Environmental Risk Assessment Research Articles

Small-Intensity Rainfall Triggers Greater Contamination of Rubber-Derived Chemicals in Road Stormwater Runoff from Various Functional Areas in Megalopolis Cities.

Rubber-derived chemicals (RDCs) originating from tire and road wear particles are transported into road stormwater runoff, potentially threatening organisms in receiving watersheds. However, there is a lack of knowledge on time variation of novel RDCs in runoff, limiting initial rainwater treatment and subsequent rainwater resource utilization. In this study, we investigated the levels and time-concentration profiles of 35 target RDCs in road stormwater runoff from eight functional areas in the Greater Bay Area, South China. The results showed that the total concentrations of RDCs were the highest on the expressway compared with other seven functional areas. N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD), 6PPD-quinone, benzothiazole, and 1,3-diphenylguanidine were the top four highlighted RDCs (ND-228840 ng/L). Seasonal and spatial differences revealed higher RDC concentrations in the dry season as well as in less-developed regions. A lag effect of reaching RDC peak concentrations in road stormwater runoff was revealed, with a lag time of 10-90 min on expressways. Small-intensity rainfall triggers greater contamination of rubber-derived chemicals in road stormwater runoff. Environmental risk assessment indicated that 35% of the RDCs posed a high risk, especially PPD-quinones (risk quotient up to 2663). Our findings contribute to a better understanding of managing road stormwater runoff for RDC pollution.

Plant characterization of insect-protected soybean

Insect-protected soybean (SIP) that produces the Cry1A.105 and Cry2Ab2 insecticidal crystal proteins has been developed to provide protection from feeding damage caused by targeted lepidopteran insect pests. Typically, as part of environmental risk assessment (ERA), plant characterization is conducted, and the data submitted to regulatory agencies prior to commercialization of genetically modified (GM) crops. The objectives of this research were to: (a) compare soybean with and without the SIP trait in plant characterization field trials designed to fulfill requirements for submissions to global regulatory agencies and address China-specific considerations and (b) compare risk assessment conclusions across regions and the methodologies used in the field trials. The soybean with and without the SIP trait in temperate, tropical, and subtropical germplasm were planted in replicated multi-location trials in the USA (in 2012 and 2018) and Brazil (in 2013/2014 and 2017/2018). Agronomic, phenotypic, plant competitiveness, and survival characteristics were assessed for soybean entries with and without the SIP trait. Regardless of genetic background, growing region, season, or testing methodology, the risk assessment conclusions were the same: the evaluated insect-protected soybean did not differ from conventional soybean in evaluated agronomic, phenotypic, competitiveness, and survival characteristics indicating no change in plant pest/weed potential. These results reinforce the concept of data transportability across global regions, different seasons, germplasm, and methodologies that should be considered when assessing environmental risks of GM crops.

Macrophage polarization as a novel endpoint for assessing combined risk of phthalate esters

Combined exposure to phthalate esters (PAEs) has garnered increasing attention due to potential synergistic effects on human health. This study aimed to develop an in vitro model using human macrophages to evaluate the combined toxicity of PAEs and explore the underlying mechanisms. A high-throughput screening system was engineered by expressing a PPRE-eGFP reporter in THP-1 monocytes to monitor macrophage polarization upon PAEs exposure. Individual PAEs exhibited varied inhibitory effects on M2 macrophage polarization, with mono(2-ethylhexyl) phthalate (MEHP) being the most potent. Isobologram analysis revealed additive interactions when MEHP was combined with other PAEs, resulting in more pronounced suppression of M2 markers compared to individual compounds. Mechanistic studies suggested PAEs may exert effects by modulating PPARγ activity to inhibit M2 polarization. Notably, an equimolar mixture of six PAEs showed additive inhibition of M2 markers. In vivo experiments corroborated the combined hepatotoxic effects, with mice exposed to a PAEs mixture exhibiting reduced liver weight, dyslipidemia, and decreased hepatic M2 macrophages compared to DEHP alone. Transcriptome analysis highlighted disruptions in PPAR signaling, and distinct pathway alterations on cholesterol metabolism in the mixture group. Collectively, these findings underscore the importance of evaluating mixture effects and provide a novel approach for hazard assessment of combined PAEs exposure with implications for environmental health risk assessment.

Active ingredient identification and purification of organosilicon spray adjuvant and its uptake and translocation in rice (Oryza sativa L.).

