Changes In Environmental Temperature Research Articles

Effects of drought-induced stress on nematode communities in aquatic and terrestrial habitats of the Nebraska Sandhills

IntroductionGlobal change events (e.g., worsening drought) are increasing environmental stress in a variety of terrestrial and aquatic habitats. The degree to which communities in soils and sediments are driven by temporal environmental changes across multiple habitat types from the same region is not clear.MethodsWe used nematodes, a common bioindicator of soil and sediment health, to determine how community diversity and composition are altered by rising alkalinity across lakes, shorelines, and prairies in the western Nebraska Sandhills. We sampled these three habitats from five lake basins spanning an alkalinity gradient (pH 7–11) across three years (2019, 2020, 2021). During our sampling, the Sandhills experienced a range of drought intensities, with 2019 being a wet year, followed by severe drought in 2020, and abnormally dry/moderate drought in 2021. To determine if diversity and composition of nematodes responded to increased alkalinity and drought-induced stress we used different modelling approaches, including Random Forest and pairwise comparisons.Results and discussionOverall, nematode diversity in lakes was most affected by increasing alkalinity over time, whereas in shorelines and prairies diversity was most reliant on bacterial diversity and potential nematode-nematode interactions. In comparison to shorelines and prairies, community composition in lakes was the least variable and consistently driven by pH and lake water levels. In contrast, compositions in the shorelines and prairies were more variable and explained at varying degrees by pH, year, lake basin, and climate-associated variables. In addition, relative abundance and compositional nature of select copious taxa were highly unpredictable, indicating potential instability in these habitats. Future research is necessary to address the ecologic stability of the Sandhills and determine where conservation efforts are most needed.

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Contrarily to chronic stresses, acute (i.e., fast and dramatic) changes in environmental factors like temperature, radiation, concentration of toxic substances, or pathogen attack often lead to DNA damage. Some of the stress factors are genotoxic, i.e., they damage the DNA via physical interactions or via interference with DNA replication/repair machinery. However, cytotoxic factors, i.e., those that do not directly damage the DNA, can lead to secondary genotoxic effects either via the induction of the production of reactive oxygen, carbon, or nitrogen species, or via the activation of programmed cell death and related endonucleases. The extent of this damage, as well as the ability of the cell to repair it, represent a significant part of plant stress responses. Information about DNA damage is important for physiological studies as it helps to understand the complex adaptive responses of plants and even to predict the outcome of the plant’s exposure to acute stress. Single cell gel electrophoresis (Comet assay) provides a convenient and relatively inexpensive tool to evaluate DNA strand breaks in the different organs of higher plants, as well as in unicellular algae. Comet assays are widely used in ecotoxicology and biomonitoring applications; however, they are still relatively rarely used in physiological studies. In this review, we provide an overview of the basic principles and of useful variations of the protocols of Comet assays, as well as of their use in plant studies, in order to encourage plant physiologists to include this tool in the analysis of plant stress responses.

Is thermal sensitivity affected by predation risk? A case study in tadpoles from ephemeral environments.

Changes in environmental temperature may induce variations in thermal tolerance and sensitivity in ectotherm organisms. These variations generate plastic responses that can be analyzed by examining their Thermal Performance Curves (TPCs). Additionally, some performance traits, like locomotion, could be affected by other factors such as biological interactions (e.g., predator-prey interaction). Here, we evaluate if the risk of predation modifies TPCs in Mendoza four-eyed frog (Pleurodema nebulosum, Burmeister, 1861) and Guayapa's four-eyed frog (Pleurodema guayapae, Barrio, 1964), two amphibian species that occur in ephemeral ponds in arid environments. We measured thermal tolerances and maximum swimming velocity at six different temperatures in tadpoles under three situations: control, exposure to predator chemical cues, and exposure to conspecific alarm cues. TPCs were fitted using General Additive Mixed Models. We found that curves of tadpoles at risk of predation differed from those of control mainly in thermal sensitivity parameters. Our work confirms the importance of biotic interactions have in thermal physiology.

