Liver Enzyme Activities Research Articles

Effect of Vitamins A, E, D3 Supplemented with Omega3 and Vitamins A, E, D3 (without Omega -3) on Biochemical Blood Parameters of Females Awassi Lamb

This study was conducted in the animal field of the University of Anbar College of Agriculture from 15/December/2021 to 15/February/2022. Twelve Iraqi Awassi female lambs were used in the experiment, at ranged of ages 2-3 months with average body weight 16.737 kg. The experimental animals were randomly divided into three groups, each group consisted of four lambs. The first group (G1) was considered the control group and left without any treatment, while the second group (G2) was treated with omega-3 supplemented with vitamins A, D3 and E of 1.5 CC, whilst the third group (G3) was treated with vitamins A, D3 and E of 1.5 CC by intramuscular injection, the lambs were injected with 6 times, the interval between each injection was 15 days. The lambs were nourished on the same concentrated diet, alfalfa hay, minerals bloke and water given adliptum. The result showed that G2 had significant increased (P≤0.05) on glucose concentration level as comported with G1. G3 had significant increase (P≤0.05) in urea concentration level as comported with G1 and G2. G3 had significant increased (P≤0.05) in Cholesterol and Triglyceride concentration level as comported with G1and G2. G1 had significant increase in LDL concentration level as comported with G2 and G3. T1 had significant increase in AST in G1, G2 and G3 as comported with T2 and T3. In conclusion, that treat a lamb with vitamins A, E, D3 supplemented with omega3 and vitamins A, E, D3 (without omega -3) led to improve lipid profile, liver enzyme activity and public health of lambs.

Evaluation of biochemical parameters in operating room staff exposed to radiation and anesthetic gases.

Exposure to ionizing radiations and other hazardous agents such as anesthetic gases pose serious risks to the health of healthcare workers. This study aimed to evaluate the changes in blood and biochemical parameters of the operating room staff exposed to ionizing radiations and remnants of anesthetic gas. This cross-sectional study was performed at (Ayatollah Taleghani Hospital). The control group was selected from different parts of the hospital that were not exposed to ionizing radiations and anesthetics, including the office, services, and treatment. The case group included all operating room personnel. Hematopoietic parameters such as complete blood count and WBC differential, and parameters of liver function such as serum activity of liver enzymes (ALT, AST, ALP, LDH) and serum bilirubin levels, fasting blood sugar, serum lipid profile, level of vitamin D and magnesium were measured for the exposed and referent subjects. Additionally, a checklist was used to gather data regarding the occupational variables and medical histories of the studied subjects. The mean values of Hb, Hct, Vitamin D, and MCHC, as well as the RBC count, were significantly lower in the exposed individuals than in the referent subjects. In contrast, the proportion of smokers was significantly higher in the exposed group than in the referent group. No significant differences were noted between exposed and unexposed groups as far as other parameters were concerned. However, no significant differences were noted between the case and control groups as far as other measured parameters were concerned. Likewise, no significant differences were noted between exposed and referent groups as far as blood types, history of underlying diseases, work history, working hours per month, number of morning and evening shift hours, type of diet, consumption of a high-fat diet a day before blood sampling, X-ray in the recent year, history of radiotherapy, and therapeutic agents use was concerned. Exposure of operating room staff to ionizing radiations and waste anesthetics gases is associated with subtle, subclinical prepathologic decreases in some hematopoietic parameters such as hemoglobin, hematocrit and MCHC levels, RBC count as well as vitamin D levels.

Preliminary Study to Assess the Impact of Dietary Rutin on Growth, Antioxidant Capacity, and Intestinal Health of Yellow Catfish, Pelteobagrus fulvidraco