The assessment of residue, absorption, conduction, and degradation of agricultural organosilicon surfactants in the environment is hindered by the lack of information on active ingredients and corresponding quantitative standards for organosilicon spray adjuvants. The spray adjuvant 'Jiexiaoli,' a primary organosilicon spray agent in China, was identified as hydroxy (polyethylene) propyl-heptamethyl trisiloxane (TSS-H) with 3-15 ethoxy (EO) groups. Purification of TSS-H was achieved through semi-preparative separation using high-performance liquid chromatography (HPLC), resulting in TSS-H purity exceeding 96%. An accurate residual detection method for nine oligomers (4-12 EO) of TSS-H in rice roots, stems, leaves, and culture solution samples was developed using HPLC tandem high-resolution mass spectrometry (HPLC-HRMS). Recoveries for nine oligomers of TSS-H in the four matrices ranged from 80.22% to 104.01%. Foliar application experiments demonstrated that TSS-H did not transfer from the upper to the lower parts of the rice plant. The half-lives of each oligomer (4-12 EO) in leaves were less than 3.21 days. Root application experiments revealed a root concentration factor (RCF) ranging from 0.20 to 0.56, a biological enrichment factor (BCF) ranging from 0.36 to 0.68, a transpiration factor (TSCF) ranging from 0.069 to 0.086, and a transport factor (TF) ranging from 0.08 to 0.43. These results indicated that TSS-H could be absorbed by rice roots and conducted to the above-ground parts of rice plants. This study fills the data gap in the environmental risk and food safety assessment of agricultural silicone spray adjuvants. © 2024 Society of Chemical Industry.

Prediction of municipal solid waste generation and environmental risk assessment of heavy metal based on long short term memory

Prediction of municipal solid waste generation and environmental risk assessment of heavy metal based on long short term memory

Comparative investigation on composting and pyrolysis of swine manure: Heavy metals transformation, nitrogen immobilization and integrated environmental risk assessment

Harmless treatment of livestock manure has been a pressing issue due to its contamination of heavy metals and volatilization of nitrogen(N)-containing components during processing. In this paper, the chemical species transformation, leaching characteristics of Cr, Mn, Zn, Cu and conversion of amine N and inorganic N during composting and pyrolysis of swine manure (400°C, 500°C, 600°C and 700°C) were comparatively investigated, and their integrated environmental risk (η) was assessed. According to the ultimate and proximate analysis, pyrolyzed char exhibited better aromaticity and richer functional groups compared with that of compost; Cr, Mn, Zn, Cu were transformed into more stable residual states (F3+F4), and better inhibition of leaching in simulated landfill experiments was shown than compost. However, deamination and cyclocondensation reactions under higher pyrolysis temperatures resulted in N escaping in the form of NH3 and HCN. Considering various pollution coefficients, the integrated ecological risk was reduced by 59.24 % after 600°C pyrolysis, which is the optimal condition for the minimization of the risk of heavy metal toxicity and N volatilization among all the compost and pyrolysis process.

Assessment of potential environmental and human risks for Bisphenol AF contaminant

Bisphenol AF (BPAF) is found in high concentrations in aquatic environments due to the increased use of thermal paper and food packaging. However, there have been relatively few toxicological studies and potential risk assessments of BPAF. In this study, the risk quotient (RQ) and hazard quotient (HQ) of BPAF were derived to present the safety standards for environmental risk management and protection in lakes, rivers, bays, and Italian regions. We applied the species sensitivity distribution (SSD) method based on the previous ecotoxicological data and the results of supplementary toxicity tests on BPAF. From the SSD curves, the hazardous concentration for 5 % of the species (HC5) values for the acute and chronic toxicity data were 464.75 µg/L and 3.59 µg/L, respectively, and the acute- and chronic-based predicted no-effect concentration were derived as 154.92 µg/L and 1.20 µg/L, respectively. The acute-based RQ (RQA)values of BPAF in all regions were negligible (RQ < 0.1). The chronic-based RQ (RQC) in the Xitang River (XR) and the Central Italy (CI) showed a considerably high ecological risk (12.77 and 1.29) and the Hangzhou Bay (0.21), the South and North Italy (0.79 and 0.27), and the Tamagawa River (0.13) had a medium ecological risk (0.1 < RQ < 1.0). However, the HQ values based on the tolerable daily intake for BPAF over all age groups in these regions was < 0.1, indicating the low health risk. Nonetheless, the result of this study indicates that BPAF contamination is serious in XR and CI, and their use and emissions require continuous monitoring.