Insulin at the intersection of thermoregulation and glucose homeostasis

Mammals are protected from changes in environmental temperature by altering energetic processes that modify heat production. Insulin is the dominant stimulus of glucose uptake and metabolism, which are fundamental for thermogenic processes. The purpose of this work was to determine the interaction of ambient temperature induced changes in energy expenditure (EE) on the insulin sensitivity of glucose fluxes. Short-term and adaptive responses to thermoneutral temperature (TN, ∼28 °C) and room (laboratory) temperature (RT, ∼22 °C) were studied in mice. This range of temperature does not cause detectable changes in circulating catecholamines or shivering and postabsorptive glucose homeostasis is maintained. We tested the hypothesis that a decrease in EE that occurs with TN causes insulin resistance and that this reduction in insulin action and EE is reversed upon short term (<12h) transition to RT. Insulin-stimulated glucose disposal (Rd) and tissue-specific glucose metabolic index were assessed combining isotopic tracers with hyperinsulinemic-euglycemic clamps. EE and insulin-stimulated Rd are both decreased (∼50%) in TN-adapted vs RT-adapted mice. When RT-adapted mice are switched to TN, EE rapidly decreases and Rd is reduced by ∼50%. TN-adapted mice switched to RT exhibit a rapid increase in EE, but whole-body insulin-stimulated Rd remains at the low rates of TN-adapted mice. In contrast, whole body glycolytic flux rose with EE. This higher EE occurs without increasing glucose uptake from the blood, but rather by diverting glucose from glucose storage to glycolysis. In addition to adaptations in insulin action, ‘insulin-independent’ glucose uptake in brown fat is exquisitely sensitive to thermoregulation. These results show that insulin action adjusts to non-stressful changes in ambient temperature to contribute to the support of body temperature homeostasis without compromising glucose homeostasis.

Deep soft threshold feature separation network for infrared handprint identity recognition and time estimation

With the development of hardware devices, infrared technology has become an important detection method in criminal investigation, military and other fields. Infrared technology can capture the thermal infrared information of targets, achieve efficient and rapid individual identification, provide important technological support for crime investigation, improve case solving rate and public safety level. However, infrared technology still faces some difficulties. Due to factors such as environmental temperature changes and heat transfer, the infrared images captured over time gradually blur, resulting in highly aliased image features and difficulty in extracting effective information. This article addresses this issue by combining the specific tasks of infrared fingerprint identity recognition and time estimation. By analyzing the captured thermal traces to predict the target identity and departure time, a deep soft threshold feature separation (DSTFS) network is proposed. This network effectively improves the accuracy of identity recognition and time estimation by separating the characteristics of identity information and time information. Specifically, this article is based on the ResNet backbone network, extracting common features through shallow residual convolution blocks, extracting corresponding features for different tasks using branch residual convolution blocks, and introducing spatial and channel attention mechanisms to solve the problems of deep blur and feature separation. In order to enhance the model's expressive power, the soft threshold activation function SPRelu was adopted. By dynamically balancing the separation of features to learn weights, the weight balancing problem is solved, and periodic validation is used to evaluate task performance. According to the task requirements, we collected infrared fingerprint images of multiple testers and constructed an infrared hand heat trace dataset with labels such as departure time, gender, hand posture, and identity category as experimental data. Through the analysis of experimental results, our proposed deep feature separation network has better identity prediction results compared to other deep learning models in infrared fingerprint identity recognition and time estimation tasks. At the same time, it performs well in time estimation tasks, with lower error rates and higher stability.

Thermoregulatory consequences of growing up during a heatwave or a cold snap in Japanese quail.

Changes in environmental temperature during development can affect growth, metabolism and temperature tolerance of the offspring. We know little about whether such changes remain to adulthood, which is important to understand the links between climate change, development and fitness. We investigated whether phenotypic consequences of the thermal environment in early life remained in adulthood in two studies on Japanese quail (Coturnix japonica). Birds were raised under simulated heatwave, cold snap or control conditions, from hatching until halfway through the growth period, and then in common garden conditions until reproductively mature. We measured biometric and thermoregulatory [metabolic heat production (MHP), evaporative water and heat loss (EWL, EHL) and body temperature] responses to variation in submaximal air temperature at the end of the thermal acclimation period and in adulthood. Warm birds had lower MHP than control birds at the end of the thermal acclimation period and, in the warmest temperature studied (40°C), also had higher evaporative cooling capacity compared with controls. No analogous responses were recorded in cold birds, although they had higher EWL than controls in all but the highest test temperature. None of the effects found at the end of the heatwave or cold snap period remained until adulthood. This implies that chicks exposed to higher temperatures could be more prepared to counter heat stress as juveniles but that they do not enjoy any advantages of such developmental conditions when facing high temperatures as adults. Conversely, cold temperature does not seem to confer any priming effects in adolescence.