This research aimed to examine the effects of dietary rutin supplementation on growth, body composition, serum biochemical indexes, liver enzyme activities and antioxidant-related genes expression, intestinal morphology, and microbiota composition of juvenile yellow catfish (Pelteobagrus fulvidraco). Rutin was added to the basal diets at doses of 0 (control), 100 mg/kg, and 500 mg/kg. Each diet was fed randomly into three tanks, each tank containing 30 fish with an initial body mass of (10.27 ± 0.62) g. The feeding trial was conducted in an indoor recirculating aquiculture system at 28 °C for 56 days. According to the findings, the inclusion of 100 mg/kg rutin significantly improved the growth performance of yellow catfish and reduced the feed conversion ratio; however, the growth promotion effect was diminished when the diet was supplemented with 500 mg/kg of rutin. The inclusion of 500 mg/kg rutin in the diet significantly reduced the level of crude lipid and protein of the whole fish. Serum activities of alkaline phosphatase, albumin, and total protein were all significantly increased when fish were fed the diet supplemented with 500 mg/kg rutin, while serum glucose was significantly lower compared to the control group. Meanwhile, dietary rutin at a concentration of 500 mg/kg significantly induced the hepatic mRNA expressions of antioxidant-related genes (including Cu/Zn-SOD, Mn-SOD, CAT, GPx) and inflammatory-associated genes (including TNFα, IL-10, LYZ). Incorporating rutin at doses of 100 mg/kg and 500 mg/kg into the diets resulted in a notable increase in superoxide dismutase (SOD) activity, while simultaneously reducing malondiadehyde (MDA) content in the liver and intestine. Intestinal villus height, villus width, muscular thickness, and lumen diameter were significantly increased with the administration of 500 mg/kg of dietary rutin. Gut microbial diversity analysis indicated that supplementing diets with 100 mg/kg and 500 mg/kg rutin significantly enhanced the abundance of Cetobacterium while decreasing Plesiomonas richness. In conclusion, dietary rutin levels at 100 mg/kg could enhance the growth, antioxidant capability, and intestinal health of yellow catfish under present experimental conditions.

The efficacy and safety of cannabidiol as adjunct treatment for drug-resistant idiopathic epilepsy in 51 dogs: A double-blinded crossover study.

Approximately 30% of dogs with idiopathic epilepsy (IE) are drug-resistant. Recent studies have suggested cannabidiol (CBD) may be an effective anticonvulsant in dogs with IE. To evaluate the addition of CBD to antiseizure drugs (ASDs) on seizure frequency and to report adverse events in dogs with drug-resistant IE. Fifty-one dogs. Dogs having at least 2 seizures per month while receiving at least 1 ASD were included in the trial. Double-blinded placebo-controlled crossover study. The 5 mg/kg/day dosage met futility requirements after 12 dogs, and a dosage of 9 mg/kg/day was used in the next 39 dogs. Dogs were randomly assigned to receive CBD or placebo for 3 months, with a 1-month washout period between oils. Total numbers of seizures and seizure days were recorded. Diagnostic testing was performed periodically throughout the trial. At the 9 mg/kg/day dose, the decrease in total seizure frequency was significant compared with placebo. A 24.1% decrease in seizure days occurred in dogs receiving CBD and a 5.8% increase occurred in dogs receiving placebo (P ≤ .05). No significant difference was found in the number of responders (≥50% decrease in total seizures or seizure days). Liver enzyme activities increased at both dosages. Decreased appetite and vomiting were more common in the CBD phase (P ≤ .05). Cannabidiol decreased total seizures and seizure dayscompared to placebo when administered to dogs PO at 9 mg/kg/day. Liver enzymes should be monitored with administration of CBD in dogs.

The effect of Moringa oleifera crude extract on liver cell line, HepG2

BackgroundThe liver plays a crucial role in the body's metabolic and detoxification processes. Given its importance, compromised liver function can negatively impact the body’s metabolic and physiological function. Liver diseases can result from several factors, including exposure to toxins, alcohol consumption, and viral infections. Therefore, finding natural remedies for liver protection and treatment is important. Moringa oleifera is a tree known for its various medicinal properties, including hepatoprotective effects. This study aimed to investigate the potential of M. oleifera seed extract in protecting liver cells.MethodsIn this study, dried-seed powder of M. oleifera was extracted using extraction solvents, methanol, and ethanol. HepG2 cells were cultured and treated with different concentrations of the extracts. The antioxidative activity, cell viability, and antiproliferation were assessed using the total antioxidant capacity assay (TAC) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Additionally, liver enzyme activity was determined through alkaline phosphatase and aspartate aminotransferase activity assays.ResultsThe extracts had varying effects on liver cells depending on the concentration and time of exposure. Lower concentrations (50 mg/l and 100 mg/l) have mild stimulatory effects/minimal impact on metabolic activity, while higher concentrations (200 mg/l and 400 mg/l) tend to decrease metabolic activity, especially at later time points. Moreover, the extracts effectively reduced the levels of the liver enzyme AST, indicating their ability to mitigate liver injury.ConclusionThe study concludes that the crude extracts of M. oleifera seeds exhibit potential as a natural remedy for liver diseases. The effects of M. oleifera extract suggest that it has potential as a preventive and therapeutic agent for liver damage. This study highlights the importance of exploring natural remedies for liver protection and treatment.