The impact of green credit guidelines on environmental performance: Firm-level evidence from China

The Green Credit Guidelines (GCG) regulates the environmental behaviour of firms with high pollution, high energy consumption, and surplus production capacity (known as “two high and one surplus”, THOS) through environmental and social risk assessment and credit management, thereby improving firms' environmental performance. Adopting the panel data of 461 Chinese A-share listed firms from 2008 to 2020 and the Difference-in-Differences (DID) model, this paper employs the promulgation of GCG in China as a quasi-natural experiment to investigate the effect of GCG on THOS firms' environmental performance and its potential mechanisms. The main findings are reported as follows. Firstly, the promulgation of GCG significantly improves the THOS firms' environmental performance. Secondly, after dividing the firms' environmental performance into the environmental strengths and concerns, the policy effect is stronger on environmental strengths that focus on process control. Thirdly, the firms' environmental performance improvement effect of the GCG is mainly achieved by promoting green innovation and exacerbating financing constraints. Finally, the improvement effect of the GCG on firms' environmental performance is more pronounced in firms with weaker external monitoring, environmental information disclosure and lower capital dependence.

Comprehensive Analysis and Environmental Risk Assessment of Benzotriazoles in Airport Stormwater: A HS-SPME-GC × GC-TOF-MS-Based Procedure as a Tool for Sustainable Airport Runoff Water Management

Despite the numerous benefits of intensive air transport development, many activities associated with the operation of airports contribute to environmental pollution. The purpose of this research was the development, optimization, and validation of a headspace–solid-phase microextraction–comprehensive two-dimensional gas chromatography–time of flight–mass spectrometry (HS-SPME-GC × GC-TOF-MS)-based procedure for determining anti-corrosive compounds in airport stormwater. Optimized HS-SPME conditions include: 45 min extraction time, 100 °C temperature, 1.0 g salt addition, and 10 min desorption time at 270 °C. The developed procedure is sensitive, selective, accurate (recoveries ≥ 80.0%), and precise (the coefficient of variation (CV) ≤ 14.9%), making it a highly suitable tool for extensive airport stormwater quality monitoring. The validated analytical protocol was successfully used to detect pollutants, including 1H-BT, 4-MeBT, 5-MeBT, and 5,6-diMe-1H-BT, in stormwater from various European airports with different flight capacities. Throughout the sampling period at the investigated airports, 1H-benzotriazole was found in the highest concentrations, ranging from below the MQL to 467 mg/L. An ecotoxicological risk assessment revealed that 69% of the sites exhibited high risk levels (Risk Quotient ≥ 1). The developed procedure and carried out environmental risk assessments of benzotriazoles in airport stormwater enable an evidence-based approach to sustainable airport stormwater management.

In Vitro Toxicity and Modeling Reveal Nanoplastic Effects on Marine Bivalves.

Nanoplastics (NPs) represent a growing concern for global environmental health, particularly in marine ecosystems where they predominantly accumulate. The impact of NPs on marine benthic organisms, such as bivalves, raises critical questions regarding ecological integrity and food safety. Traditional methods for assessing NP toxicity are often limited by their time-intensive nature and ethical considerations. Herein, we explore the toxicological effects of NPs on the marine bivalve Ruditapes philippinarum, employing a combination of in vitro cellular assays and advanced modeling techniques. Results indicate a range of adverse effects at the organismal level, including growth inhibition (69.5-108%), oxidative stress, lipid peroxidation, and DNA damage in bivalves, following exposure to NPs at concentrations in the range of 1.6 × 109-1.6 × 1011 particles/mL (p/mL). Interestingly, the growth inhibition predicted by models (54.7-104%), based on in vitro cellular proliferation assays, shows strong agreement with the in vivo outcomes of NP exposure. Furthermore, we establish a clear correlation between cytotoxicity observed in vitro and the toxicological responses at the organismal level. Taken together, this work suggests that the integration of computational modeling with in vitro toxicity assays can predict the detrimental effects of NPs on bivalves, offering insightful references for assessing the environmental risk assessment of NPs in marine benthic ecosystems.