Disentangling drivers of temporal changes in urban pond macroinvertebrate diversity

Ponds are key elements for ecosystem functions in urban areas. However, little is known about pond biodiversity changes over time and the drivers underlying such changes. Here, we tested whether past species assemblages, land cover and pond environmental change influence pond macroinvertebrate species richness and temporal beta diversity. We also compared spatial and temporal beta diversity, and investigated species-specific colonization and extinction rates over time. We sampled for presence of Odonata and Trichoptera (larvae), and Coleoptera and Hemiptera (larvae and adults) species in 30 ponds in Stockholm, Sweden, in 2014 and 2019. Past species richness was the best predictor of current species richness, whereas temporal changes in land cover and pond environment were not significantly related to current species richness. No correlations between temporal beta diversity and land cover or pond environmental changes were detected. However, ponds showed large changes in their temporal beta diversity, with equal contributions from species gains and losses. The probability of species colonizing and going extinct from ponds revealed that more common species were more likely to colonize a pond, while uncommon species were more likely to go extinct in a pond. Within our 5-year study, we found (i) highly similar spatial and temporal beta diversity, (ii) that past species richness is a good predictor of current species richness; however, the same does not hold true for species composition. The high dynamics of urban pond communities suggest that a large number of ponds are required to maintain high species richness at a landscape level.

Optimization of a district heating system coupled with a deep open-loop geothermal well and heat pumps

The technical and economic aspects of a district heating system coupled with a deep open-loop geothermal well (DOLGW) and heat pumps were studied in the paper. A typical configuration of such a geothermal district heating system was proposed, and the corresponding thermal analysis was carried out. Based on the data from the DOLGW heat extraction test conducted in Hebei, China, the optimal size selections of heat pumps, plate heat exchangers, and terminal heat radiators for such a system was analyzed theoretically. In addition, an optimal control scheme for such a system with the change of environmental temperature or heating load were given.

A temperature-independent model of dual calibration standards for onsite and point-of-care quantification analyses via electrophoresis titration chip

Electrophoresis titration chip (ETC) is a versatile tool for onsite and point-of-care quantification analyses because it affords naked-eye detection and a straightforward quantification format. However, it is vulnerable to changes in environmental temperature, which regulates the electrophoretic migration by affecting the ion mobility and the target recognition by influencing the enzyme activity. Therefore, the quantification accuracy of the ETC tests was severely compromised.Rather than using the dry bath or heating/cooling units, we proposed a facile model of dual calibration standards (DCS) to mathematically eliminate the effects of temperature on quantification accuracy. To verify our model, we deployed the ETC device at different temperatures ranging from 5 to 40 °C. We further utilized the DCS-ETC to determine the protein content and uric acid concentration in real samples outside the laboratory. All the experimental results showed that our model significantly stabilized the quantification recovery from 35.31-153.44 % to 99.38–103.44 % for protein titration; the recovery of uric acid titration is also stable at 96.25–106.42 %, suggesting the enhanced robustness of the ETC tests.Therefore, DCS-ETC is a field-deployable test that can offer reliable quantification performance without extra equipment for temperature control. We envision that it is promising to be used for onsite applications, including food safety control and disease diagnostics.

An alumina ceramic inspired high temperature wireless chipless sensor for harsh environment

This paper proposes a wireless passive temperature sensor. The sensitive material as the substrate of the sensor employs an alumina ceramic. The resonator of the sensor is a split ring, which is printed with copper on the substrate. The dimensions of the split ring resonator are optimized to improve the temperature sensitivity of the sensor. A change in environment temperature will lead to a change in the dielectric constant of the sensitive material, thus resulting in the shift of the resonant frequency of the sensor. The variation range of the dielectric constant of the sensitive material is 10.17–11.2 corresponding to 500–1000 °C. The resonant frequency of the signal backscattered from the sensor is obtained through the simulation software with HFSS. The simulation results show that the resonant frequency of the sensor decreases from 5.84 to 5.56 GHz with temperature increasing from 500 to 1000 °C and the temperature sensitivity is 0.56 MHz/°C. The high temperature sensor has the characteristics of wireless and passive operation, low profile, and high sensitivity. It can be widely adapted to a variety of high temperature and harsh environments.