Correlation of Plasma Cell Percentage and Monoclonal Gammapathies with Biochemical Parameters in Multiple Myeloma Patients: A Retrospective Study

Background: Multiple myeloma is characterized by the uncontrolled proliferation of monoclonal plasma cells in the bone marrow, leading to the production of non-functional intact immunoglobulin chains. This retrospective study aimed to investigate the correlation between plasma cell percentage (below 60% and above 60%) in different types of monoclonal gammapathies (IgA, IgG) and various biochemical parameters.Methods: This analysis was conducted at the Division of Biochemistry in the Malabar Cancer Centre, a tertiary care cancer center in Kerala, India. A total of 89 patient case reports were reviewed, and complete treatment data were available for 60 patients. The study aimed to evaluate the relationship between plasma cell percentage (below 60% and above 60%) in different types of monoclonal gammapathies (IgA, IgG) and various biochemical parameters. Results: The majority of the patients enrolled in the study were above 60 years of age, and more men were included than women. Among the parameters studied, a significant increase in plasma protein level was observed in the group with a plasma cell percentage below 60% and IgG type monoclonal gammapathy (P value = 0.014, p < 0.05). Additionally, there was a significant elevation in WBC count in patients with plasma cell percentage below 60% and IgG type MM when compared to IgA type MM patients (p = 0.023, p < 0.05). However, no significant changes were observed in plasma protein level, liver enzyme activity, renal function, serum electrolytes, CBC, and calcium level between patients with plasma cell percentages above and below 60% in multiple myeloma. Conclusion: This study found that only a few biochemical and hematological parameters showed significant deviations between plasma cell percentages above and below 60% in different types of monoclonal gammapathies in multiple myeloma patients.

Serum Bile Acids Concentrations and Liver Enzyme Activities after Low-Dose Trilostane in Dogs with Hyperadrenocorticism.

Hyperadrenocorticism (HAC) often leads to vacuolar hepatopathy. The impact of trilostane treatment on serum total bile acids (SBAs) concentrations in dogs with HAC remains unknown. This study investigated SBAs concentrations in healthy dogs and those with HAC following trilostane therapy. Ten healthy dogs and fifteen dogs with HAC were prospectively enrolled. A biochemistry profile and pre- and post-prandial SBAs concentrations were determined in each dog. Dogs with HAC were reassessed at 1 and 3 months after the initiation of trilostane treatment. Dogs with HAC had significantly higher serum ALT, ALP, and GGT activities, and cholesterol, triglyceride, and pre-prandial SBAs concentrations compared to healthy dogs. After 3 months of trilostane treatment, polyuria/polydipsia and polyphagia were completely resolved in 42.8% and 35.7%, respectively. Significant improvements in serum ALT and ALP activities and cholesterol concentrations were observed within 1-3 months of trilostane treatment. However, pre- and post-prandial SBAs concentrations did not significantly decrease. These findings suggest that treatment with low-dose trilostane for 3 months appears to reduce serum liver enzyme activities, but not SBAs concentrations. Further investigation is warranted to explore the effects of low-dose trilostane treatment on SBAs concentrations for a longer duration or after achieving appropriate post-ACTH cortisol levels.

Jatonik polyherbal mixture induced rat liver MMPT pore opening in normal Wistar rat: In vitro and in vivo studies

ObjectiveTo assess acute toxicity, the in vitro and in vivo effects of methanol and ethyl acetate extracts (JME and JEE) of Jatonik polyherbal mixture on some mitochondria-related parameters and their effect on the activity of some liver enzymes. MethodsAcute toxicity of JME and JEE was determined using Lorke’s method. In vitro and in vivo opening of the mitochondrial membrane permeability transition pore (MMPT pore) was spectrophotometrically assayed. Production of malondialdehyde (MDA) as an index of lipid peroxidation and the activity of mitochondrial ATPase was evaluated in vitro and in vivo and the effect of JME and JEE on the activity of liver enzymes such as alkaline phosphatase (ALP), aspartate and alanine aminotransferase (AST and ALT) and gamma-glutamyl transferase (GGT) was also investigated. ResultsJME had an LD50 of 3 808 mg/kg b.w whereas JEE had an LD50 greater than 5 000 mg/kg b.w. of rats. After the rats have been fed with both extracts, a photomicrograph of a piece of liver tissue showed no apparent symptoms of toxicity. From the in vitro and in vivo studies, both extracts prompted intact mitochondria to open their MMPT pores. When compared to the control, lipid peroxide product release and ATPase activity were significantly increased (P < 0.05) in vitro and in vivo. The activities of AST, ALT, and GGT were all reduced at 50 mg/kg when treated with JME, but the activity of AST was considerably enhanced when treated with JEE (P < 0.05). The results revealed that both JME and JEE of the Jatonik polyherbal mixture had low toxicity, profound MMPTpore induction, and enhanced ATPase activity, but an increased MDA production. ConclusionJatonik extracts may be a promising target for drug development in diseases where there is dysregulation of apoptosis, however, further studies are needed to better clarify the molecular mechanism involved in these phenomena.