Pesticides in water and sediments from natural protected areas of Spain and their associated ecological risk

In the last years, issues related to intensive agriculture have been found in protected areas potentially harming wildlife. This study aimed to analyze a wide range of pesticides in water and sediments of two protected areas namely Doñana Natural Park (DNP) and Tablas de Daimiel National Park (TDNP) performing an environmental risk assessment in order to highlight potential risks to living organisms derived from pesticide burden. Higher pesticide load was found in DNP than TDNP with similar distribution profiles, with pyrethroid insecticides (PYRs) the main detected class. Particularly problematic are two PYRs, cyhalothrin and fenvalerate, which were detected at high concentrations that can pose a high risk to aquatic organisms. In addition, despite being detected at lower concentrations, the presence of chlorpyrifos, cypermethrin, and permethrin in water, and of chlorpyrifos, dicofol, and diflufenican in sediments, must be taken into account due to their potential risks for aquatic organisms. Moreover, some banned pesticides such as dimethoate, terbutryn, diazinon, and tricyclazol were detected in water at levels which deserve further investigation to assess their potential sources, including potential illegal practices.

Food, bodies, health (risks): the biopolitics of organic materiality testing in the context of diet-associated health risk management practices

This article analyses the apparatus of practices dedicated to reducing long-term diet-associated health risks so as to question how risk is framed and worked upon in everyday risk governance contexts. In 2020–2021, I conducted 10 semi-structured interviews with public & environmental health researchers and programme managers at both federal (Canada) and provincial (Quebec) levels, as well as with clinical practitioners (clinical physician, dietician). I also used these interviews and the information provided by my interviewees to gather a corpus of materials comprised of clinical protocols, empirical guidelines, governmental screening programmes, and more. In the analysis, I contrast testing practices dedicated to preventing and controlling chronic conditions associated with food ingestion such as type 2 diabetes, with others dedicated to predicting and preventing health conditions associated with the ingestion of pesticides and contaminants. I use Foucauldian discourse analysis methods as well as Sheila Jasanoff’s (1999) “songlines of risk” in her analysis of environmental risk assessment practices to analyse how the risk mitigation practices under study integrate different approaches to risk and thus different ways of caring for health and bodies. This leads me to a discussion on the biopolitical approach to risk and health that informs these practices and the apparatus they constitute, contributing to orienting where the onus of the responsibility lies when it comes to managing or preventing diet-associated health conditions. I argue that the apparatus plays a role in invisibilizing the environmental factors in disease causation and reinforcing the individualisation of health (responsibility) rather than its collectivisation.

Exudates of Microcystis aeruginosa on oxidative stress and inflammatory responses in gills of Sinocyclocheilus grahami

Early cyanobacterial blooms studies observed that exposure to blue-green algae led to fish gills impairment. The objective of this work was to evaluate the toxic mechanisms of exudates of Microcystis aeruginosa (MaE) on fish gills. In this study, the toxic mechanism of MaE (2×106 cells/mL) and one of its main components phytosphingosine (PHS) with two concentrations 2.9 ng/mL and 145 ng/mL were conducted by integrating histopathology, biochemical biomarkers, and transcriptomics techniques in Sinocyclocheilus grahami (S. grahami) for 96 h exposure. Damaged gill tissue with epithelial hyperplasia and hypertrophy, remarkable Na+/K+-ATPase (NKA) enzyme activity, disrupted the redox homeostats including lipid peroxidation and inflammatory responses were observed in the fish of MaE exposure group. Compare to MaE exposure, two concentrations of PHS exposure appeared to be a trend of lower degree of tissue damage, NKA activity and oxidative stress, but induced obviously lipid metabolism disorder with higher triglycerides, total cholesterol and total bile acid, which might be responsible for inflammation responses in fish gill. By transcriptome analysis, MaE exposure were primarily enriched in pathways related to gill function and immune response. PHS exposure, with higher number of differentially expressed genes (DEGs), were enriched in Toll-like receptor (TLR), Mitogen-Activated Protein Kinase (MAPK) and NOD-like receptor protein 3 (NLRP3) pathways. We concluded that MaE and PHS were induced the inflammatory responses, with oxidative stress-induced inflammation for MaE exposure but lipid metabolism disorder-induced inflammation for PHS exposure. The present study provided two toxin-induced gill inflammation response pathways under cyanobacterial blooms, which could be a scientific basis for the ecological and health risk assessment in the aquatic environment.

Elucidating the Interaction of Indole-3-Propionic Acid and Calf Thymus DNA: Multispectroscopic and Computational Modeling Approaches.