The influence of high-temperature frequency variation on the life-history traits of pyridaben-sensitive and -resistant strains of Tetranychus truncatus.

With a generally warming global climate, the number of Tetranychus truncatus specimens in the Hexi region in China has been increasing. As ectotherms, the growth and development of T. truncatus are greatly affected by changes in environmental temperature. The effect of heatwaves on organisms depends on a delicate balance between damage and repair periods. Therefore, we simulated nine patterns of periodically recurring changes in the frequency of high-temperature days using an intraday gradual temperature change model to study and compare the effects on the development and reproduction of pyridaben-sensitive and -resistant strains of T. truncatus. The results showed that the influence of the frequency of high-temperature days on developmental stages, longevity and fecundity was different between the two strains. The egg and immature stages of the sensitive strain were all affected by hot days, whereas the adult stage was less affected by the frequency. The egg stage of the resistant strain was less affected; it was mainly affected in the immature and adult stages. Under the moderate condition of increasing the proportion of days at normal temperature, the longevity of the resistant strain gradually increased and reached a maximum at a 1:3 frequency, and then it decreased with the increase in high-temperature days. The longevity of the sensitive strain was less affected by frequency, and there was no significant difference between most treatment and control groups. In addition, both sensitive and resistant strains were able to complete growth and development under all nine frequencies of high-temperature days, but the reproductive rate was lower than it was at normal temperatures, indicating that both strains of T. truncatus adapted to high temperatures at the expense of reduced reproduction rates. This lays a key theoretical foundation for predicting the occurrence of agricultural pest populations under the background of climate warming and developing appropriate control strategies.

Analysis of Changes in Environmental Temperature and Humidity and Exploration of Protection Strategies for Ancient Building Groups

Analysis of Changes in Environmental Temperature and Humidity and Exploration of Protection Strategies for Ancient Building Groups

Alterations in mitochondrial structure and function in response to environmental temperature changes in Apostichopus japonicus

Global temperatures have risen as a result of climate change, and the resulting warmer seawater will exert physiological stresses on many aquatic animals, including Apostichopus japonicus. It has been suggested that the sensitivity of aquatic poikilothermal animals to climate change is closely related to mitochondrial function. Therefore, understanding the interaction between elevated temperature and mitochondrial functioning is key to characterizing organisms’ responses to heat stress. However, little is known about the mitochondrial response to heat stress in A. japonicus. In this work, we investigated the morphological and functional changes of A. japonicus mitochondria under three representative temperatures, control temperature (18 °C), aestivation temperature (25 °C) and heat stress temperature (32 °C) temperatures using transmission electron microscopy (TEM) observation of mitochondrial morphology combined with proteomics and metabolomics techniques. The results showed that the mitochondrial morphology of A. japonicus was altered, with decreases in the number of mitochondrial cristae at 25 °C and mitochondrial lysis, fracture, and vacuolization at 32 °C. Proteomic and metabolomic analyses revealed 103 differentially expressed proteins and 161 differential metabolites at 25 °C. At 32 °C, the levels of 214 proteins and 172 metabolites were significantly altered. These proteins and metabolites were involved in the tricarboxylic acid (TCA) cycle, substance transport, membrane potential homeostasis, anti-stress processes, mitochondrial autophagy, and apoptosis. Furthermore, a hypothetical network of proteins and metabolites in A. japonicus mitochondria in response to temperature changes was constructed based on proteomic and metabolomic data. These results suggest that the dynamic regulation of mitochondrial energy metabolism, resistance to oxidative stress, autophagy, apoptosis, and mitochondrial morphology in A. japonicus may play important roles in the response to elevated temperatures. In summary, this study describes the response of A. japonicus mitochondria to temperature changes from the perspectives of morphology, proteins, and metabolites, which provided a better understanding the mechanisms of mitochondrial regulation under environment stress in marine echinoderms.

