofactors like Zn and Cu. The plant-essential elements of forbs, in contrast, consistently varied as per the nutrient-dilution hypothesis-there was a decrease in elemental parts per million with increasing local carbohydrate production. Combined, these data fit a working hypothesis that grasses maintain lower elemental densities and survive on nutrient-poor patches by opportunistically harvesting soil nutrients. In contrast, nutrient-rich forbs use episodes of high precipitation and temperature to build new carbohydrate biomass, raising leaves higher to compete for light, but diluting the nutrient content in every bite of tissue. Herbivores of forbs may thus be particularly prone to increases in pCO2 via nutrient dilution.

Increasing frequency of high-temperature episodes in potato growing regions of Western Australia and its impacts on plant and tuber growth

ABSTRACT Little is known about actual temperature regimes experienced by potato during the critical period of tuber bulking and their impact on plant and tuber growth outside the cool temperate production regions. We analysed the temperature data of potato growing regions in Western Australia (WA) and six other non-traditional potato growing regions for the number of days with maximum temperatures above 25, 30 or 35°C and their occurrence during tuber bulking. As anticipated, days with 25°C increased between 1985 and 2014 in all locations in WA. Surprisingly, across the other six locations, only Beersheba (Israel) showed a significant trend of increasing temperatures. Then, in a greenhouse experiment, we investigated how 1, 3, 6 and 9 days of 30°C applied shortly after tuber initiation influenced plant and tuber growth of potato cv. Royal Blue. Tuber mass and leaf area decreased linearly with increased duration of 30°C. In conclusion, potato grown in WA and other locations already experience growth limiting temperatures during tuber bulking and the frequency of hot days is increasing. Considering climate change and the expansion of potato production into non-traditional areas, more detailed examination of temperature regimes in other warm growing regions could be important for industry planning.

Differential Response to Single and Combined Salt and Heat Stresses: Impact on Accumulation of Proteins and Metabolites in Dead Pericarps of Brassica juncea.

Dead organs enclosing embryos, such as seed coats and pericarps, are emerging as important maternally-derived components of the dispersal unit that affect seed performance and fate. In the face of climate change and increased incidents of heatwaves, we sought to investigate the effect of salinity (S), short episodes of high temperature (HS), and combination of S + HS (SHS), at the reproductive phase, on the properties of dead pericarps of Brassica juncea. Proteome and metabolome analyses revealed multiple proteins and metabolites stored in dead pericarps whose levels and composition were altered under single and combined stress conditions. The protein profile of SHS showed a higher correlation with salt than with HS indicating the dominant effect of salt over heat stress. On the other hand, the analysis of metabolites showed that the profile of SHS has better correlation with HS than with salt. The integration of metabolic and proteomic data showed that changes in TCA cycle intermediates and certain amino acids (e.g., proline) under salt treatments (S and SHS) are highly correlated with changes in proteins involved in their biosynthetic pathways. Thus, accumulation of proteins and metabolites in dead pericarps is differently affected by single and combination of salt and heat stresses. Salinity appears to dominate plant response to combined stresses at the protein level, while heat appears to be the major factor affecting metabolite accumulation in dead pericarps.

Combined Effect of Hot Weather and Outdoor Air Pollution on Respiratory Health: Literature Review

Association between short-term exposure to ambient air pollution and respiratory health is well documented. At the same time, it is widely known that extreme weather events intrinsically exacerbate air pollution impact. Particularly, hot weather and extreme temperatures during heat waves (HW) significantly affect human health, increasing risks of respiratory mortality and morbidity. Concurrently, a synergistic effect of air pollution and high temperatures can be combined with weather–air pollution interaction during wildfires. The purpose of the current review is to summarize literature on interplay of hot weather, air pollution, and respiratory health consequences worldwide, with the ultimate goal of identifying the most dangerous pollution agents and vulnerable population groups. A literature search was conducted using electronic databases Web of Science, Pubmed, Science Direct, and Scopus, focusing only on peer-reviewed journal articles published in English from 2000 to 2021. The main findings demonstrate that the increased level of PM10 and O3 results in significantly higher rates of respiratory and cardiopulmonary mortality. Increments in PM2.5 and PM10, O3, CO, and NO2 concentrations during high temperature episodes are dramatically associated with higher admissions to hospital in patients with chronic obstructive pulmonary disease, daily hospital emergency transports for asthma, acute and chronic bronchitis, and premature mortality caused by respiratory disease. Excessive respiratory health risk is more pronounced in elderly cohorts and small children. Both heat waves and outdoor air pollution are synergistically linked and are expected to be more serious in the future due to greater climate instability, being a crucial threat to global public health that requires the responsible involvement of researchers at all levels. Sustainable urban planning and smart city design could significantly reduce both urban heat islands effect and air pollution.

Sorghum mitigates climate variability and change on crop yield and quality.

Global food insecurity concerns due to climate change, emphasizes the need to focus on the sensitivity of sorghum to climate change and potential crop improvement strategies available, which is discussed in the current review to promote climate-smart agriculture. Climate change effects immensely disturb the global agricultural systems by reducing crop production. Changes in extreme weather and climate events such as high-temperature episodes and extreme rainfalls events, droughts, flooding adversely affect the production of staple food crops, posing threat to ecosystem resilience. The resulting crop losses lead to food insecurity and poverty and question the sustainable livelihoods of small farmer communities, particularly in developing countries. In view of this, it is essential to focus and adapt climate-resilient food crops which need lower inputs and produce sustainable yields through various biotic and abiotic stress-tolerant traits. Sorghum, "the camel of cereals", is one such climate-resilient food crop that is less sensitive to climate change vulnerabilities and also an important staple food in many parts of Asia and Africa. It is a rainfed crop and provides many essential nutrients. Understanding sorghum's sensitivity to climate change provides scope for improvement of the crop both in terms of quantity and quality and alleviates food and feed security in future climate change scenarios. Thus, the current review focused on understanding the sensitivity of sorghum crop to various stress events due to climate change and throws light on different crop improvement strategies available to pave the way for climate-smart agriculture.