Indole-3-propionic acid (IPA) is a plant growth regulator with good specificity and long action. IPA may be harmful to human health because of its accumulation in vegetables and fruits. Therefore, in this study, the properties of the interaction between calf thymus DNA (ctDNA) and IPA were systematically explored using multispectroscopic and computational modeling approaches. Analysis of fluorescence spectra showed that IPA binding to ctDNA to spontaneously form a complex was mainly driven by hydrogen bonds and hydrophobic interaction. DNA melting analysis, viscosity analysis, DNA cleavage study, and circular dichroism measurement revealed the groove binding of IPA to ctDNA and showed that the binding did not significantly change ctDNA confirmation. Furthermore, molecular docking found that IPA attached in the A-T rich minor groove region of the DNA. Molecular dynamics simulation showed that DNA and IPA formed a stable complex and IPA caused slight fluctuations for the residues at the binding site. Gel electrophoresis experiments showed that IPA did not significantly disrupt the DNA structure. These findings may provide useful information on the potential toxicological effects and environmental risk assessments of IPA residue in food at the molecular level.

Terramechanics analysis of climbing behavior of wheel against rigid obstacle on soft terrain

In the development of lunar and planetary exploration rovers, risk assessment in on-site environments comprising fine regoliths and unevenly distributed rocks is essential. To prevent a rover from being stuck during its operation, one must understand the behavior of its wheel when it climbs over rigid obstacles in off-road environments. In this study, we apply an extended terramechanics model, which can reasonably describe the interaction between soft ground and vehicles, to analyze the obstacle-climbing behavior of rigid wheels. To describe the interaction between the wheel and a hard obstacle, we combine the penalty method with the extended terramechanics model. Subsequently, we verify the effectiveness of the proposed method by comparing its results with those of a traversing single wheel obtained from a laboratory experiment. Furthermore, we use the model to perform a multibody dynamics analysis on a simple rover, where its applicability to the examination of the overall performance of obstacle climbing is demonstrated.

Progress in the environmental risk assessment of plant protection products in Brazil: An overview of aquatic organism proposals

AbstractSince 2019, the Brazilian Institute of Environment and Renewable Natural Resources (IBAMA) has actively developed pesticide environmental risk assessment (ERA) frameworks adapted to Brazil's specific ecological contexts. This endeavor, supported by funding from the Brazilian Ministry of Justice and in partnership with academic institutions, has led to a concerted effort to establish ERA protocols for various taxa, including birds and mammals, soil organisms, aquatic organisms, and reptiles and amphibians. The outcomes of this initiative were conveyed in two workshops held in February and November 2023, during which the agency communicated its findings to the technical‐regulatory community. This article represents one of three articles that provide more detailed insights into the ERA propositions for all taxa. In this article, we summarize the proposals for aquatic organisms presented and discussed during the workshops, which can be used as an informational source by the technical‐regulatory community. Integr Environ Assess Manag 2024;00:1–6. © 2024 The Author(s). Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology &amp; Chemistry (SETAC).

Cadmium and ketoprofen accumulation influences aquatic ecosystem demonstrated using in-vivo zebrafish model

The growing concern about pollution and toxicity in aquatic as well as terrestrial organisms is predominantly caused due to waterborne exposure and poses a risk to environmental systems and human health. This study addresses the co-toxic effects of cadmium (Cd) and ketoprofen (KPF), representing heavy metal and pharmaceutical discharge pollutants, respectively, in aquatic ecosystems. A 96-h acute toxicity assessment was conducted using zebrafish embryos. The results indicated that high dosages of KPF (10, 15, and 100 µg/mL) and Cd (10 and 15 µg/mL) reduced survivability and caused concentration-dependent deformities such as scoliosis and yolk sac edema. These findings highlight the potential defects in development and metabolism, as evidenced by hemolysis tests demonstrating dose-dependent effects on blood cell integrity. Furthermore, this study employs adult zebrafish for a 42-day chronic exposure to Cd and KPF (10 and 100 µg/L) alone or combined (10 + 10 and 100 + 100 µg/L) to assess organ-specific Cd and KPF accumulation in tissue samples. Organ-specific accumulation patterns underscore complex interactions impacting respiratory, metabolic, and detoxification functions. Prolonged exposure induces reactive oxygen species formation, compromising antioxidant defense systems. Histological examinations reveal structural changes in gills, gastrointestinal, kidney, and liver tissues, suggesting impairments in respiratory, osmoregulatory, nutritional, and immune functions. This study emphasizes the importance of conducting extensive research on co-toxic effects to assist with environmental risk assessments and safeguard human health and aquatic ecosystems.