Modulation of Hepatic Functions by Chicory (Cichorium intybus L.) Extract: Preclinical Study in Rats.

The liver is important in detoxifying organisms from xenobiotics, supporting immune functions, and metabolizing lipids and glucose. In addition, a growing number of drug-induced liver injuries and diseases associated with liver dysfunction make the development of phytodrugs targeting multiple liver functions particularly crucial. Therefore, we investigated the effects of a novel chicory extract prepared from aerial parts of the wild Cichorium intybus L. plant (CE) on liver enzymes and on lipid and glucose metabolism in rats with acute liver injury or hyperlipidemia. A single subcutaneous injection of mercury chloride induced an acute liver injury. Hyperlipidemia was induced by a single intraperitoneal injection of Tween-80 or by feeding rats with cholesterol and mercazolil for 28 days. Under varying regimens, the experimental rats received 100 mg/kg b.w. or 500 mg/kg b.w. of CE. CE treatment ameliorated acute liver injury by reducing liver enzyme activity, bilirubin, glucose, and lipid levels. Treatment of hyperlipidemic rats with CE effectively reduced serum lipid and glucose levels. The data obtained in this study suggest that chicory-based phytodrugs may be used to effectively treat acute liver injury and for the prophylaxis or treatment of diseases such as hyperlipidemia, type 2 diabetes, and metabolic syndrome. Clinical trials are needed to prove the effectiveness of chicory extract in human patients.

Correlative Study on the Hepatorenal and Hormonal Alterations in Perimenopausal Women in Enugu, South East of Nigeria

Background: The term perimenopause refers to a woman's shift from a reproductive to a nonreproductive stage. Many hormonal and biochemical changes accompany this transformation. There is insufficient information in the association between hormonal changes, Liver enzymes activities, and renal functions in perimenopause. This study evaluated the hormonal and hepatorenal alterations in perimenopausal and premenopausal women in Enugu State, Nigeria. Methods: A total of 180 apparently healthy women were recruited for this cross-sectional study. 90 perimenopausal women (mean age = 49 years) and 90 premenopausal women (mean age = 29 years) served as test and control subjects. Subjects' anthropometric indices (blood pressure, waist circumference (WC), height, and weight) were measured. five milliliters of fasting blood was collected from the subjects to test for Estradiol (E2), Luteinizing hormone (LH), Follicle stimulating hormone (FSH), Alanine transaminase (ALT), Aspartate transaminase (AST), Alkaline phosphatase (ALP), Protein, Urea, Creatinine, and Fasting blood glucose (FBG) using standard biochemical procedures. Results: Blood pressure, FBG, WC, FSH, LH, ALT, AST, Creatinine levels were substantially higher and Estradiol was lower in perimenopausal women when compared to premenopausal women (P&lt;0.05). There exist significant positive correlations among perimenopausal women in FSH and LH with AST and ALT, Estradiol with protein, Urea vs Creatinine, while Estradiol was negatively associated with ALT, AST, ALP and FSH. Conclusion: Perimenopausal women are likely prone to a higher risk of liver and renal dysfunction. Therefore, the need for regular evaluation of liver and kidney parameters during perimenopause is encouraged.

Differential diagnosis of unconjugated hyperbilirubinemiadetected after coronary artery stenting