Managing genomic diversity in conservation programs of Chinese domestic chickens

BackgroundEffective conservation and utilization of farm animals are fundamental for realizing sustainable increases in food production. In situ and ex situ conservation are the two main strategies that are currently used to protect the genetic integrity of Chinese domestic chicken breeds. However, genomic diversity and population structure have not been compared in these conserved populations.ResultsThree hundred and sixty-one individuals from three Chinese domestic chicken breeds were collected from populations conserved in situ and ex situ and genotyped using genotyping-by-sequencing (GBS). First, we used different parameters based on heterozygosity, genomic inbreeding, and linkage disequilibrium to estimate the genomic diversity of these populations, and applied principal component analysis (PCA), neighbor-joining tree, and ADMIXTURE to analyze population structure. We found that the small ex situ conserved populations, which have been maintained in controlled environments, retained less genetic diversity than the in situ conserved populations. In addition, genetic differentiation was detected between the in situ and ex situ conserved populations of the same breed. Next, we analyzed signatures of selection using three statistical methods (fixation index (FST), nucleotide diversity (Pi), and cross-population extended haplotype homozygosity (XP-EHH) to study the genetic footprints that underlie the differentiation between in situ and ex situ conserved populations. We concluded that, in these small populations, differentiation might be caused by genetic drift or by mutations from the original populations. The differentiation observed in the population of Beijing You chicken probably reflects adaptation to environmental changes in temperature and humidity that the animals faced when they were moved from their place of origin to the new site for ex situ conservation.ConclusionsConservation programs of three Chinese domestic chicken breeds have maintained their genomic diversity to a sustainable degree. The small ex situ conserved populations, which are maintained in controlled environments, retain less genetic diversity than populations conserved in situ. In addition, the transfer of populations from their place of origin to another site for conservation purposes results in genetic differentiation, which may be caused by genetic drift or adaptation. This study provides a basis for further optimization of in situ and ex situ conservation programs for domestic chicken breeds in China.

High sensitivity temperature and gas pressure sensor based on PDMS sealed tapered hollow-core fiber

We use hollow core fiber (HCF) and single mode fiber (SMF) fusion splicing to form a sensor with a “SMF-HCF-SMF” structure. To improve the sensitivity of the sensor, the HCF is tapered and stretched to a waist diameter of 10 μm. Then, in order to further improve the sensitivity of the sensor to temperature and pressure, as well as the mechanical strength of the sensor, we coated a layer of polydimethylsiloxane (PDMS) thin film on the surface of the tapered HCF. When light propagates in the sensor, an anti-resonant reflection optical waveguide (ARROW) mechanism is formed. Due to the excellent thermal sensitivity and elasticity of PDMS, the transmission spectrum of the sensor will significantly drift due to changes in environmental temperature and gas pressure, achieving temperature and pressure measurement. The experimental results show that the maximum temperature and gas pressure sensitivity of the sensor reach −3.2321 nm/℃ and −22.80 nm/MPa, respectively. In addition, the sensor also has good repeatability and stability, as well as short response time. It has certain application prospects in the field of high sensitivity temperature and gas pressure sensing measurement.

Phenomenological-based semi-physical model characterizing the central metabolic response of Escherichia coli to changes in batch culture temperature

This work presents a phenomenological-based semi-physical model characterizing the E. coli response to environmental temperature changes in batch cultures. The proposed model is based on a general description of the mass balance of the central metabolism and an energy balance that focuses on key parameters. This model can represent changes in specific growth rate and alteration to the mass flows of the central metabolism. Moreover, the model can make predictions regarding microorganism growth, respiratory rate, glucose consumption, acetate accumulation, and heat exchange between E. coli and its environment at several different temperatures. The fit and validation of the model was assessed against published data obtained under aerobic growth conditions in agitated tank bioreactor cultures. Our assessment of this model indicates that mass and energy balances can predict global changes in E. coli metabolism that are associated with different temperature conditions and differences in heat flow between bacteria and the environment. This model can be used to correlate environmental conditions with experimental bioreactor parameters and simulate microorganismal response during the design of temperature control systems for bioreactors and can even be used as a base model to represent heat-inducible E. coli strains used in recombinant protein production.

