Event Abstract Back to Event Long-term results of peripheral nerve repair: A comparison of nerve anastomosis with ethyl-cyanoacrylate and epineural sutures Thomas Landegren1* 1 Karolinska Institutet, Department of Clinical Science and Education, Sweden There is a need for complementary surgical techniques which offers rapid and reliable primary repair of transected nerves. Anastomosis of a nerve with synthetic adhesive following a lesion has previously been shown to indicate recovery to an extent comparable to that of conventional techniques. The aim of this study was to quantify the morphological and functional recovery and evaluate the selectivity of muscle reinnervation after transection and repair of rat sciatic nerve, and compare epineural sutures with a synthetic ethyl-cyanoacrylate tissue adhesive. Six month after repair, when reinnervation had well been completed the tibial branch to the lateral gastrocnemius muscle and the caudal sural cutaneous nerve were examined with electrophysiological measurements of motor and sensory conduction velocity, motor nerve action potentials, and quantitative histological examinations. Furthermore, cholera toxin B technique of retrograde axonal tracing was used to evaluate the morphology, the number and the three-dimensional location of a-motoneurons in L5 spinal cord, innervating the lateral gastrocnemius muscle and the results were put in relation to the recorded wet weight of the muscle. There was functional reinnervation of motor and sensory nerves in both groups, as shown by equivalent recovery of motor and sensory conduction velocities, and motor nerve action potentials. Histological examination showed no significant difference in the mean diameter, fibre density or the number of regenerated myelinated motor and sensory axons distal to the repair site between the two groups. Moreover, independent of repair method, the redistribution of the motoneuron pool were markedly disorganized, had increased apparently in number, and were scattered throughout a larger volume of the spinal cord grey matter with a decrease in the synaptic coverage compared to controls. A reduction in muscle weight was observed as well. Differences compared to controls were statistically significant. We conclude that anastomosis of the nerve with ethyl-cyanoacrylate adhesive supports morphological and functional recovery comparable to that of conventional epineural sutures after a unilateral lesion of the sciatic nerve in adult rats. Keywords: cyanoacrylate, motoneuron, Nerve repair, peripheral nerve, Regeneration, retrograde tracing, spinal misdirection, synthetic adhesive Conference: Karolinska Institutet 200 years anniversary Symposium on Traumatic Injuries in the Nervous System, Stockholm, Sweden, 15 Sep - 16 Sep, 2010. Presentation Type: Presentation Topic: Traumatic Injuries in the Nervous System Citation: Landegren T (2010). Long-term results of peripheral nerve repair: A comparison of nerve anastomosis with ethyl-cyanoacrylate and epineural sutures. Front. Neurol. Conference Abstract: Karolinska Institutet 200 years anniversary Symposium on Traumatic Injuries in the Nervous System. doi: 10.3389/conf.fneur.2010.56.00011 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 07 Sep 2010; Published Online: 21 Sep 2010. * Correspondence: Dr. Thomas Landegren, Karolinska Institutet, Department of Clinical Science and Education, Stockholm, Sweden, Thomas.landegren@ki.se Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Thomas Landegren Google Thomas Landegren Google Scholar Thomas Landegren PubMed Thomas Landegren Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.