Introduction. An increase in bilirubin and liver enzyme activity may be one of the side effects of statin therapy, often occurring in patients after AMI and coronary artery stenting, or in high and very high risk individuals on high and moderate intensity statin therapy. The frequency of occurrence of increased transaminases and bilirubin is according to different authors. Therefore, in terms of differential diagnosis, the cardiologist should consider Gilbert’s syndrome as a possible cause of hyperbilirubinemia. Description of the clinical case. The article considers a clinical case of differential diagnosis of non-conjugated hyperbilirubinemia detected in a patient after coronary artery stenting. The level of hemoglobin, erythrocytes, reticulocytes did not differ from normal values and did not change over time. This made it possible to exclude the hemolytic genesis of hyperbilirubinemia. Genetic testing was used to establish the homozygous form of Gilbert’s syndrome. However, the presence of fibrotic changes in the liver, an increase in not only unconjugated, but also conjugated bilirubin, hypertriglyceridemia, dyslipidemia, and stenosing atherosclerosis of the coronary arteries did not allow us to state that the patient had only Gilbert’s syndrome. Discussion. According to recent studies, this disease is characterized by a benign course and reduces the risk of developing cardiovascular diseases due to the antioxidant effect of bilirubin. In addition to Gilbert’s syndrome, the patient was diagnosed with an erased form of non-alcoholic fatty liver disease associated with metabolic syndrome. Conclusion. The disease was caused by insulin resistance, a high-calorie diet, excess consumption of saturated fats, refined carbohydrates, and a sedentary lifestyle. The drugs of choice in this case are statins, ezetemibe, and ursodeoxycholic acid. Their appointment allows not only to reduce cardiovascular risk, but also to slow down the further progression of liver fibrosis.

A Study of Glucose-6-Phosphate Dehydrogenase Deficiency among Children attending the Emergency Hospital in Zakho City Kurdistan Region, Iraq

Background: G6PD deficiency, which affects 400 million individuals globally, is an X-linked hereditary enzymopathy that causes acute hemolysis after exposure to specific oxidative agents such as fava beans, more than 37 drugs, and infections viral or bacterial. Neonatal jaundice worsened by kernicterus is one of the condition's significant consequences. It can be prevented by avoiding the oxidative factors that cause a hemolytic episode alongside neonatal screening programs for early detection of afflicted individuals. Objectives: This research aimed at investigating the variability in clinical and biochemical manifestations among children with G6PDD. This is the first study to be carried out in the Zakho area. Methods: A cross-sectional study was used, recruiting 112 children attending Zakho Emergency Hospital from January 2022 to April 2023. Laboratory aids involved in obtaining CBC, liver enzyme activities, and blood grouping were all investigated. The IBM SPSS 26 program was used to analyze the obtained data. Results: There was no statistically significant difference between male and female patients in terms of age, time of admission, hemoglobin level, WBC count, and liver enzymes. The majority of cases who visited Zakho Emergency Hospital within 72 hours of exposure to the triggering agent were males (67.9%), with the most common clinical features being jaundice, dark urine, and abdominal pain. Conclusion: In this study, the three main symptoms in G6PD deficient patients with acute hemolysis were pallor, jaundice, and black urine. These signs appeared several hours or even days after consuming fava beans. The primary clinical symptoms and the patient's gender, family history, or prior newborn jaundice were not related, according to our research.

POSSIBILITIES OF USING THE ANALYSIS OF OXIDANT-ANTIOXIDANT METABOLIC PROCESSES AND ION EXCHANGE IN PREDICTING THE DEVELOPMENT OF POSTOPERATIVE COMPLICATIONS IN SMALL BOWEL SURGERY