The molecular composition of leaf lipids changes with seasonal gradients in temperature and light among deciduous and evergreen trees in a sub-humid ecosystem

Forest canopy structure is a fundamental ecosystem attribute affecting regional and global climate through primary production by CO2 drawdown and evapotranspirative feedback. Environmental changes in temperature and light affect leaf physiology and thus canopy functioning. Leaf physiological changes may be reflected in expressed chemical compounds (e.g., leaf lipid biomarkers), that offer opportunities to characterize and quantify climatic effects on plant canopies in the present and the past. To assess this possibility, we systematically investigated the lipids from leaves of deciduous angiosperm (Quercus buckleyi, April–October 2019) and evergreen gymnosperm (Juniperus ashei, April–October 2019 and January 2020) tree species at monthly sampling intervals over one growing season in a natural sub-humid ecosystem of central Texas. Fatty acid unsaturation in Q. buckleyi and J. ashei leaves was negatively correlated with air temperature. The average chain lengths of leaf wax n-alkanols of Q. buckleyi were strongly correlated with leaf area index (LAI) and absorbed photosynthetically active radiation (APAR) (r2 > 0.5). The stigmasterol/β-sitosterol ratio was correlated with light transmittance in the canopy of Q. buckleyi, with values of the sterol ratio three-fold higher in shaded leaves than in sparse canopies. The observed seasonal changes in leaf lipid molecular composition and chain-lengths might be related to their biosynthetic responses to temperature and light stresses. Finally, we developed multi-lipid regression models resolving seasonal differences in temperature, LAI, and APAR. We posit that the specific lipid biosynthetic responses to variations in temperature and light are a basis for reconstructing terrestrial paleoenvironmental changes.

Heat supply pipeline detection based on temperature gradient data of shallow ground

Heat supply pipelines are a critical component of urban infrastructure, with numerous underground projects relying on this crucial and often concealed system. In cases where blueprints are unavailable, it is crucial to determine the precise location and depth of heat supply pipelines. One of the most straightforward methods for detecting these pipelines is measuring underground temperature. Temperature gradient data is less sensitive to environmental temperature changes for detecting shallow heat sources than temperature data. This paper presents an extension of the correlation imaging method to handle temperature gradient data. This method can accurately determine the horizontal center position and the depth of the heat source. This temperature tomographic approach is reliable and has a high resolution, as evidenced by both synthetic and field examples.

Terrestrial amphibians respond to rapidly changing temperatures with individual plasticity of exploratory behaviour

Terrestrial ectotherms react to acute changes in environmental temperatures by adjusting their behaviour. Evaluating the adaptive potential of these behavioural adjustments requires information on their repeatability and plasticity. We examined behavioural response (exploration) to acute temperature change in two amphibian taxa, alpine (Ichthyosaura alpestris) and smooth (Lissotriton vulgaris) newts. These responses were investigated at both population and individual levels under multiple thermal contexts (dimensions), represented by the direction and range of changing temperature and rearing thermal regimes. Population-level analyses showed species-specific, non-additive effects of direction and range of temperature change on acute thermal reaction norms for exploration, but explained only a low amount (7–23%) of total variation in exploration. In contrast, within- and among-individual variation in acute thermal reaction norm parameters explained 42–50% of total variation in the examined trait. Although immediate thermal responses varied among individuals (repeatability = 0.07 to 0.53), they were largely shaped by environmental contexts during repeated trials. We conclude that these amphibians respond to acute temperature change through individual plasticity of behavioural traits. A repeated-measures approach under multiple thermal contexts will be needed to identify the selective and plastic potential of behavioural responses used by juvenile newts and perhaps other ectotherm taxa to cope with rapidly changing environmental temperatures.

Thermal sensitivity of field metabolic rate predicts differential futures for bluefin tuna juveniles across the Atlantic Ocean

Changing environmental temperatures impact the physiological performance of fishes, and consequently their distributions. A mechanistic understanding of the linkages between experienced temperature and the physiological response expressed within complex natural environments is often lacking, hampering efforts to project impacts especially when future conditions exceed previous experience. In this study, we use natural chemical tracers to determine the individual experienced temperatures and expressed field metabolic rates of Atlantic bluefin tuna (Thunnus thynnus) during their first year of life. Our findings reveal that the tuna exhibit a preference for temperatures 2–4 °C lower than those that maximise field metabolic rates, thereby avoiding temperatures warm enough to limit metabolic performance. Based on current IPCC projections, our results indicate that historically-important spawning and nursery grounds for bluefin tuna will become thermally limiting due to warming within the next 50 years. However, limiting global warming to below 2 °C would preserve habitat conditions in the Mediterranean Sea for this species. Our approach, which is based on field observations, provides predictions of animal performance and behaviour that are not constrained by laboratory conditions, and can be extended to any marine teleost species for which otoliths are available.