Environmental risk assessment of coastal dredging based on clustering of meteocean forcing

Dredging and dumping in-situ sediments is a fundamental operation for most coastal engineering projects and coastal defense projects, such as the construction of breakwaters, beach nourishment and land reclamation. Future projections in terms of coastal hazard suggest that coastal protection and land reclamation project will be more and more frequent. In this context, the assessment of the environmental and socio-economic impact of the risk induced by dredging is a fundamental step during both the design stage and the operational management. Most of the standard practices and available risk assessment frameworks rely on the numerical prediction of the sediment plume in the large field driven by coastal circulations forced by tides, winds and waves. In this study, we formulated a new risk assessment framework based on an unsupervised machine learning clustering algorithm, K-means clustering, for generating representative meteocean scenarios subsequently used to force a regional circulation model. Moreover, we introduced three criteria of hazard/risk based on the spatial and temporal evolution of the suspended sediment concentration that explained different environmental impacts and two new methods to synthetically present the risk values. The major improvement of the present framework is that the final probability of risk fully describes the statistics in terms of hydrodynamic and dredging conditions.This framework presents the probability analysis of risk spatial distribution based on representative hydrodynamic conditions and dredging scenarios, which is a major improvement of this study compared with previous risk assessment strategies that were unable to predict quantified dredging risk before field construction. Finally, to demonstrate the potentiality of the risk assessment framework, we applied this methodology to the Hong Kong Water and Pearl River Estuary (China) as a pilot case.

Environmentally Relevant Antibiotic Concentrations Exert Stronger Selection Pressure on River Biofilm Resistomes than AMR-Reservoir Effluents.

Freshwater environments are primary receiving systems of wastewater and effluents, which carry low concentrations of antibiotics and antimicrobial-resistant (AMR) bacteria and genes. Aquatic microbial communities are thus exposed to environmentally relevant concentrations of antibiotics (ERCA) that presumably influence the acquisition and spread of environmental AMR. Here, we analyzed ERCA exposure with and without the additional presence of municipal wastewater treatment plant effluent (W) and swine manure run-off (M) on aquatic biofilm resistomes. Microscopic analyses revealed decreased taxonomic diversity and biofilm structural integrity, while metagenomic analysis revealed an increased abundance of resistance, virulence, and mobile element-related genes at the highest ERCA exposure levels, with less notable impacts observed when solely exposed to W or M effluents. Microbial function predictions indicated increased gene abundance associated with energy and cell membrane metabolism and heavy metal resistance under ERCA conditions. In silico predictions of increased resistance mechanisms did not correlate with observed phenotypic resistance patterns when whole communities were exposed to antimicrobial susceptibility testing. This reveals important insight into the complexity of whole-community coordination of physical and genetic responses to selective pressures. Lastly, the environmental AMR risk assessment of metagenomic data revealed a higher risk score for biofilms grown at sub-MIC antibiotic conditions.

Resource and environmental risk assessment of groundwater well fields in the Beijing-Tianjin-Hebei region

Groundwater in the Beijing-Tianjin-Hebei (BTH) region has been overdrawn for a long time, and groundwater pollution is severe. Groundwater well fields (GWFs) face resource- and environment-related pressures, and their safety is a key issue restricting regional social and economic development. We evaluated the groundwater quality and drinking suitability via the entropy-weighted water quality index and the overall health risks to adults and children with an improved model. Furthermore, the risks to GWF resources were determined based on the groundwater level and its variation. The higher Na+ and NO3− concentrations were mainly due to agricultural and industrial activities. Forty-seven GWFs approached the Class Ⅳ water standard, and 25 and 4 GWFs posed high health risks to adults and children, respectively, suggesting environmental risks. Moreover, the average annual groundwater level in the groundwater depression cone of 2 shallow and 6 deep GWFs decreased by 0.25 and 3.2 m, respectively. These GWFs were substantially threatened by groundwater resource attenuation. The groundwater level of 4 GWFs slowly declined, but a deep groundwater depression cone was not formed. However, the risk to groundwater resources remained high. The resource-related and environmental risks of GWFs in the BTH region were synchronous, but the resource-related risk was higher.