Summary. Introduction. The work is devoted to the study of the possibilities of using the analysis of oxidant-antioxidant metabolic processes and ion exchange in predicting the development of postoperative complications during operations on the small intestine in conditions of a compromised abdominal cavity.&#x0D; The aim of the study. To determine the peculiarities of the flow of oxidant-antioxidant metabolic processes and ion exchange during operations on the small intestine in conditions of intra-abdominal infection and to study the clinical effectiveness of their assessment.&#x0D; Materials and methods. Research was conducted at the Institute of General and Emergency Surgery of the State University named after V.T. Zaitsev National Academy of Sciences of Ukraine”. The study properly included 88 patients who were divided into three groups: a control group (20 patients), a study group (50 patients).&#x0D; Results and discussion. The most common criteria for assessing the viability of an ischemic intestine are subjective assessments of color, motility, and pulsation of mesenteric vessels both during examination and after physical or pharmacological effects. However, such an assessment is subjective, does not always correspond to the actual condition of the intestinal wall, which leads either to an unjustified expansion of the scope of the intervention, or to an erroneous overestimation of the vital function of the damaged intestine.&#x0D; As a result of the research, a decrease in the phagocytic activity of neutrophils was found in patients who underwent resection of intestinal segments. In these patients, the development of endogenous intoxication was characterized by a decrease in the level of platelets and indicators of the blood anticoagulation system, leukopenia and methemoglobinemia, which requires antioxidant, antitoxic, as well as anticoagulant and immunological correction.&#x0D; The study of the exchange of metal ions in the blood serum of operated patients revealed dynamic changes in their content, iron ions (Fe2+); zinc (Zn2+); copper (Cu2+). The study of ion exchange of metals in the serum of the studied patients revealed significantly (p&lt;0.05) higher levels of iron and copper, which can have an important diagnostic and prognostic value when justifying the scope of surgical intervention.&#x0D; The results of studying the state of oxidant-antioxidant processes in the studied patients revealed an increase in the level of lipid peroxidation products: malondialdehyde (secondary products) and diene conjugates (end products); since the LPO system is a primary predictor of future immunodeficiency.&#x0D; The correlation analysis revealed a direct correlation between the initial content of cryoglobulins and the presence of postoperative complications (r=0.56, p=0.07 and r=0.53, p=0.052). Detection of high concentrations of cryoglobulins in the blood serum of operated patients already in the first hours allowed us to assume the presence of maximal autoimmune changes in intra-abdominal infection.&#x0D; The study of the spectrum of serum protein fractions, lipid peroxidation, the state of antioxidant protection, the activity of liver enzymes, factors of humoral and cellular immunity, revealed pronounced endotoxicosis, functional damage to hepatocytes, a violation of the immune status of the body, which correlated with the severity of pathological changes against the background of intra-abdominal infection.&#x0D; Conclusions. Analysis of oxidant-antioxidant metabolic processes, bioenergetic state testified to the stimulation of free radical processes, activation of lipid peroxidation, inhibition of antiradical protection in the studied patients, which in the complex stimulates the development of membrane pathology against the background of endogenous intoxication and inhibition of bioenergetics; there are processes of primary inhibition of non-specific resistance of the organism, in combination with a violation of the regeneration of individual links of the immune system.

Diagnosis and treatment of metabolic-associated fatty liver disease in patients with type 2 diabetes mellitus. Review

Metabolic‑associated fatty liver disease (MAFLD) is a new clinical definition of fatty liver disease that changes the definition of non‑alcoholic fatty liver disease (NAFLD) from a disease of exclusion to a disease of inclusion, in which the main pathogenic processes originate from metabolic dysfunction, primarily in type 2 diabetes mellitus (T2DM) and obesity. In recent years, MAFLD has become an epidemic and is closely associated with T2DM and a significant synergistic increase in the risk of cardiovascular disease. The presence of MAFLD significantly increases the risk of T2DM development, and the risk of new onset of T2DM in patients with MAFLD doubles. When managing patients with MAFLD and T2DM, the presence of fibrous nonalcoholic steatohepatitis (NASH) should be actively detected, for which non‑invasive indicators should first be used, such as FIB‑4 and FNI, which are available at the level of primary care. Despite evidence of utility in improving diagnosis, the effectiveness of many of the above non‑invasive tests for detecting fibrosis, particularly in unselected populations with advanced fibrosis, is low and generally remains uncertain at the primary care level. Modifications of lifestyle and diet have traditionally been considered the best therapeutic option for MAFLD in T2DM, with weight loss of ≥7% significantly improving steatosis, leading to reductions in NASH activity and glycemia. At the same time, only 40% of patients achieve this goal and reduce the activity of steatohepatitis, emphasizing the difficulty in treating MAFLD only by lifestyle changes. Nevertheless, mandatory dietary and lifestyle modification should still be recommended for patients with MAFLD, along with antidiabetic drugs that can reduce liver enzyme activity, inflammation, and fibrosis, such as PPARγ inhibitors, SGLT‑2i, GLP‑1 RA, as well as dual GIP and GLP‑1 RA agonists.&#x0D;

Improving productive performance, immunity, and health status of growing rabbits by using honey bee venom (Apis mellifera).

To investigate the effect of bee venom (BV) as a natural growth promotor on growing rabbits as an alternative to antibiotics, sixty 35-day-old Californian male rabbits with an average body weight of 584 ± 9 gm were randomly divided into five equal groups as follows: The 2nd group received drinking water supplied with 10 mg Oxytetracycline (OXT), while the 3rd, 4th, and 5th groups received 2, 4 and 8 mg bee venom (BV)/kg body weight/day in drinking water, and the first group was served as a control group. The growth performance features were positively impacted by adding BV (p ≤ 0.01) compared to the control, whereas LBW and BWG increased and FI reduced. Significantly improved carcass characteristics (p ≤ 0.01) as a result of the BV supplementation. Blood characteristics showed a significant reduction (p ≤ 0.01) in liver enzyme activities and Cholesterol, Triglycerides, and Low-density lipoproteins Cholesterol (LDL) as affected by BV treatment; inversely, total protein and globulin were significantly increased (p ≤ 0.01). Similarly, BV had a positive effect (p ≤ 0.01) on anti-oxidant status (Total anti-oxidant capacity (TAC), Glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT)). In contrast, the lipid peroxidation biomarker (Malondialdehyde (MDA)) was significantly decreased. The immunoglobulin (IgG and IgM) was significantly increased (p ≤ 0.01) by BV treatment. There was a positive effect of low BV levels on decreasing both cecum TBC and pathogenic bacterial count (Salmonella spp., E.coli spp., Proteus spp., and Clostridia spp.) that was significant (p ≤ 0.01). In conclusion, BV can be a natural growth promoter to enhance growth performance traits, immunological and anti-oxidative responses, and reduce pathogenic bacteria in the hindgut of growing rabbits.

Effects of Rutin and Selenomethionine in Selenium-Enriched Tartary Buckwheat Roasted Grain Tea on Glucose and Lipid Metabolism in db/db Mice

Rutin and selenomethionine in selenium-enriched Tartary buckwheat roasted grain tea have certain effects on glucose and lipid metabolism in type II diabetes mellitus, but the mechanism of intervention is still unclear. This study utilized selenium-enriched Tartary buckwheat tea, rutin, and selenomethionine as research materials, with db/db mice serving as the research subjects. Diabetes control (DC), positive control group (PC), rutin group (RC), selenomethionine group (SC), low dose selenium-enriched Tartary buckwheat tea group (TBG-L), medium dose group (TBG-M) and high dose group (TBG-H) were set up. The effects of selenium enriched Tartary buckwheat tea, rutin and selenomethionine on glucose and lipid metabolism in db/db mice were investigated by blood biochemical analysis, liver function and oxidative stress measurement, histopathology and glycogen determination and mRNA expression analysis. Selenium-enriched Tartary buckwheat tea and its active ingredients rutin and selenomethionine can reduce fasting blood glucose and glucose resistance in db/db mice. Dyslipidemia of db/db mice was improved to some extent. The changes of MDA, CAT, T-SOD and GSH-Px enzyme activities in liver also indicate that TBG can improve the oxidative stress of liver and exert liver protection activities. Selenium-enriched Tartary buckwheat tea, rutin and selenomethionine can mediate glycogen storage by increasing glycogen synthesis and inhibiting gluconeogenesis in the liver of diabetic db/db mice, and can effectively mediate the expression of related genes during gluconeogenesis. Among the selenium-enriched Tartary buckwheat groups, the effect of TBG-H group was the most obvious, which indirectly indicated that rutin and selenomethionine could play synergistic roles in gluconogenesis.

Impacts of dietary lipids derived from animal or vegetable sources on healthy rats

ABSTRACT This study sought to assess the health impact of feeding adult healthy rats with either 7% (w/w) animal-derived fat (subcutaneous rump fat or peri-kidney fat) from domestic buffalo or vegetable oil (corn oil or olive oil) for 8 weeks on bioactivity; lipid profile; hepatic and cardiac histological changes. Twenty-four male albino rats were randomly categorized into four dietary groups (six rats/group): 1) diet contains 7% (w/w) corn oil; 2) diet contains 7% (w/w) olive oil; 3) diet contains 7% (w/w) subcutaneous rump fats and 4) diet contains 7% (w/w) Peri-kidney fats. Animal fats intake significantly increased serum total lipids (TL), triacylglycerol (TG), low-density lipoprotein cholesterol (LDLC), cardiac troponin-I (CTn-I) concentration as well as alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH) activities compared to vegetable oils intake. In histopathological research, liver and heart sections excised from rats consumed animal-derived fats showed several signs of extensive damage, including inflammation as well as fibrosis. Compared to animal-derived fats, vegetable oils consumption markedly increased serum high-density lipoprotein cholesterol (HDLC) concentration and exhibited a potential attenuation effect on liver and heart enzymes activities and a potential role in the reduction of TG in the bloodstream of rats as well as revealed normal liver and heart histopathological architectures. In conclusion, this study suggests that, replacing animal fats, especially peri-kidney fat with vegetable oils, particularly olive oil in diets, liver and heart can be protected.

Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B.

Phthalates are widely distributed in our environment due to their usage in many industries, especially in plastic production, which has become an essential part of daily life. This investigation aimed to assess the potential remedial influence of lutein, a naturally occurring carotenoid, on phthalate-triggered damage to the liver and kidneys. When di-(2-ethylhexyl) phthalate (DEHP) was administered to male albino rats over sixty straight days at a dosage of 200 mg/kg body weight, it resulted in a significant increase in the serum activity of liver enzymes (AST, ALT, and GGT), alpha-fetoprotein, creatinine, and cystatin-C, as well as disruptions in the serum protein profile. In addition, intoxication with DEHP affected hepato-renal tissues' redox balance. It increased the content of some proinflammatory cytokines, nuclear factor kappa B (Nf-κB), and apoptotic marker (caspase-3); likewise, DEHP-induced toxicity and decreased the level of anti-apoptotic protein (Bcl-2) in these tissues. Lutein administration at a dose level of 40 mg/kg b.w efficiently facilitated the changes in serum biochemical constituents, hepato-renal oxidative disturbance, and inflammatory, apoptotic, and histopathological alterations induced by DEHP intoxication. In conclusion, it can be presumed that lutein is protective as a natural carotenoid against DEHP toxicity.

Effect of combined seeds of Sesemum indicum and Moringa oleifera compounded feed on liver function markers of alloxan-induced diabetic albino rats

This study investigated the effect of combined seeds of Sesemum indicum (SI) and Moringa oleifera (MO) on liver function markers in alloxan-induced diabetic albino rats. 54 albino rats were divided into 9 groups of 6 animals each. Group 1 served as the normal control and received standard feed diet, Group 2 (Diabetic control) received intraperitoneal injection of 140mg/kg of Alloxan, Group 3 (Positive control) received 5mg/kg of glibenclamide, Group 4; diabetic rats fed diet containing 15% of SI. Group 5; diabetic rat fed with 15% of MO diet, Group 6; diabetic rat fed with combination of 15% SI and 15% MO compounded diet. Group 7; diabetic rat fed with 30% of SI diet. Group 8; diabetic rat fed with 30% of MO diet. Group 9; diabetic rat fed with combination of 30% MO and 30% SI diet. The Feed was compounded and was given daily for 14 days. Serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total protein, albumin, and bilirubin were estimated to assess hepatic function using spectrophotometric method. Results showed a significant decrease in ALT, ALP, AST, and bilirubin levels when fed with compounded feeds at (p&lt;0.05). Also feeding of diet with combined seeds significantly (P&lt;0.05) increased the total protein and albumin in induced rats. Overall, compounded feed with combined seeds of SI and MO exhibited a promising anti-diabetic activity and normalized the liver enzyme activities which appeared comparable to the control glibenclamide in alloxan-induced diabetes hence, may be recommended for treatment/management of diabetes mellitus.

Evaluation of liver enzymes activity and some trace elements in sickle cell anemia patients in Basrah Governorate – Iraq.

Sickle cell anemia is one of genetic diseases caused by hemoglobin biochemical disorder in especially in polypeptidic chain of this protein. The study was conducted from October 2019 to December 2020 and included two sets of samples Patient and Healthy .The current study was curried out to evaluate some enzymes and trace elements in patients with sickle cell anemia. The levels of copper (Cu) were estimated spectrophotometrically by reaction of copper with 4 – (3,5 – dibromo – 2- pyridylazo) – N – ethyl sulphopropylaniline to produce a chelating complex.The determination was carried out at wavelength equal to 520 nm. Finally, absorbances were recorded and they are proportional to concentration of copper in the sample . Also, the levels of zinc in serum of blood belonging to sickle cell anemia (SCA) were investigated and estimated by reaction of zinc with chromogen existing in the characteristic kit to produce coloured complex. The absorbances were recorded spectrophotometrically which are proportional to concentration of zinc in blood sample . The levels of biochemical variables were estimated using ARCHITECT c4000 systems provided by ABBOTT Company, which are designed with advanced technology, accuracy in clinical results and ease of handling. The device uses a sample size at a rate of 6µL in all measurements of chemical variables.The total values of the current results were made and expressed by calculation of the mean ± standard deviation. Analysis of all results data were carried out by using a statistical procedure according to analysis of variance univariate . Statistical programmer of social science (SPSS ,v.23 ) was depended for calculation of significant differences for the different groups of patients infected by SCA disease and healthy persons . The correlation regression coefficient (r) was used and depended to distinguish between the means of groups belonging to all patients and healthy individuals ( control group ) .The p-value was agreed for less than 0.05 to the limit of lowest significance . A highly significant difference was recorded belong to levels of GPT, ALP and GOT in patients compared with healthy individuals (control group) according to age and sex factor.